胃癌组织中PCNA与微血管密度相关性的研究

目的 :研究胃癌组织中增殖细胞核抗原 (PCNA)与微血管密度 (MVD)的表达情况及其相关性。方法 :选取 6 0份胃癌组织作为实验组 ,同时取 2 0份远癌旁胃黏膜作为对照组 ,用免疫组化SP法检测其PCNA和MVD的表达。结果 :( 1) 6 0份胃癌组织中的PCNA指数平均值为 ( 4 2 0 0± 17 41) % ,MVD均值为 10 0 7± 5 2 3,均显著高于对照组。 ( 2 )PCNA与MVD比较 ,两者呈正相关 (r =0 5 98,P <0 0 0 1)。结论 :胃癌组织中存在有PCNA与MVD的高表达 ,且PCNA与MVD具有相关性 ,反映胃癌细胞增殖活跃 ,正反馈促进肿瘤MVD的增高 ,而使胃癌不断增生和扩张。     

肝癌中Survivin表达与PCNA和AFP的关系及意义

目的 :探讨Survivin在原发性肝细胞癌 (HCC)组织中的表达及与PCNA和AFP的关系。方法 :采用Westernblot和RT -PCR检测 4 3例肝癌组织、2 0例癌旁组织和 11例正常肝组织中Survivin蛋白和mRNA的表达情况。免疫组化 (SP法 )检测Sur vivin、PCNA和AFP蛋白的表达情况。结果 :4 3例HCC标本中分别有 30例表达Survivin蛋白 (6 9.8% ) ,除 1例癌旁组织外 ,其它癌旁组织和正常肝组织中未见Survivin蛋白表达。Survivin表达与肝癌的病理分级、临床分期和AFP的表达等无明显相关。Survivin表达与肝癌细胞增殖活性 (PCNA -LI)显著相关 (P <0 .0 5 )。结论 :Survivin在肝癌组织中高表达 ,提示Survivin在肝癌的发生、发展过程中起重要作用 ;Survivin的表达与肝癌细胞增殖活性 (PCNA -LI)显著相关 ,提示Survivin可能促进肝癌细胞的恶性增殖     

PCNA、VEGF-C在口腔颌面部的淋巴管瘤组织中表达

目的：研究PCNA、VEGF-C在口腔颌面部的淋巴管瘤组织中表达的相互关系。方法应用免疫组化S-P法检测48例口腔颌面部的淋巴管瘤标本，20例正常淋巴结标本PCNA、VEGF-C的表达情况。结果在口腔颌面部的淋巴管瘤组织中PCNA表达阳性率为18．75％（9／48）；VEGF-C阳性率为25％（12／48）。经统计学分析，在淋巴管瘤标本中PCNA、VEGF-C阳性率等无显著性。结论在口腔颌面部的淋巴管瘤组织中PCNA、VEGF-C表达存在着一定的相关性但同一患者口腔颌面部的淋巴管瘤病理组织中PCNA、VEGF-C阳性表达经统计学分析两者呈正相关。PCNA、VEGF-C与口腔颌面部的淋巴管瘤增殖可能相关。     

胃癌中胰岛素样生长因子结合蛋白-2,胰岛素样生长因子-1和增殖细胞核抗原的表达和临床意义

目的:研究IGFBP-2、IGF-1和PCNA在胃癌组织中的表达,探讨其与胃癌临床病理学特征的关系及意义。方法:用免疫组织化学SP法检测60例胃癌患者肿瘤组织和40例正常胃黏膜组织中IGFBP-2、IGF-1和PCNA的表达水平。结果:(1)IGFBP-2、IGF-1和PCNA在胃癌组织中的表达率显著高于在正常胃黏膜组织中的表达(P<0.01)。(2)IGFBP-2、IGF-1和PCNA在有淋巴结转移胃癌中的表达显著高于无淋巴结转移胃癌(P<0.05)。(3)IGFBP-2和IGF-1在胃癌组织中的表达具有明显正相关(P<0.01)。(4)PCNA的表达率在IGFBP-2和IGF-1阳性胃癌中的表达显著高于C-erbB-2阴性的胃癌(P<0.01)。结论:胃癌组织中IGFBP-2水平的升高可能增强IGF-1的表达,两者通过促进肿瘤细胞的增殖与胃癌的发生发展和浸润转移有关。     

