Arylglycerol glucosides from Dracocephalum forrestii

Three new arylglycerol glucosides, threo-guaiacylglycerol 3-O-(6-O-p-hydroxybenzoyl)-beta-D-glucopyranoside (1), threo-guaiacylglycerol 3-O-[6-O-(E)-p-coumaroyl]-beta-D-glucopyranoside (2) and threo-guaiacylglycerol 3-O-[6-O-(Z)-p-coumaroyl]-beta-D-glucopyranoside (3), together with seven known compounds were isolated from the whole plants of Dracocephalum forrestii and their structures were determined on the basis of spectroscopic evidences.     

Two new iridal-type triterpenoids from Iris forrestii

Two new iridal-type triterpenoids, named forrestins A and B (1 and 2), were isolated from Iris forrestii Dykes by comprehensive chromatographic methods. The structures of 1 and 2 were elucidated by interpretation of extensive spectroscopic data including 1D and 2D NMR and HRESIMS.     

黑骨藤根挥发性成分研究

目的:研究黑骨藤根挥发性成分。方法:利用水蒸气蒸馏法提取黑骨藤根挥发性成分,用 GC-MS 进行分离测定,色谱柱为 SE-30弹性石英毛细管柱(30 m×0.25 mm×0.25μm),柱温50℃,保持2 min,然后以4℃·min~(-1)升温至230℃,保持5 min。载气为高纯度氦气99.999%;载气流量1.0 mL·min~(-1);分流比40:1。结合计算机检索技术对分离的化合物进行结构鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果:分离出26个成分,确定了21个化合物,占挥发油总量的95.61%。黑骨藤根挥发性成分大于1%的化学成分有5种,分别为:薁1.177%,α-雪松醇1.247%,芳樟醇1.248%,甲基萎叶酚2.297%,反式茴香脑83.013%。结论:本文对黑骨藤根挥发性成分的研究,为该类植物的进一步研究开发提供必要的试验数据。     

Hepatoprotective iridoid glucosides from Callicarpa nudiflora

Two new iridoid glucosides, callicoside A (1) and callicoside B (2), were isolated from the leaves of Callicarpa nudiflora. Their structures were elucidated by means of spectroscopic methods and chemical evidences. In an in vitro bioassay, compound 1 showed pronounced hepatoprotective activity against D-galactosamine-induced toxicity in WB-F344 rat hepatic epithelial stem-like cells.     

香青兰子化学成分的初步研究

目的研究香青兰(Dracocephalum moldavica L.)种子的化学成分。方法采用硅胶柱层析、Sephadex LH-20柱层析、薄层色谱、HPLC色谱等方法,分离纯化香青兰子的化学成分,通过UV、MS、NMR波谱方法对其结构进行解析和鉴定。结果分离并鉴定了6个化合物的结构,分别为田蓟苷(tilianin,1),β-胡萝卜苷(daucosterol,2),黄芪苷(astragalin,3),阿魏酸(ferulic acid,4);咖啡酸(caffeic acid,5),2,5-二羟基苯甲酸(2,5-dihydroxy benzoic acid,6)。结论所有化合物均为首次从香青兰子中分离得到。     

长柱重楼总皂苷体外抗肿瘤活性及毒性研究

目的研究长柱重楼总皂苷(PCT3)体外抗肿瘤活性及毒性。方法用改良二甲基四氮唑盐法检测PCT3、顺铂对人肺癌细胞株(A-549)和人肝癌细胞株(HEPG-2)的增殖抑制作用。小鼠一次性腹腔注射52,74,105,150mg·kg^-1PCT3,检测PCT3的半数致死量(LD50),并观察肝组织病理切片的变化,用比色法测定PCT3的溶血作用。结果 PCT3对A-549和HEPG-2细胞增殖抑制的半数抑制浓度(IC50)分别为11.71和2.36μg·mL^-1,顺铂对A-549和HEPG-2细胞增殖抑制的IC₅₀分别为6.31和5.33μg·mL^-1.PCT3对小鼠的LD₅₀为92.40 mg·kg-,其对动物的整体健康状态有一定的影响,具有一定的肝毒性。PCT3的溶血率随着浓度的增加而增加,其溶血的半数有效量为4.31μg·mL^-1。结论 PCT3对人癌细胞株A-549和HEPG-2细胞的增殖均有较强的抑制作用,对HEPG-2细胞的增殖抑制作用强于A-549细胞。同时,PCT3有一定的肝毒性和溶血活性,其溶血活性与细胞毒活性有较明显的相关性。     

Antioxidant phenolic glucosides from Gentiana piasezkii

An extract of Gentiana piasezkii afforded a new arbutin derivative 6′-O-vanilloylarbutin (1) and a new flavone-C-glucoside 7-O-feruloylorientin (2), together with four known flavonoids lutonarin (3), saponarin (4), isoorient (5) and luteolin (6). Their structures were established based on spectroscopic methods including 2D NMR (COSY and gHMBC) techniques. Compounds 1, 2, 5 and 6 were evaluated for the antioxidant activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay system.     

