sPD-1和sPD-Ls在结缔组织病发病机制中的作用

膜结合型程序性死亡受体1(membrane-bound programmed cell death-1,mPD-1)及膜结合型程序性死亡受体配体(membrane-bound programmed cell death-ligands,mPD-Ls)存在可溶性形式,分别为可溶性程序性死亡受体1(soluble programmed cell death-1,sPD-1)和可溶性程序性死亡受体配体(soluble programmed cell death-ligands,s PD-Ls)[包括可溶性程序性死亡受体配体1(soluble programmed cell death-ligand 1,sPD-L1)和可溶性程序性死亡受体配体2(soluble programmed cell death-ligand 2,sPD-L2)]。s PD-1和sPD-L2主要由PD-1 mRNA的选择性剪接异构体翻译产生,而sPD-L1则是由基质金属蛋白酶(matrix metalloproteinases,MMPs)剪切膜结合型程序性死亡受体配体1(membrane-bound programm...     

PARP-1与PD-1、PD-L1在子宫内膜癌组织中的表达及临床意义

目的 探讨多聚ADP核糖聚合酶-1(poly(ADP-ribose)polymerase-1,PARP-1)与程序性死亡蛋白(programmed cell death protein-1,PD-1)、程序性死亡蛋白配体-1(programmed cell death protein ligand-1,PD-L1)在子...     

冠心病患者CD8、PD-1、PD-L1、TGF-β1、MMP-14血浆含量与冠状动脉狭窄程度的相关性分析及预测价值

目的 借助生物信息学分析和临床样本检测,探究人血浆白细胞分化抗原(CD8)、程序性死亡受体-1(pro-grammed cell death protein-1,PD-1)、细胞程序性死亡配体-1(programmed cell death ligand-1,PD-L1)、转化生长因子-β1(transforming ...     

幼年型卵巢颗粒细胞瘤伴破裂出血1例及文献回顾

卵巢颗粒细胞瘤(ovarian granulosa cell tumor,OGCT)是具有粒层细胞形态特征的性索间质肿瘤,仅占卵巢恶性肿瘤的1％ ～2％.分为成人型颗粒细胞瘤(adult granulosa cell tumor,AGCT)和幼年型颗粒细胞瘤(juvenile granulosa cell tumor,...     

司美替尼下调KRAS G12V突变型非小细胞肺癌细胞PD-L1水平的探索性研究

目的·探究不同Kirsten大鼠肉瘤病毒癌基因(Kirsten rat sarcoma viral oncogene,KRAS)突变型非小细胞肺癌(non-small cell lung cancer,NSCLC)患者肿瘤组织及细胞系与程序性死亡蛋白配体1(programmed cell death ligand 1,...     

PD-1/PD-L1抑制剂在小细胞肺癌治疗中的进展

小细胞肺癌(small cell lung cancer,SCLC)具有高度侵袭性、致死性,对化疗和放疗敏感但易复发,总体生存率较差。目前对于广泛期(extensive-stage disease,ED)小细胞肺癌(SCLC)免疫治疗的临床研究获得较好的疗效,给小细胞肺癌的治疗带来新的希望。其中包括以程序性死亡受体-1(programmed cell death-1,PD-1)/程序性死亡配体-1(programmed cell death-ligand 1,PD-L1)抑制剂为主的免疫检查点抑制剂单药及多药联合的临床研究。本文旨在阐述PD-1/PD-L1抑制剂在SCLC治疗中的研究进展。     

Treg细胞亚型在首诊癌症患者外周血中的表达及意义

目的 探讨癌症患者外周血中调节性T细胞(regulatory T cells, Tregs)亚型即1型辅助性T细胞(type 1 helper T cell, Th1)样Treg细胞(T-bet⁺Treg)、Th2样Treg细胞(GATA3⁺Treg)、Th9样Treg细胞(Pu-1⁺Treg)、Th17样Treg细胞(RORγ⁺Treg)及滤泡型辅助性T细胞(follicular helper T cell, Tfh)样Treg细胞(CXCR5⁺Treg)的表达情况及其对应的CD4⁺T细胞子集：Th1、2型辅助性T细胞(type 2 helper T cell, Th2)、9型辅助性T细胞(type 9 helper T cell, Th9)、17型辅助性T细胞(type 17 helper T cell, Th17)、Tfh的表达。方法 将23例癌症患者设为研究组，23例门诊健康体检者设为正常对照组，采用流式细胞术检测患者外周血中T-bet^+...     

Changes of T cell subsets in peripheral blood of patients with Schistosomiasis japonica and their relation to interleukin-1.

