人疱疹病毒8型ORF26基因亚型与鳞状细胞癌的相关性研究

目的 探讨新疆鳞状细胞癌（简称鳞癌）患者感染人疱疹病毒8型（HHV-8） ORF26基因亚型。方法 巢式PCR扩增41例皮肤鳞癌、46例食管鳞癌石蜡包埋组织标本中HHV-8 ORF26基因,对PCR阳性产物进行双向测序,使用Dnastar软件、Clustal W软件和Phylip软件包对测序结果进行系统发生学分析,从而确定HHV-8 ORF26基因亚型。运用SPSS17.0进行统计学分析。结果 41例皮肤鳞癌组织标本9例HHV-8感染为阳性,阳性率为21.95%;46例食管鳞癌10例为阳性,阳性率为21.74%。新疆皮肤鳞癌患者感染HHV-8 ORF26亚型7例为A亚型,2例为C亚型;食管鳞癌感染HHV-8 ORF26亚型7例为A亚型,3例为C亚型。结论 新疆鳞癌患者感染HHV-8 ORF26亚型属于A亚型和C亚型,A亚型多于C亚型,亚型分布与鳞癌患者的发病部位无关。     

人类疱疹病毒8型ORF26基因亚型与Kaposi肉瘤的相关性研究

目的 明确Kaposi肉瘤患者感染的人类疱疹病毒8型（HHV-8）ORF26基因亚型分类，初步探讨其与Kaposi肉瘤不同临床分型及侵袭性的相关性。方法 对32例Kaposi肉瘤石蜡包埋组织进行HHV-8 DNA抽提、扩增、双向测序，使用DNAStar软件、Clustal W软件和PHYLIP软件包对测序结果进行系统发生学分析，从而确定HHV-8 ORF26基因亚型，最后运用Fisher确切概率法对结果进行统计学分析。结果 32例Kaposi肉瘤中有30例HHV-8阳性，阳性率为93.75%，其中6例艾滋病相关型患者HHV-8均阳性。30例HHV-8阳性患者中，17例为HHV-8 ORF26 A亚型，13例为C亚型。不同亚型间Kaposi肉瘤患者有无黏膜损害及临床分型的分布差异均无统计学意义（P > 0.05）。结论 Kaposi肉瘤患者感染HHV-8 ORF26亚型属于A亚型和C亚型，不同亚型与黏膜损害及临床分型无关。     

新疆Kaposi肉瘤患者感染的人类疱疹病毒8型ORF75基因多态性研究

目的通过对人类疱疹病毒8型(HHV-8)ORF75基因多态性研究,初步探讨其与新疆Kaposi肉瘤的相关性。方法对25例新疆Kaposi肉瘤患者石蜡包埋组织进行HHV-8 DNA抽提、扩增及双向测序,使用CLUSTALW软件和PHYLIP软件包对ORF75基因进行核苷酸多态性分析。结果①25例新疆Kaposi肉瘤患者有21例HHV-8感染阳性,阳性率为84%,其中7例艾滋病相关型Kaposi肉瘤(AIDS-KS)患者HHV-8感染均为阳性。②新疆Kaposi肉瘤患者HHV-8 ORF75基因核苷酸具有多态性,存在462、618、647三个主要突变位点。结论人类疱疹病毒8型ORF75基因多态性与新疆Kaposi肉瘤具有相关性。     

人疱疹病毒8型ORF75基因亚型与Kaposi肉瘤的相关性研究

目的 探讨人类疱疹病毒8型(HHV-8)ORF75基因亚型,与Kaposi肉瘤不同临床分型及侵袭性的相关性.方法 对25例新疆Kaposi肉瘤石蜡包埋组织进行HHV-8 DNA抽提、扩增及双向测序,使用Clustal W软件和PHYLIP软件包对测序结果进行发生学分析,从而确定HHV-8 ORF75基因哑型.结果 25例Kaposi肉瘤中,21例HHV-8阳性,阳性率为84%,其中7例AIDS相关型Kaposi肉瘤患者HHV-8均阳性.21例HHV-8阳性患者中,18例为HHV-8 ORF75 A亚型,3例为C亚型;不同亚型间Kaposi肉瘤患者有无黏膜损害及临床分型的分布差异均无统计学意义(P＞0.05).结论 新疆Kaposi肉瘤患者感染HHV-8 ORF75亚型属于A亚型和C亚型,HHV-8 ORF75不同亚型可能与新疆Kaposi肉瘤黏膜损害及临床分型无关.     

人类疱疹病毒8型ORF75亚型的研究进展

人类疱疹病毒8型是新发现的一种人类致瘤病毒,根据不同的开放阅读框架(open reading frames,ORFs)可以把HHV-8分为不同的亚型,近年来对ORF75亚型的大量研究表明其与HHV-8相关疾病的关系密切.     

