高压氧治疗高血压性脑出血疗效分析

目的评价高压氧治疗高血压性脑出血的疗效。方法100例行高压氧治疗的高血压性脑出血患者为治疗组，同期未行高压氧治疗的高血压性脑出血患者为对照组，对比其显效率。结果治疗组总显效率为61％，对照组显效率为42％（X^2=4．86，P〈0．05）。结论高压氧治疗可提高高血压性脑出血患者的神经功能恢复。     

运动与高压氧疗法对骨质疏松症的治疗作用

人口老龄化和骨质疏松的发生率呈明显正相关性。治疗骨质疏松的途径很多,除药物防治外,运动锻炼、高压氧疗对防治骨质疏松有一定疗效。综合国内外文献报道,文内简述了运动对老年性骨质疏松症的防治作用,从运动对骨密度的影响、对骨的改变和调节作用、使成骨细胞活性升高、促进性激素分泌、促进钙吸收和利用等方面进行了分析。     

Effects of carbon monoxide poisoning on neutrophil responses in patients treated with hyperbaric oxygen.

BACKGROUND: Carbon monoxide (CO) poisoning can cause tissue injury. Neutrophil granulocytes have been proposed to contribute to the injury, which may be ameliorated by hyperbaric oxygen (HBO2) treatment. We sought to assess the relationship between acute CO poisoning and blood neutrophil count, plasma cytokine, and cortisol responses, as well as the mechanism behind the observed beneficiary effects of HBO2 treatment. METHODS: Eight patients (age 26-82 years) with severe acute CO poisoning were enrolled, concomitant with eight healthy controls (age 27-42 years), in a prospective, controlled, clinical study. The patients were given three HBO2 treatments (2.8 atmospheres absolute, 100 minutes) within the first 24 hours. The controls were given identical simultaneous HBO2 treatments. Venous blood samples were taken before and after each treatment. Results: At the start of the HBO2 treatment, patients displayed significantly higher blood neutrophil counts (p < .0001) and plasma cortisol levels (p = .020) than controls, but the two groups had similar values for interleukin-8, granulocyte colony-stimulating factor (G-CSF), neutrophil H2O2 generation, and CD16 and CD18 surface expression. During the observation time, neutrophil H2O2 accumulation declined in patients and in controls (p = .031), whereas the up-regulation of CD18 expression increased (p = .002) in both groups. Moreover, G-CSF levels became significantly higher in patients than in controls (p = .015). G-CSF levels also correlated significantly with neutrophil counts. Conclusion: CO poisoning was associated with discrete changes of blood neutrophil counts, cortisol, and G-CSF plasma concentrations. HBO2 treatment modulated neutrophil generation of H2O2 and surface expression of CD18. These changes may be part of the cascade of events leading to the sequelae of CO poisoning and their attenuation by HBO2.     

高压氧对大鼠急性胰腺炎影响的研究

目的 探讨高压氧对大鼠急性胰腺炎的影响.方法 56只SD大鼠随机分组,其中48只分为对照组和高压氧干预组,每组24只,均采用胰管结扎的方法制备胰腺炎模型,造模后每组再随机分为3亚组:每亚组分别在造模后1、3、7 d处死,仅对高压氧治疗组进行高压氧干预.两组均留取胰腺标本及血液使用病理评分评价胰腺标本出血、坏死和水肿方面的差异,并同时使用ELISA法检测血液IL-2、IL-6、IL-10及TNF-α水平.剩余8只为假手术组仅进行开关腹用于验证胰腺炎造模是否成功.结果 造模后第一天胰腺淀粉酶水平在假手术组为(798.50±113.5)U/L,对照组为(3156.2±639.9)U/L,高压氧治疗组为(2973.1±1042.6)U/L,其中假手术组与对照组及高压氧组之间有统计学差异,但对照组及高压氧治疗组之间差异无统计学意义.经过7 d的高压氧治疗之后,胰腺炎大鼠的细胞因子IL-2、IL-6、IL-10及TNF-α浓度水平较对照组明显下降,差异有统计学意义,而在病理评价方面,对胰腺炎的水肿、白细胞浸润、坏死和出血等方面均有改善作用,但仅在出血和坏死方面差异有统计学意义.结论 高压氧治疗组可以显著减少大鼠胰腺腺泡的坏死和出血,但是对于水肿和白细胞浸润方面则并未得到预期的结果.同时高压氧治疗可以显著抑制血液炎症因子的活性.     

