Distribution of in situ forces in the anterior cruciate ligament in response to rotatory loads.

The anterior cruciate ligament (ACL) can be anatomically divided into anteromedial (AM) and posterolateral (PL) bundles. Current ACL reconstruction techniques focus primarily on reproducing the AM bundle, but are insufficient in response to rotatory loads. The objective of this study was to determine the distribution of in situ force between the two bundles when the knee is subjected to anterior tibial and rotatory loads. Ten cadaveric knees (50+/-10 years) were tested using a robotic/universal force-moment sensor (UFS) testing system. Two external loading conditions were applied: a 134 N anterior tibial load at full knee extension and 15 degrees, 30 degrees, 60 degrees, and 90 degrees of flexion and a combined rotatory load of 10 Nm valgus and 5 Nm internal tibial torque at 15 degrees and 30 degrees of flexion. The resulting 6 degrees of freedom kinematics of the knee and the in situ forces in the ACL and its two bundles were determined. Under an anterior tibial load, the in situ force in the PL bundle was the highest at full extension (67+/-30 N) and decreased with increasing flexion. The in situ force in the AM bundle was lower than in the PL bundle at full extension, but increased with increasing flexion, reaching a maximum (90+/-17 N) at 60 degrees of flexion and then decreasing at 90 degrees. Under a combined rotatory load, the in situ force of the PL bundle was higher at 15 degrees (21+/-11 N) and lower at 30 degrees of flexion (14+/-6 N). The in situ force in the AM bundle was similar at 15 degrees and 30 degrees of knee flexion (30+/-15 vs. 35+/-16 N, respectively). Comparing these two external loading conditions demonstrated the importance of the PL bundle, especially when the knee is near full extension. These findings provide a better understanding of the function of the two bundles of the ACL and could serve as a basis for future considerations of surgical reconstruction in the replacement of the ACL.     

Effect of axial tibial torque direction on ACL relative strain and strain rate in an in vitro simulated pivot landing

Anterior cruciate ligament (ACL) injuries most frequently occur under the large loads associated with a unipedal jump landing involving a cutting or pivoting maneuver. We tested the hypotheses that internal tibial torque would increase the anteromedial (AM) bundle ACL relative strain and strain rate more than would the corresponding external tibial torque under the large impulsive loads associated with such landing maneuvers. Twelve cadaveric female knees [mean (SD) age: 65.0 (10.5) years] were tested. Pretensioned quadriceps, hamstring, and gastrocnemius muscle‐tendon unit forces maintained an initial knee flexion angle of 15°. A compound impulsive test load (compression, flexion moment, and internal or external tibial torque) was applied to the distal tibia while recording the 3D knee loads and tibofemoral kinematics. AM‐ACL relative strain was measured using a 3 mm DVRT. In this repeated measures experiment, the Wilcoxon signed‐rank test was used to test the null hypotheses with p < 0.05 considered significant. The mean (±SD) peak AM‐ACL relative strains were 5.4 ± 3.7% and 3.1 ± 2.8% under internal and external tibial torque, respectively. The corresponding mean (± SD) peak AM‐ACL strain rates reached 254.4 ± 160.1%/s and 179.4 ± 109.9%/s, respectively. The hypotheses were supported in that the normalized mean peak AM‐ACL relative strain and strain rate were 70 and 42% greater under internal than under external tibial torque, respectively (p = 0.023, p = 0.041). We conclude that internal tibial torque is a potent stressor of the ACL because it induces a considerably (70%) larger peak strain in the AM‐ACL than does a corresponding external tibial torque. © 2011 Orthopaedic Research Society. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:528–534, 2012     

