均匀设计优选智能型密闭敷料支架基质配方

背景：近年来创伤敷料的研究发展较快,但很少有中药联合高分子材料制备的复合密闭敷料,且现有的创伤敷料作用较局限,不能适用于任何创伤伤口,但怎样使高分子材料与中药完美的融合是目前一个热门的话题。目的：优选智能型密闭敷料支架的最佳基质配方。方法：采用均匀设计法结合SPSS16.0软件进行多元线性回归分析,对敷料支架的吸水性、含水量、保湿性和外观评价等指标进行综合考察,优化智能型密闭敷料支架的基质设计。结果与结论：通过实验数据分析,优选出智能型密闭敷料支架最佳基质配方比例为：壳聚糖：明胶：甘油：聚乙烯醇：聚乙二醇=8.3：4.4：3.1：3.7：1。提示智能型密闭敷料支架基质处方配比合理、制作工艺质量可控。     

猪松质骨支架/骨髓基质干细胞复合体兔体内成骨

目的:生物衍生骨具有天然骨组织的三维立体孔隙.网架结构,有利于种子细胞的黏附、增殖.观察猪松质骨支架/骨髓基质干细胞复合体在兔体内成骨及支架的降解.方法:实验于2005-03/2006-12在中南大学湘雅医院中心实验室完成.选择健康家兔16只,3月龄左右,其中1只提供骨髓,另15只作组织工程骨植入,分4,8,12周3个时间点,每个时间点5只.采用Ficoll密度梯度离心法得到兔骨髓基质干细胞并诱导培养:采用物理化学方法对猪松质骨进行处理得到猪松质骨支架,与经诱导培养的骨髓基质干细胞体外复合培养后植入机体内,同时植入单纯猪松质骨支架做自身对照.结果:纳入动物15只,均进入结果分析.采用X射线、苏木精-伊红染色、Masson三色染色及图像分析观察猪松质骨支架屑髓基质干细胞复合体在兔体内成骨及支架的降解.①X射线观察:猪松质骨支架/骨髓基质干细胞复合体移植术后4周移植区周围可见少量散在分布的中密度影,8,12周时中高密度影逐渐向中心区增大.单纯猪松质骨支架移植术后各时间点移植区内均呈低密度影.②组织学观察:猪松质骨支架屑髓基质干细胞复合体植入后4周开始有新骨生成,支架开始降解;8周时新骨的形成及支架的降解都明显增加;12周时新骨继续增加,逐渐向成熟骨组织转变,可见少量哈佛系统,但骨小梁尚不典型,毛细血管增生明显,少量支架残留,未见炎症细胞.单纯猪松质骨支架植入后各时间点无新骨形成.③图像分析表明:猪松质骨支架/骨髓基质干细胞复合体植入后新骨组织随时间延长而增加,而材料组织随时间延长而减少,但新生骨组织增加与支架材料组织减少无明显直线相关(P＞0.05).结论:猪松质骨支架与骨髓基质干细胞复合移植后新骨逐渐形成,支架逐渐降解,是一种较理想的骨组织工程支架材料.     

密闭性敷料支架的制备及其性能

背景:近年来创伤敷料的研究发展较快,但很少有中药联合高分子材料制备的复合密闭敷料,且现有的创伤敷料作用较局限,不能适用于任何创伤伤口.目的:制备适用于中药载入的密闭敷料支架,并测定其性能.方法:将壳聚糖、明胶、聚乙烯醇,壳聚糖、明胶、聚乙烯醇和海藻酸钠分别在一定条件下共混,预冻,冷冻干燥得到支架,考察两支架的吸水性、含水量、相对保湿性、透气性、孔隙率、支架的外形.结果与结论:壳聚糖、明胶、聚乙烯醇所制成支架的吸水性、含水量、相对保湿性、孔隙率优于壳聚糖、明胶、聚乙烯醇和海藻酸钠所制成的支架,两组比较差异有显著性意义(P<0.05).说明壳聚糖、明胶、聚乙烯醇适宜作为密闭敷料支架材料.     

密闭性功能敷料智能结构材料选择的分析

密闭性功能敷料面世以来,应用范围及功能开发日新月异,研究表明其应用范围的大小及功能性的强弱直接取决于敷料的智能支架结构的承载物质及其根据环境而产生的自身的调控能力.本文综述了密闭性功能敷料智能结构的材料选择及作用方式.     

