茶多酚对慢性肾功能衰竭患者脂质过氧化损伤的影响

目的：探讨茶多酚在治疗慢性肾功能衰竭（ＣＲＦ）中抗脂质过氧化及改善肾功能的作用。方法：观察３１例ＣＲＦ患者（治疗组）用海南省茶多酚（ＴＰ）治疗前及治疗后３个月的红细胞超氧化物歧化酶（ＳＯＤ）、还原型谷胱甘肽（ＧＳＨ）、血浆丙二醛（ＭＤＡ）,血清总胆固醇（ＴＣ）、甘油三酯（ＴＧ）,血尿素氮（ＢＵＮ）和血肌酐（ＳＣｒ）含量变化。另以未经ＴＰ治疗的２０例ＣＲＦ患者为对照组,并与８９例健康成人的ＳＯＤ、ＧＳＨ和ＭＤＡ作比较。结果：治疗组及对照组ＣＲＦ患者ＳＯＤ活性明显低于健康成人（Ｐ均＜０．０１）,ＭＤＡ及ＧＳＨ高于健康成人（Ｐ均＜０．０５）;治疗后治疗组ＳＯＤ活性明显升高,ＭＤＡ、ＴＣ、ＴＧ、ＢＵＮ和ＳＣｒ均有降低,与对照组比较差异均显著（Ｐ均＜０．０５）。肾功能改善总有效率前者为６７．７４％,后者为３０．００％。结论：ＣＲＦ患者确实存在ＳＯＤ活性降低及脂质过氧化损伤,海南省茶多酚能有效对抗脂质过氧化,提高氧化酶ＳＯＤ活性,改善ＣＲＦ患者肾功能。     

脑出血与一氧化氮,氧化,脂质过氧化的关系

目的：探讨脑出血与一氧化氮，氧化，脂质过氧化的关系及临床意义，方法：检测９８例脑出血患者以及１００例健康人血浆一氧化氮（Ｐ－ＮＯ），维生素Ｃ（Ｐ－ＶＣ），维生素Ｅ（Ｐ－ＶＥ），过氧化脂质（Ｐ－ＬＰＯ）含量，血浆和红细胞超氧化物歧化酶（Ｐ－ＳＯＤ，Ｅ－ＳＯＤ），活性及红细胞过氧化脂质（Ｅ－ＬＰＯ）含量，采用直线回归和相关，逐步回归分析患者病情（临床神经功能缺损程度积分，ＮＤＳ）和颅内血肿量与上述指标     

急性出血性脑血管病并发多脏器功能失常综合征患者血浆？…

目的 探讨血浆内皮素－１（ＥＴ－１）和降钙素基因相关肽（ＣＧＲＰ）在急性出血性脑血管病（ＡＨＣＶＤ）并发多脏器功能失常综合征（ＭＯＤＳ）发病中的作用。方法 采用放射免疫法分别测定２１例ＡＨＣＶＤ合并ＭＯＤＳ患者（ＭＯＤＳ组）、２０例ＡＨＣＶＤ患者（ＡＨＣＶＤ组）及３０例正常人（正常对照组）血浆中ＥＴ－１和ＣＧＲＰ水平。结果：ＭＯＤＳ组及ＡＨＣＶＤ组血浆ＥＴ－１水平明显高于正常对照组（Ｐ均〈０．０１     

