藏药牛尾独活药材薄层色谱鉴别方法研究

目的：探索牛尾独活使用用薄层色谱的鉴别方法。方法：采用硅胶G薄层板,以经鉴定的牛尾独活为对照品,用石油醚-甲苯-醋酸乙酯（8：2：2）为展开剂,在254nm观察荧光下显色。结果：供试品与对照品在相应的位置上显相同的斑点。     

不同产地黄芩药材的鉴别

目的通过药材来源性状、薄层色谱及紫外线色谱的差别对不同产地黄芩药材进行鉴别。方法对黄芩、滇黄芩、甘肃黄芩进行性状鉴别,然后选择黄芩、滇黄芩、甘肃黄芩分别制作供试品及黄芩苷、黄芩素、汉黄芩素对照品进行薄层色谱分析,紫外线色谱分析,对3种不同产地黄芩进行鉴别。结果 3种黄芩在来源性状、薄层色谱、紫外线色谱上存在异同点,在结合多种鉴别方法后可初步判断。结论联合传统方法和先进科技手段有助于黄芩鉴别,可为临床药材选择提供科学依据,提高中药制剂质量。     

益津降糖胶囊中主要成分的定性实验研究

目的制定益津降糖胶囊的质量标准,控制并提高药品质量,为保证临床用药安全,规范控制质量提供依据。方法分别取样品（益津降糖胶囊）、人参、仙人掌、甘草对照药材及其原料药材,阴性对照药材制成供试品溶液、对照药材溶液、原料药材溶液、阴性对照样品溶液,用薄层色谱法对益津降糖胶囊中人参、仙人掌、甘草成分进行定性鉴别。结果益津降糖胶囊中人参、甘草供试品色谱中,在与对照药材相应位置上,显示相同颜色的斑点;益津降糖胶囊中仙人掌的供试品色谱与对照药材色谱在相同位置处未见有相同斑点。结论建立了益津降糖胶囊中人参、甘草的定性鉴定方法,该方法简便、快速、重现性和专属性好。仙人掌的鉴别方法有待进一步研究。     

冠脉丸的薄层色谱鉴别

目的建立冠脉丸的薄层鉴别方法。方法采用TLC法对处方中人参和丹参进行定性鉴别。结果制剂中人参和丹参的薄层色谱斑点清晰,阴性对照无干扰。结论所建立的鉴别方法简便,专属性强,重现性良好,可用于冠脉丸的定性质量控制。     

肾宁胶囊的制备及质量控制

目的:研究肾宁胶囊的制备及质量控制方法.方法:确定制备工艺,对部分药材进行薄层色谱鉴别.结果:制备工艺可靠,建立了对处方中主药黄芪、丹参、熟地、山茱萸的薄层色谱鉴别方法.结论:鉴别方法快速,重现性好,稳定可靠,可作为该制剂质量控制的定性指标.     

瑶药风湿骨痛喷雾剂的质量标准研究

目的建立瑶药风湿骨痛喷雾剂的质量控制方法。方法采用薄层色谱法分别对复方中断肠草、两面针进行定性鉴别;采用高效液相色谱法对君药断肠草中的钩吻素子进行含量测定。结果薄层色谱中,供试品溶液在与对照品相应位置上出现相同颜色的斑点,阴性无相应的斑点;高效液相色谱中,钩吻素子在0.1992～3.1872μg范围内呈良好的线性关系,r=0.9997,平均加样回收率为100.4%,RSD=1.90%。结论本法操作简单,重现性好,结果准确,可作为该制剂的质量控制方法。     

泌淋胶囊的薄层色谱鉴别研究

目的:探讨泌淋胶囊中头花蓼与石椒草的薄层色谱鉴别方法。方法:采用薄层色谱(TLC)法。结果:薄层色谱鉴别斑点清晰,分离度好,阴性对照无干扰。结论:该方法简单可行,结果准确可靠、重现性好,可作为泌淋胶囊的质量参考标准。     

