OCT用于合并糖尿病与非糖尿病白内障患者术后黄斑厚度变化观察

目的:通过光学相干断层扫描(OCT)观察合并糖尿病与非糖尿病白内障患者术后黄斑厚度的变化。方法:选取白内障患者78例(78眼)作为研究对象,患者均行超声乳化白内障吸出术,根据是否合并糖尿病分为两组,糖尿病组40例(40眼),非糖尿病组38例(38眼),采用OCT测量两组患者术前、术后1 d、术后1周以及术后1个月时黄斑中心凹厚度的变化情况。结果:非糖尿病组术后1 d、1周及1个月的中心凹区厚度与术前比较,差异无统计学意义(P>0.05),糖尿病组术后1 d、1周黄斑区视网膜厚度与术前相比,差异无统计学意义(P>0.05),术后1个月较术前显著增加,差异有统计学意义(P<0.05);两组术前和术后各时段的内环区、外环区视网膜厚度对比,差异无统计学意义(P>0.05)。结论:手术后,糖尿病白内障患者视网膜厚度较术前明显增加,黄斑中心凹区改变明显,提示糖尿病视网膜病变是引起白内障术后黄斑水肿的高危因素。     

糖尿病对白内障患者术后临床疗效的影响

目的:探讨糖尿病对白内障患者术后临床疗效的影响。方法:选取深圳市龙岗中心医院2020年1月至2021年1月收治的100例2型糖尿病并年龄相关性白内障患者为观察组,另选同期无糖尿病的100例年龄相关性白内障患者为对照组,两组患者的一只眼接受白内障超声乳化手术治疗,另一只眼未接受该手术治疗,测定并比较两组患者视力、黄斑中心凹视网膜厚度差异。结果:术前两组患者的最佳矫正视力、裸眼远视力、黄斑中心凹视网膜厚度数值比较,差异无统计学意义(P>0.05),术后1周、术后1、3、6个月各组手术眼视力均较术前提高,且观察组各时间段的手术眼最佳矫正视力、裸眼远视力均低于对照组,差异均有统计学意义(P<0.05),观察组各时间段手术眼黄斑中心凹视网膜厚度较术前增加且均大于对照组手术眼,差异有统计学意义(P<0.05),对照组手术眼及两组未手术眼间差异无统计学意义(P>0.05)。结论:糖尿病患者白内障超声乳化手术后黄斑中心凹视网膜厚度明显增加,视力改善效果下降,需引起临床足够的重视。     

光学相干断层扫描观察高度近视眼白内障超声乳化术后黄斑区变化

目的观察高度近视眼白内障超声乳化术后黄斑区视网膜的变化及术后视力恢复情况。方法 105例(150眼)白内障患者分为高度近视眼白内障组(A组,56例,80眼)和非高度近视眼白内障组(B组,49例,70眼),分别行超声乳化联合人工晶体植入术。分别于术前和术后1周、1个月和3个月测量患者裸眼视力(UCVA)和最佳矫正视力(BCVA);行光学相干断层扫描,测量黄斑中心凹视网膜神经上皮层厚度(MFT)和黄斑中心凹直径6 mm范围内的黄斑区容积(TMV);计算高、低累积能量复合参数(AECP)。结果 A组和B组术后各时间点UCVA、BCVA和TMV均显著高于术前(P<0.01),而术后各时间点MFT与术前比较差异均无统计学意义(P>0.05)。A组和B组中,高、低AECP患者术后各时间点MFT与术前比较差异均无统计学意义(P>0.05);高、低AECP患者术后各时间点TMV均显著高于术前(P<0.01);高AECP患者术后各时间点MFT和TMV与术前的差值与低AECP患者比较差异均无统计学意义(P>0.05)。术后A组中1眼黄斑区鼻下方发生视网膜神经上皮层浅脱离,A组中3眼、B组中1眼黄斑区中心凹发生微囊样改变。结论高度...     