食管鳞状细胞癌组织中细胞凋亡与增殖的研究

目的 :探讨食管鳞状细胞癌组织中细胞凋亡、增殖细胞核抗原 (PCNA )之间的关系及与食管癌发生、发展及预后的关系。方法 :47例原发性食管鳞癌手术切除标本为一组 ,2 0例正常食管黏膜组织为对照组 ,采用免疫组织化学方法及TUNEL法检测其PCNA的表达及细胞凋亡。结果 :癌组织中平均增殖指数 (PI)、平均凋亡指数(AI)及PI/AI比值均较正常组高 (P <0 0 5 )。PI随肿瘤病理分级及临床分期的增加以及淋巴结转移和浸润深度的增加而增高 (P <0 0 5 ) ;AI则随肿瘤病理分级的升高而降低 (P <0 0 5 ) ;PI/AI比值随肿瘤病理分级的升高及淋巴结的转移而增加 (P <0 0 5 )。食管鳞癌组织中PCNA高表达组 (PI >5 0 % )的术后 5年生存率低于PCNA低表达组(PI≤ 5 0 % ) (P <0 0 5 )。结论 :PCNA的异常表达状态能较好地反映食管鳞癌的浸润、转移能力 ,对预后判定有一定价值。增殖凋亡失衡贯穿于食管鳞癌发生、发展的整个过程 ,PI/AI比值有可能作为一个监测肿瘤发生的标志     

脑膜瘤血管内皮生长因子与肿瘤增殖活性及瘤周脑水肿关系的研究

目的:研究血管内皮生长因子(VEGF)在脑膜瘤中的表达及其与脑膜瘤增殖活性、微血管密度及瘤周脑水肿(PTBE)的关系。方法:应用免疫组化方法检测36例脑膜瘤组织中VEGF、增殖细胞核抗原(PCNA)、第Ⅷ因子(F8)的表达,测定其阳性细胞数;PTBE 通过术前颅脑 MHI/CT来评估。结果:脑膜瘤 VEGF表达率为 72％(26/36)5例可疑表达 ; PCNA、F8全部表达。VEGF 表达与PTBE 和微血管密度有显著正相关关系,其r分别为 0.878和0.631 ;VECF 表达与 PCNA表达没有相关性,其r为0.087。PCNA 标记指数<25％占97％(35/36)。结论:VEGF 存在于脑膜瘤组织中,在PTBE 和微血管形成方面起着重要的作用。脑膜瘤属于低增殖活性的良性肿瘤。     

缺氧诱导因子-1α的表达及其与大肠癌细胞凋亡及增殖的关系

目的:研究缺氧诱导因子-1α(HIF-1α)在大肠癌中的表达及其与细胞凋亡、增殖的关系.方法:运用免疫组化的方法检测HIF-1α、Bcl-2、增殖细胞核抗原(PCNA)在13例正常大肠组织及50例大肠癌组织中的表达.结果:正常大肠组织HIF-1α均为阴性表达,癌组织中HIF-1α阳性表达率为64.0%.HIF-1α表达与肿瘤浸润、淋巴结转移及Dukes分期相关.Bcl-2在两组中阳性表达率为15.4%、76.0%,癌组织Bcl-2阳性表达率显著高于正常组织.Bcl-2表达与肿瘤大小、分化、Dukes分期无关.癌组织PCNA高增殖活性表达显著高于正常组织.大肠癌增殖程度与肿瘤浸润及Dukes分期相关.大肠癌HIF-1α表达与Bcl-2及PCNA增殖程度呈正相关.结论:HIF-1α抑制大肠癌细胞凋亡,促进其增殖,与肿瘤浸润转移密切相关,在大肠癌发生发展中发挥重要作用.     

