鬼针草提取物对Hela细胞及A549细胞增殖及细胞周期的影响

目的 研究鬼针草提取物(BPE)对Hela细胞及A549细胞体外增殖的抑制作用及对细胞周期的影响.方法 常规MTT法分析BPE对Hela细胞及A549细胞体外增殖的抑制作用并计算IC50;Hochest33342染色分析10 μg/mL BPE对肿瘤细胞的凋亡诱导作用;流式细胞术分析BPE对肿瘤细胞周期的影响.结果 BPE能抑制Hela细胞及A549细胞的体外增殖并呈剂量依赖性,IC50分别为10.34 μg/mL及8.31 μg/mL;Hochest染色证实BPE能诱导肿瘤细胞凋亡;流式细胞术分析证实BPE能将肿瘤细胞阻滞在G0/G1期.结论 鬼针草能使细胞停滞于G0/G1期,并能诱导肿瘤细胞凋亡从而抑制肿瘤细胞的增殖.     

松生拟层孔菌子实体乙醇提取物体外抑瘤作用初探

目的 观察松生拟层孔菌子实体乙醇提取物体外抗肿瘤活性.方法 体外药效试验采用Alamar Blue法及MTT法,观察子实体乙醇提取物对人肺癌细胞株NCI-H460、小鼠肝癌细胞株H22及小鼠肉瘤细胞株S180的增殖抑制作用.结果 其子实体乙醇提取物乙酸乙酯相在100μg/mL时,体外对NCI-H460、S180及H22...     

西瑞香素对人肝癌HepG2细胞增殖、凋亡及细胞周期的影响

目的 探讨西瑞香素对人肝癌HepG2细胞增殖、凋亡及细胞周期的影响.方法 用不同浓度的西瑞香素作用于体外培养的HepG2细胞,采用四甲基偶氮唑蓝法（MTT）检测西瑞香素对HepG2细胞增殖抑制能力;采用AnnexinV/PI双染流式细胞术测定西瑞香素对HepG2细胞凋亡的影响;采用PI单染流式细胞术检测西瑞香素对HepG2细胞周期的影响.结果 西瑞香素可抑制HepG2细胞的增殖,且存在明显的浓度和时间依赖关系;流式细胞术检测显示西瑞香素作用48 h后,实验组细胞未出现明显的早期凋亡细胞群.但实验组处于S期的细胞数较对照组明显减少（P＜0.05）,G0/G1期的细胞较对照组明显增多（P＜0.05）.结论 西瑞香素对人肝癌HepG2细胞的体外增殖具有抑制作用,可能与阻滞细胞周期有关.     

茶多酚诱导人肺癌细胞H460凋亡及对细胞周期的影响

目的 通过体外细胞培养实验探讨茶多酚对人肺癌细胞H460生长抑制作用及其作用机制.方法 体外培养人肺癌H460细胞,取对数生长期细胞,调整细胞浓度至1×105·mL-1,接种至培养板继续培养24 h,分对照组及茶多酚药物组(茶多酚终浓度为50、100、150、200、250 μg/mL),加药后继续培养12、24、36 h,应用MTT法、流式细胞仪及荧光显微镜观察茶多酚诱导细胞凋亡的作用及阻滞细胞周期的作用.结果 MTT法显示茶多酚可抑制肺癌细胞的增殖活性,其抑制作用随时间的延长而增强,以36 h抑制作用最强.在浓度50～150 μg/mL范围内,抑制作用随浓度增高而增强;当浓度大于150 μg/mL时,抑制作用随浓度增高反而减弱.流式细胞术发现茶多酚可使肺癌细胞阻滞于G1期,以36 h、150 μg/mL时阻滞作用最强(P＜0.05).荧光显微镜下观察到细胞核仁解体,核固缩,形成小突起并碎裂成多个小体等细胞凋亡的形态学改变.结论 茶多酚可诱导人肺癌H460细胞的凋亡,并使细胞周期阻滞于G1期.     

瑞香狼毒水提取物对人膀胱癌T24细胞Survivin表达的影响

目的研究瑞香狼毒水提取物对人膀胱癌T24细胞的抑制作用及生存素(Survivin)表达的影响。方法用不同浓度的瑞香狼毒水提取物处理T24细胞,分别用MTT法检测膀胱癌细胞抑制率,流式细胞术检测细胞凋亡率,免疫细胞化学检测药物作用后细胞中Survivin蛋白的表达。结果瑞香狼毒水提取物干预细胞后,①膀胱癌T24细胞增殖受到抑制,抑制效应呈剂量依赖性并随时间的延长逐渐升高;②瑞香狼毒水提取物可以促进膀胱癌T24细胞的凋亡;③膀胱癌T24细胞中的Survivin蛋白表达下降。结论瑞香狼毒水提取物可以通过下调Survivin蛋白的表达从而抑制膀胱癌T24细胞增殖,促进其凋亡,并具有浓度和时间依赖性。     

