卵叶银莲花全草中的新三萜皂苷

目的　对卵叶银莲花（Anemone begoniifolia Lévl.et Vant）全草的的三萜皂苷成分进行分离和结构鉴定。方法　通过正相和反相硅胶柱色谱分离甲醇提取物中的三萜皂苷;利用各种波谱技术并结合化学降解方法鉴定其化学结构。结果　分离鉴定了1个二元混合物和2个单体,二元混合物中的2个成分分别鉴定为3-O-β-D-吡喃葡糖(1→2)-α-L-吡喃阿拉伯糖基-齐墩果酸-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖苷(1)和3-O-β-D-吡喃葡糖(1→3)-α-L-吡喃阿拉伯糖基-齐墩果酸-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖苷(2),2个单体分别鉴定为23-O-丙二酸单甲酯单酰基-常春藤配基-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖苷(3)和3-O-丙二酸单甲酯单酰基-常春藤配基-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖苷(4)。结论　化合物2,3和4为新三萜皂苷,分别命名为卵叶银莲花苷A,B和C。     

东北刺人参叶中四种新三萜皂苷的分离与结构鉴定

目的研究东北刺人参的三萜皂苷化学成分。方法采用大孔树脂、硅胶、ODS柱色谱分离纯化，经理化常数、光谱学方法鉴定结构。结果分离得到了4个新三萜皂苷，其结构分别鉴定为3-O-β-D-吡喃葡糖基3β，23-二羟基羽扇豆-20(29)-烯-28-酸-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡糖基(1→6)-β-D-吡喃葡糖苷(1);3-O-β-D-吡喃葡糖基常春藤皂苷元-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡糖基(1→6)-β-D-吡喃葡糖苷(2);3-O-β-D-吡喃葡糖基3β-羟基齐墩果-9(11)，12-二烯-28-酸-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡糖基(1→6)-β-D-吡喃葡糖苷(3);3α-羟基齐墩果-12-烯-23，28-二酸-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡糖基(1→6)-β-D-吡喃葡糖苷(4)。结论化合物1～4均为新三萜皂苷，首次从东北刺人参叶中分得。     

南重楼Paris vietnamensis活性物质的分离与鉴定

目的研究南重楼Paris vietnamensis (Takht.)中具有细胞毒活性的甾体皂苷类化学成分。方法采用柱色谱进行分离纯化，通过光谱分析和理化方法鉴定化合物结构，并进行体外细胞毒活性筛选。结果从南重楼中分离得到11个甾体皂苷类化合物，化合物1结构为：3β,5α,6α-三羟基-7(8)-烯-异螺甾烷醇-3-氧-β-D-葡吡喃糖基(1→3)［α-L-鼠李吡喃糖基(1→2)］-β-D-葡吡喃糖苷(1)，命名为南重楼皂苷A。化合物2～11分别为：25(R)-薯蓣皂苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)-β-D-葡吡喃糖苷(2)；25(R)薯蓣皂苷元-3-氧-α-L-鼠李糖吡喃基(1→2)-β-D-葡吡喃糖苷(3)；25(R)薯蓣皂苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)［α-L-鼠李吡喃糖基(1→2)］-β-D-葡吡喃糖苷(4)；25(R)薯蓣皂苷元-3-氧-β-D-葡吡喃糖基(1→3)［α-L-鼠李吡喃糖基-(1→2)］-β-D-葡吡喃糖苷(5)；25(R)薯蓣皂苷元-3-氧-α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→4)［α-L-鼠李吡喃糖基(1→2)］-β-D-葡吡喃糖苷(6)；25(R)偏诺苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)-β-D-葡吡喃糖苷(7)；25(R)偏诺苷元-3-氧-α-L-鼠李吡喃糖基(1→2)-β-D-葡吡喃糖苷(8)；25(R)偏诺苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)［α-L-鼠李吡喃糖基(1→2)］-β-D-葡吡喃糖苷(9)；25(R)偏诺皂苷元-3-氧-β-D-葡吡喃糖基(1→3)［α-L-鼠李吡喃糖基(1→2)］-β-D-葡吡喃糖苷(10)；25(R)偏诺苷元-3-氧-α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→4)［α-L-鼠李吡喃糖基(1→2)］-β-D-葡吡喃糖苷(11)，体外筛选表明部分甾体皂苷类化合物具有细胞毒作用。结论化合物1为一新化合物，化合物2，3，6～11均为首次从南重楼中获得。化合物3，4，6，8具有对肝癌HepG2和胃癌SGC-7901的细胞毒活性。     

