多维元素片(21)抗运动性疲劳作用研究

目的研究多维元素片(21)抗疲劳作用。方法将昆明种雄性小鼠40只随机分为空白对照组,多维元素片(21)低(0.21 g.kg-1)、中(0.42 g.kg-1)、高(0.84 g.kg-1)剂量组,各10只。空白对照组给予等体积,纯化水。连续灌胃给药7 d,观察多维元素片(21)对小鼠负重游泳时间、肝糖原和血乳酸的影响。结果与空白对照组比较,多维元素片(21)各剂量组小鼠负重游泳时间延长,血乳酸值降低,肝糖原含量升高,以中剂量组最明显。多维元素片(21)低剂量组小鼠血乳酸值降低,小鼠肝糖原含量增加显著(P<0.05);多维元素片(21)中剂量组小鼠肝糖原含量增加显著(P<0.05),小鼠游泳时间有效延长和小鼠血乳酸值降低(P<0.01);多维元素片(21)高剂量组小鼠游泳时间延长,小鼠血乳酸值降低作用显著(P<0.05),小鼠肝糖原含量有效增加(P<0.01)。结论多维元素片(21)具有抗疲劳作用。     

人参糖肽降血糖机制(英文)

目的:研究人参糖肽(GGP)降血糖机制。方法:为了观察GGP对胰岛素分泌和糖酵解的影响,给予GGP后,测定血糖(BG)和肝糖元(LG)含量,及胰岛素、乳酸脱氢酶(LDH)、乳酸(LC)水平。通过测定给予GGP前后肝组织柠檬酸合成酶(CTS)、苹果酸脱氢酶(MDH)、琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)活性变化,推测对糖有氧氧化过程的影响。通过测定肝组织腺苷酸环化酶(AC)活性和cAMP水平,研究人参糖肽对糖代谢的影响及与第二信使cAMP的关系。通过检测肝组织磷酸化酶(PP)活性推测药物对肝糖元代谢的影响。检测药物对细胞膜受体的影响,观察了β-受体阻滞剂普萘洛尔和α-受体阻滞剂酚妥拉明对GGP作用的影响。为了证明GGP与β-受体的结合,观察了GGP与[~3H]DHA对北京鸭红细胞膜β-受体的竞争性结合。结果:GGP在降低血糖和肝糖原的剂量下(100,200mg/kg,ip或50,100mg/kg,iv),仅于给药后20分钟,小鼠血浆胰岛素水平即升高,且GGP降低血糖作用可持续16小时;同时,血中LC含量及血和肝组织LDH活性降低。而肝组织AC、CTS、MDH、SDH、COO、PP活力和cAMP水平升高,β-受体阻滞剂普萘洛尔可阻滞GP降低肝糖元作用,GGP可竞争性抑制放射配基[~3H]DHA与红细胞膜β-受体的结合,其IC_(50)为63nmol·L~(-1)。结论:GGP为β-受体激动剂,通过第二信使cAM     

淫羊藿消渴冲剂降糖作用的研究

目的考察淫羊藿消渴冲剂 (epimediumhypoglycemicspring ,EHS)对实验动物的降糖作用 ,并探讨其可能的机制。方法EHS连续灌胃给药 ,考察对正常小鼠、肾上腺素高血糖小鼠、四氧嘧啶高血糖大鼠空腹血糖 (fastingbloodglucose ,FBG)影响 ,此外还考察了EHS连续给药对四氧嘧啶大鼠肝糖原及胰岛病理组织形态学的影响。结果EHS对正常小鼠的FBG无影响 ,但可降低肾上腺素高血糖小鼠及四氧嘧啶高血糖大鼠的FBG。对其作用机制探讨发现 :EHS高、中剂量 (10 0、5 0mg·kg-1)能增加四氧嘧啶高血糖大鼠肝糖原的含量 ,光镜下观察EHS高剂量 (10 0mg·kg-1)对胰岛 β细胞有明显的修复作用。 结论EHS能降低肾上腺素高血糖小鼠和四氧嘧啶高血糖大鼠的FBG ,其机制是增加肝糖元含量及保护胰岛 β细胞     

冬虫夏草菌丝体的抗疲劳作用研究

目的研究冬虫夏草菌丝体的抗疲劳作用。方法采用雄性ICR小鼠每天灌胃给予冬虫夏草菌丝体,剂量分别为125,250,750 mg/kg,30 d后测定负重游泳时间、运动后血乳酸、肝糖原和尿素氮含量。结果冬虫夏草菌丝体能显著延长小鼠负重游泳时间(P<0.05),增加小鼠肝糖原储备量(P<0.01),降低运动后小鼠血乳酸含量(P<0.01)。结论冬虫夏草菌丝体具有抗疲劳作用。     