急性出血性脑血管病并发多脏器功能失常综合征患者血浆内皮素-1及降钙素基因相关肽水平的观察

目的：探讨血浆内皮素１（ＥＴ１）和降钙素基因相关肽（ＣＧＲＰ）在急性出血性脑血管病（ＡＨＣＶＤ）并发多脏器功能失常综合征（ＭＯＤＳ）发病中的作用。方法：采用放射免疫法分别测定２１例ＡＨＣＶＤ合并ＭＯＤＳ患者（ＭＯＤＳ组）、２０例ＡＨＣＶＤ患者（ＡＨＣＶＤ组）及３０例正常人（正常对照组）血浆中ＥＴ１和ＣＧＲＰ水平。结果：ＭＯＤＳ组及ＡＨＣＶＤ组血浆ＥＴ１水平明显高于正常对照组（Ｐ均＜０．０１），ＭＯＤＳ组ＥＴ１水平又明显高于ＡＨＣＶＤ组（Ｐ＜０．０１）。ＡＨＣＶＤ组血浆ＣＧＲＰ水平高于正常对照组，但无显著性差异（Ｐ＞０．０５）。而ＭＯＤＳ组血浆ＣＧＲＰ水平明显低于正常对照组，ＥＴ１／ＣＧＲＰ（Ｅ／Ｃ）比值明显高于ＡＨＣＶＤ组及正常对照组（Ｐ均＜０．０１）。结论：血浆ＥＴ１水平升高、ＣＧＲＰ水平降低、Ｅ／Ｃ比值严重失衡与ＭＯＤＳ的发生相关；检测血浆ＥＴ１和ＣＧＲＰ水平对评估ＡＨＣＶＤ患者预后有一定意义     

不同剂量紫外线照射对血液抗氧化作用的影响

实验用不同剂量短波紫外线（ＵＶ－Ｃ）照射家兔离体血液，采用化学发光法（ＣＬ）和分光光度法分别测定血浆超氧化物歧化酶（ＳＯＤ）活力和丙二醛（ＭＤＡ）含量。未照射血液血浆ＳＤＯ活力、ＭＤＡ含量分别为９４６．６７±５７．６５Ｕ／ｍｌ、１７．７６±３．３９ｎｍｏｌ／ｍｌ。小剂量（１５秒）照射后ＳＯＤ活力显著高于未照射血液（Ｐ＜０．０１），剂量增加后（３０秒）ＳＯＤ活力下降并接近未照射水平，照射１分钟后活力明显下降（Ｐ＜０．０５），并随短波ＵＶ－Ｃ剂量（３、６分钟）增大下降增多（Ｐ＜０．００１）。较小剂量ＵＶ－Ｃ（１５秒、３０秒、１分钟）照射后ＭＤＡ含量无明显变化，ＵＶ－Ｃ照射３分钟时ＭＤＡ含量开始出现明显升高（Ｐ＜０．０５），并随剂量加大（６分钟）进一步增高（Ｐ＜０．００１）。结果提示小剂量ＵＶ－Ｃ照射能够增强机体抗氧化能力，大剂量照射则使机体抗氧化能力下降，氧化损伤作用增强；并且说明ＵＶ－Ｃ照射所致机体脂质过氧化损伤与抗氧化能力下降，自由基生成增多、消除不足有关     

茶多酚对慢性肾功能衰竭患者脂质过氧化损伤的影响

目的：探讨茶多酚在治疗慢性肾功能衰竭（ＣＲＦ）中抗脂质过氧化及改善肾功能的作用。方法：观察３１例ＣＲＦ患者（治疗组）用海南省茶多酚（ＴＰ）治疗前及治疗后３个月的红细胞超氧化物歧化酶（ＳＯＤ）、还原型谷胱甘肽（ＧＳＨ）、血浆丙二醛（ＭＤＡ）,血清总胆固醇（ＴＣ）、甘油三酯（ＴＧ）、血尿素氮（ＢＵＮ）和血肌酐（ＳＣｒ）含量变化。另以未经ＴＰ治疗的２０例ＣＲＦ患者为对照组,并与８９例健康成人的ＳＯＤ、Ｇ     

