Factor de crecimiento de hepatocitos plasmático en preeclampsia y eclampsia

Objective

To compare plasma hepatocyte growth factor concentrations in eclamptic, preeclamptic and normotensive pregnant women.

Materials and method

We included 30 patients with mild preeclampsia (group A), 30 patients with severe preeclampsia (group B) and 30 patients with eclampsia (group C). A control group of 30 healthy pregnant women (group D) was selected with a similar age and body mass index to participants in the study groups. Only nulliparous patients were included. Blood samples were collected for plasma hepatocyte growth factor determination in all patients before delivery and in the study groups immediately after diagnosis.

Results

Plasma hepatocyte growth factor values were highest in eclamptic patients and in severe preeclamptic patients and were lowest in mild preeclamptic patients. Hepatocyte growth factor values were significantly higher in the study groups than in controls (P<.05). Linear regression analysis revealed that 24-hour proteinuria significantly affected plasma hepatocyte growth factor concentrations (P<.05).

Conclusion

The findings of this research showed that plasma hepatocyte growth factor concentrations were higher in eclamptic and preeclamptic patients than in normotensive pregnant women.     

Micropartículas plaquetarias en preeclampsia y eclampsia

Objective

To compare platelet microparticle values in eclamptic, preeclamptic and normotensive pregnant women.

Material and methods

Patients attending the Dr. Urquinaona Central Hospital in Maracaibo, Venezuela, were selected. Thirty patients with mild preeclampsia (group A), 30 with severe preeclampsia (group B) and 30 with eclampsia (group C) were included. Thirty-five healthy women with a similar age and body mass index to those in the study groups were selected as controls (group D). Only nuliparous patients were included. Blood samples were collected before delivery from all patients and immediately after diagnosis for platelet microparticle determination in the study groups.

Results

Statistically significant differences were found in gestational age between groups B and C compared with the control group (P < .05). Higher platelet microparticle values were found in eclamptic patients and severe preeclamptic patients. Lower values were found in mild preeclamptic patients. Significantly higher platelet microparticle values were found in the study groups than in controls (P < .05). When linear regression was performed, the factors significantly affecting platelet microparticle values were 24-hour proteinuria, transaminase and uric acid levels and platelet count (P < .05).

Conclusions

Our findings indicate that platelet microparticle values are higher in eclamptic and preeclamptic women than in normotensive pregnant women.     

Efecto de las hormonas sexuales sobre las concentraciones de homocisteína en preeclámpticas y embarazadas normales

Objective

To establish the effects of sexual hormones on plasma homocysteine concentrations in preeclamptic and normotensive pregnant women.

Methods

There were two groups: group A consisted of 35 preeclamptic patients and group B of 35 normotensive pregnant women used as controls. Blood samples were collected before labor in both grops and immediately after diagnosis in group A. Concentrations of testosterone, free testosterone, dehidroepiandrosterone sulphate, androstenodione, estradiol and homocysteine were measured.

Results

There were statistically significant differences in gestational age, the presence of proteinuria, birthweight and systolic and diastolic blood pressure (p < 0.05). Testosterone, free testosterone and dehidroepiandrosterone sulphate were significantly higher in group A than in group B (P<.05). Estradiol concentrations were significant lower in the group A than in group B (P<.05). Strong positive and significant correlations were found between testosterone, dehidroepiandrosterone sulphate and free testosterone and a moderate negative correlation was found between estradiol and plasma homocysteine concentrations (P<.05).

Conclusions

In preeclamptic and normotensive pregnant women, plasma homocysteine concentrations are positively affected by testosterone, dehidroepiandrosterone sulphate and free testosterone and are negatively affected by estradiol.     

Concentraciones de gonadotropina coriónica humana en pacientes preeclámpticas y embarazadas normotensas sanas

Objective

To compare chorionic gonadotrophin concentrations in preeclamptic and healthy normotensive pregnant women.