缢蛏水解物的抗疲劳作用

目的研究缢蛏水解物(HSC)的抗疲劳作用及机制。方法采用不同浓度HSC溶液灌胃给药,30 d后测定小鼠负重游泳时间及血清尿素氮含量、血浆乳酸浓度、血清乳酸脱氢酶活性、血红蛋白浓度、肝糖原与肌糖原含量等生化指标。结果 HSC可明显延长小鼠负重游泳时间,显著降低运动后小鼠的血清尿素氮含量和血乳酸水平,提高了血红蛋白浓度、肝糖原与肌糖原水平及乳酸脱氢酶的活性。结论 HSC具有良好的抗疲劳作用。     

枳苓六妙口服液抗疲劳作用的实验研究

目的:观察枳苓六妙口服液对小鼠的抗疲劳作用.方法:采用高、中、低不同剂量枳苓六妙口服液给正常小鼠连续灌胃30 d,测定其小鼠力竭游泳时间;运动小鼠血中血红蛋白、乳酸、尿素氮、肝糖原含量及乳酸脱氢酶活性.结果:与对照组比较,枳苓六妙口服液高、中剂量组均能明显延长小鼠的游泳时间,同时能明显提高小鼠血中血红蛋白和降低尿素氮含量(P<0.05或0.01);高、中剂量组均能显著降低血中乳酸含量和显著提高乳酸脱氢酶活性(P<0.01);低剂量、中剂量和高剂量组能明显提高肝糖原含量(P<0.05或0.01).结论:枳苓六妙口服液具有显著的抗疲劳作用,其中360 mg·kg-1效果最佳.     

珍珠粉抗疲劳作用的实验研究

目的:探讨珍珠粉对小鼠的抗疲劳作用.方法:经灌胃给予小鼠珍珠粉0.125,0.250,0.500 g·kg-1·d-1(分别相当于人体推荐摄入量的5,10和20倍),分别观察小鼠的体重、负重游泳时间、血清尿素含量、肝糖原含量和血乳酸含量.结果:珍珠粉对实验小鼠体重未见影响;在0.500g·kg-1·d-1时能延长小鼠负重游泳时间和降低运动后小鼠血清尿素含量;在0.250,0.500 g·kg-1·d-1时能增加小鼠肝糖原;且无降低运动小鼠血乳酸的作用.结论:珍珠粉具有增强小鼠抗疲劳的作用.     

垂盆草提取物对耐力训练大鼠血糖、肌糖原、肝糖原及血尿素氮的影响

目的探讨垂盆草提取物对耐力训练大鼠血糖、肌糖原、肝糖原及血尿素氮的影响,为垂盆草作为运动补剂提供理论依据。方法通过建立大强度耐力训练大鼠模型,测定运动大鼠血糖、肌糖原、肝糖原及血尿素氮影响。结果垂盆草力竭组大鼠血糖水平较运动力竭对照组升高25.62%,并有显著性差异(P<0.05)。垂盆草力竭组大鼠肌糖原含量较运动力竭对照组升高27.27%,亦有显著性差异(P<0.05)。垂盆草力竭组大鼠肝糖原含量较运动力竭对照组有显著升高(58.19%)(P<0.05)。垂盆草力竭组大鼠BUN含量较运动力竭对照组有显著下降(7.6%)(P<0.05)。结论垂盆草可以增加运动训练大鼠体内糖贮备,保证中枢神经系统、骨骼肌及红细胞等组织的能量供给,降低运动训练大鼠体内蛋白质分解代谢速率,保持肌力,延缓运动性疲劳的发生,从而提高运动能力。     

小檗碱对正常小鼠血糖调节的影响

本文探讨了小檗碱降低正常小鼠血糖的作用机制。实验表明,小檗碱未影响胰岛素的分泌与释放,也未影响肝细胞膜胰岛素受体的数目与亲和力,小檗碱的作用可能为受体后效应。实验观察到小檗碱可以对抗注射葡萄糖引起的血糖升高,可以抑制以丙氨酸为底物的糖原异生,以及小檗碱的降血糖作用与血乳酸的升高密切相关,因而小檗碱可能通过抑制糖原异生和/或促进糖酵解产生降血糖作用。     