康柏西普玻璃体腔注射联合激光光凝对糖尿病黄斑水肿患者脉络膜厚度的影响

目的:观察玻璃体腔注射康柏西普联合激光光凝治疗对糖尿病黄斑水肿(DME)患者脉络膜厚度的影响。方法:将光学相干断层扫描、眼底荧光血管造影诊断为糖尿病黄斑水肿的患者56例(63只眼),随机分为两组:联合治疗组30例(32只眼),激光治疗组26例(31只眼)。联合治疗组先给予玻璃体腔注射康柏西普,1周后行黄斑区格栅光凝治疗,激光治疗组行黄斑区格栅治疗。两组中、重度非增殖性糖尿病视网膜病变(NPDR)及增殖性糖尿病视网膜病变(PDR)患者行全视网膜激光光凝术(PRP)治疗。在治疗后1个月,3个月,6个月观察黄斑中心凹下脉络膜厚度(SFCT),同时观察最佳矫正视力(BCVA)及黄斑中心凹厚度(CMT)。分析黄斑中心凹下脉络膜厚度变化与最佳矫正视力和黄斑中心凹厚度变化的关系。结果:术后1个月,3个月,6个月两组的黄斑中心凹下脉络膜厚度与术前比较均降低,差异均具有统计学意义(P<0.05);最佳矫正视力(BCVA)与术前比较均提高,差异均具有统计学意义(P<0.05);CMT与术前比较均降低,差异均具有统计学意义(P<0.05)。术后1个月,联合治疗组SFCT低于单纯激光组,差异具有统计学意义(P<0.05);术后1个月,3个月联合治疗组的BCVA高于单纯激光组,差异具有统计学意义(P<0.05),联合治疗组CMT低于单纯激光组,差异具有统计学意义(P<0.05)。结论:玻璃体腔内注射康柏西普联合激光治疗糖尿病黄斑水肿,短期内脉络膜厚度明显降低,脉络膜厚度的变化可在一定程度上评估糖尿病黄斑水肿的治疗效果。     

术前SD-OCT对白内障术后视力预测的意义和应用价值

目的探讨术前频域-光学相干断层扫描技术（SD-OCT）定量测量黄斑部视网膜对白内障术后视力预测的意义和临床应用价值。方法选择行白内障超声乳化联合人工晶状体植入术的56例老年性白内障患者（56眼）,术前采用SD-OCT测量术眼的黄斑部中心子区视网膜厚度（CST）、黄斑容积（CV）和黄斑容积平均厚度（CAT）,术后记录其最佳矫正视力,采用Pearson相关分析术前CST、CV、CAT与术后最佳矫正视力的相关性。结果术前最佳矫正视力为0.20±0.12,术后1周最佳矫正视力为0.54±0.19,术后视力较术前明显提高,差异有统计学意义（P＜0.05）。白内障术后1周最佳矫正视力与术前CV和CAT呈正相关（r=0.398和0.399,均P＜0.01）,与术前CST无相关性（r=0.074,P＞0.05）。结论术前SD-OCT对黄斑部视网膜厚度的测量对白内障术后的最佳矫正视力有一定的预估作用,可作为白内障术前准确评估黄斑部视网膜功能以及术后视力的重要方法。     

后巩膜加固术治疗进行性近视的临床研究

目的探讨后巩膜加固术治疗进行性近视的疗效。方法将50例（92眼）进行性近视患者采用消毒灭菌的异体巩膜行后巩膜加固术,观察患者术前及术后3个月屈光度、最佳矫正视力、眼轴、视网膜动脉前期充盈时间及黄斑区视网膜厚度与形态的变化。结果 50例（92眼）患者术后屈光度、最佳矫正视力及眼轴长度均要好于术前,差异有统计学意义（P〈0.01）。术前视网膜动脉前期的充盈时间为（15.02±0.05）s,术后为（14.43±0.54）s,差异有统计学意义（P〈0.05）。40眼合并有黄斑变性患者黄斑区视网膜厚度治疗前后差异无统计学意义（P〉0.05）。本组无一例出现巩膜穿孔及血管破裂出血并发症,5例术后出现球结膜水肿,3～6个月后消失。结论后巩膜加固术治疗进行性近视安全有效。     