北野菊黄酮类成分研究

从北野菊(Dendranthema lavandulifolium)全草中分得四个黄酮类化合物。经光谱(UV,IR,氢谱,碳谱,质谱等)方法鉴定其结构,分别为木樨草素(I),洋芹素(I),金合欢素-7-O-α-L-鼠李糖基(1→6)-β-D-葡萄糖甙(II)和金合欢素-7-O-α-L-鼠李糖基(1→6)-[2-O-乙酰基-β-D-葡萄糖基(1→2)]-β-D-葡萄糖甙(IV)。其中,I和I为首次从该植物中得到,IV为一个新化合物。     

Flavonoids from flowers of Malva crispa L. (Malvaceae)

The following flavonoids were isolated and identified from the flowers of Malva crispa L.: kaempferol 3-O-beta-glucopyranoside, 3-O-(6"-tran-p-coumaroyl)-beta-D-glucopyranoside, 7-O-beta-D-glucopyranoside, 3-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside and 3,7-O-diglucoside as well as quercetin 3-O-beta-D-glucopyranoside, 3-O-alpha-L-rhamnopyranosyl (1-->6) -beta-D-glucopyranoside and apigenin 7-O-beta-D-glucopyranoside. Their structures were established by chemical analysis, UV, 1H and 13C NMR spectrometry.     

中药白头翁地上部分的三萜皂苷成分

为了研究白头翁地上部分的三萜皂苷成分，采用不同色谱技术对白头翁地上部分乙醇提取物的正丁醇部位进行分离纯化，并用波谱和化学方法鉴定化合物的结构。从正丁醇部位中共分离得到7个三萜皂苷类化合物，其结构分别被鉴定为2β-羟基常春藤皂苷元28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖酯苷(1)、3-O-α-L-吡喃阿拉伯糖常春藤皂苷元28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖酯苷(2)、3-O-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖齐墩果酸28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖酯苷(3)、3-O-α-L-吡喃鼠李糖(1→2)［β-D-吡喃葡糖(1→4)］-α-L-吡喃阿拉伯糖常春藤皂苷元28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖酯苷(4)、3-O-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖常春藤皂苷元28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖酯苷(5)、常春藤皂苷元28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡糖(1→6)-β-D-吡喃葡糖酯苷(6)及常春藤皂苷元3-O-α-L-吡喃鼠李糖(1→2)-α-L相似文献   

Pregnane glycosides from Dracaena cochinchinensis

Two new pregnane glycosides, named dracaenoside C and D, were isolated from the fresh stems of Dracaena cochinchinensis. Their structures were established as 3beta,14alpha-dihydroxypregna-5,16(17)-diene-20-one 3-O-alpha-L-rhamnopyranosyl(1 --> 2)[alpha-L-rhamnopyranosyl(1 --> 4)]-beta-D-glucopyranoside (1) and 3beta,14alpha-dihydroxypregna-5,16(17)-diene-20-one 3-O-alpha-L-rhamnopyranosyl(1 --> 2)[beta-D-glucopyranosyl(1 --> 3)]-beta-D-glucopyranoside (2) by means of 2D NMR spectral and chemical methods.     

Two unusual oleanane saponins from Anemone anhuiensis

From the rhizomes of Anemone anhuiensis (Ranunculaceae), two new triterpene saponins, anhuienosides A (1) and B (2), were isolated. These compounds are glycosylated at C-23 and their structures were elucidated as hederagenin 23-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside (1) and 23-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl hederagenin 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (2) on the basis of chemical and spectral evidence.     

Antioxidative flavonoids from the leaves ofMorus alba

Nine flavonoids (1-9) were isolated from the leaves of Morus alba (Moraceae). The structures of compounds were determined to be kaempferol-3-O-beta-D-glucopyranoside (astragalin, 1) kaempferol-3-O-(6"-O-acetyl)-beta-D-glucopyranoside (2), quercetin-3-O-(6"-O-acetyl)-beta-D-glucopyranoside (3), quercetin-3-O-beta-D-glucopyranoside (4), kaempferol-3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (5), quercetin-3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (rutin, 6), quercetin-3-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (7), quercetin-3,7-di-O-beta-D-glucopyranoside (8) and quercetin (9) on the basis of spectroscopic and chemical studies. Compounds 7 and 9 exhibited significant radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radical.     