黄芪多糖对脑血栓的疗效实验研究

黄芪多糖并临床常规治疗脑血栓患者３８例与一般临床治疗脑血栓患者３０例,分别测血浆粘度ηｐ、纤维蛋白原含量 Ｆｇ、红细胞膜荧光偏振度Ｐ、血浆胆固醇ＴＣ、血浆甘油三脂ＴＧ、过氧化脂质ＬＰＯ、超氧化物歧化酶ＳＯＤ活性等指标。结果显示各组内治疗前,后比较ηｐ、Ｆｇ、ＴＣ、ＴＧ、ＬＰＯ均显著降低（ｐ＜０．０１）而ＳＯＤ活性显著升高（ｐ<０.０１）,两组间比较呈显著差异（ｐ＜０．０１）。表明黄芪多糖能有效地改善微循环,消除自由基,提高红细胞膜流动性及超氧化物歧化酶的活性。     

精神分裂症的脂质过氧化研究

目的：研究精神分裂症病人活性氧自由基发生及脂质过氧化的生成情况。方法：本研究应用超氧化物歧化酶超微量快速测定法、ＤＴＢＮ直接法、二硫双硝基苯甲酸定量测定法和ＴＢＡ法测定了精神分裂症病人４０例（Ⅰ型２３例，Ⅱ型１７例）和健康对照者４０人血中ＳＯＤ，ＧＳＨ－ＰＸ活力，ＧＳＨ和ＬＰＯ含量。结果：精神分裂症血中ＳＯＤ活力明显高于对照组（Ｐ＜０．０５），ＧＳＨ含量极显著低于对照组（Ｐ＜０．０１），而ＧＳＨ－ＰＸ活力、ＬＰＯ含量无明显变化（Ｐ＞０．０５）。其中Ⅱ型病人ＧＳＨ含量降低有更为明显的趋势。结论：对精神分裂症的脂质过氧化研究具有重要含义。     

颅脑损伤与过氧化脂质及红细胞超氧化物歧化酶的关系

测定了３４例颅脑损伤患者伤后４８ｈ内的脑脊液过氧化脂质（ＣＳＦ－ＬＰＯ）、血浆过氧化脂质（Ｐ－ＬＰＯ）和红细胞超氧化物歧化酶（Ｅｒｙ－ＳＯＤＡ）的含量。结果：ＣＳＦ－ＬＰＯ、Ｐ－ＬＰＯ显著高于对照组（Ｐ＜０．０１～０．００１），Ｅｒｙ－ＳＯＤＡ显著低于对照组（Ｐ＜０．０１）；伤情越重，ＣＳＦ－ＬＰＯ值越高；死亡组ＣＳＦ－ＬＰＯ值明显高于生存组。表明：颅脑损伤后颅内脂质过氧化反应剧增，颅脑损伤后ＣＳＦ－ＬＰＯ水平反映了伤情的轻重。因此，作者认为测定ＣＳＦ－ＬＰＯ对伤情的判断及预后估价具有参考价值。     

紫外线照射充氧自血回输对自由基水平的影响

采用紫外线照射充氧自血回输治疗动脉硬化性脑梗塞患者５０例．５天治疗１次，５次为１疗程。测定１疗程前后的红细胞超氧化物歧化酶（ＳＯＤ）活力及血浆过氧化脂质（ＬＰＯ）含量。结果，治疗后脑梗塞患者红细胞ＳＯＤ活力明显增高（Ｐ＜０．００１），血浆ＬＰＯ含量明显下降（Ｐ＜０．００１）。表明该疗法可增强脑梗塞患者清除自由基酶ＳＯＤ活力，调节体内自由基平衡．     