仙珍骨宝胶囊中蛇床子素的薄层色谱鉴别方法的条件摸索

目的:建立仙珍骨宝胶囊中的蛇床子素的薄层层析定性鉴别方法。方法:采用薄层层析法对仙珍骨宝胶囊中蛇床子进行定性鉴别。结果:供试品的色谱中,在与对照品、对照药材相应的位置上,均显相同颜色的斑点。结论:该方法斑点清晰,重现性好,可作为仙珍骨宝胶囊的定性控制方法。     

薄层色谱法鉴别紫地合剂及四种止血方剂中的地稔

目的建立紫地合剂中地稔药材的薄层色谱鉴别方法,制定紫地合剂的质量标准之一——鉴别项。方法采用薄层色谱法对紫地合剂中的主要成分地稔进行鉴别,并以同是止血经典方的十灰散、小蓟饮子、咳血方、黄土汤四个方剂为辅助鉴别,检验方法的普遍性。结果本文所建立的方法稳定、可靠,无干扰,可作为该制剂的质量控制方法之一。该方法对于鉴别复方制剂中的地稔具有普遍意义。     

当归益血膏的薄层鉴别

目的：通过对当归益血膏中有效成分的鉴别,为其质量控制提供依据。方法：采用薄层色谱法,对当归、川芎进行鉴别。结果：供试品色谱中,在与当归、川芎对照药材色谱相应的位置上,显相同颜色的荧光斑点。结论：该法操作简便,重现性好,可用于当归益血膏质量控制。     

出国体检人员中可疑氯胺酮阳性病例确证分析

目的建立一种快速、灵敏的方法用于氯胺酮初筛为阳性后的确证。方法采用传统的液-液萃取法进行人体尿液样品前处理,并结合单四极杆气相色谱质谱联用法(GC/MS)对样品进行确证分析。结果该氯胺酮初筛阳性尿样在确证分析中未检出氯胺酮。被检尿样的GC/MS谱图中未见明显的氯胺酮色谱峰;而经质谱定性后发现谱图中有较高的麻黄素色谱峰。结论本研究建立的GC/MS分析方法对于毒品确证十分准确、有效,可推广应用于其它毒品初筛为阳性的进一步确证实验中。     

对羟基苯基卟啉金属配合物的光谱与色谱特性研究

目的 研究卟啉与金属离子形成配合物的光谱、色谱特性。方法 采用扫描配合物的吸收光谱和液相色谱分离的方法。结果 卟啉与4种金属离子形成配合物的最大吸收波长、配合物的稳定性及在HPLC上的分离特性不同。结论 金属离子的离子半径、核外电子排布、卟啉显色剂的空腔大小、流动相组成、色谱柱等都是影响配合物光谱、色谱性质的重要因素。     

Porcine Inhibin: Initial Fractionation as a High Molecular Weight Complex

A high molecular weight complex or aggregate of inhibin was obtained by chromatography of porcine follicular fluid in Fractogel TSK65F. Recovery of activity was good (usually 80-100%), but only 30-60% was recovered as a high molecular weight complex (greater than 160,000) free of albumin and gamma globulin (the two major proteins in follicular fluid). The balance of the activity was distributed in the gamma globulin-albumin region of the chromatogram (i.e., 160,000 down to 65,000 daltons). Distribution in this region of the chromatogram in part reflected the prior processing of the sample (e.g., it was augmented by ethanol or acetone precipitation prior to chromatography). The utility of Fractogel chromatography lies in its ability to resolve a large portion of the inhibin activity from the major proteins (albumin and gamma globulin), plus an efficient recovery of activity.

Maximum purification on the Fractogel chromatograms was approximately 20-fold. This product and the other Fractogel fractions were tested for protease activity by a sensitive slab gel procedure. All fractions contained detectable protease activity that could potentially affect inhibin activity during further fractionations. This was shown with a protease fraction isolated from porcine follicular fluid by affinity chromatography. When added to a partially purified inhibin preparation, this protease fraction destroyed 77% of the inhibin activity.     

