EFFECTS OF β-ENDORPHIN ON PHYTOHEMAGGLUTININ -INDUCED LYMPHOCYTE PROLIFERATION AND MOUSE PLAQUE-FORMINGCELL RESPONSE VIA AN OPIOID RECEPTOR MECHANISM

The effects of opioid peptides on immune responses were investigated.It was found that β-endorphin(β-END) can depress proliferative responses to PHA in rat splenocytes but enhance those in mice,and it could also inibit the plaque-forming cell(PFC) response to sheep red blood cells when mouse splenocytes immunized in vivo were cultured in vitro with the peptide.The peptide antagonist naloxone was able to reverse β-END suppression of the PFC response.The data indicate that β-END suppresses antibody production or secretion via a specific opioidreceptor-mediated mechanism.     

EFFECTS OF MTX AND BN_52021 ON PAF－INDUCED CHEMOTAXIS OF PMNs AND I

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EFFECTS OF NALOXONE AND AMINOPHYLLINE ON THE ANTINOCICEPTION INDUCED BY SEROTONANDNOREPINEPHRINEATTHESPINALLE

ＥＦＦＥＣＴＳＯＦＮＡＬＯＸＯＮＥＡＮＤＡＭＩＮＯＰＨＹＬＬＩＮＥＯＮＴＨＥＡＮＴＩＮＯＣＩＣＥＰＴＩＯＮＩＮＤＵＣＥＤＢＹＳＥＲＯＴＯＩＮＡＮＤＮＯＲＥＰＩＮＥＰＨＲＩＮＥＡＴＴＨＥＳＰＩＮＡＬＬＥＶＥＬ：ＡＳＴＵＤＹＵＳＩＮＧＥＭＧＰＬＡＮＩＭＥ...     

EFFECTS OF ALFENTANIL AND ESMOLOL ON HEMODYNAMIC AND CATECHOLMINE RESPONSE TO TRACHEAL INTUBATION

Objective.To compare the effects of alfentanil and esmolol on hemodynamic and catecholamine response to tracheal intubation.Methods.hiry-five adult patients were randomly allocated to one of three groups,Grup A(control group),Group B(esmolol group)and Group C(alfentanil group).The patients received either 2 mg/kg esmolol(inGroup B)or 30 μg/kg alfentanil(in GroupC)before intubation.Tracheal intubation was performed with 4 mg/kg thiopental and 0.1 mg/kg vecuronium and 3% isoflurane.Systolic blood pressure(SBP),diastolic blood pressure(BP),mean blood pressure(MBP),heart rate(HR),norepinephrine(NE),epinephrine(E)and dopamine(DA)were measured before and after intubation.Results.The control group had a baeline SBP of 149±23 mmHg while Groups B,C had a baseline SBP of 148±23,and 150±21mmHg,respectively(P＞0.05).Three min after tracheal intubation,the control group SBP increased to 160±30 mmHg and Group B remained at the baseline level,14±5 mmHg,and Group C significantly decreased to 91±22 mmHg(P＜0.01).Two min after intubation HR in Group B increased significantly but 3 min after intubation HR in Groups B and C were significantly lower than that of contrl group(P＜0.05).NE in Groups A and B increased significantly to 5.75±3.51 and 6.75±3.30 nmol/L 3 min after intubation(P＜0.01).In Group C,3 min after intubation NE was not significantly differnt from the basline but E decreased significantly(P＜0.01).Conclusion.2 ?g/kg esmolol can moerate the hemodynamic response to tracheal intubation to a certain extent and 30μg/kg alfentanil can completely attenuate the hemodynamic and catecholamine responses.     

EFFECTS OF RAPAMYCIN ON INTRACELLULAR CHOLESTEROL HOMEOSTASIS OF GLOMERULAR MESANGIAL CELL IN THE PRESENCE OF INTERLEUKIN-1β

Objective To investigate the effects of rapamycin on cholesterol homeostasis of glomerular mesangial cells and the underlying mechanisms.
Methods Intracellular cholesterol accumulation was measured by Oil Red O staining and high performance liquid chromatography. The effects of rapamycin on interleukin-1β（1L-1β）-induced mRNA and protein changes of low-density lipoprotein receptor （LDLR） and ATP-binding cassette transporter Al （ABCAl） were assayed by quantitative real-time PCR and Western blot. Transient expressions of 3 types of mammalian target of rapamycin （mTOR）, including mTOR-WT （wild type）, mTOR-RR （rapamycin resistant, with kinase activity）, and mTOR-RR-KD （rapamycin resistant, without kinase activity）, were obtained by plasmid transfection.
Results Rapamycin had no significant influence on intracellular cholesterol concentration trader normal condition, but it significantly decreased the intracellular cholesterol concentration in the presence of IL-1β. Rapamycin dose-dependently suppressed the increased expression of LDLR induced by IL-1β and up-regulated the suppressed expression of ABCAl caused by IL-1β Transient expression of 3 types of mTOR all reduced ABCAl mRNA expression significantly, which all could be overroded by rapamycin.
Conclusions Rapamycin may contribute to the maintaining of glomerular mesangial cell intracellular cholesterol homeostasis under inflammatory state by both reducing cholesterol uptake and increasing cholesterol effiux. And the effect may be not completely mediiated by mTOR.     

THE EFFECTS OF DEPRENYL AND 1-METHYL-4-PHENYL-1, 2, 3, 6-TETRAHYDROPYRIDINE (MPTP) ON 2-DEOXYGLUCOSE UPTAKE IN THE MOUSE BRAIN

~3H-2-deoxyglucose (2-DG) autoradiographic technique was used to study the ef feets of a monoamine-oxidase-B (MAO-B) inhibitor deprenyl and the neurotoxin Ⅰ-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) on 2-DG uptake in the mouse brain. Following MPTP intoxication, 2-DG uptake was increased in the substantia nigra and lo(?)us ceruleus. At the same time, obvious abnormal behavior of the animals was induced. In the mice pretreated with deprenyl, 2-DG uptake was similar to that of control animal. Ab normal behavior. though present, was significantly milder than in mice given MPTP alone. It is concluded that MPTP interferes with the glucose metabolism in the substantia nigra and locus ceruleus and induces remarkable abnormal behavioral syndrome of mice. These deleterious effects can be blocked by pretreatment with deprenyl.