Partial Rupture of the Anterior Cruciate Ligament

The anterior cruciate ligament (ACL) consists of two major fiber bundles, namely the anteromedial (AM) and posterolateral (PL) bundles. Although disagreement exists among arthroscopic surgeons about the occurrence of isolated ruptures of the AM or PL bundle, there are reports of partial ruptures of the ACL in the literature. A potential reason for controversy could be that with conventional magnetic resonance imaging, isolated PL ruptures are difficult to diagnose because of the oblique course of this bundle. Another reason could be that isolated ruptures of the AM or PL bundle are difficult to diagnose during arthroscopy. During arthroscopy, an isolated PL bundle rupture can easily be missed when viewing from the standard anterolateral portal. The AM bundle overlies the PL bundle, and the PL bundle can only be seen by retraction of the AM bundle with a probe. When the knee is extended, the PL bundle is tight and the AM bundle is moderately lax. As the knee is flexed, the femoral attachment of the ACL becomes horizontally oriented, causing the AM bundle to tighten and the PL bundle to relax. Whereas the AM bundle is the primary restraint against anterior tibial translation in flexion, the PL bundle tends to stabilize the knee near full extension, particularly against rotatory loads. The different bundle contributions to knee stability in the flexed or extended positions can aid in the diagnosis of partial ACL ruptures. Isolated rupture of the AM bundle has more effect on the anterior drawer sign than on the Lachman test, whereas the converse is true for isolated rupture of the PL bundle. Rotational instability as a result of PL bundle rupture can be tested with the pivot-shift test. Pivot shift can be negative in cases with isolated AM bundle rupture. If only one bundle of the ACL is torn, isolated AM or PL bundle reconstruction should be considered. Although potentially difficult, a careful diagnostic evaluation is necessary before ACL surgery.     

Biomechanical characteristics of the anatomic rectangular tunnel anterior cruciate ligament reconstruction with a bone-patellar tendon-bone graft

Purpose

To clarify 1) the force sharing between two portions of BTB graft in anatomic rectangular tunnel (ART) reconstruction and 2) the knee stability in ART technique under anterior tibial load.

In vivo anterior cruciate ligament elongation in response to axial tibial loads

Background  The knowledge of in vivo anterior cruciate ligament (ACL) deformation is fundamental for understanding ACL injury mechanisms and for improving surgical reconstruction of the injured ACL. This study investigated the relative elongation of the ACL when the knee is subject to no load (<10 N) and then to full body weight (axial tibial load) at various flexion angles using a combined dual fluoroscopic and magnetic resonance imaging (MRI) technique. Methods  Nine healthy subjects were scanned with MRI and imaged when one knee was subject to no load and then to full body weight using a dual fluoroscopic system (0°–45° flexion angles). The ACL was analyzed using three models: a single central bundle; an anteromedial and posterolateral (double functional) bundle; and multiple (eight) surface fiber bundles. Results  The anteromedial bundle had a peak relative elongation of 4.4% ± 3.4% at 30° and that of the posterolateral bundle was 5.9% ± 3.4% at 15°. The ACL surface fiber bundles at the posterior portion of the ACL were shorter in length than those at the anterior portion. However, the peak relative elongation of one posterolateral fiber bundle reached more than 13% whereas one anteromedial fiber bundle reached a peak relative elongation of only about 3% at 30° of flexion by increasing the axial tibial load from no load to full body weight. Conclusions  The data quantitatively demonstrated that under external loading the ACL experiences nonhomogeneous elongation, with the posterior fiber bundles stretching more than the anterior fiber bundles.     