现代化大型综合性医院筹建模式初探

介绍了现代化大型综合性医院——深圳市滨海医院的项目概况。通过对比以往大型综合性医院采取成立临时性筹建组的筹建模式，重点介绍了滨海医院项目成立筹备办公室负责医院筹建工作的筹建模式和具体做法，旨在探讨适合现代化大型综合性医院又好又快的建设筹建工作模式。     

Effect of preparation methods on the assessment of airborne concentrations of asbestos fibres by transmission electron microscopy

The usual indirect and direct preparation methods used to assess the airborne asbestos fibre concentrations by transmission electron microscopy (TEM) have been investigated in detail. It has been shown that ultrasonics, often used in the indirect preparation method to facilitate the recovery of dust, may significantly increase the fibre number concentration. This effect is highly variable and dependent on the characteristics of the workplace where the samples were collected. If the indirect preparation method is modified to avoid the use of the ultrasonic treatment, both the direct and indirect preparation methods give comparable results for fibres of length greater than 5 μm. By contrast, the number concentrations of fibres shorter than 5 μm are always different but the reason for this is not clear.     

四种口服清肠溶液在肠镜检查中的临床应用与分析

目的分析四种不同的口服溶液在肠镜检查中的应用,找到一种患者较舒适且肠腔清洁满意度高的肠道准备方法。方法回顾性分析2009年8月~2010年12月我院1500例肠镜检查患者口服不同的清肠溶液清肠的临床资料,观察其肠道清洁率及患者舒适度。结果口服复方聚乙二醇电解质溶液组患者肠道清洁率为93%,不良反应发生率为15.9%,与其他三组比较,差异有显著性（P〈0.05）。结论口服复方聚乙二醇电解质溶液患者较为舒适,并可获得满意的肠道清洁率,是临床病人镜检肠道准备的理想方法之一。     

气相色谱法测定卫生杀虫剂的有效含量

目的：测定混用杀虫剂的有效成分；方法：利用气相色谱氢火焰检测器同时测定卫生杀虫剂中的益必添、胺菊酯、溴氰菊酯、氯氰菊酯、氯菊酯等有效成分；结果：用3．5％OV-17填充柱为色谱柱，回收率在84．78％-108．21％之间；结论：方法简便、准确。     

Identification of volatile components in waste waters by combined head-space gas chromatography-mass spectrometry.

A method for the identification of volatile components in effluent waters is described. The sample is heated in a closed vessel at 80 degrees C and the head-space vapour injected in a gas chromatograph coupled to a mass spectrometer. Mass spectra are recorded every 4 seconds and stored by a Data Acquisition System. The reconstructed spectra enable very easy identification of the peaks of the chromatogram. The important contribution of the computer system to the sensitivity of the technique is demonstrated.     

Two approaches to mutation detection based on functional data

A new technique, denaturing high-performance liquid chromatography (dHPLC), allows for detection of any heterozygous sequence variation in a gene without prior knowledge of the precise location of the sequence change. The results of a dHPLC analysis are recorded in real-time in the form of a chromatogram that is sequence-specific. In this paper we present methods to classify an individual, based on the observed chromatogram, as a homozygous wild-type or a carrier of a specific variant for the given DNA segment by comparison to representative chromatograms that are obtained from the training set of individuals with known variant status. The first approach consists of finding a parsimonious parametric model and then classifying each newly observed curve based on comparing the most discriminating characteristic, the main mode, to the main mode of the training curves. The second approach consists of finding empirical estimates of the modes of each chromatogram and using a bootstrap test for equality with the corresponding estimates of the training curves. We apply both methods to data on the breast cancer susceptibility gene BRCA1 and test the performance of the methods on independent samples.     

强效排石颗粒的研制及其临床评价

目的研究强效排石颗粒的制备方法,并观察其临床疗效。方法观察其临床疗效,并进行动物急性毒性试验。结果符合颗粒的质量要求。动物试验LD50>12·08g/kg生药。治疗组与对照组总有效率均在75%以上,2者均具有明显的治疗作用。结论组方合理,工艺可行,无毒性;临床疗效满意。