成人胚胎型肝横纹肌肉瘤1例

患者男 ,73岁 ,因“B超发现右肝包块 1年 ,消瘦 ,乏力2月伴肝区疼痛 5天”入院。入院体检 :查体无异常发现。腹部B超和CT平扫均提示“肝右叶见一低密度占位 ,5cm× 7cm× 10cm大小”。实验室检查 :血清转氨酶、胆红素、碱性磷酸酶、甲胎蛋白、癌胚抗原、铁蛋白正常 ,抗 -HBe( +) ,抗 -HBc( +) ,HBV -DNA( -)。入院初诊为“肝右叶原发性肝癌”。术中见肝表面轻度纤维化 ,呈灰白色索状 ,肝脏Ⅴ、Ⅵ、Ⅶ肝段间见一约 9cm× 5cm× 6cm大小的肿块 ,质硬 ,周围无卫星结节。遂行肝右叶部分切除、门静脉置管皮下化疗泵埋入术。术后剖开标本见…     

肝畸胎癌1例报告

患者,男性,79岁,主诉以右上腹闷痛2个月于1996年3月8日入院。体检:肝右肋下5cm及剑突下6cm均可触及,质硬,边缘锐利,压痛,胆囊可触及。B超示:(1)胆囊实质性占位性病变侵及肝实质(MT)。(2)胆囊结节。CT示:胆囊癌累及左肝方叶。入院后第12天在硬膜外麻醉下行剖腹探查,见腹水1000ml,胆囊肿大,10cm×6cm×6cm,其中触及实质肿物,肝脏稍肿大,肝Ⅳ段可见4cm×3cm×3cm及2cm×1.5cm×1.5cm大小的二个结节,肝Ⅴ段可见一结节,约2.5cm×2cm×2cm大小,质均硬。逐行胆囊切除+肝Ⅳ段、Ⅴ段转移灶局部切除术。     

肝内胆管囊腺瘤1例

患者男 ,33岁。无意中自扪及右上腹包块 1周而入院。查体 :右上腹剑突下有一包块 ,表面光滑 ,质地中等 ,边界清楚 ,有囊性感 ,活动度差。上消化道钡餐透视见胃体受压 ;B型超声发现左肝叶占位性病变 19cm× 18cm,囊性内见点状回声 ,壁不规则 ;CT扫描见肝左叶低密度影占位 ,肝动脉分支及门静脉分支受压移位 ,增强扫描见肿瘤内有分隔状结构 ;术中见肿瘤位于肝左内叶 ,呈囊性 ,包膜完整 ,囊液为暗褐色。游离肝脏后 ,行左半肝切除。病理诊断 :肝内胆管囊腺瘤。讨论 :肝内胆管囊腺瘤是极少见的肝脏肿瘤性疾病 ,占全部胆管来源的肝内囊肿的 4 .6 …     

联合肝胆胰十二指肠切除及血管重建治疗肝门部胆管癌、胰头癌一例

病人　男,55 岁,以反复右上腹疼痛1 个月伴进行性黄疸 1 周为主诉入院。入院查体:神志清楚,巩膜、皮肤重度黄染,无肝掌、蜘蛛痣,腹平软,无压痛、反跳痛,右上腹部可触及肿块大小6 cm×5 cm×5 cm,肝脾未及肿大。肛查:未及肿块。彩超提示肝门部胆管癌并胆囊浸润, CT提示肝门部胆管癌浸润胆囊、右肝叶。总胆红素 250 U/dl,直接胆红素 213 U/dl,间接胆红素 37U/dl。入院第 7 天在气静复合麻醉下行剖腹探查术。术中探查胰头肿块3 cm×3 cm×3 cm,质硬,浸润胆总管全段、肝门部肿块 5 cm×4 cm×4 cm,胆囊受浸润,右肝Ⅳ、Ⅴ段可触及肿块5 cm×5…     