增殖细胞核抗原在子宫颈鳞癌组织中的表达

【目的】了解正常子宫颈鳞状上皮细胞及癌细胞的增殖活性 ,判断增殖细胞核抗原 (PCNA)与宫颈鳞癌生物学行为及预后的关系。【方法】应用免疫组化SP法 ,检测PCNA在 5 2例宫颈鳞癌 ,5例宫颈非典型性增生及8例正常宫颈组织中PCNA的表达。【结果】宫颈鳞癌PCNA强阳性表达率为 44 .2 9% ,明显高于正常宫颈的0 % ,差异有显著性 (P <0 .0 5 )。其免疫反应产物定位于细胞核。宫颈鳞癌中阳性细胞分布不均 ,癌巢周边及前缘浸润细胞阳性率高 ,癌巢中央表达极低 ;分化差的肿瘤 ,癌旁增生严重 ,周围淋巴细胞及淋巴滤泡生发中心均有阳性表达。非典型增生的宫颈组织中 ,阳性细胞分布有规律 ,大多在基底层 ,部分达棘细胞层。正常宫颈组织着色仅限于基底细胞。PCNA在宫颈鳞癌组织中的强阳性表达随病理分级的增高而明显提高 ,差异具有显著性 (P <0 .0 5 )。宫颈鳞癌临床分期越晚 ,PCNA强阳性表达率越高 ,但未显示统计学差异 (P >0 .0 5 )。【结论】PCNA是反映子宫颈鳞癌生物学行为的重要指标之一。     

基质金属蛋白酶-9和增殖细胞核抗原在膀胱移行细胞癌中的表达及相关性

目的:探讨基质金属蛋白酶-9(MMP-9)和增殖细胞核抗原(PCNA)在膀胱移行细胞癌(BTCC)中的表达及临床意义.方法:采用免疫组织化学SP法,检测58例BTCC和10例正常膀胱组织中MMP-9和PCNA的表达.结果:在10例正常膀胱组织中MMP-9和PCNA呈阴性或弱阳性表达.在58例BTCC中,两者阳性表达率分别为65.5%和69.0%,明显高于正常膀胱组织(P<0.01).MMP-9、PCNA在低级别BTCC中的阳性表达率分别为35.3%和29.4%,明显低于高级别BTCC的78.0%和85.4%(P<0.01);MMP-9、PCNA在浅表性BTCC中的阳性表达率分别为36.4%和40.9%,明显低于浸润性BTCC的83.3%和86.1%(P<0.01).MMP-9、PCNA在BTCC中的表达呈正相关(r=0.451,P<0.01).结论:MMP-9、PCNA在BTCC的发生、分化、浸润和转移过程中发挥重要作用,两者联合检测有利于评估BTCC的恶性程度和预后.     

PCNA在子宫颈上皮内瘤变及宫颈癌中的表达及其意义

增殖细胞核抗原(PCNA)是一种反映细胞增殖活性的标志物。为了探讨PCNA在子宫颈上皮内瘤变(CIN)及子宫颈鳞癌中的表达及其临床意义,作者等采用免疫组化方法对患有慢性宫颈炎的宫颈组织,子宫颈上皮内瘤以及子宫颈鳞癌组织中PCNA表达状态进行了检测。     

Assessment of salivary and serum antioxidant vitamins and lipid peroxidation in patients with recurrent aphthous ulceration

Recurrent aphthous ulceration (RAU) is a common oral mucosal disorder characterized by recurrent, painful oral aphthae. Although the exact cause of RAU is not known, local trauma, microorganisms, nutritional deficiencies, hormonal changes, genetics, and immunological factors have been suggested to contribute to its pathogenesis. The aim of this study was to assess the level of lipid peroxidation and status of antioxidant vitamins in patients with RAU. Thirty patients with RAU and 20 healthy controls were recruited. Vitamins A, E, and C and malondialdehyde (MDA) levels were measured in both serum and saliva of patients with RAU and control subjects by high performance liquid chromatography. Levels of vitamins A, E and C in both fluids were significantly lower (p < 0.05 for vitamins A and E, and p < 0.005 for vitamin C, respectively) in patients with RAU than in healthy control subjects. Conversely, the levels of MDA in serum and saliva were significantly higher (p < 0.005) in patients with RAU than in the control group. Furthermore, strong and highly significant correlation was found between serum and salivary levels of vitamins A, E and C, and MDA in patients with RAU (r > or = 0.90, p < 0.0001). The present study demonstrates that the serum and saliva levels of selected antioxidant vitamins are lower, while the degree of lipid peroxidation, as judged by the MDA levels, is higher in patients with RAU than in the control subjects. This is the first to measure specific antioxidant levels in both saliva and blood in the same patients, and indicates that the non-enzymatic anti-oxidant ability is impaired in patients with RAU.     

