高脂膳食大鼠血清对人肺腺癌细胞系SPCA1增殖活性的影响

目的 用血清生理学方法直接从细胞水平研究膳食脂肪对肺癌细胞增殖的影响。方法 SD雄性大鼠分为两组，分别喂以普通饲料及高脂饲料。30d时取血并分离血清，用含20％大鼠血清的：RPMll640培养液培养人肺腺癌细胞SPCA1，从MTT比色法、Giemsa染色、核增殖抗原(PCNA)表达三个方面观察两组血清对细胞增殖活性的影响。结果 MTT比色法结果显示高脂组细胞在第5天出现生长活跃的状态，使生长曲线反常上扬。Giemsa染色结果发现高脂组细胞呈局灶样增殖，在每个增殖灶的周围有多个呈花瓣状分布的细胞坏死灶。对照组中细胞染色较浅，有多个坏死灶，而无增殖灶出现。核增殖抗原检测结果发现高脂组PCNA阳性细胞数表达明显多于对照组，且阳性信号表达较强。结论 高脂膳食大鼠血清能促进人肺腺癌细胞系SPCA1增殖，其机制可能涉及多个方面。     

盐亭食管癌高发区饮食对人食管癌细胞增殖的影响

目的采用血清生理学方法,观察盐亭食管癌高发区饮食喂饲大鼠血清对人食管癌细胞Eca-109生长增殖的影响。方法将24只雄性SD大鼠分为3组,分别喂饲大鼠常规饲料、健康成人饮食及盐亭饮食7天,每天定时记录采食量及体重。用MTT法探讨用大鼠血清培养人食管癌细胞的适宜条件;分别以人正常肝上皮细胞HL7702及10%灭活小牛血清作为对照,采用细胞生长曲线、细胞群体倍增时间、3H-TDR掺入实验研究盐亭饮食喂饲大鼠血清对人食管癌细胞生长增殖的影响。结果喂饲健康成人饮食与常规饲料的大鼠其采食量及体重无差异;用5%未灭活大鼠血清取代10%灭活小牛血清适合人食管癌细胞培养;盐亭食管癌高发区饮食喂饲大鼠血清可明显促进人食管癌细胞生长,却不利于人正常肝上皮细胞生长,且与其他3个组均有统计学差异(P<0.05)。结论盐亭食管癌高发区饮食具有促进人食管癌细胞Eca-109生长增殖的作用。     

硒和锌对人食管癌细胞株Eca109生长增殖影响的血清生理学研究

目的观察不同剂量硒、锌灌胃大鼠血清对人食管癌细胞株Eca109生长增殖的影响。方法将SD大鼠随机分为7组(基础饲料组、低硒组、高硒组、低锌组、高锌组、低硒低锌组、高硒高锌组),每组8只。喂养30天后取大鼠血清培养人食管癌细胞株Eca109和人正常肝上皮细胞株HL7702。用AAS法分别测定各组大鼠血清硒、锌;采用MTT法、3H-TDR掺入实验研究不同浓度硒、锌灌胃大鼠血清对两株细胞生长增殖的影响。结果 (1)基础饲料组血清硒、锌水平最低,高锌组血清锌最高;高硒高锌灌胃组大鼠血清硒水平最高,低硒低锌灌胃组血清硒水平次之,均明显高于基础饲料组;而此两组大鼠血清锌与基础饲料喂饲组大鼠血清锌水平差异无统计学意义(P>0.05);(2)与小牛血清对照组相比,只有高硒高锌灌胃组大鼠血清从第72h起抑制癌细胞生长(P<0.05),其余各组均促进食管癌细胞的生长;且该组大鼠血清也抑制肝细胞生长(P<0.05);(3)高硒高锌灌胃组大鼠血清明显抑制食管癌细胞DNA合成(P<0.05),其余各组与对照组作用相近,但该组对肝细胞DNA合成的抑制作用也最强。结论硒、锌在吸收、代谢等方面可能存在相互抑制作用;血清硒、锌含量较低会促进人食管癌细胞的增殖,而增加硒、锌的摄入可提高血清硒、锌的含量且可能抑制癌细胞的增殖。     