Teaching of anterior cruciate ligament function in osteopathic medical education

The anterior cruciate ligament (ACL) of the knee and the function of its anteromedial (AM) and posterolateral (PL) bundles are a focus of orthopedic research. Because of the probability that third-year and fourth-year osteopathic medical students will encounter ACL injuries during clinical rotations, it is of paramount importance that students fully understand the functions of the AM and PL bundles as 2 distinct functional components of the ACL. The authors assess the degree to which the AM and PL bundles are discussed within basic science curricula at colleges of osteopathic medicine (COMs). In September 2008, a 6-question survey addressing various aspects of ACL education was mailed to instructors of lower-extremity anatomy at all 28 COMs that existed at that time. Nine of the 21 responding institutions (42.9%) indicated that both the AM and PL bundles of the ACL are discussed within their basic science curricula. Four of these 9 COMs indicated that their instruction mentions that the bundles are parallel in extension and crossed in flexion. Nine of the 21 responding COMs (42.9%) indicated that they instruct students that the AM bundle is a major anterior-posterior restrictor, and 12 (57.1%) indicated that they instruct students that the PL bundle is the major rotational stabilizer of the ACL. In 7 of the 21 responding COMs (33.3%), the AM and PL bundles are identified via direct visualization during anatomic dissection of the ACL. The authors conclude that their findings suggest the need for enhanced presentation of the AM and PL bundles within the basic science curricula at COMs to provide osteopathic medical students with a more comprehensive education in anatomy.     

Double-bundle anterior cruciate ligament reconstruction with split Achilles allograft and single tibia tunnel for small ACL tibial footprint : technical note with clinical results

Purpose

We describe a surgical technique of double-bundle ACL reconstruction with a single tibia tunnel and report the clinical outcome.

Methods

The Achilles tendon portion was split longitudinally into two separate bundles, namely, an anteromedial (AM) bundle with 7–8 mm diameter and a posterolateral (PL) bundle with 4–6 mm diameter. The central portion of the calcaneal bone plug was prepared with a diameter of 10 mm and a length of 30 mm. For the femoral tunnel preparation, we preferred inside out target through an accessory anteromedial portal for an approach to native ACL footprint and outside in reaming through separate incision on the lateral aspect of distal thigh to prevent cartilage injury of medial femoral condyle. 10 mm diameter of single tibia tunnel was prepared at the central portion of ACL tibial footprint. After graft passage from tibia to femoral side, fixation of calcaneal bone plug within the tibia tunnel was performed using two bioabsorbable cross pins. Then, AM bundle was first fixed at 45° of flexion while the PL bundle was fixed at 10° of flexion using bioabsorbable interference screws and augmented staples. Clinical results of 22 patients (18 males and 4 females, average age 30.7 years) who underwent double-bundle anterior cruciate ligament (ACL) reconstruction with this technique were evaluated.

Results

At an average follow-up of 30 months, there was significant improvement of the Lysholm knee score, the 2,000 IKDC subjective knee score, the median Tegner activity score and the side-to-side difference. According to the 2,000 IKDC knee examination form, the grade rated as normal in seven patients, nearly normal in 14 patients and abnormal in one patient at the latest follow-up. There were no postoperative complications and revisional surgeries.

Conclusions

Split Achilles allograft and single tibia tunnel technique for double-bundle ACL reconstruction can be an alternative option for patients with small tibial insertion sites.

Level of evidence

Level IV, therapeutic study.     

Potential risk of cartilage damage in double bundle ACL reconstruction: impact of knee flexion angle and portal location on the femoral PL bundle tunnel

The aim of this study was to compare the impact of knee flexion angle and the level of the medial drilling portal on a potential damage to the subchondral bone in double bundle ACL reconstruction, drilling the femoral PL tunnel through an accessory medial portal. We hypothesized that a knee flexion angle of 70° and 90° or a high accessory medial portal will result in a potential damage to the subchondral bone of the lateral femoral condyle. In a sawbone knee model, the medial portal location was standardized as 0 mm above the meniscus (low portal) and 10 mm above the meniscus (high portal). Femoral PL bundle tunnels were drilled at three different knee flexion angels: 70°, 90°, and 110° of knee flexion. For each portal, ten specimens were used for every flexion angle. Drilling the PL tunnel through the high medial portal at a knee flexion angle of 70° resulted in damage of the subchondral bone plate in all specimens. At 110° of flexion the distance of the tunnel exit to the subchondral bone plate was significantly higher than at 70° of flexion for both the groups, drilling through the high and low medial portal (P < 0.05). Drilling through the low portal did not result in bone plate damage at 90 and 110° of knee flexion angle. Drilling of the femoral PL bundle tunnel through a high medial portal at low knee flexion angles may damage the subchondral bone of the lateral compartment. In ACL reconstruction restoring the AM and PL bundle separately, high medial portal drilling should be avoided. We recommend drilling of the femoral PL bundle tunnel through a low medial portal in high knee flexion.     