四逆汤抗血管内皮脂质过氧化损伤的作用机制研究

目的比较四逆汤与抗氧化剂维生素E抗血管内皮脂质过氧化损伤作用,旨在探讨四逆汤防治冠心病的可能机制。方法60例老年冠心病心绞痛患者,分别在常规西药异山梨酯的基础上,采用SPSS软件随机分为3组。四逆汤辨证和辨病组各20例、维生素E组20例,观察患者治疗前后血脂代谢及血管内皮功能氧化损伤指标(SOD活性、MDA含量、NO水平、ET浓度)的变化。结果四逆汤能改善缺血ECG和临床症状,对部分血脂代谢指标血清总胆固醇(TC)、血清高密度脂蛋白胆固醇(LDL-C)及血清载脂蛋白A/血清载脂蛋白B(ApoA1/ApoB)有改善作用。辨证组与维生素E组差异有显著性意义(P<0.01)。四逆汤能显著升高血浆SOD活性和NO水平,降低MDA含量,ET-1浓度下降,与维生素E组比较,各项指标差异无显著性意义(P>0.05)。结论四逆汤具有抗血管内皮功能氧化损伤及调节血管内皮细胞功能的作用,其效果与维生素E相似。调节血脂代谢、调节血管内皮细胞功能是四逆汤防治冠心病的可能机制。     

冠心病患者外周血中CD19^＋细胞表达水平的意义

目的观察冠心病患者外周血中CD19^＋的表达水平,并探讨CD19^＋与炎症反应和脂质过氧化反应的关系。方法按美国心脏病学会/美国心脏学会冠心病处理指南的诊断标准并经冠状动脉造影检查确诊的冠心病患者60例,分为稳定型心绞痛（SA）组20例,急性冠状动脉综合征（ACS）组40例;并选择冠状动脉造影正常的20例非冠心病患者作为对照组。应用流式细胞术检测所有研究对象外周血中淋巴细胞表面CD19^＋的表达水平,比较各组CD19^＋的表达情况。同时检测C反应蛋白（CRP）和低密度脂蛋白胆固醇（LDL-C）浓度。并对CD19^＋细胞的表达率与CRP滴度和LDL-C进行Pearson直线相关分析。结果 ACS组患者B淋巴细胞CD19^＋表达率明显高于对照组（10.16±3.63）%vs（4.25±0.96）%（P〈0.01）,亦明显高于SA组（10.16±3.63）%vs（5.39±1.36）%（P〈0.01）;SA组CD19^＋细胞表达率和对照组患者CD19^＋细胞表达率相近（5.39±1.36）%vs（4.25±0.96）%（P〉0.05）。所研究对象外周血中CD19^＋细胞的表达水平与CRP滴度（r=0.661,P〈0.01）和LDL-C（r=0.351,P〈0.01）呈正相关。结论随着冠心病病情加重,冠心病患者外周血中B淋巴细胞CD19^＋的表达水平升高,CD19^＋的表达水平可反映冠心病危险分层,CD19^＋与炎症反应和脂质过氧化反应有关系。     

Oxidative stress and antioxidants in patients with cardiogenic shock complicating acute myocardial infarction

BACKGROUND: Lipid peroxidation and derived oxidized products are being intensively investigated because of their potential to cause injury and because of their pathogenic role in several diseases. The view that an excess of lipid peroxidation products is present and is relevant in the pathogenesis of cardiogenic shock-induced damage has still not received definitive support. METHODS: To evaluate the extent of lipid peroxidation, the status of enzymatic and nonenzymatic antioxidants in patients with cardiogenic shock that complicate acute myocardial infarction (AMI) and to compare with normal subjects. RESULTS: Compared with normal subjects, cardiogenic shock patients had higher malondialdehyde, conjugated dienes and reduced activities of erythrocyte antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lower concentrations of reduced glutathione (GSH) in erythrocyte and in plasma GSH, vitamin C, vitamin E and in beta-carotene. CONCLUSIONS: Cardiogenic shock is associated with greater than normal lipid peroxidation and with an imbalance in antioxidants' status. These results indicate that low activities of SOD, CAT, GPx and low concentrations of GSH, vitamin C, vitamin E and beta-carotene in the circulation of patients with cardiogenic shock complicating AMI may be due to increased utilization to scavenge lipid peroxides. Decrease in plasma concentrations of GSH, vitamin E and beta-carotene seems to be responsible for the elevation of lipid peroxidation in cardiogenic shock complicating AMI compared with MI.     

