男性人乳头瘤病毒检测方法的建立及临床应用

目的:建立一种适合临床男性人乳头瘤病毒(HPV)基因亚型检测的新方法,并通过检测棉拭子采集男性尿道口分泌物来验证该方法的临床检测效果。方法:通过计算机辅助,参考经典通用引物(MY09/11)和加以改进的PGMY09/11设计23种HPV基因亚型的PCR扩增引物,根据GenBank中的HPV的型特异性序列设计及合成探针,制备可同时对18种高危型:HPV-16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83,MM4和5种低危型:6,11,42,43,44进行分型检测的特定纸质膜芯片,并且对112例采用棉拭子采集的男性尿道口分泌物进行检测加以验证,同时,对单一阳性标本进行测序验证,对标准品进行灵敏度检测。结果:112例男性尿道口分泌物中检测出25例HPV阳性标本,其中单一HPV亚型感染13例,多重感染12例,检测出的男性HPV基因亚型有:HPV-6,11,16,18,33,35,43,56和73型九种。灵敏度可达10个拷贝HPVDNA分子。结论:该方法适用于临床进行男性HPV感染的诊断以及为开展相应的流行病、病因学调查提供切实可行的检测手段。     

100例男性尖锐湿疣皮损人乳头瘤病毒基因亚型的分布及意义

目的 了解无锡男性尖锐湿疣皮损HPV基因亚型的分布状况.方法 采用基因分型芯片方法检测23种基因亚型,高危型18种:HPV-16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83,MM4和低危型5种:HPV-6,11,42,43,44.结果 100例临床标本中阳性率为98%,检出率最高依次为11型、6型、16型.25%患者存在多重感染.结论 HPV11、6、16型感染是男性尖锐湿疣发病的主要型别,且HPV感染趋于多重化.     

育龄男性泌尿生殖道解脲支原体和人乳头瘤病毒感染及影响因素分析

目的:了解育龄男性泌尿生殖道解脲支原体(UU)和人乳头瘤病毒(HPV)感染的情况,探讨可能的影响因素,为今后开展生殖道感染的预防提供科学依据。方法:2016年8月至2017年7月,采用分阶段整群抽样的方法,选择上海市松江区的工厂、企事业单位和服务场所的18～50岁的育龄男性,对其进行结构式问卷调查,并采集泌尿生殖道分泌物标本,分别采用培养法和PCR方法进行UU和HPV的检测。结果:本研究共纳入621例育龄男性,泌尿生殖道UU阳性率为44.93%(279/621),HPV感染率为2.90%(18/621),UU和HPV双重感染率为2.42%(15/621)。单因素分析显示吸烟者的UU感染率(50.54%,140/277)高于不吸烟者(40.41%,139/344);高中/技校研究对象HPV感染率(4.84%,12/248)略高于其他文化程度者。二元逐步Logistic回归分析发现:与初中及以下研究对象相比,大专及以上者UU感染的风险较低(OR=0.61,95%CI:0.39～0.96);吸烟者UU阳性的风险高于不吸烟者(OR=1.46,95%CI:1.01～2.01)。结论:上海松江地区育龄男性的UU感染率较高,HPV感染率较低。应加强对文化程度较低(初中及以下)、吸烟育龄男性的UU筛查,以期降低UU的阳性率。     

包皮环切后包皮龟头炎、包皮垢及人乳头瘤病毒感染的年龄分布调查

目的：探讨不同年龄包皮环切后人群包皮中包皮龟头炎、包皮垢及人乳头瘤病毒（ Ｈ Ｐ Ｖ）感染的发生率。　方法：对 ２１９ 例包皮环切后通过问诊和体检，了解包皮龟头炎和包皮垢的情况，采用 Ｐ Ｃ Ｒ 扩增的方法检测包皮中 Ｈ Ｐ Ｖ ６、１１、１６、１８ 及 ３３ 型 Ｄ Ｎ Ａ 保守片段。　结果：包皮龟头炎、包皮垢及 Ｈ Ｐ Ｖ 感染的发生率分别为 ２１．０％（４６／２１９）、４３．３％ （９５／２１９）和４１．１％ （９０／２１９）。　结论：包皮环切后包皮中包皮龟头炎、包皮垢及 Ｈ Ｐ Ｖ 感染的发生率较高，对于包茎或包皮过长者，应尽早行包皮环切术。     

