小针刀联合穴位埋线治疗神经根型颈椎病的临床效果观察

观察小针刀联合穴位埋线治疗神经根型颈椎病的临床效果。方法：研究对象选取2020年9月至2021年3月,因颈椎病来我院就诊的患者。采用随机数字法将患者分为两组。一组住院后采取小针刀联合穴位埋线的方法,另外一组入院后采用单独小针刀治疗的方法。 结果：共86 例神经根型颈椎病患者纳入本研究。小针刀联合穴位埋线治疗神经根型颈椎病患者的总有效率为90.7%,单纯针刀治疗组的治疗有效率为83.7%。两组的差异具有统计学意义（P=0.0372）。小针刀联合穴位埋线治疗神经根型颈椎病的痊愈率为67.4%,小针刀组的治愈率为53.5%。小针刀联合穴位埋线治疗神经根型颈椎病在提高痊愈率方面更具优势（P=0.023）。小针刀联合穴位埋线组的患者术后一周后VAS 评分开始低于单独小针刀治疗组患者。在术后1周之内,两组患者的VAS评分没有统计学差异。在术后1个月时,两组的差异很明显（P=0.0165）。结论：小针刀联合穴位埋线方法治疗神经根型颈椎病是一种有效的治疗方法,效果要优于单独小针刀治疗,值得临床推广。     

椎间孔阻滞加按摩牵引治疗颈椎病临床观察

目的观察椎间孔阻滞加按摩牵引治疗颈椎病的疗效。方法选取80例颈椎病患者随机分成2组各40例,Ⅰ组为按摩、牵引法治疗;Ⅱ组为椎间孔阻滞加按摩、牵引。治疗效果分3级:痊愈、显效、无效。結果Ⅰ组治疗后,痊愈率为37．5％(15例),显效率为52．5％(21例),Ⅱ组治疗后,痊愈率为57．5％(23例),显效率为40％(16例),Ⅱ组痊愈率明显高于Ⅰ组。两组间痊愈率差异有统计学意义(P<0．01)。结论颈部椎间孔阻滞加按摩、牵引治疗颈椎病疗效明显优于按摩牵引治疗颈椎病。     

加减葛根桂枝汤结合针刺、牵引治疗神经根型颈椎病63例

目的探寻治疗神经根型颈椎病的较佳疗法。方法将63例神经根型颈椎病患者分为针刺牵引组（21例）、中汤药结合针刺牵引组（22例）、单纯牵引组（20例）。结果中药结合针刺牵引组、针刺牵引组愈显率分别为90．91％、85．71％,优于单纯牵引组的55．00％（P〈0．05）;中汤药结合针刺牵引组的第1疗程痊愈率高于其他两组（P〈0．05）。结论中汤药结合针刺、牵引治疗本病效果好,疗程最短。     

蜂毒注射液治疗颈椎病的临床研究

目的：探讨蜂毒注射液在颈椎病中的治疗作用。方法：选择颈椎患者60例，随机分为对照组和治疗组，每组30例。对照组应用推拿按摩、理疗方案治疗，治疗组用蜂毒注射液穴位注射。结果：两组有效率均为100％，治疗组显效率为（86．67％），对照组为（40％）．两组对比差异有显著性（P〈0．05）。疼痛、麻木、眩晕、压痛点等症状消失时间，治疗组明显短于对照组（P〈0．01）。结论：蜂毒注射液在颈椎病中的治疗中不失为一种有效的治疗方法。     

穴位埋线法对阴虚火旺型失眠证的临床观察研究

[目的]探讨穴位埋线法对阴虚火旺型失眠证的治疗效果。[方法]对120例阴虚火旺型失眠证病例随机分为穴位埋线组、针刺组和药物组（各40例）,以匹兹堡睡眠质量指数量表（PSQI）评分及睡眠率的变化进行疗效评价。[结果]三组均能有效改善患者的失眠状况,虽然随访后有一定的复发,但埋线组和针刺组的维持疗效明显优于药物组。在影响PSOI总分上,三组均有非常明显的改善,埋线组和针刺组的改善效果明显优于药物组。在影响PSQI各项目上,埋线组和针刺组疗效相当,针刺组除入睡时间、睡眠时间项目外均优于药物组（P〈0．05）,而埋线组除睡眠时间外均明显优于药物组（P〈0．01）。随访时的PSQI总分及各项目比较,三组均有不同程度的复发,针刺组除睡眠时间外均优于药物组;而埋线组的维持疗效明显优于药物组。[结论]穴位埋线法治疗阴虚火旺型失眠证是较为安全有效的方法。     