芝麻素对肝癌H22细胞增殖及H22荷瘤小鼠肿瘤生长的影响

目的观察芝麻提取物芝麻素对小鼠肝癌H22细胞增殖的影响及其对H22荷瘤小鼠肿瘤生长的抑制作用。方法用MTT法检测不同质量浓度的芝麻素对小鼠肝癌H22细胞体外增殖的影响;同时将移植H22瘤株的昆明种小鼠分成:模型组(生理盐水)、环磷酰胺(30 mg/kg)组、芝麻素高、低剂量(150、15 mg/kg)组、观察芝麻素对荷瘤小鼠的抑瘤作用。结果芝麻素在体外对H22肝癌细胞的增殖有显著的抑制作用,以8、16、32μg/mL组在48 h的抑制率最显著;芝麻素在体内对H22肿瘤细胞的生长有明显的抑制作用,芝麻素低剂量组的小鼠皮下瘤质量较模型组低(P<0.05),但仍比环磷酰胺组高(P<0.05),芝麻素高剂量组和模型组比较无显著差异(P>0.05)。结论芝麻素有明显抑制肝癌H22细胞增殖的作用。     

蒲葵子的体外抗癌活性

目的 研究蒲葵子提取物的各萃取部位体外抗肿瘤作用.方法 采用四甲基偶氮唑盐(MTT法)比色法.观察蒲葵子提取物的各萃取部位对肝癌细胞HepG2和白血病细胞HL60增殖的抑制作用.结果 蒲葵子提取物的各萃取部位在体外对肝癌细胞HepG2和白血病细胞HL60有明显的抑制作用,正丁醇部位的半数抑制率分别为7.94和10.72,效果最为显著.结论 蒲葵子提取物的各萃取部位能抑制肝癌细胞HtepG2和白血病细胞HL60的增殖,有较强的体外抗肿瘤活性.     

雷公藤内酯醇对肝癌H22细胞增殖凋亡及COX-2表达的影响

目的 观察雷公藤内酯醇(TPL)对肝癌H22细胞增殖、凋亡及环氧合酶-2(COX-2)表达水平的影响. 方法 以体外培养的鼠源性H22细胞株为研究对象,实验分对照组和不同浓度的TPL处理组.MTT比色法测定TPL对H22细胞株增殖抑制情况;TPL处理48 h后,Annexin V-FITC/PI双标记流式细胞术检测细胞凋亡情况,流式细胞术间接免疫荧光双标记法检测COX-2表达变化情况. 结果 随着药物浓度的增加及时间的延长,TPL能明显抑制H22细胞增殖.TPL诱导细胞凋亡及下调COX-2的表达具有浓度依赖性,不同浓度组比较,差别具有统计学意义(P<0.05). 结论 TPL可能通过诱导肝癌H22细胞凋亡及抑制COX-2表达发挥抗肿瘤作用.     

瑞香狼毒总黄酮提取物对肺癌细胞增殖及细胞周期的影响

目的 研究瑞香狼毒总黄酮提取物对肺癌A549细胞的增殖抑制、细胞周期的影响.方法 采用超声波技术提取瑞香狼毒根中的总黄酮.四甲基偶氮唑盐法检测总黄酮提取物对A549细胞的增殖抑制作用,流式细胞仪检测总黄酮提取物对A549细胞周期和凋亡的影响.结果 瑞香狼毒总黄酮提取物呈明显的时间-剂量依赖性抑制A549细胞的增殖,即随着时间的延长、总黄酮提取物浓度的增加,抑制作用增强;总黄酮提取物可诱导A549细胞凋亡,并将A549细胞阻滞在G1期.结论 瑞香狼毒总黄酮提取物具有抗肺癌的活性.     

迷迭香醇提取物对肺腺癌细胞增殖的影响及其机制

目的 研究迷迭香醇提取物 (RE) 对人肺腺癌细胞株 (AGZY) 增殖的影响及其机制.方法 采用改良MTT法、集落形成试验检测 RE 对 AGZY 生长增殖的影响;采用流式细胞术检测 RE 对 AGZY 细胞增殖周期的影响.结果 RE 为12.5、25、50 μg/mL和100μg/mL浓度作用5 d对AGZY的抑制率分别为47%、35%、86%和98%,半数抑制浓度IC50为17.8μg/mL;RE为25μg/mL作用AGZY 15 d,细胞集落形成明显减少,与溶媒对照比较差异有高度显著性;RE在50 μg/mL浓度作用细胞5 d,流式细胞仪检测到凋亡峰,100μg/mL有明显的凋亡峰出现.结论 RE体外明显抑制人肺腺癌细胞株(AGZY)的生长增殖,其财细胞增殖周期的阻滞可能是其抑制细胞增殖的机制之一.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.