人疱疹病毒8型-Kaposi肉瘤相关疱疹病毒

从Kaposi肉瘤中检出一种新的疱疹病毒,命名为Kaposi肉瘤相关疱疹病毒,又称人疱疹病毒8型。它可能参与了Kaposi肉瘤的发病机制。并与某些淋巴增生性疾病(body-cavity-based淋巴瘤和多中心Castleman病)有关。故认为它是一个新的DNA肿瘤病毒。     

人疱疹病毒8型—Kaposi肉瘤相关疱疹病毒

从Ｋａｐｏｓｉ肉瘤中检出一种新的疱疹病毒，命名为Ｋａｐｏｓｉ肉瘤相关疱疹冱，又称人疱疹病毒８型，它可能参与Ｋａｐｏｓｉ肉瘤的发病机制，并与某些淋巴增生性疾病（ｂｏｄｙ－ｃａｖｉｔｙ－ｂａｓｅｄ淋巴瘤和多中心Ｃａｓｔｌｅｍａｎ病）有关，故认为它是一个新的ＤＮＡ肿瘤病毒。     

人疱疹病毒8型分子流行病学研究进展

人疱疹病毒8型（HHV-8）的发现在HHV-8及相关疾病的分子生物学的研究方面开辟了一个新领域，然而，HHV-8的流行病学、实验室诊断及致病机制方面都存在着争议，本文就HHV-8的分子流行病学研究进展作一综述。     

人疱疹病毒8型-Kaposi肉瘤相关疱疹病毒及其相关疾病的研究进展

Kapsoi肉瘤相关疱疹病毒,又称人疱疹病毒8型,是近来发现的一种新的肿瘤病毒,它参与了Kaposi肉瘤的发病机制,并与某些血液疾病,如原发性渗出性淋巴瘤及多中心Castleman病等有关.此病毒的一系列病毒基因能干扰细胞的生长和分化,但确切的致瘤因素仍在研究中.     

人疱疹病毒8型—Kaposi肉瘤相关疱疹病毒及其相关疾病的研究进展

Kaposi肉瘤相关疱疹病毒,又称人疱疹病毒8型,最近来发现的一种新的肿瘤病毒,它参与了Kaposi肉瘤的发病机制,并与某些血液疾病,如原发性渗出性淋巴瘤及多中心Castleman病等有关。此病毒的一系列病毒基因能干扰细胞的生长和分化,但确切的致瘤因素仍在研究中。     

Human herpesvirus 8 (HHV-8) in familial Kaposi's sarcoma

Human herpesvirus 8 (HHV-8) has been detected in various epidemiological forms of Kaposi's sarcoma (KS). Since familial KS cases are exceedingly rare and the occurrence of familial KS in siblings has thought to depend rather on genetic factors than on a viral factor, familial KS has not been investigated for the presence of HHV-8. To investigate whether HHV-8 is present also in this rare form of KS, we examined tumor biopsies of 2 siblings with familial KS for the presence of HHV-8 specific DNA sequences by a nested PCR protocol. HHV-8 DNA sequences could be detected in KS specimens of both patients. Sequence analysis revealed an identical DNA sequence of HHV-8 in KS tissue of both siblings, but the sequence in our cases differs in one base pair at position 67 from the previously published HHV-8 KS330Bam fragment. The findings indicate that besides the yet poorly defined genetical factors involved in the pathogenesis of KS, HHV-8 may act as a cofactor also in familial KS. In addition, our data demonstrate that HHV-8 is found in all epidemiological forms of KS, including the rarely occurring familial KS. Familial KS may act as a further model to study the interaction of an oncogenic virus with genetic host factors in the context of a neoplastic disorder.     

Kaposi肉瘤组织内人类疱疹病毒8型的K15基因型研究

目的 了解Kaposi肉瘤组织中人类疱疹病毒8型(HHV-8)K15等位基因型分布情况,并初步探讨Kaposi肉瘤不同临床分型及临床表现与不同HHV-8 K15等位基因型的相关性.方法 采用酚-氯仿-异戊醇法对收集的27例Kaposi肉瘤石蜡包埋组织标本进行病毒DNA抽提,并使用巢式PCR扩增K15基因片段,然后测序并确定其等位基因型.结果 27例Kaposi肉瘤中有22例HHV-8感染为阳性,阳性率为81.48%,其中4例艾滋病-Kaposi肉瘤患者HHV-8感染均为阳性,感染率100%;在分析的22例HHV-8病毒株中,20例为P型,2例为M型;4例艾滋病-Kaposi肉瘤患者感染的均为P型HHV-8,2例M型感染者均为经典型Kaposi肉瘤患者.结论 Kaposi肉瘤组织内HHV-8的K15等位基因型主要是P型,也存在部分M型HHV-8感染者.4例艾滋病-Kaposi肉瘤患者感染的HHV-8均为P型.     