穴位埋线结合腹部按摩治疗中风后便秘的临床观察

目的：观察穴位埋线结合腹部按摩治疗中风后便秘的临床效果。方法：将2013年1-12月在广州中医药大学深圳附属医院住院的100例中风后便秘患者随机分为治疗组和对照组,每组各50例。治疗组采用穴位埋线结合腹部按摩治疗,对照组采用口服麻仁软胶囊治疗,治疗4周后综合评价治疗效果。结果：治疗组治愈38例、好转10例,总有效率96％;对照组治愈25例、好转15例,总有效率80％,两组总有效率比较,差异有统计学意义（ P＜0.05）。结论：穴位埋线结合腹部按摩对于改善中风后患者便秘疗效显著,操作简便,值得推广。     

穴位埋线治疗慢传输型便秘30例疗效观察

目的观察穴位埋线治疗慢传输型便秘的临床疗效。方法采用随机抽样的方法将61例确诊患者分为治疗组30例和对照组31例。治疗组取天枢、大肠腧、上巨虚、足三里穴位进行埋线治疗,对照组口服麻仁滋脾丸治疗。治疗后比较2组疗效。结果治疗组痊愈率（63.33%）显著高于对照组（16.13%）,2组比较差异有高度统计意义（P〈0.01）;治疗组总有效率（93.33%）显蓍高于对照组（74.20%）,2组比较差异有统计意义（P〈0.05）。结论穴位埋线治疗慢传输型便秘疗效确切,操作简便,值得推广。     

舒尔经胶囊配合穴位埋线治疗气滞血瘀型原发性痛经的疗效观察

目的观察舒尔经胶囊配合穴位埋线治疗气滞血瘀型原发性痛经患者疗效。方法 75例气滞血瘀型原发性痛经患者随机分为两组,治疗组35例采用舒尔经胶囊配合穴位埋线治疗,对照组40例给予单纯采用穴位埋线治疗,治疗结束后进行疗效比较。结果治疗组痊愈率优于对照组(P0.05),两组治疗前后痛经症状积分比较,差异有统计学意义(P0.05)。结论舒尔经胶囊配合穴位埋线对气滞血瘀型原发性痛经有良好的疗效。     

穴位埋线治疗单纯性肥胖症的临床研究

[目的】观察穴位埋线疗法对单纯性肥胖症的临床疗效。【方法]将80例单纯性肥胖症患者随机分为两组。治疗组40例采用穴位埋线疗法治疗,对照组40例以常规针刺疗法治疗,两组于治疗3个疗程后评定疗效。【结果】总有效率治疗组为92．5％,对照组为72．5％,2组比较差异有统计学意义（P〈0．05）。治疗组体重及体重指数（BMI）治疗前后比较,差异均有统计学意义（P〈0．01）;与对照组比较差异有统计学意义（P〈0．05）。【结论】穴位埋线减肥对单纯性肥胖症有明显的疗效。     

仰卧拔伸手法在治疗颈型颈椎病中的作用观察

目的：观察应用推拿手法仰卧拔伸法治疗颈型颈椎病的作用。方法选取我院86例颈型颈椎病门诊患者,43例观察组患者采用推拿手法仰卧拔伸法治疗,其余对照组患者单纯应用颈椎牵引法治疗。比较患者颈椎病症状缓解程度及并发症和复发情况。结果观察组痊愈率和总有效率93.1%（40/43）显著高于对照组,观察组并发症和复发情况均好于对照组。结论仰卧拔伸法发挥中医推拿特色,有效达到舒筋活络的目的,对颈型颈椎病病变起到良好纠正作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.