大鼠血清培养液对食管癌细胞增殖影响

目的筛选食管癌细胞增殖培养液中大鼠血清的最佳浓度和活性状态。方法采用常规饲料喂饲大鼠30d,股动脉采血制备血清,采用不同浓度和活性状态的大鼠血清培养液培养人食管上皮鳞癌细胞株Eca-109,采用四甲基偶氮噻唑蓝(MTT)法,筛选大鼠血清培养液对Eca-109细胞生长增殖的最佳作用条件,以小牛血清培养液及人正常肝上皮细胞株HL7702作为对照进行比较。结果各浓度未灭活大鼠血清培养的人食管癌细胞Eca-109基本上均呈指数增长趋势,72h时各浓度组间差异有统计学意义(F=159.263,P0.05);用5%未灭活大鼠血清培养人食管癌细胞Eca-109和人正常肝上皮细胞HL7702,培养72h时,2株细胞的生长增殖能力均强于5%灭活大鼠血清,差异均有统计学意义(F=37.746,P0.05;F=43.399,P0.05);与小牛血清对2株细胞生长增殖的作用接近(P0.05)。结论 5%相同浓度条件下,未灭活大鼠血清培养液更适合细胞生长。     

谷豆复合物、谷豆复合膳食纤维及单一谷物膳食纤维对脂代谢紊乱的影响

目的观察谷豆复合物、谷豆复合膳食纤维和全谷物玉米膳食纤维(DF)对脂代谢紊乱大鼠血脂及肝脏脂肪酸合成酶(FAS)活性,及其对大鼠肝组织固醇调节元件结合蛋白-1c(SREBP-1c)mRNA表达的影响,比较谷豆复合物、谷豆复合DF与单一谷物DF改善脂毒性效果。方法 50只SD大鼠适应性喂养1周后,随机分成阴性对照组、高脂模型组、谷豆复合物组、谷豆复合DF组和玉米DF组;以相应的饲料连续喂养8周后,测定各组大鼠总胆固醇(TC)、甘油三酯(TG)、空腹血糖(FBG)、高密度脂蛋白胆固醇(HDL-C)和FAS等指标,测定各组大鼠肝脏SREBP-1c mRNA的表达。结果与阴性对照组相比,高脂模型组的大鼠血清TC、TG水平显著升高(P0.05);与高脂模型组相比,谷豆复合物组、谷豆复合DF组和玉米DF组大鼠血清TC、TG水平显著降低(P0.05);HDL-C水平显著高于高脂模型组,大鼠肝脏脂肪酸合成酶活性及SREBP-1c的表达显著降低。结论谷豆复合膳食纤维可改善脂代谢紊乱大鼠的血脂水平,降低FAS活性及SREBP-1c的表达水平。     

断乳后不同剂量铁强化膳食对大鼠成年期肥胖诱导后瘦素水平的影响

目的:研究断乳大鼠铁强化膳食对其成年高脂膳食诱导肥胖后血清瘦素水平的影响。方法:将85只初断乳雄性SD大鼠随机分为基础饲料组和高(4倍)、中(3倍)、低(2倍)剂量铁强化组。4周后各组均以基础饲料喂养1周,于第5周末测定大鼠体脂和血清瘦素含量。将基础饲料组16只大鼠随机分为正常对照组和肥胖诱导组,肥胖诱导组和3个铁强化组均喂以高脂饲料。高脂干预8周后处死所有大鼠计算脂/体比,检测血清瘦素含量。结果:高脂干预后,肥胖诱导组体重、体脂含量、瘦素水平均高于其他各组,3个铁强化组瘦素含量高于正常对照组。结论:生命早期适量补铁能够促进大鼠成年期肥胖诱导后的脂肪分解,维持大鼠体重平衡,保证机体瘦素分泌及功能正常。     

高脂饮食肥胖大鼠胰岛细胞凋亡与Mcl-1表达及意义

目的 通过建立高脂饮食诱导肥胖大鼠模型,研究肥胖大鼠胰岛细胞中线粒体途径抗凋亡蛋白髓样细胞白血病因子-1(Mcl-1)表达情况及其与β细胞凋亡的相关性,探究肥胖大鼠胰岛细胞损伤可能的机制。方法 SPF级3周龄雄性SD大鼠72只,随机分为普通饲料组和高脂饲料组 。在高脂喂养8、16、20、24周末筛选出符合肥胖标准的大鼠设为肥胖组,普通饲料组设为对照组。ELISA检测血清,游离脂肪酸(FFA)水平, 通过透射电镜观察胰岛β细胞微观结构改变,免疫组化SP法检测胰岛组织中Mcl-1蛋白的表达情况,并通过Real-Time PCR检测胰腺组织中Mcl-1的mRNA表达水平,原位末端标记法(TUNEL法)检测两组大鼠的胰岛细胞凋亡率。结果 1)高脂喂养20周,肥胖组大鼠血清FFA水平显著高于对照组(P<0.01);2)电镜结果显示,16周末肥胖组大鼠胰岛β细胞结构与对照组相比,出现凋亡样改变,且随时间推移有所加重;3)高脂喂养20周,肥胖组大鼠抗凋亡相关蛋白Mcl-1表达较对照组明显降低(P<0.05);4)RT-PCR结果显示, 高脂喂养16周,抗凋亡相关蛋白Mcl-1的mRNA水平明显降低(P<0.05);5)高脂喂养20周,与对照组大鼠相比,肥胖组凋亡率显著增加(P<0.01);6)大鼠胰岛细胞凋亡率与血清FFA水平呈正相关(r＝0.536,P<0.01),与Mcl-1基因的表达水平呈负相关(r＝-0.776,P<0.01)。结论 高脂饮食肥胖大鼠胰岛β细胞形态结构异常, 线粒体途径抗凋亡蛋白Mcl-1表达降低,胰岛细胞凋亡率与Mcl-1表达呈负相关。提示肥胖大鼠存在胰岛β细胞损伤,其机制与线粒体凋亡途径有关。     