黄芪及其注射液对冠心病患者胰岛素、血糖和血脂影响的研究

目的研究黄芪对冠心病患者胰岛素、血糖和血脂的影响。方法将106例冠心病患者分为黄芪治疗组(治疗组)和常规治疗组(对照组)。黄芪治疗组在常规治疗的基础上静脉滴注黄芪注射液4周,治疗前后测胰岛素、血糖和血脂。结果黄芪治疗组治疗前后胰岛素敏感指数有显著性差异(1.81±0.64与1.35±0.27,P<0.01)。结论黄芪注射液可改善胰岛素抵抗,对防止有胰岛素抵抗因素在内的疾病有很大的临床意义。     

Relation between features of the psychoemotional status and lipid metabolism of patients with ischemic heart disease and arrhythmias

Interrelationship of the peculiarities of the psychoemotional status, the activity of lipid peroxidation and the content of blood free fatty acids were studied in 64 CHD patients with arrhythmia. It was shown that prolonged and pronounced psychoemotional strain, high levels of the activity of free radical lipid oxidation and the content of nonesterified fatty acids in the blood of CHD patients promoted the development of cardiac rhythm disturbances, particularly of ventricular extrasystoles. Patients with the psychoemotional strain syndrome demonstrated direct correlation of an induced initial hemiluminiscence flash value and the level of a psychological pattern MMPI by scales 2, 6, 7, 0, as well as close direct correlation of the concentration of free fatty acids with scales 6, 8, F and negative correlation with scale 3. The detection of the psychoemotional strain syndrome in CHD patients, especially in combination with high levels of lipid peroxidation and nonesterified fatty acids in the blood, may evidently serve an important criterion for defining patients at high risk of developing arrhythmias.     

黄芪注射液对冠心病患者血糖、胰岛素和血脂的影响

目的观察黄芪对冠心病患者血糖、胰岛素和血脂的影响。方法将 6 4例冠心病患者分为黄芪治疗组 (治疗组 )和常规治疗组 (对照组 )。黄芪治疗组在常规治疗的基础上静脉滴注黄芪注射液 4周 ,治疗前后测血糖、胰岛素和血脂。结果黄芪治疗组治疗前后胰岛素敏感指数有显著性差异 (1.81± 0 .6 4与 1.35± 0 .2 7,P <0 .0 1)。结论黄芪注射液可改善胰岛素抵抗 ,对防止有胰岛素抵抗因素在内的疾病有很大的临床意义。     

Lipid peroxidation and antioxidant defense in patients with severe herpetic infection

Lipid peroxidation and antioxidant defense were studied in 42 patients with severe herpetic infection. The higher plasma and red blood cell levels of intermediate (trienic conjugates) and end (Schiff's bases) lipid peroxidation products were revealed during remission and disease recurrences. At the same time, there were increases in the content of ceruloplasmin in the plasma and in that of superoxide dismutase and catalase in the red blood cells. In the patients with herpetic infection, there were different correlations between the values of lipid peroxidation and antioxidant defense, which were absent in the group of donors. There was a direct correlation between the level of oxidation of intermediate products (ketodienes) of neutral lipids in the plasma and the concentrations of ceruloplasmin and negative correlations with red blood cell superoxide dismutase in patients in remission. There were negative correlations between the red blood cell level of oxidation of intermediate phospholipid peroxidation products and the concentration of ceruloplasmin.     

Free radicals antioxidant enzymes and lipid peroxidation in different types of leukemias