氨杞精口服液对小鼠耐缺氧及抗疲劳作用的实验研究

目的:观察氨杞精口服液对小鼠耐缺氧能力和抗疲劳能力的影响。方法:将小鼠随机分为3组,分别为空白对照组、氨杞精口服液低剂量组、高剂量组,连续灌胃给药30 d后,测定小鼠的缺氧存活时间、负重游泳存活时间、血清尿素氮、血乳酸、肝糖原和肌糖原含量等指标的变化。结果:氨杞精口服液可以明显延长小鼠缺氧存活时间,并延长小鼠负重游泳持续时间,降低血乳酸、血清尿素氮含量,提高肝糖原含量和肌糖原含量。结论:氨杞精口服液具有提高小鼠耐缺氧及抗疲劳的作用。     

The effect of fourteen day treatment with diuretics on mouse blood sugar and glucose tolerance

Treatment of mice for 14 days with ethacrynic acid or frusemide (100 mg/kg daily orally) increased the fasting blood sugar and liver glycogen. Oral glucose tolerance was altered so that blood sugar concentrations in drug-treated groups were significantly above control values. The failure of the drugs to affect intravenous glucose tolerance and sensitivity to insulin or to tolbutamide suggested that the decrease in the oral glucose tolerance was attributable to some effect of the drug treatments on the rate of absorption of glucose from the gastrointestinal tract. Treatment with hydrochlorothiazide (100 mg/kg daily orally) exerted no effect on blood sugar, liver glycogen or glucose tolerance.     

Effect of subchronic exposure to malathion on glycogen phosphorylase and hexokinase activities in rat liver using native PAGE

The aim of this study was the evaluation of the effects of a subchronic exposure to malathion, an organophosphorus (OP) insecticide, on plasma glucose and hepatic enzymes of glycogenolysis and glycolysis in rats in vivo. Malathion was administered intragastrically by stomach tube in the amount of 1 ml corn oil containing 100mg/kg body weight (BW) daily for 32 days. At the end of the experiment, the liver was removed. The activities of glycogen phosphorylase (GP) and hexokinase (HK) were analysed in the homogenate. The methodology employed was a non-denaturing electrophoresis followed by activity-staining (native PAGE). Malathion decrease GP activity by 50% and increase HK activity by 10%. In addition, an hepatomegaly was recorded with a rise in the hepatic glycogen rate in malathion-treated rats. Moreover, subchronic administration of malathion has no effect on blood glucose concentration. The storage of glycogen in liver may be due to a stimulation of insulin secretion after the inhibition of acethylcholinesterase activity in pancreatic beta cells by malathion. These findings were in favour of an activation of glycogen storage by malathion.     

人参多肽降血糖作用

人参多肽按50,100和200mg/kg的剂量给大鼠一次ⅳ或小鼠多次sc给药,能降低正常血糖和肝糖原,但对总血脂无明显影响。同时表明,对肾上腺、四氧密啶及葡萄糖所引起的高血糖均有抑制作用,并能增强肾上腺素对肝糖原的分解。     

鬼桕酰肼自旋标记衍生物的抗氧化活性

目的:研究鬼桕酰肼自旋不达意标记衍生物的抗氧化活性及其结构活性关系.方法:用TBA比色法测自发性生成或Fe~(2 )-抗坏血酸和多柔比星刺激大鼠心、肝、肾组织匀浆生成的MDA;用分光光度法测H_2O_2诱导的大鼠RBC溶血反应;用NBT还原法测大鼠活化中性粒细胞超氧阴离于生成.结果:GP1、GP10H明显抑制MDA生成,对抗H_2O_2诱导的溶血反应,而GP和GP1H作用微弱,GP1尚抑制超氧阴离子生成.结论:氮氧自由基引入GP中,使其衍生物抗氧化作用大大增强,必需活性基因为NO基和NOH基.     

Effect of tiron and its combination with nutritional supplements against vanadium intoxication in female albino rats

In the present study an attempt has been made to evaluate the effect of Tiron along with Zinc, Selenium and Vitamin E against vanadium intoxication in female albino rats. Toxicant caused significant increase in the activities of serum transaminases, serum alkaline phosphatase and lactate dehydrogenase. Significant decrease was observed in blood sugar, serum albumin and triglyceride levels whereas serum proteins, cholesterol and urea levels increased significantly during toxicity (p 相似文献   