黑加仑原汁调节血脂的实验研究

[目的]研究黑加仑原汁对高脂饲料喂养大鼠血脂的调节作用。[方法]正常wistar大鼠给予高脂饲料的同时分别饮用不同剂量的黑加仑原汁三周后,检测大鼠血清甘油三酯、高密度脂蛋白、低密度脂蛋白和胆固醇、空腹血糖、血清胰岛素、血清瘦素7项指标。[结果]干预3周后,黑加仑高剂量、中剂量、低剂量组大鼠血清甘油三酯分别为0 18±0 07 mmol/L 、0 21 ±0 08 mmol/L、0 23±0 07 mmol/L ,明显高于高脂组大鼠血清甘油三酯0 53±0 18 mmol/L(P<0 01); 高脂组大鼠血清胆固醇为7 84±1 34 mmol/L 明显高于黑加仑高剂量组大鼠血清胆固醇5 79 ±1 77mmol/L(P<0 01)。[结论]实验表明黑加仑原汁可以明显降低高脂饲料喂养大鼠血清甘油三酯水平、高剂量可以明显降低血清胆固醇水平。黑加仑原汁具有降低高脂饲料喂养大鼠血清甘油三酯作用。     

营养

072360膳食对大鼠动脉窖蛋白-1表达的影响/许明佳…∥中国公共卫生.-2006,22(5).-566～567研究膳食脂类对大鼠动脉窖蛋白-1(caveolin-1)表达的影响。雄性SD大鼠试验组用高脂饲料喂养15周诱导肥胖(DIO)和肥胖抵抗(DIO-R)后,将DIO组一半改用基础饲料喂养(DIO-HF/LF),另一半继续高     

铁皮石斛叶对二代繁殖雌性大鼠免疫水平影响的研究

目的研究铁皮石斛叶对二代繁殖大鼠免疫功能的影响。方法取按2.0 g/kg~6.4 g/kg BW铁皮石斛叶喂养二代后的雌性大鼠24只,以正常基础饲料喂养二代后雌性大鼠为对照,采用大鼠脾淋巴细胞转化能力、绵羊红细胞诱导大鼠DTH(足跖增厚法)、NK细胞杀伤活性、抗体生成细胞检测(Jerne改良玻片法)、腹腔巨噬细胞吞噬鸡红细胞能力、血清溶血素试验,测定铁皮石斛叶对二代繁殖雌性大鼠免疫水平的影响。结果高剂量组大鼠NK细胞活性高于对照组(P0.05)。与阴性对照组比较,3个剂量组的吞噬率、吞噬指数差异均无统计学意义(P0.05);3个剂量组大鼠的血清溶血素抗体积数值、溶血空斑数与阴性对照组间差异均无统计学意义(P0.05);淋巴细胞增殖能力实验光密度差值、左后足跖厚度差异均有统计学意义(P0.05);3个剂量组胸腺/体重比值、脾脏/体重比值差异均无统计学意义(P0.05)。结论在本实验条件下,铁皮石斛叶可一定程度提高二代繁殖雌性大鼠的免疫水平。     

Effect of palatable hyperlipidic diet on lipid metabolism of sedentary and exercised rats

