生脉饮加味治疗病毒性心肌炎

病毒性心肌炎是临床常见病，多发病，近年来发病率有上升趋势，且部分病人不愈转为慢性过程对人们的健康造成较大危害，西药目前对本病尚无特效疗法。我院2001年6月～2004年12月运用中药生脉饮加味治疗病毒性心肌炎66例，取得较满意疗效，现报道如下。     

生脉饮口服液制备工艺改进

通过制备工艺改进,解决了生脉饮口了液灭菌放后产生沉淀的问题,经稳定性考察,ＴＬＣ和含量测定分析,改进工艺与药典工艺制备的生脉饮口服液成分基本相同。     

Effect of Shengmai Yin on Epithelial-Mesenchymal Transition of Nasopharyngeal Carcinoma Radioresistant Cells

Objective: To investigate the mechanism by which Chinese medicine Shengmai Yin(SMY)reverses epithelial-mesenchymal transition(EMT) through lipocalin-2(LCN2) in nasopharyngeal carcinoma(NPC)cells CNE-2R. Methods: Morphological changes in EMT in CNE-2R cells were observed under a microscope,and the expressions of EMT markers were detected using quantitative real-time PCR(RT-q PCR) and Western blot assays. Through the Gene Expression Omnibus dataset and text mining, LCN2 was found to be highly rela...     

生脉饮对小鼠生长发育和学习记忆的促进作用

目的：探讨中药古方生脉饮及其成分对小鼠生长发育和学习记忆方面的作用。方法：ａ）采用直接测量小鼠体重、脑重、大脑皮层厚度和饲料转化率的方法来研究生脉饮及其成分对小鼠生长发育的作用;ｂ）采用三等分Ｙ型迷宫作为学习记忆研究的行为模式,研究生脉饮及其成分对学习记忆的作用及对ＮａＮＯ２和乙醇所致记忆障碍的保护作用。结果：生脉饮及其成分对小鼠体重、脑重、大脑皮层厚度及饲料转化率有促进作用,与对照组比较有显著差     

Observation on therapeutic effects of Shengmai powder in treating acute viral myocarditis

Objective: To evaluate the clinical efficacy of Shengmai Powder (SMP) in treating acute viral myocarditis objectively.Methods: One hundred and twenty-four patients with acute viral myocarditis were randomized into the treated group (SMG,n = 64) and the control group (CG,n = 60). Such myocardial nutrient medicine as ATP, CoA, Vit-C, were given to both groups. And to the treated group, 40 ml of Shengmai Injection per day was given intravenously for 2 weeks, which was followed by oral intake of Shengmai granule, one package three times daily for another 2 weeks in total. The same anti-arrhythmia agents were applied to both groups, and no fructose-1, 6-diphosphate (FDP) for either. Semi-quantitative scoring method was adopted to observe such symptoms as chest stuffiness, palpitation and chest pain before treatment and four weeks after treatment. Meanwhile, ECG, dynamic ECG by Holter monitor, left ventricular enddiastolic dimension (LVEDD), left ventricular ejection fraction (LVEF), serum neutralizing antibody of virus Coxsackie B, cardiac troponin I (cTnl) and cardiac troponin T (cTnT) were examined.Results: (1) Compared with the control group, more significant improvement was got in SMG in respects of chest stuffiness, palpitation, chest pain and arrhythmia (P<0.05 orP<0.01). (2) Negative converting rates of cTnl,cTnT in the two groups were 59.46% vs 35.48%, 68.75% vs 42.31% respectively (P<0.05). (3) LVEDD before and after treatment in SMG was 52. 44 ± 3. 40 mm and 48. 81 ± 2. 23mm respectively, while that in the control group was 52.31 ± 3. 74 mm and 49. 92 ± 2. 67mm respectively; LVEF before and after treatment in SMG was 60.67 ± 4.62% and 65. 02 ± 4.16% respectively, while that in the control group was 60. 91± 4.26% and 63.67 ± 3.17%. There was obvious improvement in the two parameters in both groups, but the improvement in SMG was superior to that in the control group (P<0.05).Conclusion: SMP shows a good effect in improving clinical symptoms and signs, heart function, abnormal ECG and inflammatory injury indexes in patients with acute viral myocarditis. This project was enlisted as one item of the National Ninth 5-Year Projects (No. 96-90602-13)     

