血清中白细胞介素6和白细胞介素8水平在肝病检测中的临床应用

目的:血清中IL-6和IL-8的含量是临床检验中的重要指标,本实验主要对肝癌患者血清的IL-6和IL-8的水平进行了统计分析,为进一步临床监测提供依据。方法:对86份肝癌患者血清进行检测,同时收集30份正常血清和35份肝硬化患者血清作为对照比较。结果:肝癌患者血清中IL-6水平[(0.197±0.041)ng/ml]显著高于对照组(正常组的0.159±0.038;肝硬化组0.185±0.026);肝癌患者血清中IL-8水平[(0.463±0.127)ng/ml],显著高于对照组(正常组的0.354±0.113;肝硬化组,0.386±0.102)。结论:血清中IL-6、IL-8水平可以作为肝癌的临床监测指标。     

白细胞介素-8与分泌性中耳炎

分泌性中耳炎(Otitis Media with Effusion,OME)又称非化脓性中耳炎,是以中耳渗液及听力下降为主要表现.此病是以中耳渗出液潴留伴听力下降为主要表现的中耳常见病、多发病.该病成人及儿童均可发病,但以儿童发病率较高.持续的0ME常导致儿童听力损害,使幼儿语言发育及心理健康受到影响.OME的主要病因包括咽鼓管功能障碍、感染、免疫反应等方面.由于白细胞介素-8(IL-8)具有趋化白细胞的作用,它对OME中炎症的持续及粘液的产生什么影响,越来越受到学者们的关注.现就近年来对IL-8与分泌性中耳炎病程关系的研究作一综述.     

白细胞介素-8在大肠杆菌中的克隆和表达

白细胞介素┐８在大肠杆菌中的克隆和表达苏琴王嘉玺邹民吉肖定华赵春文王利红段聚宝周俐梅刘海天军事医学科学院基础医学研究所北京１００８５０白细胞介素－８（ＩＬ－８）是一种由内皮细胞和单核细胞分泌产生的在机体炎症反应中起作用的细胞因子。与其他炎症因子的区别...     

银屑病皮肤白细胞介素8的免疫组织化学研究

应用抗白细胞介素8(IL—8)的单克隆和多克隆抗体对IL—8在银屑病皮肤的定位与分布进行了免疫组织化学研究.进行期寻常型银屑病患者和健康对照各7例,分别切取病损处及其周围未受累皮肤和正常人皮肤进行冰冻切片.用IL—8抗血清和两种IL—8单抗(5A4、2G2)以ABC免疫组化技术染色观察结果.发现IL—8抗血清和单抗5A4能使正常人表皮着色,且基底细胞层明显深染.5A4、2G2在银屑病皮损表皮的基底以上层呈现阳性,而未受累皮肤的染色结果无明显规律性,银屑病皮损表皮与正常人皮肤IL—8的分布差异表明IL—8在该病的病理生理过程中可能发挥重要作用.     

特发性肺间质纤维化的HRCT诊断

特发性肺间质纤维化（idiopathic intersitial pulmonary fibrosis以下简称IPF）系下呼吸道原因不明的慢性炎症性疾病，它以侵犯肺泡壁和肺间质为特征的慢性炎症，参与炎症反应的以吞噬细胞和中性粒细胞为主，尚有其他各种类型的细胞，产生纤维细胞增殖和胶元纤维的堆积。IPF临床症状缺乏特异性，影像上亦缺乏特异性，影像表现复杂，有些表现与其他肺部疾病的影像表现有相似之处，HRCT可以更好的显示病变，对于本病的诊断和鉴别诊断有重要作用，本文对35例IPF的HRCT表现进行回顾性分析，将结果报告如下。     

细胞因子白介素-6和白介素-10对中性粒细胞凋亡的影响

 目的观察白介素-6(IL-6)和白介素-10(IL-10)对离体健康人中性粒细胞凋亡的影响,了解它们在全身炎症反应综合征(Systemic inflammatory response syndrome,SIRS)和多器官功能障碍综合征(Multiple organ dysfunction syndrome,MODS)发病中的作用.方法选择20例健康志愿者,采取外周静脉血并分离中性粒细胞,每例分离的中性粒细胞平均分为4等份,分别给予生理盐水(对照组)、IL-6、IL-10、IL-6+IL-10,孵育24 h后,用AO/EB染色法计算每个样本中中性粒细胞的凋亡率、坏死率,并比较各组间凋亡率、坏死率的差异.结果与刘照组相比,IL-6组的中性粒细胞凋亡率显著降低(P＜0.01),L-10组中性粒细胞凋亡率则没有显著差异(P＞0.05);IL-10可对抗IL-6抑制中性粒细胞凋亡的作用(IL-6+IL-10组47.95±9.83 VS IL-6组35.80±8.59,P＜0.01).与对照组相比,IL-6组的中性粒细胞坏死率显著增加(P＜0.01),而IL-10组中性粒细胞坏死率则没有显著差异(P＞0.05).结论IL-6可抑制中性粒细胞的凋亡,增加中性粒细胞的坏死,促进中性粒细胞参与的炎症反应.IL-10不影响正常中性粒细胞的功能,但可抑制激活的中性粒细胞参与的炎症反应,具有一定的免疫调节作用.     