Kaposi肉瘤组织内人疱疹病毒8型的K14.1基因型研究

目的:确定人疱疹病毒8 型K14.1基因等位基因型在Kaposi肉瘤中的分布,及与Kaposi肉瘤临床分型的关系.方法:使用酚-氯仿-异戊醇法对32例Kaposi肉瘤石蜡包埋组织进行病毒DNA提取,使用PCR法扩增K14.1基因片段,然后确定其等位基因型.结果:32 例中有27 例人疱疹病毒8型感染为阳性,阳性率为84.38%,其中5例艾滋病相关型KS患者HHV-8感染均为阳性,感染率100% ;在分析的27 例HHV-8病毒株中,24例为P等位基因型(包括5例艾滋病相关型KS患者皮损),3例为M等位基因型(均来自经典型KS).结论:本研究中,Kaposi肉瘤感染的人疱疹病毒8型的K14.1等位基因型以P等位基因型为主.     

Kaposi's sarcoma and other manifestations of human herpesvirus 8

Kaposi's sarcoma (KS) was described by Moritz Kaposi in 1872 and was known for an entire century as a rare disorder of older men usually of Eastern European, Mediterranean, and/or Jewish origin. In the early 1980s, the prevalence of KS began to increase dramatically and soon became the most common malignancy in patients with AIDS, especially those who were male homosexuals. In 1994, a new human herpesvirus (HHV) was found to be present in almost 100% of KS lesions. This virus was found to be a gammaherpesvirus, closely related to Epstein-Barr virus, and was designated HHV-8. Subsequently, HHV-8 DNA was found in almost all specimens of classic KS, endemic KS, and iatrogenic KS, as well as epidemic KS (ie, AIDS KS). It is now believed that HHV-8 is necessary, but not sufficient, to cause KS and that other factors such as immunosuppression play a major role. The use of highly active antiretroviral therapy (HAART) since 1996 has markedly reduced the prevalence of AIDS KS in western countries, but because 99% of the 40 million patients with AIDS in the world cannot afford HAART, KS is still a very common problem. Primary effusion lymphoma and multicentric Castleman's disease are also thought to be due to HHV-8. Although HHV-8 DNA has been described in a number of other cutaneous disorders, there is little evidence that HHV-8 is of etiologic significance in these diseases. The mechanism by which HHV-8 causes KS, primary effusion lymphoma, and multicentric Castleman's disease is not well understood but is thought to involve a number of molecular events, the study of which should further our understanding of viral oncology. (J Am Acad Dermatol 2002;47:641-55.) Learning objective: At the completion of this learning activity, participants should be familiar with Kaposi's sarcoma and other manifestations of human herpesvirus 8.     

Further confirmation of the association of human herpesvirus 8 with Kaposi's sarcoma

Recently, a new herpesvirus-like DNA sequence named Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV8) has been isolated from almost all cases of Kaposi's sarcoma (KS). It has not been found in most benign and malignant cutaneous hemangioproliferative disorders other than KS. To further verify the specificity of the association of this new viral DNA with KS, we examined in total 42 cases of vascular neoplasms of endothelial derivation using nested polymerase chain reaction (PCR) for the presence of a 233-bp segment of this KSHV/HHV8 on paraffin-embedded specimens. In our investigation, we added an additional step to conventional PCR protocol that uses UV light to pretreat all the PCR regeants except Taq DNA polymerase and the target DNA to eliminate the false positives caused by trace contamination. All 15 cases of typical KS, both AIDS and non-AIDS related, as well as 4 cases of atypical vascular tumors suspicious of KS, were positive for this KSHV/HHV8 DNA sequence. The remaining 23 cases of hemangioproliferative disorders other than KS, including angiosarcoma, capillary hemangioma, angiolymphoid hyperplasia with eosinophilia, epithelioid hemangioma, histiocytoid hemangioma, hemangioendothelioma, and microvenous hemangioma, were negative for HHV8. These results confirm the previous observation that KSHV/HHV8 is specific for KS within hemangioproliferative cutaneous disorders, and PCR for detection of KSHV/HHV8 might be used as an additional diagnostic tool in distinguishing KS.     

Kaposi肉瘤组织内人类疱疹病毒8型K1基因型的初步分析

A5和C7亚型,与非洲和欧洲来源毒株的同源性很高.