Chemical constituents from the<Emphasis Type="Italic">Hydrangea chinensis</Emphasis>

Two quinazolone alkaloids, (+)-febrifugine (1) and isofebrifugine (2), along with three coumarin derivatives, 6-hydroxy coumarin (3), skimmin (5), and umbelliferone-7-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranoside (6), were isolated from the roots of Hydrangea chinensis. Compound 6 is a new compound. In addition, umbelliferone (4), linoleic acid (7), two steroidal glycosides (8, 9), three furfural derivatives (10-12), and butyl-beta-D-fructofuranoside (13) were isolated from the leaves of the same plant. The structures of all isolates were elucidated by spectral methods.     

Flavonoids and free phenolic acids from Phytolacca americana L. leaves

In the leaves of Phytolacca americana L. flavonoid compounds: kaempferol 3-O-beta-D-glucopyranoside, kaempferol 3-O-beta-D-xylopyranosyl (1-->2)-beta-D-glucopyranoside, kaempferol 3-O-alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranoside, kaempferol 3-O-diglucoside and quercetin 3-O-glucoside were identified by chemical and spectroscopic methods and using co-chromatography, phenolic acids: p-hydroxybenzoic, vanillic, synapic, p-coumaric ferulic and caffeic were also confirmed.     

New furostanol glycosides from the rhizomes of Dioscorea futschauensis R. Kunth

Two new furostanol glycosides, 26-O-beta-D-glucopyranosyl-3beta,26-dihydroxy-23(S)-methoxyl-25(R)-furosta-5,20(22)-dien-3-O-alpha-L-rhamnopyranosyl(1 --> 2)-[beta-D-glucopyranosyl(1 --> 3)]-beta-D-glucopyranoside (dioscoreside E, 1) and 26-O-beta-D-glucopyranosyl-3beta,26-dihydroxy-25(R)-furosta-5,20(22)-dien-3-O-alpha-Lrhamnopyranosyl(1 --> 2)-[beta-D-glucopyranosyl (1 --> 3)]-beta-D-glucopyranoside (prtotogracillin, 2), together with 11 known furostanol glycosides were isolated from the rhizomes of Dioscorea futshauensis R. Kunth. Their structures were elucidated on the basis of spectroscopic analysis (NMR and FABMS). Their anti-fungal activity against the plant pathogenic fungus Pyricularia oryzae and cytotoxic activity on K562 cancer cell line were evaluated in vitro.     

Flavonoids from Prunus serotina Ehrh

In the course of chemotaxonomic study of the genus Prunus, seven flavonol glycosides were isolated from the leaves of Prunus serotina Ehrh., characterized by UV and NMR spectroscopy, and identified finally as three quercetin monosides: hyperoside, avicularin, reynoutrin, three quercetin biosides: 3-O-(6"-O-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside, 3-O-(2"-O-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside and 3-O-(2"-O-alpha-L-rhamnopyranosyl)-beta-D-galactopyranoside as well isorhamnetin 3-O-(6"-O-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside. The presence of determined flavonoids in the flowers was confirmed by TLC.     

朝鲜白头翁的三萜皂苷成分研究

目的　研究朝鲜白头翁［Pulsatilla cernua(Thunb.) Bercht. et Opiz.］ 根茎的化学成分。 方法　朝鲜白头翁根茎的乙醇提取物经大孔树脂、硅胶、ODS柱以及HPLC分离得到6个化合物, 通过波谱(1H,¹³CNMR,FAB-MS等)分析和化学方法进行结构鉴定。结果　6个化合物分别被鉴定为pulsatilla saponin A(1), 常春藤皂苷元3-O-β-D-吡喃葡糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖苷(2), pulsatilla saponin D(3), dipsacoside B(4), 3-O-α-L-吡喃鼠李糖(1→2)［β-D-吡喃葡糖(1→4)］-α-L-吡喃阿拉伯糖常春藤皂苷元28-O-β-D-吡喃葡糖酯苷(5)和胡萝卜苷(6)。 结论　5为新化合物, 命名为朝鲜白头翁丙苷(cernuoside C)。 2,4和6为首次从朝鲜白头翁中分离获得。     

鹰爪叶化学成分研究

从鹰爪 Artabotrys hexapetalus 叶分得5种黄酮:鹰爪甙-A(1),鹰爪甙-B(2),黄杉素(3),木犀草素-7-O-葡萄糖甙(4),芹菜素-7-O-芹糖(1→2)葡萄糖甙(5),以及琥珀酸(6)和富马酸(7)。其中1和2是新的黄酮甙,其他5种成分为第一次从本植物分得。经光谱解析测定了1和2化学结构。     