玻璃体切割硅油填充术后孔源性视网膜脱离患者黄斑区视网膜厚度及视功能变化

目的探讨硅油对孔源性视网膜脱离患者黄斑区视网膜厚度及视功能的影响。方法选择2017年3月至2018年2月新乡医学院第一附属医院眼科收治的未累及黄斑区的孔源性视网膜脱离患者45例,均为单眼发病,根据治疗方法分为硅油填充组25例和气体填充组20例,2组患者均施行玻璃体切割,硅油填充组患者玻璃体腔内注入5 m L硅油,气体填充组玻璃体腔内注入5 m L无菌空气,均于术前、术后1周及术后1、3、6个月检测2组患者的最佳矫正视力(BCVA),并行光学相干断层成像检查,比较2组患者黄斑中央凹区及内环区鼻侧、颞侧、上方、下方视网膜厚度。结果 2组患者术后1周患眼BCVA与术前比较差异无统计学意义(P> 0. 05);术后1、3个月,2组患者患眼BCVA均优于术前和术后1周(P <0. 05);术后6个月,硅油填充组患者患眼BCVA差于术后1、3个月(P <0. 05),与术前和术后1周比较差异无统计学意义(P> 0. 05);术后6个月,气体填充组患者患眼BCVA优于术前和术后1周(P <0. 05),与术后1、3个月比较差异无统计学意义(P> 0. 05)。术前、术后1周及术后1、3个月,2组患者患眼BCVA比较差异均无统计学意义(P> 0. 05);术后6个月,气体填充组患者患眼BCVA优于硅油填充组(P <0. 05)。术前、术后1周及术后1、3个月,2组患者之间患眼黄斑中央凹区及内环区各区域视网膜厚度比较差异均无统计学意义(P> 0. 05);术后6个月,气体填充组患者患眼黄斑中央凹区及内环区鼻侧、颞侧、下方视网膜厚度显著大于硅油填充组(P <0. 05)。2组患者术后1周患眼黄斑中央凹区及内环区各区域视网膜厚度均显著大于术前(P <0. 05)。2组患者术后1、3个月患眼黄斑中央凹区及内环区各区域视网膜厚度均显著小于术后1周(P <0. 05),而与术前比较差异无统计学意义(P> 0. 05)。2组患者术后6个月患眼黄斑中央凹区及内环区各区域视网膜厚度均显著小于术后1周(P <0. 05);硅油填充组患者术后6个月患眼黄斑中央凹区及内环区鼻侧、颞侧、下方视网膜厚度显著小于术前及术后1、3个月(P <0. 05),气体填充组患者术后6个月患眼黄斑中央凹区及内环区各区域视网膜厚度与术前及术后1、3个月比较差异均无统计学意义(P>0. 05)。结论硅油可导致视网膜厚度降低,进而影响患者视力的恢复。     

IVA+PRP治疗增殖性糖尿病视网膜病变的疗效及对视网膜各层次厚度、视力的影响

目的 针对增殖性糖尿病视网膜病变者应用玻璃体腔注射阿柏西普(intravitreous injection of arbacicept, IVA)、全视网膜光凝(panretinal photocoagulation, PRP)的疗效及对视网膜各层次厚度、视力的影响。方法 方便选取2021年9月—2022年9月泰州市中医院收治的96例增殖性糖尿病视网膜病变患者作为研究对象,以随机数表法分为观察组(48例,IVA+PRP治疗)与对照组(48例,PRP治疗),比较两组视网膜各层次厚度、中心黄斑厚度、最佳矫正视力、血清指标、治疗效果、不良事件发生率。结果 术前及术后30 d,两组黄斑区视网膜神经纤维层、神经节细胞层、内丛状层厚度比较,差异无统计学意义(P>0.05)。术前,两组中心黄斑厚度、最佳矫正视力比较,差异无统计学意义(P>0.05);术后30 d,观察组中心黄斑厚度、最佳矫正视力均低于对照组,差异有统计学意义(P<0.05)。术前,两组血清同型半胱氨酸、血清视黄醇结合蛋白4水平比较,差异无统计学意义(P>0.05);术后30 d,观察组血清同型半胱氨酸、血清视...     

儿童先天性白内障术后黄斑区视网膜厚度与脉络膜厚度的变化

目的:研究儿童先天性白内障术后黄斑区视网膜厚度变化,并分析与术后黄斑中心凹下脉络膜厚度的相关性。方法:回顾性分析42例在本院行先天性白内障手术患儿的临床资料,按年龄分成三组:3～6岁21眼,7～10岁23眼,11～18岁18眼。对术后黄斑区视网膜厚度(MRT)、黄斑中心凹下脉络膜厚度(SFCT)等相关指标进行测量分析。结果:三组患儿术后MRT分别为(239.29±35.54)、(254.83±26.42)、(219.50±16.53)μm,均高于术前,差异均有统计学意义(P0.05)。术后MRT与SFCT呈正相关(P0.05)。单眼患儿与双眼患儿的术后MRT比较,差异无统计学意义(P0.05)。单眼患儿与双眼患儿术后SFCT比较,差异无统计学意义(P0.05)。结论:儿童先天性白内障术后黄斑区视网膜厚度较术前增厚,与黄斑中心凹下脉络膜厚度呈正相关,单眼患儿和双眼患儿者术后黄斑区视网膜厚度无差异。     

糖尿病性白内障患者滤蓝光人工晶体植入术临床观察

目的：探讨糖尿病性白内障患者植入滤蓝光人工晶体治疗效果,为患者白内障术后提供更舒适的选择。方法收集糖尿病白内障患者100例（100只眼）,随机分组,50例植入普通可折叠人工晶体、50例植入滤蓝光人工晶体,术后观察最佳矫正视力、并发症,对主观视觉质量问卷调查。结果两组术后1周、1个月最佳矫正视力差异无统计学意义（ P ＞0.05）。两组患者术后早期并发症差异无统计学意义（ P ＞0.05）。两组术后1周视功能调查表指标分数比较差异有统计学意义（ P ＜0.05）。结论血糖控制在一定水平以内,滤蓝光人工晶体可以为糖尿病白内障患者提供更好选择。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.