HPLC法同时测定苗药黑骨藤中6种咖啡酰基奎宁酸成分含量及指纹图谱研究

目的:建立HPLC法同时测定黑骨藤药材中6种咖啡酰基奎宁酸类成分的含量及液相指纹图谱,为其质量控制提供理论依据。方法:采用Agela Technologies Venusil XBP C₁₈色谱柱(250 mm×4. 6 mm,5μm),以乙腈-0. 1%磷酸水为流动相,梯度洗脱,流速为0. 8 m L·min^-1,检测波长为280 nm,柱温为25℃。结果:测定的6种成分5-O-咖啡酰基奎宁酸、3-O-咖啡酰基奎宁酸、4-O-咖啡酰基奎宁酸、3,4-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、4,5-O-二咖啡酰基奎宁酸分别在9. 84～252. 00,31. 63～506. 00,5. 76～147. 50,0. 62～15. 81,1. 45～37. 24,2. 94～75. 14 mg·L^-1范围内线性关系良好。平均加样回收率分别为102. 8%,91. 3%,92. 2%,97. 4%,92. 4%,97. 4%; RSD值分别为1. 6%,2. 1%,2. 6%,2. 1%,1. 9%,1. 0%。建...     

Antioxidant phenolic glucosides from Gentiana piasezkii

An extract of Gentiana piasezkii afforded a new arbutin derivative 6'-O-vanilloylarbutin (1) and a new flavone-C-glucoside 7-O-feruloylorientin (2), together with four known flavonoids lutonarin (3), saponarin (4), isoorient (5) and luteolin (6). Their structures were established based on spectroscopic methods including 2D NMR (COSY and gHMBC) techniques. Compounds 1, 2, 5 and 6 were evaluated for the antioxidant activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay system.     

苗药黑骨藤多糖部位HGT-5A的细胞免疫抑制作用及其可能的活性成分

目的观察苗药黑骨藤多糖部位HGT-5A的细胞免疫抑制作用及探讨其可能的活性成分。方法采用二硝基氯苯建立小鼠迟发型超敏反应模型,初次致敏当天开始ig给予HGT-5A,每天1次,连续10 d,第11天处死小鼠测定耳肿胀度,观察HGT-5A体内对细胞免疫反应的影响;用[3 H]TdR掺入法检测HGT-5A及其多糖组分对小鼠脾细胞增殖反应的影响;用MTT法检测HGT-5A对脾淋巴细胞存活的影响。结果HGT-5A 50和100 mg.kg-1可以明显抑制迟发型超敏反应模型小鼠耳肿胀,耳肿胀度由模型组的(8.9±2.2)mg分别降低至6.4±1.7和(7.1±1.5)mg;HGT-5A 50～500 mg.L-1体外应用可促进小鼠脾细胞自发增殖反应,但在HGT-5A 100 mg.L-1以上时可明显抑制刀豆蛋白A诱导的小鼠脾细胞增殖反应(P<0.05),对脂多糖诱导的小鼠脾细胞增殖反应无明显影响;HGT-5A与脾细胞共培养24,48和72 h对脾细胞存活无明显影响。从HGT-5A分离获得的中性糖部位HP1,酸性糖部位HP2,从HP1中分离得到的多糖成分HP1-3,以及从HP2中分离得到的多糖成分HP2-3和HP2-4 0.5～50 mg.L-1可促进脾细胞自发增殖反应;HP2,HP1-3,HP1-4,HP2-2和HP2-4明显抑制刀豆蛋白A诱导的T细胞增殖反应。结论 HGT-5A可抑制T细胞活化增殖,对细胞免疫反应具有抑制作用,多糖成分HP1-3,HP1-4,HP2-2和HP2-4可能是黑骨藤发挥免疫抑制作用的活性成分。     

Pervosides A and B,new isoferulyl glucosides from Perovskia atriplicifolia

Pervosides A (1) and B (2), two new isoferulyl glucosides, have been isolated from the ethyl acetate soluble fraction of Perovskia atriplicifolia and their structures have been assigned on the basis of spectroscopic data. 3-Hydroxyestragole β-d-glucopyranoside (3) has also been isolated and characterized for the first time from this species.     

藏药甘青青兰脂溶性化学成分的研究(Ⅰ)

目的研究甘青青兰(Dracocephalum tanguticum Maxi m.)的化学成分。方法采用硅胶柱色谱法分离化合物,运用波谱技术分析确定化学结构。结果与结论从甘青青兰的超临界提取物中共分离鉴定了5个化合物,经波谱分析分别确定为:齐墩果酸(1)、熊果酸(2)、正三十三烷烃(3)、白桦酯醇(4)、3β,20α-二羟基乌苏烷-21(22)-烯-28-酸(5)。化合物3为首次从该植物中分离得到,化合物5为首次从该属植物中分离得到。     

Three new flavane glucosides from the leaves of Morus wittiorum

Three new flavane glucosides (1–3) were isolated from the leaves of Morus wittiorum. The structures with absolute configuration were determined on the basis of hydrolysis and spectroscopic methods including UV, IR, HR-ESI-MS, 1D, and 2D NMR.     