OBJECTIVE: The present study was designed to examine 1) whether continuous feeding with a palatable hyperlipidic diet and cycling this diet with chow diet would affect lipid and carbohydrate metabolism in a similar way; and 2) whether the effect of chronic exercise on lipid and carbohydrate metabolism would be modified by these diet regimens. METHODS: Male 25-d-old Wistar rats were assigned to one of six groups: sedentary rats fed with chow diet; exercised (swimming 90 min/d, 5 d/wk) rats fed with chow diet; sedentary rats fed with a palatable hyperlipidic diet; exercised rats fed with the palatable hyperlipidic diet; sedentary rats fed with food cycles (four cycles alternating the chow and hyperlipidic diets weekly); and exercised rats fed with food cycles. After 8 wk of treatment, the animals were killed 24 h after the last exercise session. RESULTS: The hyperlipidic diet and food cycles schedules caused similar increases in body weight gain, carcass lipogenesis rate and adiposity, lipid content of the liver and gastrocnemius muscle, and serum total lipid, triacylglycerol, insulin, and leptin levels. The exercise attenuated body weight gain, adipose tissue mass, and serum triacylglycerol, insulin, and leptin levels similarly in the hyperlipidic and food cycles groups. Carcass lipogenesis rate was not affected by exercise in any of the three groups. CONCLUSIONS: The data showed that the continuous intake of a hyperlipidic palatable diet for 8 wk and the alternation of the high-fat intake with periods of chow intake cause obesity and affected lipid metabolism in a similar way. Chronic exercise attenuated body weight gain and adiposity and improved serum lipid concentrations in both high-fat feeding regimens.     

Effects of a Regional Chinese Diet on Proliferation of Human Esophageal Cancer Cell Line Eca-109 by a Sero-Physiology Method

Esophageal squamous cell carcinoma (ESCC) is prevalent in Yanting (YT) country located in southwestern China. Residents in the YT region have an unusual diet pattern and the role of the YT diet on the risk of ESCC is still uncertain. The present study was to examine the possible effects of sera from rats fed with the YT diet on proliferation of human esophageal cancer cell line Eca-109 by means of a sero-physiology approach. Firstly, two feasibilities were assessed to set up the sero-physiology method. We found that rats fed with a human adult diet in Chengdu region (ESCC-low-risk-area) for 30d had very close body weight gains in comparison with the control rats fed with the conventional diet, confirming the feasibility of feeding rats with a human diet without affecting their normal growth. Cell growth results showed that 5% non-deactivated rat serum had exactly the same effect on the proliferation of Eca-109 cells compared with the fetal bovine sera (FBS) control, confirming the feasibility of cultivating Eca-109 cells with the rat serum instead of FBS. Subsequently, cell proliferation results indicated that rats’ sera fed with the YT diet significantly promoted Eca-109 cells proliferation but inhibited human normal liver epithelial cell line control HL7702 proliferation, whereas rats’ sera fed with the Chengdu diet didn't have these effects on the two cell lines. The different effects of the two human diets on proliferation of Eca-109 cells demonstrated that the sero-physiology method is effective in studying the relationship between diet and cancer, and there maybe exist cancer-promoting factors in the YT diet.     

亮氨酸对高脂膳食喂养大鼠胰岛素敏感性的影响

目的探讨亮氨酸(Leucine,Leu)对高脂膳食诱导的胰岛素抵抗(insulin resistance,IR)大鼠胰岛素敏感性的影响。方法给正常大鼠和高脂膳食大鼠分别补充0,1.5%,3.0%和4.5%水平的亮氨酸24w。进行胰岛素耐量实验。检测血清Leu水平、血糖,血胰岛素,计算胰岛素抵抗指数(HOMA-IR)。Western blot检测肝脏哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及磷酸化mTOR表达水平。结果高脂喂养大鼠Leu干预组胰岛素耐量曲线下面积值(AUC)明显降低,血胰岛素水平及HOMA-IR明显降低。Leu可显著增强胰岛素刺激后肝脏pSer2448 mTOR的表达。Leu补充对正常大鼠上述指标无明显影响。结论膳食补充Leu可以增强高脂喂养胰岛素抵抗大鼠的胰岛素敏感性。[营养学报,2012,34(6):545-548,552]     

The effect of treadmill exercise on azoxymethane-induced intestinal neoplasia in the male Fischer rat on two different high-fat diets

A total of 120 eight-week-old male rats were exposed to azoxymethane (15 mg/kg body wt in saline s.c.) on Days 1, 4, and 8. Two days after the last injection of carcinogen, the rats were randomized into four experimental groups: two groups were given a chow high in corn oil (23% corn oil) and two groups a chow high in coconut oil (21% coconut oil and 2% corn oil). One group on each chow was kept sedentary, and one group was exposed to moderate exercise, running 2 km/day on weekdays for 38 weeks. At the end of the experiment, the exercising and sedentary rats fed coconut oil were significantly heavier than those fed corn oil. Among the rats fed the high-fat corn oil diet, exercise reduced the number of animals developing carcinomas in the colon (sedentary, 10; exercise, 0) and in the small intestine (sedentary, 5; exercise, 0). The same tendency was observed in the rats fed the coconut oil diet: colon (sedentary, 6; exercise, 3) and small intestine (sedentary, 3; exercise, 2). In the sedentary rats fed the high corn oil diet, 16 intestinal carcinomas were recorded; none were found in the exercised group. In the rats fed the high coconut oil diet, nine carcinomas were recorded in the sedentary groups as opposed to five in the exercised rats. Rats fed the high-fat coconut oil developed significantly fewer neoplasms than the rats fed the equally high-fat corn oil diet.     