蜂斗菜总内酯对动物高原缺氧保护作用的研究

目的 探讨蜂斗菜总内酯对动物高原缺氧的保护作用。方法 通过建立小鼠急性密闭缺氧模型、小鼠急性减压缺氧模型以及大鼠减压缺氧模型,观察蜂斗菜总内酯对实验动物存活时间及死亡率的影响,并检测动物血糖、不同脏器中糖原、三磷酸腺苷（adenosine triphosphate,ATP）、乳酸（lactic acid,LD）、乳酸脱氢酶（lactic dehydrogenase,LDH）的含量。结果 蜂斗菜总内酯能够明显延长小鼠急性密闭缺氧的存活时间,提高存活时间增加率;能显著降低小鼠急性减压缺氧的死亡率;对于减压缺氧大鼠,能显著降低血糖含量,显著增加肝脏、骨骼肌、心肌中的糖原含量以及脑、肝脏、骨骼肌、心肌中的ATP含量,并能显著降低血浆、骨骼肌和心肌中的LD含量,降低心肌中的LDH含量。结论 蜂斗菜总内酯对小鼠急性密闭缺氧、小鼠急性减压缺氧和大鼠减压缺氧均具有明显的保护作用,其抗高原缺氧作用与其降低血糖含量,提高脏器中糖原含量、ATP含量,降低脏器中LD及LDH含量有关。     

An assessment of the in vivo efficacy of the glycogen phosphorylase inhibitor GPi688 in rat models of hyperglycaemia

BACKGROUND AND PURPOSE: Studies in cultured hepatocytes demonstrate glycogen synthase (GS) activation with glycogen phosphorylase (GP) inhibitors. The current study investigated whether these phenomena occurred in vivo using a novel GP inhibitor. EXPERIMENTAL APPROACH: An allosteric GP inhibitor, GPi688, was evaluated against both glucagon-mediated hyperglycaemia and oral glucose challenge-mediated hyperglycaemia to determine the relative effects against GP and GS in vivo. KEY RESULTS: In rat primary hepatocytes, GPi688 inhibited glucagons-mediated glucose output in a concentration dependent manner. Additionally GP activity was reduced and GS activity increased seven-fold. GPi688 inhibited glucagon-mediated hyperglycaemia in both Wistar (65%) & obese Zucker (100%) rats and demonstrated a long duration of action in the Zucker rat. The in vivo efficacy in the glucagon challenge model could be predicted by the equation; % glucagon inhibition=56.9+34.3[log ([free plasma]/rat IC50)], r=0.921). GPi688 also reduced the blood glucose of obese Zucker rats after a 7 h fast by 23%. In an oral glucose tolerance test in Zucker rats, however, GPi688 was less efficacious (7% reduction) than a glycogen synthase kinase-3 (GSK-3) inhibitor (22% reduction), despite also observing activation (by 45%) of GS in vivo. CONCLUSIONS AND IMPLICATIONS: Although GP inhibition can inhibit hyperglycaemia mediated by increased glucose production, the degree of GS activation induced by allosteric GP inhibitors in vivo, although discernible, is insufficient to increase glucose disposal. The data suggests that GP inhibitors might be more effective clinically against fasting rather than prandial hyperglycaemic control.     

Effects of nialamide on brain monoamine consumption and liver glycogen content in rats chronically treated with insulin]

Male rats weighing about 50 g were fed a normal-balanced diet for 100 days during which time i.p. injections of saline solution (2 ml/kg) or insulin (4 IU/kg) and/or nialamide (30 mg/kg) were given every two days. The animals were sacrificed 16 hr after the last injection and the brains and livers were extracted for the measurement of tyramine consumption and liver glycogen deposition. It was found that the decrease of glycogen deposition and tyramine consumption induced by nialamide was inhibited by a simultaneous administration of insulin.     

绞股蓝在大鼠肝的抗脂质过氧化作用

目的：观察绞股蓝（GP）对四氯化碳（CCl4)所致肝脏脂质过氧化的干预作用。方法：将Wistar大鼠随机分为4组：A（对照组）;B（CCL4）;C（GP);D(CCL4＋CP）。结果：CCL4组的脂质过氧化物（LPO）含量明显高于对照组,谷胱甘肽过氧化物酶（GSH－Px）活性低于对照组;而同时给予GP的D组可减弱CCL4诱发的上述作用。结论：GP能减弱CCL4对肝脏的损害。     

川西獐牙菜对低张性低氧肝损伤的保护作用

高原药用植物——川西獐牙菜(Swertia mussotii Franch)注射剂及其有效成份芒果甙和总(口山)酮对模拟高原急性低氧肝损伤具有保护作用。大鼠于低压舱内模拟海拔8,000m 2小时造成急性低氧肝损伤。预先给大鼠腹腔注射川西獐牙菜注射剂6.5ml/kg,可以明显降低大鼠SGOT和肝溶酶体酸性磷酸酶的活力,同时降低肝总脂的含量。大鼠肝溶酶体体外温孵实验显示,川西獐牙菜注射剂,芒果甙和总(口山)酮有稳定溶酶体膜的作用,并直接对溶酶体酸性磷酸酶活力有抑制作用。