Prevalence of human papillomavirus infection in the urinary tract of men with urethritis

Objectives: To investigate the prevalence of human papillomavirus (HPV) in the genital and urinary tract of men with urethritis. Methods: Cell samples were collected from the penis, urethra and urine of 142 men with urethritis. A HPV test was performed on the samples using the modified GP5+/6+ polymerase chain reaction method, and the HPV genotype was determined using a HPV GenoArray test. Results: Out of 142 urethritis patients, HPV was detected in 48% (68 cases), and high‐risk HPV was found in 32% (46 cases) of patients, on their penis or in the urinary tract (urethra or urine). HPV was detected in 31% in the penis, 20% in the urethra and 24% in the urine, while high‐risk HPV was identified in 23% in the penis, 12% in the urethra and 11% in the urine. Among the HPV‐positive men, 66% had HPV infection in the urinary tract where the most common HPV types were HPV6, HPV16, HPV18 and HPV58. Single HPV‐type infection was more frequently found in the urinary tract (89%) than in the penis (65%) (P < 0.05). Conclusions: Similar to the penis, the urinary tract represents a common HPV infection site in men with urethritis.     

慢性宫颈炎合并人乳头瘤病毒感染的临床研究

目的探讨微波联合保妇康栓治疗宫颈糜烂合并人乳头瘤病毒（HPV）感染的临床疗效及安全性。方法将84例宫颈糜烂合并HPV感染者随机分为观察组和对照组,每组各42例;观察组患者采用微波联合保妇康栓治疗,对照组患者采用复方氯霉素阴道泡腾片联合微波治疗,分别比较两组患者的临床疗效和宫颈脱落细胞检测HPV感染情况。结果两组患者治疗后阴道分泌物清洁度均显著提高,但差异无统计学意义（χ2=0.213,P=0.645）;对Ⅰ度和Ⅱ度宫颈糜烂疗效,两组患者有效率相近,差异无统计学意义（P＞0.05）;Ⅲ度宫颈糜烂患者中,观察组较对照组疗效佳,差异具有统计学意义（χ2=3.909,P=0.048）。比较两组患者的HPV低于检测下限的比率,观察组显著优于对照组,差异具有统计学意义（χ2=4.200,P=0.040）。结论微波联合保妇康栓治疗宫颈糜烂合并HPV感染疗效显著,且不良作用发生率低,值得推广。     

Comparison of urinary and seminal N-acetyl-beta-glucosaminidase isoenzymes

N-acetyl-beta-glucosaminidase (NAG) and its isoenzymes were measured in the urine and seminal plasma of healthy volunteers. Urinary NAG level was 2.62 +/- 1.30 U/L (mean +/- standard deviation), 1.99 +/- 0.77 U/g creatinine and seminal NAG level was 2370 +/- 1007 U/l. Urinary NAG level was 2.59 +/- 1.44 U/l, 1.93 +/- 0.80 U/g creatinine in males and 2.67 +/- 1.15 U/l, 2.08 +/- 0.78 U/g creatinine in females, and there was no significant sex difference. NAG isoenzymes in the urine and seminal plasma were divided into two major peaks, A and B. The A:B ratio was 78.0 +/- 6.5: 21.9 +/- 6.5 in the urine and 24.7 +/- 3.2: 75.2 +/- 3.2 in the seminal plasma, and was significantly different. Urinary NAG isoenzyme was 80.3 +/- 6.7: 19.6 +/- 6.7 in males and 74.1 +/- 4.3: 25.9 +/- 4.3 in females, and there was no significant difference between the sexes. These results indicated that urinary and seminal NAG can be differentiated by measuring the isoenzymes. Furthermore, the comparison of seminal NAG isoenzymes before and after vasectomy indicated that seminal NAG may be affected not only by the sperm but also by the prostatic fluid.     