白细胞介素8水平检测诊断难治性肺炎支原体肺炎价值研究

目的:探讨白细胞介素8(IL-8)水平检测诊断难治性肺炎支原体肺炎的临床价值。方法:选择难治性肺炎支原体肺炎40例为观察组,同期在本院儿科门诊体检健康儿童40例为对照组。采用酶联免疫吸附法(ELISA)测定两组IL-8水平,并比较两组IL-8水平的差异。结果:观察组急性期IL-8水平非常显著高于对照组(P<0.01);恢复期与对照组比较,差异不显著(P>0.05)。结论:IL-8水平检测对难治性肺炎支原体肺炎具有较高的临床诊断和预测价值。     

人重组白细胞介素-8(rhIL-8)对受照小鼠抗放作用的实验研究

目的：研究rhIL-8对放射损伤小鼠的保护作用及其机理。方法：在小鼠受照前不同时间予腹腔注射rhIL-8 10μg或30μg,观察动物的存活情况及一些指标。结果：小鼠受照后的存活时间与rhIL-8的给药剂量及时间密切相关。通过检测受照后小鼠的骨髓细胞CUF－GM计数、血清总SOD活性MDA含量以及一些免疫功能指标,提示它们与动物的存活时间有内在联系,结论rhIL-8具有一定的抗放作用,其机理可能与造血调控、免疫调控、清除自由基等多方面有关。     

高温作训环境对战士外周血IL-17及中性粒细胞和淋巴细胞变化的影响

目的：观测高温环境对执行训练任务战士外周血IL-17浓度及中性粒细胞和淋巴细胞含量的影响。方法选取健康训练战士160名,随机分为两组,每组80人,分别于（32±1）℃（试验组）和（22±1）℃（对照组）环境中连续训练2 h后,抽取静脉血检测IL-17浓度及外周血中性粒细胞和淋巴细胞含量。结果在高温环境训练40、50、60 min战士的体温分别为（38．74±0．26）、（39．23±0．24）、（39．51±0．34）℃,较常温环境训练的战士明显增高,差异有统计学意义（P＜0．05）。在高温环境训练40、50、60 min战士的自感劳累等级评分分别为17．62±0．66、18．03±0．56、18．47±0．84,较常温环境训练的战士明显增高,差异有统计学意义（P＜0．05）。高温训练2 h后战士外周血中IL-17浓度为（7．12±4．03）pg／ml,外周血中性粒细胞为（6．43±1．27）×109／L,淋巴细胞为（9．84±1．36）×109／L,与对照组相比均有明显增高,差异有统计学意义（P＜0．05）。结论高温环境能明显增高训练战士体温、自感劳累等级评分、外周血中IL-17的浓度以及中性粒细胞和淋巴细胞数。     

Radiation-induced matrix production of lung fibroblasts is regulated by interleukin-8

Purpose:?Lung fibrosis can be caused by radiation therapy during cancer treatment and therefore can be the limiting factor of the treatment. The factors that cause the actual fibrosis and the interaction between different cell types were investigated.

Materials and methods:?Epithelial lung cells and fibroblasts were irradiated and different cytokines were measured in the supernatant. Also effects of radiation on the matrix production of fibroblasts were investigated.

Results:?Irradiation of isolated lung fibroblasts did not cause increased extracellular matrix production; however, the co-culturing of fibroblasts and irradiated lung epithelial cells or the treatment of fibroblasts with supernatants of irradiated epithelial cells did result in an increase. We were able to show that increased interleukin-8 (IL-8) levels led to increased matrix production.

Conclusions:?IL-8 is not only a proinflammatory cytokine but it also stimulates collagen synthesis and matrix production and therefore could be a possible drug target in preventing radiation damage during cancer therapy.     

Interobserver agreement of interstitial lung fibrosis Reporting and Data System (ILF-RADS) at high-resolution CT

Emergency Radiology - To assess the interobserver agreement of interstitial lung fibrosis Reporting and Data System (ILF-RADS) in interpretation and categorization of interstitial lung disease...     