Four new oleanane type saponins from Morina nepalensis var. alba

Four new oleanane type saponins, monepalosides G-J (1-4), were isolated from the water-soluble part of the whole plant of Morina nepalensis var. alba Hand-Mazz. On the basis of chemical and spectroscopic evidence, their structures were determined as 3-O-alpha-L-arabinopyranosyl-(1 --> 3)-alpha-L-arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl-(1 --> 6)-beta-D-glucopyranoside (monepaloside G, 1), 3-O-alpha-L-arabinopyranosyl-(1 --> 3)-beta-D-xylopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl-(1 --> 6)-beta-D-glucopyranoside (monepaloside H, 2), 3-O-alpha-L-arabinopyranosyl-(1 --> 3)-[beta-D-glucopyranosyl-(1 --> 2)]-alpha-L-arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl-(1 --> 6)-beta-D-glucopyranoside (monepaloside I, 3), 3-O-beta-D-glucopyranosyl-(1 --> 4)-beta-D-glucopyranosyl-(1 --> 3)]-alpha-L-arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl-(1 --> 6)-beta-D-glucopyranoside (monepaloside J, 4), respectively. Two-dimensional NMR spectra, including H-H COSY, HMQC, 2D HMQC-TOCSY, HMBC and ROESY were utilized in the structure elucidation and complete assignments of 1H and 13C NMR spectra.     

New lupane glycosides from Pulsatilla chinensis

Two new lupane glycosides along with five known triterpenoids were isolated from the roots of Pulsatilla chinensis (Ranunculaceae). The structures of the new glycosides were determined to be 3-O-beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (pulsatilloside D, 6) and 3-O-[beta-D-glucopyranosyl(1-->4)][alpha-L-rhamnopyranosyl(1-->2)]-alpha-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (pulsatilloside E, 7) by spectroscopic analysis and chemical methods. The compounds were evaluated for cytotoxic activities against K-562 human leukemia and HeLa cells.     

Acylphloroglucinol glycosides from the fruits of Pyracantha fortuneana

Three new acylphloroglucinol glycosides, namely pyrafortunosides A (1), B (2) and C (3), together with three known glycosides (4-6), were isolated from the fruits of Pyracantha fortuneana (Maxim.) Li. Their structures were established to be 2,4,6-trihydroxy-acetophenone-6-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (1), 2,4,6-trihydroxy-benzophenone-6-O-alpha-L-rhamno-pyranosyl-(1-->6)-beta-D-glucopyranoside (2), 2,4,6-trihydroxy-benzophenone-6-O-beta-D-apiofuranosyl-(1-->6)-beta-D-gluco-pyranoside (3), garcimangosone D (4), 2,4,6-trihydroxy-acetophenone-6-O-beta-D-glucopyranoside (5), and 2,4,6-trihydroxy-acetophenone-4-O-beta-D-glucopyranoside (6) by spectral analysis. The three known glycosides (4-6) were obtained from this genus for the first time.     

石胆草中苯乙醇苷的分离与结构鉴定石胆草中苯乙醇苷的分离与结构鉴定

目的 研究石胆草(Corallodiscus flabellata)的化学成分。方法 利用大孔吸附树脂、Sephadex LH-20、硅胶柱色谱进行分离纯化,根据化合物的理化性质和光谱数据鉴定结构。结果分离并鉴定了3个新的苯乙醇苷类化合物,经光谱鉴定化合物的结构为:3,4-二羟基苯乙醇-8-O-［β-D-芹糖基(1→3)］-β-D-葡糖苷(I),3,4-二羟基苯乙醇-8-O-［4-O-咖啡酰基-β-D-芹糖基(1→3)-β-D-葡糖基(1→6)］-β-D-葡糖苷(II),3,4-二羟基苯乙醇-8-O-［β-D-芹糖基(1→3)-β-D-葡糖基(1→6)］-β-D-葡糖苷(III)。结论I,II和III均为新化合物。     

Two new isoflavone glycosides from Pueraria lobata

Two new isoflavone diglycosides, formononetin 8-C-[beta-D-apiofuranosyl-(1 --> 6)]-beta-D-glucopyranoside (1) and formononetin 8-C-[beta-D-xylopyranosyl-(1 --> 6)]-beta-D-glucopyranoside (2), were isolated from the roots of Pueraria lobata, together with four known compounds, 4'-methoxypuerarin (3), daidzin (4), genistin (5), and daidzein (6). The structures of these compounds were elucidated by the spectroscopic methods.