Effect of hyperbaric oxygen on endotoxin-induced lung injury in rats

Oxygen therapy remains the main component of the ventilation strategy for treatment of patients with acute lung injury. Hyperbaric oxygen therapy (HBO(2)) is the intermittent administration of 100% oxygen at pressure greater than sea level and has been applied widely to alleviate a variety of hypoxia-related tissue injuries. The purpose of this study was to evaluate the effect of hyperbaric oxygen on acute lung injury induced by intratracheal spraying of lipopolysaccharide (LPS) in rats. Male Sprague-Dawley rats underwent implantation of a carotid artery catheter under general anesthesia. Aerosolized LPS was delivered twice into the lungs via intratracheal puncture. Animals were either breathing room air (n = 27) or subjected to hyperbaric oxygen (HBO(2)) exposure (n = 27) 1 h after LPS spraying. Acute lung injury was evaluated 5 h and 24 h later. Compared with the control group, intratracheal spraying of LPS caused profound hypoxemia, greater wet/dry weight ratio (W/D) of the lung (5.67 +/- 0.22 vs. 4.98 +/- 0.19), and higher protein concentration (1706 +/- 168 vs. 200 +/- 90 mg/L) and LDH activity (129 +/- 30 vs. 46 +/- 15, mAbs/min) in bronchoalveolar lavage (BAL) fluid. Intratracheal spraying of LPS also caused significant WBC sequestration in the lung tissue. HBO2 treatment significantly reverted hypoxemia, reduced lung injury measures evaluated at 5 and 24 h, and enhanced 24-h animal survival rate (chi = 5.08, P = 0.024). The malondialdehyde (MDA) concentrations in lung tissue and serum were both increased after LPS spraying. Neither single HBO(2) therapy nor five sequential daily treatments enhanced MDA production in lung tissue or serum. Our results suggested that hyperbaric oxygen might reduce acute lung injury caused by intratracheal spraying of LPS in rats. This treatment modality is not associated with enhancement of oxidative stress to the lung.     

高压氧对皮肤慢性溃疡的促愈作用

INTRODUCTION At present, treatment of chronic ulcer of skin in clinic is very diffi-cult. Effect of comprehensive treatment by hyperbaric oxygen onchronic ulcer is obvious.     

高压氧对人胶质瘤172细胞生存的影响

目的:观察高压氧(HBO)对人胶质瘤172细胞生存的影响。方法:常规培养的人胶质瘤172细胞随机分为对照组和HBO组,对照组每日实验舱中常压0.1MPa空气放置90min,其余时间正常培养,HBO组每日HBO暴露1次,将细胞置实验舱中,氧气加压,15min升压至0.2MPa,稳压1h,减压15min,其余时间培养同对照组,HBO暴露10次。第11d取材,观察2组细胞生长情况,流式细胞仪检测细胞凋亡比例、细胞生长周期,电镜观察细胞超微结构。结果:HBO组细胞计数明显少于对照组(P=0.01),HBO组早期和晚期凋亡细胞比例均明显高于对照组(均P<0.01)。2组G1期和S期细胞比例比较差异均无统计学意义,HBO组G2期细胞比例较对照组明显减少(P<0.01)。电镜观察到HBO组肿瘤细胞较对照组突起短小、减少。结论:HBO减慢了人胶质瘤172细胞生长,促进细胞凋亡,使更多的细胞阻滞在S期,使与肿瘤的转移、复发有关的突起变短、减少,显示了HBO对肿瘤细胞的抑制作用。     