Evaluation of bone tunnel placement for suture augmentation of an injured anterior cruciate ligament: Effects on joint stability in a goat model

Use of novel tissue engineering approaches to heal an injured anterior cruciate ligament (ACL) requires suture repair and/or augmentation to provide joint stability. We evaluated the effects of the location of suture augmentation at the femur and tibia in terms of joint stability using a goat model. Eight goat stifle joints were tested with augmentation sutures placed in two femoral tunnel locations: (1) anterior to, or (2) through the ACL footprint, and two tibial tunnel locations: (1) medial to, or (2) medial and lateral to the footprint. Using a robotic/universal force‐moment sensor testing system, the anterior tibial translation (ATT) and the corresponding in situ force carried by the sutures were obtained at 30°, 60°, and 90° of flexion in response to external loads. No significant differences were found between augmentation groups due to tunnel location in terms of ATT or the in situ forces carried by the sutures at all flexion angles tested. Similar results were found under 5 N m of varus–valgus torque. Under a 67 N anterior tibial load, the ATT was restored to within 3 mm of the intact joint following suture augmentation (p > 0.05). Suture augmentation, when placed close to the ACL insertion, could be helpful in providing initial joint stability to aid ACL healing in the goat model. Published by Wiley Periodicals, Inc. J Orthop Res 28:1373–1379, 2010     

Assessment and Augmentation of Symptomatic Anteromedial or Posterolateral Bundle Tears of the Anterior Cruciate Ligament

The anterior cruciate ligament (ACL) consists of 2 anatomic and functional bundles, the anteromedial (AM) and posterolateral (PL) bundle. Depending on the mechanism of injury, there are different injury patterns to the AM and PL bundles, demonstrating a wide spectrum of partial ACL tears. Clinical interest has recently focused on establishing pre- and intraoperative ways of assessing the different types of symptomatic partial ACL injuries in order to perform an individual ACL augmentation according to the specific injury pattern. Theoretically, sparing the intact parts of the ACL may increase vascularization and proprioception, may optimize the accuracy of the ACL reconstruction, and may result in better stability and improved clinical outcome for the patient. However, an isolated reconstruction of the AM or PL bundle is an advanced arthroscopic procedure that requires a precise pre- and intraoperative diagnostic assessment of the injury pattern, an exact arthroscopic knowledge of the anatomic insertion sites, a careful debridement, and bone tunnel placement while preserving the intact parts of the ACL. This article will present the concept of partial ACL tears and will describe the clinical, radiologic, and arthroscopic assessment and the arthroscopic technique of isolated AM or PL bundle augmentation.     

Anatomic assessment of femoral tunnel by transtibial drilling technique in double-bundle anterior cruciate liga- ment reconstruction： an in vivo study

Objective： To evaluate the anatomy of femoral tunnels created by simulated transtibial technique in double-bundle anterior cruciate ligament （ACL） reconstruction. Methods： Two tibial tunnels, anteromedial （AM） and posterolateral （PL）, were drilled 45° and 55° to tibial plateau respectively. On the femoral side, the AM and PL tunnels were drilled through anteriomedial portal. After the four tun- nels were established, the shaft of a reamer was introduced into the joint through tibial tunnel and reached against the lateral wall of intercondylar notch. The position that the reamer shaft can reach was marked and recorded. Results： Neither femoral AM nor PL tunnel opening can be fully or partially reached by the reamer shaft through the tibial AM tunnel in all cases. The evaluation through the tibial PL tunnel showed that only in 8 of 50 cases （16%） the femoral AM tunnel opening and in 4 cases （8%） the PL opening can be fully reached. On the other hand, in 12 cases （24%） the femoral AM tunnel opening and in 10 cases （20%） the PL opening can be partially reached by the shafts through the tibial PL tunnel. Conclusion： The result strongly suggests that transtibial technique is not well competent for femoral tun- nel drilling in anatomic double-bundle ACL reconstruction as we have hypothesized.     

