CO_2激光联合胸腺五肽局部注射治疗尖锐湿疣疗效观察

目的观察CO2激光联合胸腺五肽预防尖锐湿疣（CA）复发的疗效。方法选择91例CA患者随机分为两组,治疗组53例给予CO2激光治疗,并在皮损处局部注射胸腺五肽;对照组38例仅予CO2激光治疗。结果治疗后2个月、4个月、6个月治疗组复发率分别为0、5.66%、13.21%,对照组分别为15.79%、34.21%、47.37%。对照组复发率均明显高于治疗组（P〈0.05）。结论 CO2激光联合胸腺五肽预防尖锐湿状（CA）复发的效果良好。     

胸腺五肽局部注射配合CO_2激光治疗尖锐湿疣的临床观察

目的了解胸腺五肽局部注射配合CO2激光治疗尖锐湿疣的疗效。方法对64例尖锐湿疣患者在应用CO2激光治疗仪将疣体去除干净后在皮损局部皮下注射胸腺五肽。结果6个月后57例治愈,7例复发;对照组62例中,37例治愈,25例复发,两组差异有统计学意义（P〈0.1）。结论局部注射胸腺五肽能在局部发挥抗病毒、免疫调节作用,可减少该病复发。     

钬激光在男性难治性肛管尖锐湿疣治疗中的应用

目的 探讨钬激光治疗肛管尖锐湿疣的临床疗效及安全性。方法 回顾性收集2017年5月—2019年9月在上海市皮肤病医院光医学治疗科接受钬激光27例男性肛管尖锐湿疣患者作为治疗组,另外,选择同期接受CO2激光的35例男性患者作为对照组进行研究,观察肛管内疣体清除率、复发率及不良反应。结果 单次钬激光疣体清除有效率（85.2%）高于CO2激光（54.3%）,差异有统计学意义（P=0.005）;6个月随访中钬激光治疗组的疣体复发率（25.9%）明显低于CO2激光组（51.4%,P=0.042）。两组患者治疗后均有局部水肿、浅溃疡等反应,2～3周痊愈,未见感染、瘢痕、排便困难等现象。结论 钬激光治疗肛管疣体清除率高、复发率低,副作用小,值得临床推广应用。     

干扰素局部注射联合激光治疗前尿道尖锐湿疣

目的观察尿道局部注射干扰素联合CO2激光治疗男性前尿道尖锐湿疣的疗效。方法CO2激光去除疣体、尿道局部注射干扰素治疗男性前尿道尖锐湿疣患者61例。结果经观察6～12个月，26例（42．6％）经1次激光治疗痊愈，22例（36．1％）经2次激光治疗痊愈。结论尿道局部注射干扰素联合CO2激光治疗男性前尿道尖锐湿疣简便、经济、疗效确切。     

CO2激光联合甘露聚糖肽注射液治疗尖锐湿疣的疗效观察

目的 探讨CO2激光联合甘露聚糖肽注射液治疗尖锐湿疣的疗效观察.方法 将140例尖锐湿疣患者分为两组,联合治疗组72例,激光组68例,治疗组在CO2激光气化疣体后给予甘露聚糖肽注射液肌肉注射,激光组仅给予CO2激光气化疣体.结果 6个月内治疗组治愈63例（87.5％）,复发9例（12.5％）;对照组治愈45例（66.18％）,复发23例（33.82％）.联合治疗组治愈率明显高于激光组,复发率低于激光组,两组差异有统计学意义（P＜0.05）.结论 CO2激光联合甘露聚糖肽注射液肌肉注射治疗尖锐湿疣效果较佳,值得临床推广.     