高压氧预处理对缺血-再灌注诱导糖尿病大鼠心肌细胞凋亡的影响

目的 探讨高压氧预处理对糖尿病大鼠心肌细胞凋亡的影响.方法 雄性Wistar大鼠45只,糖尿病造模成功后随机分为糖尿病对照组、糖尿病缺血-再灌注组、糖尿病高压氧预处理组,检测心肌凋亡及心肌Bax、Bcl-2蛋白表达.结果 与糖尿病缺血-再灌注组比较,糖尿病高压氧预处理组凋亡指数、Bcl-2蛋白灰度显著降低[(52.73±6.71)%vs(41.69±5.79)%,(183.33±9.15)vs(166.00±10.53),P<0.05],Bax蛋白灰度显著升高[(134.00±4.73)vs(141.17±6.77),P<0.05].结论 高压氧预处理有减少糖尿病大鼠心肌细胞凋亡的作用,其机制可能与下调Bax蛋白的表达,上调Bcl-2蛋白表达有关.     

Effect of hyperbaric oxygen on flow-mediated vasodilation: an ultrasound study

OBJECTIVE: The purpose of this study was to investigate the effect of hyperbaric oxygen (HBO) treatment on flow-mediated vasodilation (FMD) by ultrasound examination. METHODS: We studied 14 young patients without cardiovascular problems who underwent HBO treatment. The indications for HBO treatment were osteomyelitis (n = 8), Crohn disease (n = 2), perianal abscesses (n = 2), lingual artery embolization (n = 1), and aseptic necrosis (n = 1). The ultrasound evaluation for FMD was performed before HBO treatment, after 1 session of HBO treatment, and after 10 sessions of HBO treatment. The right brachial artery FMD response was evaluated by the mean of the baseline right brachial artery diameter, absolute change in the diameter before and after cuff inflation/deflation, and percent change in the diameter. RESULTS: Statistical analysis showed a significant change in the preinflation right brachial artery diameter before (mean +/- SD, 3.6 +/- 0.54 mm) and after (3.76 +/- 0.56 mm) 10 sessions of HBO treatment (P < .05). The absolute changes in the right brachial artery diameter before and after cuff inflation/deflation (0.36 +/- 0.2 mm before HBO treatment, 0.37 +/- 0.22 mm after 1 session of HBO treatment, and 0.38 +/- 0.21 mm after 10 sessions) and percent change in FMD (10% +/- 5.8% before HBO treatment, 10.6% +/- 7.5% after 1 session of HBO treatment, and 10.6% +/- 7.7% after 10 sessions) after induction of a hyperemic response by cuff inflation were not statistically significant (P > .05). CONCLUSIONS: Hyperbaric oxygen treatment did not have an immediate effect on FMD (absolute change in the right brachial artery diameter after cuff inflation/deflation); however, the right brachial artery diameter increased after 10 sessions of HBO treatment. This may suggest chronic stress on the vascular endothelium after HBO.     

高压氧对糖尿病患者周围神经传导速度的影响

目的:探讨高压氧治疗对糖尿病并发周围神经病患者周围神经传导速度(nerveconductionvelocity,NCV)的影响,并将运动神经和感觉神经的结果予以分析比较。方法:糖尿病患者69例随机分为两组,对照组31例采用糖尿病饮食、降血糖、能量合剂、促代谢药物等治疗,治疗组38例在上述治疗的基础上加用高压氧治疗。采用多人高压氧舱,在舱内停留100min,压力0.20MPa,戴面罩吸入纯氧60min,中间休息10min,1次/d,10d为1个疗程,共3个疗程,疗程间隔2～4d。分别于治疗前、治疗2个月后测定正中神经、尺神经、胫神经的NCV并进行比较。结果:治疗组治疗前后正中神经的运动神经传导速度(MNCV)分别为(40.9±5.6)m/s,(43.9±4.7)m/s,差异有非常显著性意义(P<0.01)、感觉神经传导速度(SNCV)分别为(41.2±4.9)m/s,(43.4±5.2)m/s,差异有非常显著性意义(P<0.01),尺神经MNCV分别为(42.3±4.1)m/s,(45.5±7.4)m/s,差异有非常显著性意义(P<0.01),SNCV分别为(40.6±5.3)m/s,(42.2±4.7)m/s,差异有显著性意义(P<0.05),胫神经MNCV分别为(41.9±7.8)m/s,(43.5±5.1)m/s,差异有显著性意义(P<0.05),SNCV分别为(39.7±5.4)m/s,(41.9±4.8)m/s,差异有非常显著性意义(P<0.01);对照组治疗后仅正中神经的MNCV、尺神经的MNCV显著加快;治疗前组间比较差异     