Effect of increased quadriceps tensile stiffness on peak anterior cruciate ligament strain during a simulated pivot landing

ACL injury prevention programs often involve strengthening the knee muscles. We posit that an unrecognized benefit of such training is the associated increase in the tensile stiffness of the hypertrophied muscle. We tested the hypothesis that an increased quadriceps tensile stiffness would reduce peak anteromedial bundle (AM‐)ACL relative strain in female knees. Twelve female cadaver knees were subjected to compound impulsive two‐times body weight loads in compression, flexion, and internal tibial torque beginning at 15° flexion. Knees were equipped with modifiable custom springs to represent the nonlinear rapid stretch behavior of a normal and increased stiffness female quadriceps (i.e., 33% greater stiffness). Peak AM‐ACL relative strain was measured using an in situ transducer while muscle forces and tibiofemoral kinematics and kinetics were recorded. A 3D ADAMS? dynamic biomechanical knee model was used in silico to interpret the experimental results which were analyzed using a repeated‐measures Wilcoxon test. Female knees exhibited a 16% reduction in peak AM‐ACL relative strain and 21% reduction in change in flexion when quadriceps tensile stiffness was increased by 33% (mean (SD) difference: 0.97% (0.65%), p = 0.003). We conclude that increased quadriceps tensile stiffness reduces peak ACL strain during a controlled study simulating a pivot landing. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:423–430, 2014.

     

In situ forces in the anterior cruciate ligament and its bundles in response to anterior tibial loads

The anterior cruciate ligament has a complex fiber anatomy and is not considered to be a uniform structure. Current anterior cruciate ligament reconstructions succeed in stabilizing the knee, but they neither fully restore normal knee kinematics nor reproduce normal ligament, function. To improve the outcome of the reconstruction, it may be necessary to reproduce the complex function of the intact anterior cruciate ligament in the replacement graft. We examined the in situ forces in nine human anterior cruciate ligaments as well as the force distribution between the anteromedial and posterolateral bundles of the ligament in response to applied anterioi tibial loads ranging from 22 to 110 N at knee flexion angles of 0–90°. The analysis was performed using a robotic manipulator in conjunction with a universal force-moment sensor. The in situ forces were determined with no device attached to the ligament, while the knee was permitted to move freely in response to the applied loads. We found that the in situ forces in the anterior cruciate ligament ranged from 12.8 ± 7.3 N under 22 N of anterior tibial load applied at 90° of knee flexion to 110.6 ± 14.8 N under 110 N of applied load at 15° of flexion. The magnitude of the in situ force in the posterolateral bundle was larger than that in the anteromedial bundle at knee flexion angles between 0 and 45°, reaching a maximum of 75.2 ± 18.3 N at 15° of knee flexion under an anterior tibial load of 110 N. The magnitude of the in situ force in the posterolateral bundle was significantly affected by knee flexion angle and anterior tibial load in a fashion remarkably similar to that seen in the anterior cruciate ligament. The magnitude of the in situ force in the anteromedial bundle, in contrast, remained relatively constant, not changing with flexion angle. Significant differences in the direction of the in situ force between the anteromedial bundle and the posterolateral bundle were found only at flexion angles of 0 and 60° and only under applied anterior tibial loads greater than 66 N. We have demonstrated the nonuniformity of the anterior cruciate ligament under unconstrained anterior tibial loads. Our data further suggest that in order for the anterior cruciate ligament replacement graft to reproduce the in situ forces of the normal anterior cruciate ligament, reconstruction techniques should take into account the role of the posterolateral bundle in addition to that of the anteromedial bundle.     

Stability evaluation after isolated reconstruction of anteromedial or posterolateral bundle in symptomatic partial tears of anterior cruciate ligament

Objective

To evaluate the stability achieved in isolated reconstruction of each ACL bundles (minimum 2-year follow-up).