CO_2激光联合白细胞介素-2局部注射治疗尖锐湿疣疗效观察

目的:探讨CO2激光联合白细胞介素-2局部注射治疗尖锐湿疣的临床疗效。方法:将96例尖锐湿疣患者随机分为治疗组50例,对照组46例。两组病例均用CO2激光去除可见疣体。治疗组治疗后隔日1次局部注射白细胞介素-2,10～15次为一个疗程;对照组在激光治疗后,不再用任何其他治疗,疗程结束后观察疗效、复发情况。结果:治疗组痊愈44例,复发6例,痊愈率为88.0%,复发率为12.0%;对照组痊愈27例,复发19例,痊愈率为58.7%,复发率为41.3%。结论:白细胞介素-2具有免疫调节和抗病毒作用,CO2激光联合白细胞介素-2局部注射治疗尖锐湿疣有较好的临床疗效,可明显降低治疗后复发率。     

改性几丁质喷雾剂治疗术后尖锐湿疣疗效观察

目的观察激光联合改性几丁质喷雾剂治疗肛周、肛管尖锐湿疣疗效,探讨减少肛周、肛管尖锐湿疣复发的治疗方法。方法将我院门诊肛周、肛管尖锐湿疣68例患者随机分成两组,治疗组34例,对照组34例,两组年龄、性别、病程、疣体发病部位、疣体大小、数量无显著差异。两组患者均应用CO2激光手术治疗,治疗组术后应用改性几丁质喷雾剂。对照组术后应用0．01％新洁尔灭溶液。结果治疗组治愈率91．18％,对照组治愈率76．47％,治疗组、对照组比较,x2=2．7113,P〈0．05。结论激光联合改性几丁质喷雾剂治疗肛周、肛管尖锐湿疣具有止痛、控制细菌和病毒感染、促进创面愈合的作用,能降低复发率,是治疗肛周、肛管尖锐湿疣较好的方法。     

卡介菌和咪喹莫特联合CO2激光对尖锐湿疣复发的疗效观察

目的 探讨卡介菌多糖核酸和咪喹莫特联合CO2激光治疗对尖锐湿疣复发的临床治疗效果.方法 将171例复发性尖锐湿疣患者随机分为4组:观察组(A组)43例和对照组1组(B组)41例,对照组2组(C组)42例,对照组3组(D组)45例.A组用CO2激光去除疣体后肌注卡介菌多糖核酸注射液,并于伤口愈合后予咪喹莫特外用,疗程为3个月.B组用CO2激光去除疣体后仅肌注卡介菌多糖核酸注射液,C组用CO2激光去除疣体并于伤口愈合后予咪喹莫特外用,疗程为3个月.D组仅使用CO2激光去除疣体.4组观察期均为6个月.结果 4组复发率分别为13.95％、31.70％、33.33％、71.11％,D组复发率最高,B组与C组复发率无明显差异,A组复发率低于其他各组.结论 卡介菌多糖核酸和咪喹莫特联合CO2激光治疗复发性尖锐湿疣可以降低复发率,值得在临床上推广.     

二氧化碳激光联合胸腺五肽和异维A酸治疗尖锐湿疣的疗效观察

目的:观察二氧化碳激光联合胸腺五肽和异维A酸治疗尖锐湿疣的临床疗效。方法:将尖锐湿疣患者120例随机分为观察组及对照组,各60例。对照组给予二氧化碳激光治疗联合胸腺五肽局部注射,观察组在此基础上加用异维A酸胶丸口服。随访6个月,比较2组患者复发率及治愈率。结果:观察组复发率(15%)显著低于对照组(46.67%),观察组治愈率(85%)显著高于对照组(53.33%)。2组均未出现影响治疗的严重不良反应。结论:对尖锐湿疣患者,在二氧化碳激光治疗基础上联合应用胸腺五肽注射液局部注射以及异维A酸胶丸口服,可显著提高治愈率,降低复发率。     

低功率氦氖激光联合CO2激光治疗尖锐湿疣临床观察

目的观察低功率氦氖激光联合CO2 激光治疗尖锐湿疣的临床疗效。方法将102例患者随机分为2组，治疗组48例给予CO2激光治疗，同时采用低功率氦氖激光照射皮损处，对照组54例仅采用CO2激光治疗，2组均随访6月。结果治疗组痊愈率81．3％，复发率为63％；对照组痊愈率75．9％，复发率26．4％，2组复发率比较，差异有统计学意义（χ2=7．32，P〈0．05）。结论CO2激光联合氦氖激光治疗尖锐湿疣疗效好，不良反应少。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.