Electrospun acellular scaffolds for mimicking the natural anisotropy of the extracellular matrix

In tissue engineering, the use of scaffolds helps establish a synergistic relationship between the scaffolds and the tissues by improving cell–scaffold interaction. This interaction is enhanced when physiologically relevant biophysical cues are replicated in the artificial scaffolds. Here, we present a novel scaffold that mimics the natural anisotropy of the native extracellular matrix of tissues, fabricated by electrospinning a combination of three polymers: polycaprolactone (PCL), polyvinylidene fluoride (PVDF) and polyaniline (PANI). The scaffolds were characterized for their morphology, surface and mechanical properties. Rat cardiomyoblast (H9c2) cells, cultured on the PCL–PANI–PVDF scaffold, demonstrated cell alignment, penetration and proliferation across the entire surface area of the scaffold without any external chemical or physical stimuli. The PCL–PANI–PVDF scaffold, unlike other scaffolds, does not require post-processing or specific temperature conditions of storage, prior to use. These acellular scaffolds fabricated through polymer blending, open new avenues for research on functional acellular scaffolds for tissue engineering, based on synthetic materials.

Co-axial electrospinning of three immiscible polymers by sequentially blending in solvents of different boiling points to obtain biomimetic scaffolds.     

脱细胞猪真皮基质敷料和其他生物敷料的透水特性

目的:比较不同生物敷料的透水特性及各种创面所需敷料的透水特性。方法:实验于2003-01/04在佛山市第一人民医院烧伤科实验室完成。采用透湿杯法测试启东脱细胞猪真皮基质敷料、启东脱细胞猪真皮基质敷料+PVC薄膜、人体全层异体皮、人体刃厚皮、去表皮真皮深层断层猪皮、人体三度烧伤焦痂、人体深二度烧伤痂皮、水合纤维素敷料、戊二醛全层猪皮、Intergra、新鲜去表皮猪皮(含全部真皮层)、Alloderm、新鲜全层腹部猪皮、新鲜全层背部猪皮、启东脱细胞猪真皮基质敷料+8层纱布、启东脱细胞猪真皮基质敷料+16层纱布及PVC薄膜的透水量。结果:①在透湿杯内15m L生理盐水干水时间:启东脱细胞猪真皮基质敷料2d,人体刃厚皮40d,去表皮真皮深层断层猪皮、戊二醛全层猪皮、启东脱细胞猪真皮基质敷料+8层纱布和启东脱细胞猪真皮基质敷料+16层纱布为2.5d或3d,启东脱细胞猪真皮基质敷料+PVC薄膜、人体异体皮、新鲜全层腹部猪皮、PVC薄膜40d均未干。②透水性(24h,37℃下):启东脱细胞猪真皮基质敷料为5856.56g/m2,启东脱细胞猪真皮基质敷料+PVC薄膜413.21g/m2,人体异体皮1194.25g/m2,人体刃厚皮249.12g/m2,去表皮真皮深层断层猪皮611.47g/m2,戊二醛全层猪皮6100.36g/m2,新鲜全层腹部猪皮780.52g/m2,启东脱细胞猪真皮基质敷料+8层纱布3584.53g/m2,启东脱细胞猪真皮基质敷料+16层纱布4644.95g/m2,PVC薄膜1777.86g/m2。结论:不同生物敷料的透水性数据对临床不同创面的生物敷料的选择具有指导意义,表皮、异体皮保湿性良好,可用于切削痂创面的保护;启东脱细胞猪真皮基质敷料透水透气性良好,可用于浅Ⅱ度和深Ⅱ度创面保护。     

脱细胞猪真皮基质敷料和其他生物敷料的透水特性

目的：比较不同生物敷料的透水特性及各种创面所需敷料的透水特性.方法：实验于2003-01/04在佛山市第一人民医院烧伤科实验室完成.采用透湿杯法测试启东脱细胞猪真皮基质敷料、启东脱细胞猪真皮基质敷料＋PVC薄膜、人体全层异体皮、人体刃厚皮、去表皮真皮深层断层猪皮、人体三度烧伤焦痂、人体深二度烧伤痂皮、水合纤维素敷料、戊二醛全层猪皮、Intergra、新鲜去表皮猪皮（含全部真皮层）、Alloderm、新鲜全层腹部猪皮、新鲜全层背部猪皮、启东脱细胞猪真皮基质敷料＋8层纱布、启东脱细胞猪真皮基质敷料＋16层纱布及PVC薄膜的透水量.结果：①在透湿杯内15 mL生理盐水干水时间：启东脱细胞猪真皮基质敷料2 d,人体刃厚皮40 d,去表皮真皮深层断层猪皮、戊二醛全层猪皮、启东脱细胞猪真皮基质敷料＋8层纱布和启东脱细胞猪真皮基质敷料＋16层纱布为2.5 d或3 d,启东脱细胞猪真皮基质敷料＋PVC薄膜、人体异体皮、新鲜全层腹部猪皮、PVC薄膜40 d均未干.②透水性（24 h,37℃下）：启东脱细胞猪真皮基质敷料为5 856.56 g/m^2,启东脱细胞猪真皮基质敷料＋PVC薄膜413.21 g/m,人体异体皮1 194.25 g/m^2,人体刃厚皮249.12g/m^2,去表皮真皮深层断层猪皮611.47 g/m^2,戊二醛全层猪皮6 100.36g/m^2,新鲜全层腹部猪皮780.52g/m^2,启东脱细胞猪真皮基质敷料＋8层纱布3 584.53 g/m^2,启东脱细胞猪真皮基质敷料＋16层纱布4 644.95 g/m^2,PVC薄膜1 777.86g/m^2.结论：不同生物敷料的透水性数据对临床不同创面的生物敷料的选择具有指导意义,表皮、异体皮保湿性良好,可用于切削痂创面的保护;启东脱细胞猪真皮基质敷料透水透气性良好,可用于浅Ⅱ度和深Ⅱ度创面保护.     