TNF-α在复发性阿弗他溃疡患者唾液中的表达及临床意义

目的研究在复发性阿弗他溃疡(RAU)患者唾液中肿瘤坏死因子(TNF-α)的表达及临床意义。方法用双抗体夹心ELISA法对RAU患者38例(男21例,女17例,年龄15～56岁)唾液中TNF-α的含量进行检测,并以38例(男19例,女19例,年龄18～50岁)健康人做正常对照,对结果进行定量分析。结果 38例RAU患者唾液中TNF-α的平均含量(82.86±21.63)pg/mL比健康人(55.34±17.50)pg/mL明显升高(t=3.877,P<0.001)。结论 TNF-α的含量在RAU患者唾液中明显高于健康对照组,说明TNF-α参与了RAU的病理过程。     

复发性口疮患者雷公藤多甙治疗前后血清一氧化氮、一氧化氮合酶水平的变化

目的观察复发性口疮(RAU)患者用雷公藤多甙治疗前后血清一氧化氮(NO)、一氧化氮合酶(NOS)水平的变化,为治疗及预后判断提供依据。方法应用硝酸还原酶法对132例经临床确诊的RAU患者治疗前后和60名正常对照者血清NO、NOS水平分别进行检测。结果在治疗前RAU患者血清NO、NOS水平明显高于正常人,经雷公藤多甙治疗症状缓解或消失后,血清NO、NOS下降至正常人水平,RAU患者治疗前后血清中NO、NOS水平差异有显著性(P<0.01)。结论NO、NOS的动态检测对其疗效判断有一定的价值。     

早期镇痛疗法治疗复发性口腔溃疡的临床研究

目的:探讨早期镇痛疗法对复发性口腔溃疡(RAU)的疗效及临床意义.方法:选择复发性口腔溃疡患者60例,随机分为两组,每组30例.治疗组口服万络,50mg/天;对照组口服替硝唑1.0g/天,观察镇痛疗法对溃疡愈合的影响.结果:治疗、对照组用药2天的痊愈率:治疗组为13.33%,对照组为0%,有显著性差异(P＜0.05);治疗、对照组2天总有效率分别为80%和10%,有显著性差异(P＜0.05);治疗4天的痊愈率,镇痛治疗组为73.33%,明显高于对照组33.33%,有显著性差异(P＜0.05);治疗、对照组4天总有效率分别为93.33%和80%,差异不显著(P＞0.05).结论:早期镇痛疗法治疗RAU具有缓解持续顽固性疼痛、快速治愈口腔溃疡、改善进食状况的作用.     

HLA-A,B (Class I) and HLA-DR,DQ (Class II) Antigens in Turkish Patients with Recurrent Aphthous Ulceration and Beh?et's Disease

Objective

The aims of the present study were to typify the human leukocyte antigen system (HLA)-A, B (class I) and HLA-DR, DQ (class II) antigens and to assess the frequency of the presence of these antigens in the Turkish population with recurrent aphthous ulceration (RAU) and Behçet''s disease (BD) compared to healthy subjects.

Subjects and Methods

Thirty patients with RAU, 30 with BD, and 15 healthy subjects were included in the study. HLA typing was performed by serology with commercial kits for HLA class I and II (One Lambda, Canoga Park, Calif., USA).

Results

The HLA-A23 frequency was 26.7% in the RAU patients, which was significantly higher than the 3.3% frequency in the patients with BD (p < 0.05). The HLA-A24 frequency was 33.3% in the RAU patient group, which was significantly higher (p < 0.05) than the frequency in the healthy subjects (6.7%). Significantly higher frequencies (46.7%) of HLA-A30 were found in the healthy subjects compared to the BD (13.3%) and RAU (3.3%) patients (p < 0.05 and p < 0.01, respectively). A higher frequency of HLA-B13 was observed in the RAU (23.3%) patients compared to the BD (0%) patients (p < 0.01). A decrease was observed in HLA-DR10 and HLA-DR17 in the RAU patients (p < 0.05), while a higher frequency of HLA-DR10 was observed in the BD patients compared to the RAU patients (p < 0.01).