Effect of Shengmai Yin on the DNA methylation status of nasopharyngeal carcinoma cell and its radioresistant strains

Shengmai Yin (SMY) is a Chinese herbal decoction that effectively alleviates the side effects of radiotherapy in various cancers and helps achieve radiotherapy''s clinical efficacy. In this study, we explored the interaction mechanism among SMY, DNA methylation, and nasopharyngeal carcinoma (NPC). We identified differences in DNA methylation levels in NPC CNE-2 cells and its radioresistant cells (CNE-2R) using the methylated DNA immunoprecipitation array and found that CNE-2R cells showed genome-wide changes in methylation status towards a state of hypomethylation. SMY may restore its original DNA methylation status, and thus, enhance radiosensitivity. Furthermore, we confirmed that the differential gene Tenascin-C (TNC) was overexpressed in CNE-2R cells and that SMY downregulated TNC expression. This downregulation of TNC inhibited NPC cell radiation resistance, migration, and invasion. Furthermore, we found that TNC was hypomethylated in CNE-2R cells and partially restored to a hypermethylated state after SMY intervention. DNA methyltransferases 3a may be the key protein in DNA methylation of TNC.     

生脉饮治疗气阴两虚型肺结核并多汗症临床研究

目的：观察生脉饮治疗气阴两虚型肺结核并多汗症的临床疗效。方法：40例肺结核患者随机平均分为两组。治疗组20例，在常规抗结核治疗基础上给予生脉饮口服液，每次10 mL，每日3次，口服。对照组20例，在常规抗结核治疗基础上给予谷维素，每次30 mg，每日3次，口服。30 d为1个疗程，1个疗程后统计疗效。结果：两组临床疗效比较：治疗组治愈率为60.0%，对照组治愈率为30.0%，两组比较，差异有统计学意义（ P<0.05）。肺部病灶变化比较：治疗组有效率为75.0%，对照组有效率为65.0%，两组比较，差异有统计学意义（P<0.05）。痰菌阴转比较：治疗组阴转率68.8%，对照阴转率64.3%，两组比较，差异无统计学意义（ P>0.05）。结论：生脉饮治疗气阴两虚型肺结核并多汗症临床疗效较好。     

Observation on Therapeutic Effects of Shengmai Powder(生脉散)in Treating Acute Viral Myocarditis

Objective: To evaluate the clinical efficacy of Shengmai Powder (SMP, 生脉散) in treating acute viral myocarditis objectively. Methods: One hundred and twenty-four patients with acute viral myocarditis were randomized into the treated group (SMG, n=64) and the control group(CG, n=60 ). Such myocardial nutrient medicine as ATP, CoA, Vit-C, were given to both groups. And to the treated group, 40 ml of Shengmai Injection per day was given intravenously for 2 weeks, which was followed by oral intake of Shengmai granule, one package three times daily for another 2 weeks in total. The same anti-arrhythmia agents were applied to both groups, and no fructose-1, 6-diphosphate (FDP) for either. Semi-quantitative scoring method was adopted to observe such symptoms as chest stuffiness, palpitation and chest pain before treatment and four weeks after treatment. Meanwhile, ECG, dynamic ECG by Holter monitor, left ventricular enddiastolic dimension (LVEDD), left ventricular ejection fraction (LVEF), serum neutralizing antibody of virus Coxsackie B, cardiac troponin I (cTnl) and cardiac troponin T (cTnT) were examined. Results: (1) Compared with the control group, more significant improvement was got in SMG in respects of chest stuffiness, palpitation, chest pain and arrhythmia ( P＜0.05 or P＜0.01). (2) Negative converting rates of cTnl , cTnT in the two groups were 59.46% vs 35.48%, 68.75% vs 42.31% respectively (P＜0.05). (3) LVEDD before and after treatment in SMG was 52.44±3.40 mm and 48.81± 2.23mm respectively, while that in the control group was 52.31±3.74 mm and 49.92±2.67mm respectively; LVEF before and after treatment in SMG was 60.67±4.62 % and 65.02±4.16 % respectively, while that in the control group was 60.91± 4.26 % and 63.67±3.17 %. There was obvious improvement in the two parameters in both groups, but the improvement in SMG was superior to that in the control group (P＜0.05). Conclusion: SMP shows a good effect in improving clinical symptoms and signs, heart function, abnormal ECG and inflammatory injury inde xes in patients with acute viral myocarditis.     