Free radicals are highly reactive species that have been implicated in the pathogenesis of many diseases. Reactive oxygen species can initiate lipid peroxidation and DNA damage leading to mutagenesis, carcinogenesis and cell death, if the antioxidant system is impaired. This study was undertaken to examine the prevalence of oxidative stress and the role of antioxidant defence in untreated leukemia patients. The generation of superoxide anion and hydrogen peroxide by leukocytes, plasma malondialdehyde levels, red cell copper zinc superoxide dismutase (Cu-Zn SOD) and glutathione peroxidase (GSH-PX) activities were determined in 30 patients with different types of leukemias prior to therapy. The superoxide anion generation by polymorphonuclear leukocytes was found to be significantly increased in leukemia patients especially those with acute lymphocytic and nonlymphocytic leukemias, while the hydrogen peroxide levels were comparable to the control values. Plasma lipid peroxidation products in untreated leukemia patients were in the normal range. Red cell Cu-Zn SOD and GSH-PX activities were significantly increased and showed no correlation with the hemoglobin content. Although superoxide generation was high, lipid peroxide levels were normal in these patients. This might be due to the increased activities of the antioxidant enzymes (SOD, GSH-PX) which counteract lipid peroxidation. Increased free radical generation, especially superoxide anion in leukemia patients and increased antioxidant defence enzymes, which is an adaptive protective response, are indicative of mild oxidative stress. There were no significant differences for the parameters cited above between different types of leukemias, suggesting that the changes are not specific to the type of leukemia.     

Effects of vitamin E supplementation on blood antioxidants levels in patients with Behçet’s disease

Behçet’s disease (BD) is known for many years, yet its etiology remains unknown. In BD, the increased production of reactive oxygen species from activated neutrophils may reduce concentrations of antioxidant vitamins and enzymes in plasma and red blood cells (RBC). Vitamin E is an important fat soluble antioxidant and its role on antioxidant parameters of BD is unclear. The study was undertaken to evaluate the role of vitamin E on lipid peroxidation (MDA) levels, antioxidant vitamin and enzyme concentrations in plasma and RBC in patients with BD. There were three groups i.e., control, patient and treatment groups with twenty-five subjects in each. Nonsmoking patients with BD, patient group, was compared with an equal number of healthy control subjects (control group). Blood samples were taken from both control and patient groups and then oral vitamin E was daily supplemented to the patients with BD for six weeks (treated group). At the end of six weeks, blood was taken from the treated group once more.

RBC and plasma MDA levels, serum neopterin, complement system (C₃ and C₄), ASO, CRP, rheumatoid factor, plasma lipoprotein (a), total cholesterol concentrations and erythrocyte sedimentation rate were higher in the patient group than in the control group, but they were lower in the treatment group than in the patient group. While vitamins A, E and β-carotene concentrations in plasma, catalase, glutathione peroxidase activities and reduced glutathione levels in RBC and plasma were lower in patient group than in the controls, they were found to be higher in the treatment group than in the patient group.

These results provide some evidence for a potential role of increased lipid peroxidation and decreased enzymatic and non enzymatic antioxidants in BD by its inflammatory character and vitamin E which may strengthen the antioxidant defense system, and may contribute to the treatment of BD.     

Procedure for determination of oxidative modification of plasma fibrinogen

The purpose of the present study was to develop a new procedure for determining the oxidative modification of plasma fibrinogen. The procedure was developed to use blood plasma from 96 males aged 35-60 years who had cardiovascular diseases: 49 patients with coronary heart disease (CHD), including 16 patients with sub-acute myocardial infarction (MI), and 47 patients with arterial hypertension without CHD. The new procedure is as follows: a rapid fibrin isolating method, a reaction with 2,4-dinitrophenylhydrazine in 2 M HCl solution, by subsequently rinsing in the ethanol : ethyl acetate (1:1) solution, dissolving the precipitate in 8 M urea, and by determining the level of the resultant dinitrophenylhydrazones by spectrophotometry at 363 nm, followed by conversion to the plasma concentration of fibrinogen. The procedure is of informative value for the degree of oxidative fibrinogen modification under oxidative stress; it is technically simple, takes little time, and shows a good reproducibility. The values of determined oxidized plasma fibrinogen by the developed procedure show a high positive correlation with the estimates of oxidized total blood protein fraction and with the values of blood lipid peroxidation. The detected elevated level of oxidized fibrinogen in CHD and MI suggests that this index is a new diagnostic marker of oxidative stress in cardiovascular diseases of atherosclerotic genesis.