Materials and methods

Study group: 39 consecutive patients (28.1 years of mean age) underwent anteromedial (AM) and posterolateral (PL) bundle reconstruction surgery (31.71 months of mean follow-up). Control group: 36 non-concurrent patients using single-bundle (SB) technique (more than 2-year follow-up). Evaluation based on IKDC scores, taking anterior–posterior translation and rotational stability as primary endpoints, and epidemiological data, ischaemia time, waiting time for surgery and complications as secondary endpoints.

Results

SB, AM and PL groups showed an anteroposterior translation (APT) at 2 years of 2.3, 1.8 and 1.8 mm, respectively, with an APT reduction of 5.4, 2.9 and 2.3 mm, respectively, but with no difference between both types of partial reconstruction (p = 0.552). IKDC scores in the AM group were as follows: preoperative (11 cases in group A, 12 B, 3 C), postoperative (24 A, 2 B); IKDC in the PL group: preoperative (7 B, 6 C), postoperative (10 A, 3 B). Mobility restored in all cases, while in group B, 2 AM patients and 3 PL had a slight Pivot Shift. There were no differences in complications as compared to conventional techniques.

Discussion

All techniques showed global significant enhancement in rotational stability (p < 0.0005). Improvement in anterior–posterior translation in AM group and in rotational stability in PL group was achieved; both showed no relevant statistical significance. Residual translation shows directly proportional relationship with preoperative status, surgical ischaemia time and patient weight.

Conclusion

Reconstruction of one ACL bundle or the other can restore knee stability and function. Care should be taken to detect where the PL bundle reaches maximum tension and in improving patient preoperative status and ischaemia time.     

Anatomic variations between Japanese and Caucasian populations in the healthy young adult knee joint

Our objective was to characterize variations in mechanical knee alignment, tibial torsion, tibial width, and ACL laxity measurements between Japanese and Caucasian populations in the healthy, young adult knee joint. Seventy young adult subjects participated in this study, including 23 Japanese and 47 Caucasian subjects. Coronal magnetic resonance images of the hip, knee, and ankle were acquired for analysis. Japanese subjects had a significantly higher (p = 0.04) varus alignment (1.64 ± 0.43° standard error) than Caucasians (0.55 ± 0.33°), while women exhibited a more valgus alignment (0.16 ± 0.52°) than men (0.94 ± 0.42°, p = 0.04). Significant differences were found in tibial torsion and ACL laxity (p < 0.01) between ethnicities, with Japanese exhibiting lower tibial torsion (33.4 ± 10.0°) and higher ACL laxity (7.5 ± 0.4 mm) measurements compared to Caucasians (38.9 ± 9.5° and 5.7 ± 0.3 mm, respectively). Significant differences between genders were found in hip‐knee‐ankle alignment (p = 0.04), tibial width (p < 0.0001), and ACL laxity (p < 0.01) measurements. Measurements were reliable between observers and for repeated positioning. Our study provides new insight into anatomical and geometric differences in the knee joint between Japanese and Caucasians, as well as between females and males. Further consideration of these results may improve development of implants to accommodate for these differences, and understanding of characteristics leading to increased prevalence of knee OA in certain populations. The use of magnetic resonance imaging to obtain these measurements also allows soft tissue structure characterization without exposure to ionizing radiation. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res     

Rotational instability of the knee: internal tibial rotation under a simulated pivot shift test