从脏腑气血阴阳辨治糖尿病性肾病

糖尿病性肾病其病机为虚实夹杂,本虚为肝、脾、肾气血阴阳失调;标实多为燥热、湿浊、瘀血、水毒。根据疾病所处的病理阶段及临床表现,可分为阴虚燥热证、肝肾阴虚证、气阴两虚证、阴阳两虚证、脾肾阳虚证及肾阳衰微证等6型,治疗上强调早期治疗,辨证施治,兼顾标本虚实,发挥中医药治疗本病的优势,使病情长期稳定,延缓疾病进程。     

加味生脉二至汤对免疫介导再生障碍性贫血模型肠系膜微循环的实验研究

目的：观察加味生脉二至汤对免疫介导的再生障碍性贫血（IAA）小鼠微循环的改善作用，为临床用药提供依据。方法：取自DBA／2小鼠的胸腺淋巴结单细胞悬液，输入经亚致死剂量γ－射线照射后的Balb/c小鼠，制作IAA模型，分别予以生理盐水和不同剂量的加味生脉二至汤方煎出液灌胃。于给药第4、8和10天麻醉下活体观察肠系膜微血管的状态（管径、流态等）。结果：（1）血管管径：模型组、药物Ⅰ组、药物对照组低于正常组，有明显差异（P＜0.05）。给药Ⅱ组与正常组相比无显著性差异；（2）血液流态：给药组明显优于模型组（P＜0.05），给药组组间无明显差异（P＞0.05）。结论：实验用加味生脉二至汤能改善再生障碍性贫血（简称再障）小鼠模型肠系膜微血管的血流状态。     

生脉注射液对心肺复苏兔海马神经元超微结构的影响

[目的 ]探讨生脉注射液在心肺复苏中的应用价值及时机。 [方法 ]健康大白兔 30只 ,随机分为 5组 (每组 6只 ) :A组为常规复苏组 ;B组在常规复苏同时加入生脉注射液 5ml;C组在常规复苏开始 5min后 ,加入生脉注射液 5ml;D组在常规复苏开始 10min后加入生脉注射液 5ml;E组在常规复苏开始 15min后加入生脉注射液 5ml。采用窒息法制成心脏骤停动物模型 ,进行心肺复苏 ,电镜观察大脑海马神经元超微结构的变化。 [结果 ]复苏后各组海马神经元细胞的超微结构均存在不同程度的病理改变 ,但各组之间存在差异 ,A组病变最严重 ,B组病变最轻 ,C组、D组、E组病变依次加重 ,但均比A组轻。 [结论 ]生脉注射液可以减轻复苏兔海马神经元细胞超微结构的病理损害 ,且与应用时机有一定的关系 ,应用越早 ,损害越轻。     

丹参冻干粉预冻温度的研究

目的测定不同浓度丹参药液的最低预冻温度。方法电阻法测定不同浓度丹参药液共晶点,进而判断丹参药液预冻温度。结果 100、150、200 g/L丹参药液共晶点分别为-19、-23、-28.8℃,对应药液最低预冻温度为-24~-29℃,-28~-33℃,-33.8~-38.8℃。结论在判定的最低预冻温度下,冻干产品性状良好,指标成分含量符合质量标准的要求。     

生脉注射液细菌内毒素检查法研究

通过初步筛选试验和干扰试验对生脉注射液的细菌内毒素检查法进行了研究,结果表明,生脉注射液经10倍稀释,用灵敏度为0.25 EU·mL-1的鲎试剂进行细菌内毒素检查无干扰作用.     