缺锌对胸腺和脾脏细胞增殖周期DNA及蛋白质含量的影响

通过建立大鼠缺锌（ＺＤ）模型，采用流式细胞术，研究缺锌对胸腺、脾脏细胞增殖周期、ＤＮＡ及蛋白质含量的影响。结果表明：（１）ＺＤ组的最终体重、体重增长值、饲料效价、以及血清、毛发、股骨锌均非常显著地低于缺锌对喂（ＰＦ）组。（２）ＺＤ组胸腺、脾细胞的增殖指数和Ｇ＿２＋Ｍ期细胞的百分率非常显著地低于ＰＦ组；胸腺、脾脏的Ｇ０／Ｇ＿１期细胞百分率却非常显著地高于ＰＦ组。（３）ＺＤ组胸腺、脾脏细胞的ＤＮＡ含量、ＤＮＡ指数、蛋白质含量、蛋白质指数显著低于ＰＦ组。缺锌时，ＤＮＡ复制和蛋白质合成受到抑制，致使细胞停滞于Ｇ０／Ｇ＿１期，仅少量进入Ｓ期和Ｇ＿２＋Ｍ期、使增殖指数减低。     

同型半胱氨酸促进血管平滑肌细胞增生的实验研究

目的 :　研究同型半胱氨酸 (homocysteine,Hcy)是否促进平滑肌细胞增生。方法 :　SD大鼠主动脉平滑肌细胞体外培养 ,随机分为 5组 :1 .正常对照组 ,2 .1 .0 mmol/L Hcy组 ,3 .2 .5 mmol/L Hcy组 ,4.5 .0 mmol/L Hcy组 ,5 .1 0 .0 mmol/L Hcy组。培养 72 h后 ,采用细胞毒试验、流式细胞仪检测细胞生长情况。 2 0只 SD大鼠进行体内观察 ,随机分为高蛋氨酸组 (M组 )和对照组 (C组 ) ,M组每日给予含蛋氨酸 3 %的饮食 ,在 1 2 w进行主动脉病理学光镜和电镜检查。结果 :　 1 .细胞毒试验观察到 Hcy促进平滑肌细胞生长与增殖 ;2 .流式细胞仪检测可见 Hcy促进平滑肌细胞由 G1期进入合成的 S期 ;3 .在 4w时 ,高蛋氨酸组大鼠即出现高同型半胱氨酸血症 ,高蛋氨酸血症 ;4.病理检查光镜下可见高蛋氨酸组大鼠主动脉有明显中膜增厚 ,平滑肌细胞增生 ;5 .电镜检查可见平滑肌细胞肿胀 ,内质网扩张 ,线粒体水肿 ,溶酶体增多。结论 :　同型半胱氨酸促进大鼠血管平滑肌细胞增生     

Influence of dietary fat intake on local recurrence and progression of metastases arising from MDA-MB-435 human breast cancer cells in nude mice after excision of the primary tumor.

This study was performed to evaluate the effect of dietary fat on the recurrence and metastasis of human breast cancer solid tumors growing in nude mice after surgical excision of the primary tumor. Female nude mice were fed either a high- (23% corn oil) or a low-fat (5% corn oil) diet, and 7 days later 1 x 10(6) MDA-MB-435 human breast cancer cells were injected into a thoracic mammary fat pad. Tumors at the injection site grew more rapidly in the animals fed the high-fat diet. Nineteen of 30 animals in each dietary group had tumors with a surface are > or = 1 cm2 within 10 weeks of injection, at which point the tumors were excised and the animals were followed for another eight weeks. Tumors recurred at the excision site in 8 of 19 animals fed the high-fat diet and in 9 of 19 animals fed the low-fat diet; however, the growth rate was more rapid in the group fed the high-fat diet. Lung metastases occurred with similar frequency in the two groups with local recurrences, but with a positive correlation between recurrent tumor weight (greater in the animals fed the high-fat diet) and the severity of lung metastatic involvement. In the mice without recurrence, 4 of 11 (36%) animals in the group fed the high-fat diet had macroscopic lung metastases compared with only one mouse, with minimal involvement, in the group fed the low-fat diet.