Introduction  Recently, several publications investigated the rotational instability of the human knee joint under pivot shift examinations and reported the internal tibial rotation as measurement for instrumented knee laxity measurements. We hypothesize that ACL deficiency leads to increased internal tibial rotation under a simulated pivot shift test. Furthermore, it was hypothesized that anatomic single bundle ACL reconstruction significantly reduces the internal tibial rotation under a simulated pivot shift test when compared to the ACL-deficient knee. Methods  In seven human cadaveric knees, the kinematics of the intact knee, ACL-deficient knee, and anatomic single bundle ACL reconstructed knee were determined in response to a 134 N anterior tibial load and a combined rotatory load of 10 N m valgus and 4 N m internal tibial rotation using a robotic/UFS testing system. Statistical analyses were performed using a two-way ANOVA test. Results  Single bundle ACL reconstruction reduced the anterior tibial translation under a simulated KT-1000 test significantly compared to the ACL-deficient knee (P < 0.05). After reconstruction, there was a statistical significant difference to the intact knee at 30° of knee flexion. Under a simulated pivot shift test, anatomic single bundle ACL reconstruction could restore the intact knee kinematics. Internal tibial rotation under a simulated pivot shift showed no significant difference in the ACL-intact, ACL-deficient and ACL-reconstructed knee. Conclusion  In conclusion, ACL deficiency does not increase the internal tibial rotation under a simulated pivot shift test. For objective measurements of the rotational instability of the knee using instrumented knee laxity devices under pivot shift mechanisms, the anterior tibial translation should be rather evaluated than the internal tibial rotation. This study was supported in part by a grant of the German Speaking Association of Arthroscopy (AGA).     

3D CT evaluation of femoral and tibial tunnels in anatomic double bundle anterior cruciate ligament reconstruction

BackgroundAn anatomical double bundle ACL reconstruction replicates the anatomy of native ACL as the tunnels are made to simulate the anatomy of ACL with AM and PL bundle foot prints. The goal of anatomic ACL reconstruction is to tailor the procedure to each patient’s anatomic, biomechanical and functional demands to provide the best possible outcome. The shift from single bundle to double bundle technique and also from transtibial to transportal method has been to provide near anatomic tunnel positions.PurposeTo determine the position of femoral and tibial tunnels prepared by double bundle ACL reconstruction using three dimensional Computed tomography.Study designA prospective case series involving forty patients with ACL tear who underwent transportal double bundle ACL reconstruction.MethodComputed tomography scans were performed on forty knees that had undergone double bundle anterior cruciate ligament reconstruction. Three-dimensional computed tomography reconstruction models of the knee joint were prepared and aligned into an anatomical coordinate axis system for femur and tibia respectively. Tibial tunnel centres were measured in the anterior-to-posterior and medial-to-lateral directions on the top view of tibial plateau and femoral tunnel centres were measured in posterior to anterior and proximal-to-distal directions with anatomic coordinate axis method. These measurements were compared with published reference data.ResultsAnalysing the Femoral tunnel, the mean posterior-to-anterior distances for anteromedial and posterolateral tunnel centre position were 46.8% ± 7.4% and 34.5% ± 5.0% of the posterior-to-anterior height of the medial wall and the mean proximal-to-distal distances for the anteromedial and posterolateral tunnel centre position were 24.1% ± 7.1% and 61.6% ± 4.8%. On the tibial side, the mean anterior-to-posterior distances for the anteromedial and posterolateral tunnel centre position were 28.8% ± 4.3% and 46.2% ± 3.6% of the anterior-to posterior depth of the tibia measured from the anterior border and the mean medial-to-lateral distances for the anteromedial and posterolateral tunnel centre position were 46.5% ± 2.9% and 50.6% ± 2.8% of the medial-to-lateral width of the tibia measured from the medial border. There is high Inter-observer and Intra-observer reliability (Intra-class correlation coefficient).Discussion and conclusionFemoral AM tunnel was positioned significantly anterior and nearly proximal whereas the femoral PL tunnel was positioned significantly anterior and nearly distal with respect to the anatomic site. Location of tibial AM tunnel was nearly posterior and nearly medial whereas the location of tibial PL tunnel was very similar to the anatomic site Evaluation of location of tunnels through the anatomic co-ordinate axes method on 3D CT models is a reliable and reproducible method. This method would help the surgeons to aim for anatomic placement of the tunnels. It also shows that there is scope for improvement of femoral tunnel in double bundle ACL reconstruction through transportal technique.