腹腔镜肝癌切除术11例临床报告

目的　探讨腹腔镜肝癌切除手术的可行性与适应症。方法　 2 0 0 2年 7月～ 11月 ,经临床筛选病灶位于肝脏边缘、右肝表面或左半肝的病例 11例 ,HBs Ag(+ ) 7例 ,HCVAb(+ ) 2例 ,有肝硬变者 10例 ,肝功能 Child分级 A级 6例、B级 5例 ,AFP(+ ) 4例。病灶直径大小 1.8～ 6.0 cm,平均 3 .8± 1.7cm。对于肿瘤位于左半肝实质中的肿瘤 ,选行规则性左肝切除 ;对于肿瘤位于肝脏边缘或右肝表面时 ,选用肝脏局部切除术。结果　全部 11例均成功地在完全腹腔镜下完成肝切除术 ,切除范围包括局部切除 7例 ,左肝解剖性切除 4例 ,左半肝切除 1例、左外叶切除 2例、肝方叶切除 1例。全部肿瘤完整切除 ,肿瘤包膜完整 ,无破裂。手术时间 90～ 3 2 0分钟 ,平均 2 10 .9± 71.9分钟 ,出血量 5 0～ 10 0 0 ml,平均 4 4 3 .6± 3 3 0 .5 m l。切除肝体积最大 12 cm× 8cm× 6cm3、最小 3 cm× 3 cm× 2 cm。术中未出现不能控制的并发症 ,腹腔引流管放置时间 2～ 4天。术后未发生胆漏和出血等并发症 ,术后恢复顺利 ,术后平均住院 6.4天。结论　本组研究表明腹腔镜肝癌切除术是安全可行的 ,腹腔镜肝切除的适应证可适当放宽至右肝表面或左半肝的肝癌的首选的术式     

罕见的囊性A型胸腺瘤1例

病人男,56岁。体检时发现前上纵隔肿瘤10d。病人无咳嗽、咳痰、咯血、呼吸困难及胸痛等症状。查体:气管居中,胸廓无畸形,胸骨无压痛。胸部CT检查示“前上纵隔囊性肿块,大小约11cm×6cm×3cm,性质待查”。B超检查“肝、胆、胰、脾未见明显占位”。2004年12月,入院后5d在全麻下行“前上纵隔肿块切除术”,术中见肿瘤约11cm×6cm×3cm大小,呈囊性,肿瘤与左纵隔胸膜有轻度粘连,分离胸膜,完整切除肿瘤,切除标本送病理检查。病理检查:囊性肿物,11cm×6cm×3cm大小,表面灰白、灰黄、灰褐色,切面可见3个大小不等的囊,分别为5.5cm×3.2cm、4.5cm×3.…     

原发肝脏癌肉瘤二例

例1，男，54岁，因右上腹痛半月，加重2d于2005年4月16日入院。入院查体：皮肤、巩膜无黄染，腹胀，腹肌紧张，肝大，右肋下约4指，质硬，有压痛。既往有乙肝病史8年。实验室检查：AFP101μg/L，CEA4．8μg/L，乙肝三系示：HBsAg（＋），HBeAg（＋），HBcAb（＋）。B超示：①肝硬化；②肝右叶实质性占位，肝癌可能。CT示肝脏呈小结节性硬化改变，肝右后叶7cm×6cm占位，平扫呈低密度，增强扫描无明显强化，考虑肝癌。术中探查见肝右叶Ⅴ、Ⅵ段可及7cm×6cm大小，质中偏硬肿块，膨胀性生长，边界清，尚未突破肝包膜，余肝未及明显肿块。行肝右叶Ⅴ、Ⅵ段切除术。     

带瘤生存8年原发性肝细胞癌1例

患儿 ,男 ,13岁 ,因右上腹巨大肿块八年入我院。曾求诊于多家医院 ,诊断为“肝血管瘤”、“先天性胆管扩张症”、“肝癌”等 ,无自觉症状 ,未接受治疗。体检见 :发育正常、营养中等 ,无黄疸、右上腹明显隆起 ,无腹壁静脉怒张 ,右肋缘下 15 cm触及肿块下缘 ,表面光滑、质韧、无触痛、无腹水征、肝肾功能化验正常 ,HBs Ag及 AFP正常 ,肝脏彩超示 :肝右叶探及 2 0 cm×16 cm× 10 cm回声杂乱包块、包膜完整。肝脏 CT及 MRI示 :肝右前叶巨大实质性占位病变 ,临床考虑为肝脏肿瘤行手术探查 ,术中见肿瘤来源于肝右前叶 ,约 2 0 cm× 15 cm× …     

原发性肝透明细胞癌五例诊治分析

我院自 1996年 1月至 2 0 0 2年 1月共收治原发性肝癌患者 6 0 1例 ,其中原发性肝透明细胞癌 (primaryclearcellhepa tocellularcarcinoma) 5例 ,占 0 83% (5 / 6 0 1)。报告如下。临床资料例 1　男 ,6 4岁。因右上腹胀痛两月余入院。查体无殊。B型超声提示肝硬化伴肝内占位 ,胆囊息肉。CT提示肝癌。患者有乙型肝炎史近 30年。甲胎球蛋白 (AFP) :72 8ng/ml。肝功能ChildB级。手术发现肝脏第Ⅳ段有 8cm×12cm大小肿块 ,第Ⅴ段有一结节 ,直径 1cm。遂行左肝内叶部分切除、肝Ⅴ段结节切除和胆囊切除。病理学检查示肝细胞性肝癌 (透明细胞…     