T cell subsets in peripheral blood were phenotyped in 56 patients with different stages of Schistosomiasis japonica, including 17 with acute, 14 with chronic and 25 with advanced infection. The activity of interleukin-1 (IL-1) produced by peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) in vitro was simultaneously detected in these three groups of patients. It was found that the percentages of CD3+ (total T cell), CD4+ (helper/inducer T cell) and CD8+ (suppressor/cytotoxic T cell) T cell and the level of IL-1 were significantly increased in the group of acute Schistosomiasis japonica. In the groups of chronic and advanced Schistosomiasis japonica, the proportion of CD3+ T cell, the ratio of CD3+/CD4+ and the level of IL-1 were remarkably reduced, and the percentage of CD8+ T cell was increased. The rate of CD4+ T cell was obviously decreased in cases patients with advanced Schistosomiasis japonica. The percentage of CD4+ T cell was positively correlated to the level of IL-1 in the three groups of patients. These results indicate that T cell subsets and IL-1 may play an important role in the immunoregulation of Schistosomiasis japonica.

     

脐血间质干细胞的基础研究和应用进展

干细胞(Stem Cell)也称前体细胞(precursorcell)或祖细胞(progenitor cell),是机体内存在的一类原始幼稚型特殊细胞,其显著的生物学特性是既具有自我更新的能力又具有多向分化的潜能[1-2].根据发育阶段的不同,干细胞可分为胚胎干细胞(embryonic stem cell,ESC)和成体干细胞(adult stem cell).     

免疫检查点抑制剂相关性肝损伤的研究进展

<正>免疫检查点抑制剂(Immune checkpoint inhibitors,ICI)是一种新型的抗肿瘤免疫治疗药物,它以程序性细胞死亡蛋白-1(Programmed cell death protein-1,PD-1)及其配体-1(Programmed cell death-1ligand 1,PD-L1)和细胞毒性T淋巴细胞相关抗原-4(Cytotoxic T lymphocyte antigen-4,CTLA-4)等作为作用靶点,阻止T细胞表面受体与其配体相结合,     

Synthesis,characterization and cytotoxicity of platinum(II) complexes with 2-methyl-2-substituted phenoxypropanoic acid as leaving ligands

A class of novel platinum(II) complexes with 2-methyl-2-substituted phenoxypropanoic acids as leaving ligands were designed and synthesized.All complexes were characterized by IR,1H NMR and ESI-MS spectra.The in vitro antiproliferative activities were tested by MTT assay against three human cancer cell lines,indicating that the complexes showed selective cytotoxicity to human gastric cancer cell lines (SGC-7901) with only weak antiproliferative activities were observed against human hepatocellular carcinoma cell line (HepG2) and human non-small cell lung cancer cell line (A549).     

Peroxisome Proliferator-Activated Receptor-γActivated by Ligands Can Inhibit Human Lung Cancer Cell Growth through Induction of Apoptosis

To study the expression of peroxisome proliferator-activated receptor-γ(PPAR-γ) in lungcancer cells, and to testify if the PPAR-γ agonists can inhibit human lung cancer cell growth throughinduction of apoptosis, PPAR-γ was detected in two lung cancer cell lines by RT-PCR and immuno-histochemistry, the inhibition of human lung cancer cell growth was investigated by MTT and cellcounts, and the apoptosis was assessed by TUNEL. The results showed that: (1) PPAR-γ expressedon two lung cancer cell lines; (2) PPAR-γ activated by ligands could inhibit human lung cancer cellgrowth remarkably; (3) PPAR-γ agonists could induce apoptosis to inhibit lung cancer cell growth.It was concluded that PPAR-γ expressed in lung cancer cell can be activated by ligands and can inhib-it lung cancer cell growth through induction of apoptosis.     

Primary nasopharyngeal non-Hodgkin lymphoma and its relationship with Epstein-Barr virus infection