生脉注射液对休克复苏的影响

目的：探讨生脉注射液在休克复苏中对血管活性药物用量及抢救时间的影响。方法：60例休克病例随机分为两组,每组30例。两组病例均采取扩容、应用血管活性药物、病因治疗、对症治疗等常规治疗,治疗组在对照组治疗的基础上,给予生脉注射液治疗。观察两组患者血管活性药物用量与抢救时间以及抢救过程中血压、心率的变化。结果：治疗组多巴胺维持量、维持时间、总用量及抢救时间均明显低于对照组,治疗组用药后2 h的收缩压、3 h的收缩压、舒张压、平均动脉压、脉压差均明显高于对照组,治疗组用药后3 h心率明显低于对照组,差异有统计学意义（P〈0.05）。结论：生脉注射液对休克病人具有升压稳压作用,可以减少血管活性药物的用量,缩短休克抢救时间,对抢救休克有明显辅助效果。     

生脉散对阿霉素心肌毒性的拮抗作用

本文通过大鼠实验探讨了生脉散对阿霉素心脏毒性的拮抗作用。结果在明：生脉散组大鼠ＡＤＲ毒性症状出现较晚、发生充血性心力衰竭例数较ＡＤＲ组少，且心肌病理改变明显较ＡＤＲ组轻。另外，ＡＤＲ对大鼠全血ＧＳＨ－Ｐｘ活性及心肌ＳＯＤ含量有明显的抑制作用，使心肌组织内ＭＤＡ合量增加，而生脉散组大鼠全血ＧＳＨ－ＰＸ、ＳＯＤ活性较ＡＤＲ组增高，ＭＤＡ含量较ＡＤＲ组减少（Ｐ＜０．０１～０．０５）。提示生脉散对ＡＤＲ所制心肌毒性具有拮抗作用，其机理可能与抗自由基反应有关。     

生脉散联合倾斜训练对心内科老年血管迷走神经性晕厥患者疗效及预后的影响

目的 探究生脉散联合倾斜训练对心内科老年血管迷走神经性晕厥(VS)患者疗效及预后的影响。 方法 选取2015-2017年间在西溪医院进行VS治疗的患者80例作为研究对象,采用随机数字法分为2组。对照组(40例)给予常规对症治疗,观察组(40例)在此基础上给予生脉散联合倾斜训练治疗,治疗3个月,对比2组患者治疗前后舌下硝酸甘油倾斜试验结果,血流动力学变化以及血浆儿茶酚胺水平,随访6个月观察药物副作用并比较2组患者晕厥症状复发率。 结果 观察组在倾斜前的基础收缩压、舒张压和心率在治疗前后的差异无统计学意义(P>0.05);治疗后,晕厥或终止试验时的收缩压、舒张压和心率均较治疗前明显升高(均P<0.05);治疗前,观察组在晕厥发作时伴有血浆肾上腺素和去甲肾上腺素水平显著升高,与平卧时差异有统计学意义(P<0.05);治疗后,晕厥或终止试验时患者血浆肾上腺素和去甲肾上腺素水平与平卧时相比差异无统计学意义(P>0.05);治疗3个月后,观察组患者HUTT试验转阴率显著高于对照组,差异有统计学(P<0.05);随访6个月,2组晕厥复发率差异有统计学意义(P<0.05),观察组与对照组平均晕厥发作次数差异有统计学意义(P<0.05); 结论 生脉散联合倾斜训练用于老年血管迷走神经性晕厥的治疗,能够改善患者血流动力学,降低血浆儿茶酚胺水平,有效减少晕厥发作次数,预防晕厥复发,值得临床推广。