Conclusions

These results showed that RAU and BD were not in the same spectrum and the involvement of other genetic and/or environmental factors might be responsible for the development of these diseases and/or disease progression.Key Words: Recurrent aphthous ulceration, Behçet''s disease, HLA class I, HLA class II, Antigen     

Relationships of interleukin-17 polymorphisms with recurrent aphthous ulcer risk in a Han Chinese population

ObjectiveInterleukin (IL)-17 is a multifunctional cytokine with important roles in inflammatory and autoimmune diseases. This case–control study explored the relationships of IL-17A rs2275913 and IL-17F rs763780 single-nucleotide polymorphisms (SNPs) with recurrent aphthous ulcer (RAU) morbidity and severity.MethodsIL-17A rs2275913 and IL-17F rs763780 SNPs were measured in 125 patients with RAU and 116 healthy control participants. The genotype distributions, disease risks, and relationships with RAU severity were analyzed.ResultsRAU risk was associated with rs2275913 after adjustment for age, body mass index, sex, smoking status, and drinking status (AA vs. GG: odds ratio [OR], 2.759; 95% confidence interval [CI], 1.381–5.512; A allele vs. G allele: OR, 1.783; 95% CI, 1.242–2.560). TC and CC genotypes in rs763780, and the corresponding C allele, demonstrated greater prevalence among patients with RAU, compared with the TT genotype (TC vs. TT, OR: 1.895; 95% CI: 1.088–3.301; CC vs. TT, OR: 4.080, 95% CI: 1.079–15.425; C allele vs. T allele, OR: 1.969, 95% CI: 1.257–3.083). Serum IL-17 concentrations were also higher in patients with RAU than in control participants. These concentrations were associated with IL-17 polymorphisms.ConclusionsIL-17 polymorphisms might be associated with greater risk of RAU pathogenesis.     

Common lesions of the oral mucosa. A guide to diseases of the lips, cheeks, tongue, and gingivae.

Canker sores and cold sores are common, relatively banal diseases of the oral mucosa and lips, occurring most often in young persons. Some otherwise healthy patients may have a more severe variant, such as major aphthous ulcers, recurring lesions of aphthous stomatitis, or acute herpetic gingivostomatitis. Other patients may present with severe recurrent herpes simplex labialis or chronic oral candidiasis, and in these patients an immunodeficiency state must be considered.     

血清NO及NOS在雷公藤多甙治疗复发性口疮的疗效评价中的应用

目的 通过对运用雷公藤多甙（TWP）治疗复发性口疮（RAU）的临床观察,探讨血清一氧化氮（NO）、一氧化氮合酶（NOS）水平的变化及其在临床疗效和预后判断中的应用价值。方法 用常规治疗方案和TWP联合治疗方案治疗RAU患者108例及135例。观察治疗前后血清NO、NOS水平变化,分别作治疗期疗效和治疗后疗效变化评价。结果 TWP联合治疗组,近期疗效有效率为91．11％,远期疗效有效率为94．07％,常规治疗组近期疗效有效率为70．37％,远期疗效有效率为68．52％。结论 血清NO、NOS水平变化可用于治疗RAU的疗效评价和预后判断。     

中西医结合治疗复发性口疮临床观察

目的:观察局部微波及口服口炎清颗粒治疗复发性口疮(RAU)的临床疗效。方法:将96例RAU患者随机分成三组,第一组口服口炎清颗粒联合微波治疗溃疡面,第二组采用微波治疗溃疡面,第三组口服口炎清颗粒治疗。结果:在平均溃疡期缩短和止痛方面:总有效率第一组为96.67%,第二组为87.50%,第三组为64.52%,第一、二组优于第三组,尤其第一组;在减少RAU复发方面:总有效率第一组为76.67%,第二组为34.38%,第三组为61.29%,第一、三组优于第二组,尤其第一组。结论:口炎清颗粒联合微波治疗复发性口疮能减轻疼痛、促进愈合和减少复发,是安全有效的治疗方法。