Objectives To investigate the immunophenotypes of primary nasopharyngeal non-Hodgkin lymphoma(NPL) and their relationship to Epstein-Barr virus (EBV) infection.Methods The clinical data and biopsies of 73 patients with NPL were collected in Guangzhou. In situ hybridization was performed to detect the EBV-encoded small non-polyadenylated nuclear RNAs(EBERs) on biopsy slides. Immunohistochemistry was used to classify the immunophenotypes of NPL and detect EBV antigen expression.Results Forty-four (60. 27%) of the 73 NPLs were of B cell lineage (CD79α^ /CD3^-/CD56^-)while the 29 others (39.73%) were of non-B cell lineage. Seventy-three NPLs could be classified into 3 major immunophenotypes: B cell (CD79α^ /CD3^-/CD56 ^-, 44 cases), peripheral T cell (CD79α^-/CD3^ /CD56^-, 22) and NK/T cell (CD79α^-/CD3^ /CD56^ , 7). The percentages of EBV infection differed among the 3 major immunophenotypes ( B cell : 11.36%, 5/44 ; peripheral T cell: 81.82%,18/22; NK/T cell: 100%, 7/7). Both CD56-positive and CD56-negative immunophenotypes could further be divided into 4 subtypes: CD8^-/CD4^-, CD8^ /CD4^- , CD8^-/CD4^ and CD8^ /CD4^ All the CD8^-/CD4^- NPLs with CD56-positivity (7) or CD56-negativity (2) were infected with EBV. The neoplastic cells of a nasopharyngeal Burkitt‘ s lymphoma expressed EBV nuclear antigen 1 (EBNA1)and EBV RNA (EBERs) only. In the other 29 EBV-infected NPLs, most of the lymphoma cells harboring EBV also expressed EBNA1 and EBERs; 21 of the 29 NPLs had a considerable number of neoplastic cells expressing latent membrane protein 1 ( LMP1 ) (21/29, 72.41% ) and 23 of 29 NPLs expressed latent membrane protein 2A (LMP2A) (23/29, 79. 31%). A few lymphoma cells in 17( 17/29, 58. 62% ), 23 (23/29, 79.31% ) and 22 NPLs (22/29, 75.86% ) expressed Zta ( Barn HI Z transactivator), viral capsid antigen (VCA) and membrane antigen (MA), respectively.Conclusions The prevalence ratio of the 3 immunophenotypes, namely, B cell, peripheral T cell and NK/T cell lymphoma, is about 6:3: 1. However, the EBV infection ratio is reversed, 1:8: 10. All the NK/T cell (CD56^ ) and peripheral immature T cell (CD3^ /CD8^-/CD4^-) NPLs were EBV-infected.Except for one Burkitt‘ s lymphoma, the EBV harbored in both B cell and non-B cell NPLs was mainly latent infection, type It, expressing EBNA1, LMP1 and LMP2A. However, the EBV found in a few lymphoma cells could become replicative, expressing lytic proteins.     

Glomerular disease

970367 Upregulation of cell adhesion molecules byendothelin-1 on human mesangial cells in vitro. LINJingmei(林京梅), et al. Nephrol Div, 1st Hosp, BeijingMed Univ, Beijing, 100034. Chin J Nephrol 1997; 13(2): 67-69 Objective: To study the expression of intercellularadhesion molecule-1(ICAM-1)and vascular cell adhe-     

抗PD-1/PD-L1单抗药物致甲状腺不良反应的研究进展

程序性细胞死亡受体1(programmed cell death-1,PD-1)和程序性细胞死亡受体配体1(programmed cell death ligand-1,PD-L1)在肿瘤分期、复发、转移、浸润及预后中发挥着重要的作用,抗PD-1/PD-L1单抗药物在多种肿瘤的治疗中取得了令人鼓舞的进展,但是在治疗的同...     

原发性晚期直肠小细胞癌1例报告

<正>小细胞癌(Small cell carcinoma,SCC)绝大部分发生于肺内,即小细胞肺癌(Small cell lung cancer,SCLC);仅有2.5%~4%发生于肺外器官,即肺外小细胞癌(Extrapulmonary small cell carcinoma,ESCC)。ESCC发生常见部位有所差异,总体上食管、子宫颈和咽喉部较常见[1]。笔者现对1例原发性晚期直肠小细胞癌的治疗进行分析,现报告如下。     

The Biological Research of Clinical Behavior of Laryngeal Cancer

The present study analyzed the DNA content(ratio of heterploid,RH),s-phase cell(SPC)per-centage,the proliferating cell nuclear antigen(PC-NA) labelling index and area of nucleolar organizerregion (AgNOR) in 57 patients with laryngeal can-cer. The results were shown in Table 1.     

非小细胞肺癌PD-1/PD-L1免疫检查点治疗疗效预测标志物

程序化死亡受体-1(programmed cell death-1, PD-1)/程序化死亡受体的配体(programmed cell death-ligand 1, PD-L1)免疫检查点抑制剂治疗非小细胞肺癌(non-small cell lung cancer, NSCLC)成为免疫治疗研究的热点。临床实践中发现,并非所有患者均能从免疫治疗中获益,因此,研究者正在试图寻找合适的疗效预测标志物来筛选可受益患者。目前关于非小细胞肺癌PD-1/PD-L1免疫检查点治疗疗效预测标志物研究主要包括以下方面:PD-L1表达、肿瘤突变负荷(tumor mutation burden,TMB)、肿瘤浸润淋巴细胞(tumor infiltrating lymphocytes,TILs)及免疫炎症因子、基因突变。     

幼年型卵巢颗粒细胞瘤1例报告

卵巢颗粒细胞瘤(granulosa cell tumor of the ovary,GCT)属于低度恶性的性索间质肿瘤,占卵巢肿瘤的5％.按其临床和病理特点分为成人型颗粒细胞瘤(adult granulosa cell tumor of the ovary,AGCT)和幼年型颗粒细胞瘤(juvenile granulosa cell tumor,JGCT).现将本院收治的1例JGCT患者临床资料、治疗方法和愈后情况报道如下.