Ventilation/perfusion mismatch in interstitial fibrosis

Regional pulmonary perfusion defects unmatched by ventilation loss, occurring in a patient with proven interstitial lung fibrosis, is reported. This condition is not a widely recognized cause of ventilation/perfusion mismatch.     

炎症介质在海水淹溺急性肺损伤发生机制中的作用

Increasing importance has been attached to seawater drowning induced acute lung injury (SWD-ALI) due to its rapid progression, complicated pathogenesis and ineffective therapy. Seawater aspiration caused by drowning can activate acute inflammatory response and release infla mmatory related cells and factors, which can perform cascade reaction leading to partial inflammation in lung or systemic inflammatory response syndrome. A variety of inflammatory mediators participate initiation and enhancement of inflammatory response such as cytokines including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, nuclear factor(NF)-κB, neutrophils, reactive oxygens, proteases and so on. In this paper, the effect of inflammatory mediators on pathogenesis of SWD-ALI is reviewed.     

卡托普利对急性肺损伤大鼠TNF-α和IL-8的影响

目的：探讨卡托普利对急性肺损伤（ALI）大鼠血清TNF-α和IL-8的影响。方法：36只成年Wistar大鼠随机平均分为正常对照组、烟雾致伤组（ALI组）和卡托普利干预组（CAP组）。CAP组腹腔注射卡托普利（5 mg/kg）,对照组和ALI组腹腔注射等量生理盐水。15 min后复制烟雾吸入性肺损伤模型。致伤后5 min、5 h采动脉血行血气分析,致伤后5 h检测血清TNF-α、IL-8浓度。结果：ALI组血清TNF-α、IL-8含量明显高于正常对照组（P〈0.01）。CAP组血清IL-8含量显著低于ALI组（P〈0.05）,动脉血气明显改善,TNF-α两者无显著差异（P〉0.05）。结论：卡托普利能降低ALI大鼠血清IL-8浓度,对TNF-α无显著影响。     

CT features of lung disease in patients with systemic sclerosis: comparison with idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia

PURPOSE: To evaluate computed tomographic (CT) patterns of lung disease in patients with systemic sclerosis (SSc) and compare them with CT appearance in patients with biopsy-proved idiopathic pulmonary fibrosis (IPF) and idiopathic nonspecific interstitial pneumonia (NSIP). MATERIALS AND METHODS: The CT features of consecutive patients with SSc (n = 225; male patients, 44; female patients, 181; median age, 47 years; age range, 16-78 years), IPF (n = 40; men, 26; women, 14; median age, 54.5 years; age range, 36-77 years) and NSIP (n = 27; men, 18; women, nine; median age, 53 years; age range, 32-68 years) were quantified separately by two observers. The extent of interstitial lung disease, ground-glass opacification, emphysema, and the coarseness of a reticular pattern were quantified. Group comparisons were made nonparametrically with the Wilcoxon rank sum test. Differences in CT features were identified with multiple logistic regression analysis. RESULTS: The coarseness of fibrosis was similar in patients with SSc and idiopathic NSIP but strikingly different between patients with SSc (median coarseness score, 5.5; range, 0.0-13.3) and IPF (median coarseness score, 8.8; range, 2.5-15.0) (P <.001). The proportion of ground-glass opacification at CT was similar in patients with SSc and idiopathic NSIP but differed significantly between patients with SSc (median proportion, 49.9%; range, 0.0%-100.0%) and IPF (median proportion, 23.5%; range, 0.0%-97.2%) (P <.001). At logistic regression analysis, there were no differences in the CT features between patients with SSc and those with NSIP after controlling for age, disease extent, and the percentage predicted forced vital capacity and carbon monoxide diffusing capacity. CONCLUSION: Interstitial lung disease in patients with SSc is less extensive, less coarse, and characterized by a greater proportion of ground-glass opacification than that in patients with IPF. The CT features of lung disease in patients with SSc closely resemble those in patients with idiopathic NSIP.     

Wnt信号通路在肺间质纤维化中的研究进展

Wnt信号通路是参与健康组织和器官生长发育的关键,该通路的异常激活会导致纤维化、肿瘤等疾病的发生,这在不同的组织和器官中具有普遍性;临床前研究表明与Wnt相关器官纤维化的机制和治疗取得一定的进展,但缺乏临床数据支持,患者的生存质量并没有显著的提高。本文就Wnt信号通路与肺间质纤维化关系综述,其中特发性肺纤维化的研究相对成熟,继发性肺间质纤维化中重点介绍新型冠状病毒肺炎与Wnt信号通路的关系。