Effects of hyperbaric oxygen treatment on liver functions, oxidative status and histology in septic rats

Objective The liver is thought to be responsible for multiple organ failure during sepsis. Increase in tissue oxygen consumption is a major component of the septic response. Hyperbaric oxygen (HBO) therapy provides more oxygenation in the whole body. This study examined the effect of HBO alone or in combination with cefepime (CEF) on the liver in septic rats.Design and interventions We divided 90 male rats into six groups; control, HBO, sepsis (SEP), SEP+HBO, SEP+CEF, and SEP+CEF+HBO. Sepsis was induced with an intraperitoneal injection of Escherichia coli (2.1×10⁹ cfu). A total of six HBO sessions were performed at 2 atm absolute for 90 min at 6-h intervals. CEF was administered intraperitoneally at a dose of 50 mg/kg twice daily. Animals were killed 48 h after sepsis induction. Their liver and blood were removed for biochemical and histopathological analysis.Measurements and results Liver thiobarbituric acid reactive substances as well as serum alanine transaminase, aspartate transaminase and alkaline phosphatase levels increased while the activity of the antioxidant enzymes superoxide dismutase and catalase decreased significantly in septic rats. These parameters returned to nearly control levels in the SEP+CEF+HBO group. Histological observations supported these findings: Hepatocellular degeneration was observed and intensive polymorphonuclear cell infiltration appeared in all fields of septic animal livers. HBO alone could not sufficiently reverse these histopathological changes, but most liver sections presented normal histology when it was combined with CEF.Conclusions HBO may be a useful adjuvant therapy modality to improve the efficacy of sepsis treatment.This article refers to the editorial     