Improved physical and osteoinductive properties of demineralized bone matrix by gelatin methacryloyl formulation

The demineralized bone matrix (DBM) is the most widely used bone allograft, which is obtained by removing the mineral component of bone, leading to exposure of the proteins responsible for osteoinduction. For clinical use, DBM shall be formulated with a carrier that provides consistency and improves its osteoinduction. In this study, three DBM formulations with glycerol (Gly), hyaluronic acid (HA), and gelatin methacryloyl (GelMA) were evaluated measuring their physicochemical properties (microstructure, compressive strength, and serum cohesivity) and their osteoinductive capacity both in vitro using C2C12 cells and umbilical cord human mesenchymal stem cells and in vivo in an ectopic bone formation model in athymic mice. To assess the effectiveness of DBM in vitro in inducing the differentiation into osteoblasts, alkaline phosphatase (ALP) activity was assessed in combination with a cytotoxicity assay. In vivo, new bone formation was assessed by histological and radiological analysis. In the compression and in the serum cohesive assays, the GelMA DBM formulation showed its superiority over the other formulations. In addition, GelMA showed a more compact structure analysed by scanning electron microscopy. Higher cell toxicity was observed on Gly formulations in vitro, whereas GelMa and HA showed very low toxicity. All formulations significantly improved ALP activity compared with control. In the in vivo studies, GelMA showed the greatest osteoinductive potential with a higher percentage of new bone and bone marrow formation. Our results suggest GelMA is useful as a carrier for DBM designed to promote the formation of the new bone.     

Collagen matrix scaffolds: Future perspectives for the management of chronic liver diseases

Approximately 1.5 billion chronic liver disease(CLD) cases have been estimated worldwide, encompassing a wide range of liver damage severities. Moreover, liver disease causes approximately 1.75 million deaths per year. CLD is typically characterized by the silent and progressive deterioration of liver parenchyma due to an incessant inflammatory process, cell death, over deposition of extracellular matrix proteins, and dysregulated regeneration. Overall, these processes impair the correct functio...     

拟水平均匀设计法优化超声联合微泡促人骨髓间充质干细胞分泌SDF-1的研究

目的 采用拟水平均匀实验设计,探讨超声联合微泡促人骨髓间充质干细胞分泌SDF-1的最优参数组合。方法 选定超声辐照强度、超声辐照时间、微泡浓度三个因素,又分别按超声辐照强度 0 (对照)、0.2、0.4、0.6、0.8 W/cm2,辐照时间 0 (对照)、20、30、40、60 s,微泡浓度 0 (对照)、102、104、106、108 N/ml,每因素五个水平设计均匀设计表,根据均匀设计表U15(155) 进行超声辐照,辐照后细胞继续培养24 h,然后检测细胞存活率及细胞培养上清液中的SDF-1浓度。以较高的SDF-1浓度和较低的细胞死亡率为目标,应用多元线性回归和逐步回归分析确定最佳参数配比,并对统计分析后的优化参数再进行实验验证。结果 综合考虑各因素间关系及回归分析结果后,确定超声联合微泡促人骨髓间充质干细胞分泌SDF-1的最优参数组合为辐照强度 0.6 W/cm2,辐照时间 30 s,微泡浓度 106/ml,验证实验显示在此条件下超声辐照后 SDF-1分泌量达 551.67±40.88 pg/ml,细胞存活率达 88.51±4.03%。结论 应用拟水平均匀设计可有效优化参数条件,使超声辐照后间充质干细胞的SDF-1分泌量和细胞存活率达到相对匹配的数值。