Two new terpene glucosides and antitumor agents from Centipeda minima

One new monoterpene glucoside minimaoside A (1) and one new sesquiterpene glucoside minimaoside B (2), together with four known terpenoids, were isolated from the whole plants of Centipeda minima (L.) A. Braun et Ashers. Their structures were determined by spectroscopic methods. Compounds 5 and 6 showed weak or moderate cytotoxic activity toward several tumor cell lines.     

岩青兰的化学成分及药理学研究综述

唇形科青兰属植物岩青兰具有较高的药用价值,研究表明岩青兰中含有北美圣草素,木犀草素,柚皮素-7-O-β-D-葡萄糖苷等黄酮类化合物,熊果酸,白桦脂酸,间羟基苯甲酸等有机酸类,β-谷甾醇,白桦脂醇,正十八醇十八酸酯等挥发性油以及Ca、Mg、Fe等微量元素,具有血小板活化因子(PAF)拮抗,保护心肌,抗炎,抗癌等药理作用,临床应用广泛。本文就岩青兰的化学成分及其药理作用方面作一综述。     

Phenolic acid glucosides from the seeds of Entada phaseoloides Merill

Phytochemical investigation of the defatted seeds of Entada phaseoloides Merill. (Mimosaceae) led to the isolation of three new phenolic acid glucosides, which were characterized as 2-hydroxy-5-methylbenzoyl-β-l-glucopyranoside (p-cresotyl glucoside, 1), 2-hydroxy-5-methylbenzoyl-β-l-glucopyranosyl (2 → 1)-β-l-glucopyranosyl (2 → 1)-β-l-glucopyranoside (p-cresotyl triglucoside, 2), and 2-hydroxybenzoyl-β-l-glucopyranosyl (2 → 1)-β-l-glucopyranosyl (2 → 1)-β-l-glucopyranosyl (2 → 1)-β-l-glucopyranoside (salicylic acid tetraglucoside, 5), along with sucrose and triglucoside. The structures of these phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.     

大孔树脂吸附法精制牡丹皮总苷的研究

目的：筛选大孔树脂吸附法精制丹皮总苷的最佳条件.方法：采用动态吸附-解吸方法,用HPLC法测定芍药苷的含量对工艺进行评价.结果：上柱量按1 mL树脂吸附3.2 mg芍药苷计,以6倍柱体积水洗脱;收集40%乙醇洗脱液,洗脱剂用量约为6倍柱体积.丹皮总苷得率为3.7%,含芍药苷为17%.结论：该法简单可行,分离效果好,能满足大生产的要求.     

Bioactive megastigmane glucosides and monoterpenes from Lyonia ovalifolia

Three new megastigmane glucosides (1–3) and two new monoterpenes (4–5), together with 14 related known compounds (6–19) were isolated from the twigs and leaves of Lyonia ovalifolia. The structures of the new compounds were determined by extensive MS, NMR, CD experiments and chemical methods. Compounds 2, 6, and 18 displayed potent antiviral activity against Coxsackie B3, with IC₅₀ values between 6.4 and 14.6 µM. Additionally, compounds 6, 10, and 11 exhibited noteworthy anti-inflammatory activities, with inhibition rates ranging from 54.55% to 83.33% under the concentration of 10^?5 M.     

高效液相色谱法同时测定香青兰提取物中4种黄酮成分的含量

目的:建立RP-HPLC同时测定香青兰提取物中4种黄酮成分含量的方法。方法:Sunfire-C₁₈色谱柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.2%磷酸(B)水溶液为流动相,线性梯度洗脱(0~5 min,16% A;5~45 min,16%→28% A;45~65 min,28% →80% A);流速1.05 mL·min^-1;柱温35℃;检测波长330 nm。结果:香叶木素-7-O-β-D-葡萄糖醛酸苷、田蓟苷、刺槐素-7-O-β-D-葡萄糖醛酸苷和刺槐素质量浓度分别在1.20~24.00μg·mL^-1(r=0.9998),9.03~180.48μg·mL^-1(r=0.9999),1.00~20.00μg·mL^-1(r=0.9999)和0.83~16.64μg·mL^-1(r=0.9999)范围内呈良好的线性关系;平均回收率分别为99.62%,100.11%,98.86%,102.51%,RSD分别为1.10%,1.50%,0.88%,1.72%。结论:该方法简单、准确可靠,可为香青兰提取物中黄酮成分的含量测定提供科学依据。     

Iridoid glucosides from Wendlandia ligustroides (Boiss. &Hohen.) Blakelock

Eight iridoid glucosides were reported from the aerial parts of Wendlandia ligustroides. 10-deoxygeniposidic acid (1), 7-deoxygardoside (2), geniposidic acid (3), 7-deoxy-8-epi-loganic acid (4), deacetyl-daphylloside (5), scandoside methyl ester (6), 6-O-methyl-deacetyl-daphylloside (7), 6-O-methyl-scandoside methyl ester (8). Compounds 3 – 8 were isolated as a pure form while 1 and 2 as a mixture. The structures of the compounds 1 – 8 were established by spectroscopic methods including 1D-NMR (¹H NMR, ¹³C NMR, DEPT-135), 2D-NMR (COSY, NOESY, HSQC, HMBC) and HRMS.