人工鼻吸氧对多人高压氧舱内氧浓度的影响

目的:在多人氧舱内高压氧治疗过程中气管切开昏迷患者给予人工鼻吸氧,观察人工鼻吸氧方式对舱内氧浓度、理论通风换气量和实际压缩空气使用量的影响。方法:选择2000-08/2005-08暨南大学第一、二临床医学院高压氧科收治的行高压氧治疗的40例气管切开昏迷患者,另选择440例行高压氧治疗的清醒患者,两类患者在年龄、原发病方面比较差异无显著性意义(P>0.05)。本实验操作过程均为常规临床技术操作,遵循国家所制定的伦理学标准,所有患者及家属都被告知实验目的并同意参加。①高压氧治疗方案:0.20MPa,30min×2 10min,1次/d,10d/疗程,间歇吸氧。②高压氧吸氧方式:按国标要求440例清醒患者采用常规面罩和呼吸回路连接吸氧。而40例气管切开昏迷患者采用如下吸氧方式:人工鼻吸氧(20例):将人工鼻置于人工气道外接口和吸排氧回路的“Y”型管之间,连接时动作轻柔,检查人工鼻连接的密封。护理人员用手轻扶人工鼻,防止人工鼻重力压迫、刺激气管;局部面罩一级供氧吸氧(10例):将面罩罩于气管切开外套管处,局部一级持续供氧、舱内排氧。传统头盔吸氧(10例):一级持续供氧、氧舱内排氧。舱内吸氧休息阶段,摘除带有“Y”型管的吸氧回路,人工鼻保留在气管外套管上;其他吸氧方式完全移开吸氧回路,停止吸氧和一级供氧,直接呼吸舱内压缩空气。③分组:每舱吸氧总人数均为12例,气管切开昏迷患者与常规面罩吸氧患者同舱治疗,分为4组:人工鼻 常规面罩吸氧组:人工鼻吸氧患者2例,常规面罩吸氧患者10例;局部面罩 常规面罩吸氧组:局部面罩一级供氧吸氧患者1例,常规面罩吸氧患者11例;传统头盔 常规面罩吸氧组:传统头盔吸氧患者1例,常规面罩吸氧患者11例;常规面罩吸氧组:常规面罩吸氧患者12例。④随机选取每组10个舱次的氧浓度记录和与之相应的压缩空气使用量,观察各组高压氧治疗过程氧舱内氧浓度、氧舱内理论通风换气量和实际压缩空气使用量的变化。采用SPSS12.0统计软件行卡方检验、t检验以及方差分析的LSD检验。结果:①各组氧舱内氧浓度的变化:人工鼻 常规面罩吸氧组、常规面罩吸氧组舱内平均氧浓度分别为(21.98±0.26)%和(21.95±0.22)%,均小于国标规定界限23%,两组比较基本相似(P>0.05)。局部面罩 常规面罩吸氧组、传统头盔 常规面罩吸氧组舱内平均氧浓度分别为(24.82±0.31)%和(25.12±0.41)%,均大于国标规定界限23%,两组比较基本相似(P>0.05)。②各组氧舱内氧浓度监测图的变化:人工鼻 常规面罩吸氧组、常规面罩吸氧组氧舱内氧浓度虽随着吸氧时间的延长而增加,但至吸氧结束时舱内氧浓度最高值分别为22.1%和21.9%,均低于23%,两组舱内氧浓度曲线相同。局部面罩 常规面罩吸氧组、传统头盔 常规面罩吸氧组随吸氧时间的延长氧舱内氧浓度明显增高,至吸氧结束时分别达到23.8%和24.1%,均超过23%,两组舱内氧浓度曲线相同。③各组高压氧治疗压缩空气实际用气量及理论通气量的比较:人工鼻 常规面罩吸氧组、常规面罩吸氧组高压氧治疗压缩空气实际用气量及理论通气量均明显少于局部面罩 常规面罩吸氧组、传统头盔 常规面罩吸氧组(P<0.01)。结论:气管切开昏迷患者在多人舱内使用人工鼻吸氧,氧舱内氧浓度<国标规定界限23%,且高压氧治疗期间理论通风量和压缩空气的使用量少。不仅保证了高压氧治疗的安全,同时节省了高压氧的成本消耗。     