深圳计划生育门诊中男性人乳头状瘤病毒感染状况

目的了解深圳计划生育门诊中男性HPV感染的流行型别和分布状况。方法采用基因分型芯片方法检测23种基因亚型，高危型18种：HPV-16，18，31，33，35，39，45，51，52，53，56，58，59，66，68，73，83，MM4和低危型5种：HPV-6，11，42，43，44。结果112例临床标本中25例阳性，深圳男性HPV检测阳性率是22．3％，最常见的HPV基因亚型是6、16和73型。就诊的男性以31-40岁的年龄组最多，其次是21-30岁年龄组，但发现年龄与男性HPV的感染无相关性。多重HPV感染中，以双重感染33／73型感染最多，高危型和低危型混合感染最常见。结论本次检测可知深圳计划生育门诊中男性HPV最常见的HPV基因亚型是6、16和73型，同时，发现年龄与男性HPV的感染无相关性。     

Penile human papillomavirus infection in consorts of women with genital human papillomavirus infection. A pilot study

A study determined the incidence of human papillomavirus (HPV) on genitalia of male consorts of women with histologically proven genital HPV lesions to be 50%.     

不同人乳头状瘤病毒检测方法对宫颈高度病变的诊断价值

目的 评价杂交捕获Ⅱ代(hc-Ⅱ)与聚合酶链反应(pcr)技术对人乳头状瘤病毒(human papillomavirus,hpv)dna检测及宫颈高度病变的诊断价值.方法 选取宫颈薄层液基细胞学检查(tct)异常的200例妇女为研究对象,同时用hc-Ⅱ、导流杂交基因芯片技术(hybrimax)、实时荧光定量pcr法(fq-pcr)和流式荧光杂交法检测宫颈细胞hpv dna.以病理组织学检测结果为金标准,比较4种检测方法对宫颈上皮内瘤变(cin)≥Ⅱ的诊断效果.采用spss 13.0软件进行统计学处理.结果 hybrimax、fq-pcr、流式荧光法和hc-Ⅱ对200例hpv dna阳性检出率分别为72.5%(145/200)、71.5%(143/200)、70.0%(140/200)和69.0%(138/200).4种hpv dna检测方法对不同程度宫颈病变的hpv检测阳性率差异无统计学意义(x2=0.252、0134、0.012和0.027,p值均＞0.05),但在诊断cinⅡ及以上病变的敏感度、约登指数和阴性预测值等方面比较差异有统计学意义(x2=7.923、7.819和8.108,p＜0.05).结论 对组织病理学诊断结果为cinⅡ及以上的宫颈病变,hc-Ⅱ的诊断价值优于pcr方法. abstract： objective to evaluate the application of different assays for detection of human papillomavirus(hpv)in diagnosis of high grade cervical lesions.methods two hundred subjects with abnormal thinprep liquid-based cytology test(tct)results were selected for hpv dna detection by hybrid capture 2(hc-Ⅱ) and methods based on pcr including flow-through hybridization and gene chip (hybrimax),real-time fluorescent quantitative pcr(fq-pcr)and flow fluorescent hybridization assay.cytopathological results were used as gold standards to evaluate the test performance of the above assays for diagnosing cervical intraepithelial neoplasia(cin)≥Ⅱ. spss 13.0 software was used for statistical analysis.results hpv dna positive rates of 200 samples by hybrimax,fq-pcr,flow fluorescent hybridization assay and hc-Ⅱ were 72.5%(145/200),71.5%(143/200),70.0%(140/200)and 69.0%(138/200),respectively,and the differences were not statistically si(g)nificant(x2 =0.252,0134,0.012 and 0.027,p ＞ 0.05).the sensitivity,youden index and negative predictive value of the above assays were statistically different(x2 =7.923,7.819 and 8.108,p ＜0.05).conclusion hc-Ⅱ is superior to pcr methods in diagnosis of cin Ⅱ and above.     

Agenesis of seminal vesicles in infertile males: ultrasonic diagnosis.

We present the incidence of seminal vesicle agenesis and its association with deferens ductus agenesis in 141 males presenting with infertility. Transrectal ultrasonography was performed in every patient. Ten presented seminal vesicle agenesis (8 unilateral and 2 bilateral). Three had absence of the vas deferens on physical examination (3 of 7 with unilateral seminal vesicle agenesis and 1 of 2 with bilateral). Computed tomography confirmed the ultrasound findings. We emphasize the importance of the anomalies as a cause of infertility and the association with other genitourinary anomalies. Moreover, we state the necessity of transrectal ultrasound in the diagnosis.