巨大胆总管扩张症1例报告

患者，女性，20岁。因上腹痛1年，症状加重伴黄疸、恶心、呕吐3天于1998年2月5日入院。查体：皮肤巩膜轻度黄疸，上腹膨隆，剑突及右肋缘下至脐下3cm可触及巨大囊性肿物，表面光滑，边界清，压痛。B超示肝左叶及右后叶内多个不规则液性肿块，CT示肝右叶、左叶及方叶多个低密度肿块影，最大肿块内16cm×11cm×21cm。术前诊断：肝多发性囊性占位病变。手术探查：肝脏面与十二指肠环部之间为一巨大囊性肿块占据，囊壁菲薄，囊肿上段为扩张的肝管及左右肝管。术中诊断：胆总管扩张症。囊内共吸出胆汁样液2300ml，检查囊壁粘膜光滑，无结石。行…     

杭州健康女性定量骨超声测定原发性骨质疏松

目的 评价杭州健康女性骨超声速度(SOS)值随增龄减少和骨质疏松患病率，建立杭州地区女性骨超声速度值参考数据库。方法 定量超声法测定1208例杭州地区健康女性桡骨远端(RAD)，第3指骨近节(PLX)，第V跖骨(MTR)和胫骨中段(TIB)的超声速度值。结果 RAD、PLX、MTR和TIBSOS峰值(Peak of SOS)均出现在40-45岁，TJB的SOS峰值出现在35—40岁，此后随年龄增长而下降。绝经后妇女在绝经后早期和晚期各有1个SOS快速减少期，前见于桡骨近端，平均年减少率为2．4％，后见于胫骨中段，平均年减少率为1．8％。各部位骨SOS累积减少率随年龄增长而增加，到85岁4部位累积减少为13％-18％。60岁以后骨质疏松性症(OP)检出率为45％-70％，OP检出率以桡骨远端最高，60-70岁平均为67％，第3指骨近端次之约50％，胫骨中段最低为36％；75岁以后分别为70％，65％和45％。结论 全身各部位骨超声速度值到达峰值的年龄不同，峰值也各有差异。绝经后妇女骨超声速度值随年龄增加减少较快，应予激素和补钙治疗，桡骨远端为本地区SOS检测和OP检出的敏感部位。     

Importance of echocardiography in prognosis of surgical outcomes in cholecystitis in elderly patients

The authors propose to use more often echocardiography (EchoCG) in examination of elderly (over 60 years) of age patients with cholecystitis that permits to increase surgical activity to 92.4%. Left ventricular ejection fraction is the most informative. When this fraction is lower than 45% surgery must be recommended on vital indications only. EchoCG was used in 155 patients with cholecystitis, 131 of them were operated. 2 (1.52%) patients died due to acute cardio-vascular insufficiency and pulmonary artery thromboembolism.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

目的 评价脊髓胶质细胞在小鼠骨癌痛形成中的作用.方法 健康雄性C3H/He小鼠40只,周龄8～10周,体重18～22 g,随机分为4组(n=10):假手术组(S组)、骨癌痛组(B组)、PBS组(P组)和米诺环素组(M组).S组跟骨骨髓腔内注射PBS 10 μl;余3组跟骨骨髓腔内注射含2×105个骨纤维肉瘤细胞的PBS 10 μl制备骨癌痛模型,于造模前即刻开始PBS组鞘内注射PBS 5μl,M组鞘内注射米诺环素(用PBS溶解为0.2 mmol/L)5μl,1次/d,连续11 d.于造模前1 d、造模后即刻、3、5、7、9、11 d时测定机械痛阈;于造模后3、7、9、11 d机械痛阈测定结束后测定冷痛阈.痛阈测定结束后处死小鼠,取脊髓组织,测定神经胶质纤维酸性蛋白(GFAP)和CD11b的表达水平.结果 与S组比较,B组和P组造模后3-11 d时、M组造模后3、5 d时机械痛阈升高,B组、P组和M组造模后7～11 d时冷痛阈升高,脊髓CD11b和GFAP表达上调(P<0.05).与B组比较,M组造模后3-11 d时机械痛阈降低,造模后7-11 d时冷痛阈降低,脊髓CD11b和GFAP表达下调(P<0.05).结论 脊髓胶质细胞(星形胶质细胞和小胶质细胞)的激活参与了小鼠骨癌痛的形成.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.