人工鼻吸氧对多人高压氧舱内氧浓度的影响

目的：在多人氧舱内高压氧治疗过程中气管切开昏迷患者给予人工鼻吸氧，观察人工鼻吸氧方式对舱内氧浓度．理论通风换气量和实际压缩空气使用量的影响。 方法：选择2000-08／2005-08暨南大学第一、二临床医学院高压氧科收治的行高压氧治疗的40例气管切开昏迷患者，另选择440例行高压氧治疗的清醒患者，两类患者在年龄、原发病方面比较差异无显著性意义（P〉0．05）。本实验操作过程均为常规临床技术操作，遵循国家所制定的伦理学标准，所有患者及家属都被告知实验目的并同意参加。①高压氧治疗方案：0．20MPa，30min&;#215;2＋10min，1次／d，10d／疗程，间歇吸氧。②高压氧吸氧方式：按国标要求440例清醒患者采用常规面罩和呼吸回路连接吸氧。而40例气管切开昏迷患者采用如下吸氧方式：人工鼻吸氧（20例）：将人工鼻置于人工气道外接口和吸排氧回路的“Y”型管之间，连接时动作轻柔，检查人工鼻连接的密封。护理人员用手轻扶人工鼻，防止人工鼻重力压迫、刺激气管；局部面罩一级供氧吸氧（10例）：将面罩罩于气管切开外套管处，局部一级持续供氧、舱内排氧。传统头盔吸氧（10例）：一级持续供氧、氧舱内排氧。舱内吸氧休息阶段，摘除带有“Y”型管的吸氧回路，人工鼻保留在气管外套管上；其他吸氧方式完全移开吸氧回路。停止吸氧和一级供氧，直接呼吸舱内压缩空气。③分组：每舱吸氧总人数均为12例，气管切开昏迷患者与常规面罩吸氧患者同舱治疗，分为4组：人工鼻＋常规面罩吸氧组：人工鼻吸氧患者2例。常规面罩吸氧患者10例；局部面罩＋常规面罩吸氧组：局部面罩一级供氧吸氧患者1例，常规面罩吸氧患者11例；传统头盔＋常规面罩吸氧组：传统头盔吸氧患者1例，常规面罩吸氧患者11例；常规面罩吸氧组：常规面罩吸氧患者12例。④随机选取每组10个舱次的氧浓度记录和与之相应的压缩空气使用量，观察各组高压氧治疗过程氧舱内氧浓度、氧舱内理论通风换气量和实际压缩空气使用量的变化。采用SPSS12．0统计软件行卡方检验、t检验以及方差分析的LSD检验。 结果：①各组氧舱内氧浓度的变化：人工鼻＋常规面罩吸氧组、常规面罩吸氧组舱内平均氧浓度分别为（21．98&;#177;0．26）％和（21．95&;#177;0.22）％，均小于国标规定界限23％，两组比较基本相似（P〉0.05）。局部面罩＋常规面罩吸氧组、传统头盔＋常规面罩吸氧组舱内平均氧浓度分别为（24.82&;#177;0.31）％和（25．12&;#177;0．41）％，均大于国标规定界限23％，两组比较基本相似（P〉0.05）。②各组氧舱内氧浓度监测图的变化：人工鼻＋常规面罩吸氧组、常规面罩吸氧组氧舱内氧浓度虽随着吸氧时间的延长而增加，但至吸氧结束时舱内氧浓度最高值分别为22．1％和21．9％，均低于23％，两组舱内氧浓度曲线相同。局部面罩＋常规面罩吸氧组、传统头盔＋常规面罩吸氧组随吸氧时间的延长氧舱内氧浓度明显增高，至吸氧结束时分别达到23．8％和24．1％，均超过23％，两组舱内氧浓度曲线相同。③各组高压氧治疗压缩空气实际用气量及理论通气量的比较：人工鼻＋常规面罩吸氧组、常规面罩吸氧组高压氧治疗压缩空气实际用气量及理论通气量均明显少于局部面罩＋常规面罩吸氧组、传统头盔＋常规面罩吸氧组（P〈0.01）。 结论：气管切开昏迷患者在多人舱内使用人工鼻吸氧，氧舱内氧浓度〈国标规定界限23％，且高压氧治疗期间理论通风量和压缩空气的使用量少。不仅保证了高压氧治疗的安全，同时节省了高压氧的成本消耗。     

高压氧对脑外伤后一氧化氮及脑水肿的影响

观察高压氧对脑外伤后脑水肿及一氧化氮的影响。方法：建立大鼠液压脑外伤模型，采用生化、放免等方法观察脑外伤后不同时期高压氧（ＨＢＯ）治疗组和未治疗组血浆一氧化氮（ＮＯ）、脑组织ＮＯ、环磷酸鸟苷酸（ｃＧＭＰ）及脑组织含水量的变化，并进行病理学检查。结果：ＨＢＯ治疗组血浆ＮＯ、脑组织含水量、腋组织ＮＯ及ｃＧＭＰ较未治疗组均有不同程度下降（Ｐ＜０．０５）。脑组织含水量与血浆ＮＯ及脑组织ＮＯ、ｃＧＭＰ之间呈正相关关系（ｒ＝０．３４１４、０．３８１９、０．４８０４、Ｐ＜０．０５），病理检查可见ＨＢＯ治疗组脑组织损伤较轻。结论：ＨＢＯ能减轻脑外伤后脑水肿，并抑制脑外伤后ＮＯ的过量生成。ＨＢＯ减轻脑外伤后脑水肿的机制与ＮＯ有关。     

高压氧改善持续植物状态患者神志、语言及肢体功能观察

Background:The causes of persistant vegetative state (PVS) have varied forms. And there is no special therapy for PVS.The effects of PVS are varied either.It has been shown that hyperbaric oxygen has good effect in treating patients of PVS in their mentality, language and limb functions.     

高压氧对脑源性神经干细胞分化的影响

目的:探讨高压氧对缺血缺氧脑源性神经干细胞模型向神经元细胞分化的影响。方法:实验于2006-01/05在中南大学湘雅医院神经病学实验室完成。YLC0.5/1A型动物实验高压氧舱(湖北武汉)。①实验选取清洁级SD新生鼠10只,无菌条件下分离脑组织,克隆传代培养脑源性神经干细胞。将第3代细胞制成单细胞悬液,分装在不同的25mL培养瓶中,分为正常对照组、模型对照组、高浓度氧组、高气压组、高压氧组,1瓶/组。②除正常对照组外,其余各组均复制神经干细胞缺血缺氧模型。当培养的神经干细胞达80%融合时换为新鲜的完全培养基,次日将培养基弃去,换用新鲜的无血清DMEM/F12培养基,并将细胞置于体积分数为0.93的N2、0.05的CO2、0.02的O2培养箱中37℃培养,3h后用于相关指标检测。③从每组取1mL细胞悬液移入96孔酶标板,在酶联免疫检测仪490nm波长处测定各孔吸光度值,检测神经干细胞活力。④将各组神经干细胞悬液接种于盖玻片上涂有多聚赖氨酸的培养皿中,Nestin免疫荧光染色后加入去除生长因子的血清DMEM/F12培养基,各组在不同条件下继续培养:高气压组培养皿置于高压氧动物舱内,采用压缩空气给予0.2MPa压力1h(加、减压时间各15min,稳压30min),1次/d,连续7d;高压氧组在高气压组基础上,稳压时舱内氧浓度保持在80%以上;高浓度氧组不加压,只保持舱内氧浓度在80%以上;正常对照组和模型对照组在加入去除生长因子的血清DMEM/F12(含体积分数为0.2的胎牛血清)中培养7d进行诱导分化。各组神经干细胞进行特异免疫荧光染色,观察细胞分化情况。⑤采用显微镜在200倍视野下进行细胞计数,每组观察10张盖玻片,每张盖玻片观察6个不重复视野,计算各组神经干细胞分化为神经元及胶质细胞的百分率。结果:①造模后神经干细胞活力的观察:与正常对照组比较,模型对照组、高浓度氧组、高气压组、高压氧组神经干细胞复制缺血缺氧模型后吸光度值均明显下降(t=-4.357,P<0.05),表明神经干细胞经缺血缺氧后数目减少,生长活力下降。②脑源性神经干细胞诱导分化情况:诱导分化3d后,各组微管相关蛋白2免疫细胞化学染色可见神经元样细胞,胶质原纤维酸性蛋白免疫细胞化学染色可见星形胶质样细胞,半乳糖脑苷脂免疫细胞化学染色可见少突胶质样细胞。③各组神经干细胞分化结果比较:高压氧组神经元及胶质细胞分化率较正常对照组基本相似(t=0.324,P>0.05),但神经元分化率明显高于模型对照组、高浓度氧组、高气压组(t=2.667～5.424,P<0.05),胶质细胞分化率低于此3组(t=-5.424～-2.667,P<0.05)。结论:高压氧处理能够上调脑源性神经干细胞向神经元分化的比例。     

高压氧治疗脑外伤失语症60例疗效观察

目的观察高压氧(HBO)治疗脑外伤后失语症的治疗效果。方法将120例脑外伤患者随机分为HBO治疗组和常规组,常规组治疗采用临床常规治疗,HBO治疗组在常规治疗的基础上实施30次以上HBO治疗。在治疗前后分别用中国康复中心失语症检查法对语言功能进行评估和比较。结果 HBO治疗组和常规组显效率分别为63.3%、13.3%,HBO治疗组和常规组总有效率分别为93.3%、46.7%,差异有统计学意义(P0.05)。结论 HBO治疗脑外伤后失语疗效明显优于常规治疗组,能显著提高疗效。