Subjects and methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for chorionic gonadotrophin determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (P=NS). Statistically significant differences in chorionic gonadotrophin concentrations were found between patients in group A (47.661+/- 18.124 mUI/mL) and patients in group B (27.459 +/- 13.329 mUI/mL; P<.05). There was a moderate, positive and significant correlation with values of systolic blood pressure (r = 0.493; P<.05) and values of diastolic blood pressure (r = 0.504; P<05).

Conclusions

Chorionic gonadotrophin concentrations were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de interferón gamma en preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of interferon-gamma in patients with preeclampsia and healthy normotensive pregnant women.

Material And Methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for interferon-gamma determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (p=ns). Statistically significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). Interferon-gamma concentrations were significantly higher in group A (75.5±27.7 pg/ml) than in group B (54.2±29.6 pg/ml, p<0.05) and there was a slight, positive and significant correlation with values of systolic blood pressure (r=0.383; p<0.05) and diastolic blood pressure (r=0.259; p<0.05).

Conclusion

Interferon-gamma concentrations were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de interleucina-6 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of interleukin-6 in patients with preeclampsia and healthy normotensive pregnant women.

Material and methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for interleukin-6 were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (p=ns). Statistically significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). No significant differences were found in interleukin-6 concentrations in the group A (31.4±3.5 pg/ml) and patients in group B (25.2±5.7 pg/ml; p<0.05), but a moderate, positive and significant correlation was found with mean systolic (r=0.489; p<0.05) and diastolic (r=0.518; p<0.05) blood pressure.

Conclusions

Interleukin-6 concentrations were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de quimiocina RANTES en preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of chemokine RANTES in pre-eclampsia patients and healthy normotensive pregnant women.

Method

A total of 100 patients were selected. We included 50 pre-eclamptic patients as cases (group A) and a control group of 50 healthy normotensive women with the same age and body mass index as the study group (group B). Blood samples were collected in all patients before labor and immediately after diagnosis in group B to determine RANTES concentrations.

Results

There were no significant differences in maternal age, gestational age, or body mass index when the samples were taken (P = ns). RANTES concentrations showed statistically significant differences between cases (group A; 2484.6 ± 113.7 pg/mL) and controls (group B; 2002.8 ± 62.6 pg/mL; P < .05). There was a moderate, positive and significant correlation with systolic blood pressure values (r = .283; P < .05) and with diastolic blood pressure values (r = .322; P < .05).

Conclusions

Chemokine RANTES concentrations were significantly higher in pre-eclampsic patients than in healthy normotensive pregnant women.     

Concentraciones de interleucina 13 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare interleukin-13 concentrations in patients with preeclampsia and healthy normotensive pregnant women.

Material and methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for interleukin-13 determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (p=ns). Statistically significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). No significant differences were found in interleukin-13 concentrations in the group A (40.1±1.1 pg/ml) and patients in group B (48.1±1.5 pg/ml; p<0.05), but a strong, positive and significant correlation was found with mean systolic (r=0.866; p<0.05) and diastolic (r=0.839; p<0.05) blood pressure.

Conclusions

Interleukin-13 concentrations were significantly lower in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de interleucina-8 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of interleukin-8 in patients with preeclampsia and healthy normotensive pregnant women.

Material and methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for interleukin-8 were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (p=ns). Statistically significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). Interleukin-8 concentrations were significantly higher in group A (54.8±13.6 pg/ml) than in group B (33.6±6.3 pg/ml, p<0.05) and there was a strong, positive and significant correlation with values of systolic blood pressure (r=0.715; p<0.05) and diastolic blood pressure (r=0.640; p<0.05).

Conclusion

Interleukin-8 concentrations were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.     

Homocisteína sérica en neonatos de preeclámpticas y de embarazadas normotensas

Objective

To compare serum homocysteine concentrations in the newborns of preeclamptic patients and normotensive pregnant women.

Methods

Thirty-five preeclamptic patients and their newborns (group A; cases) and 35 normotensive pregnant women and their newborns (group B; controls) were selected. Maternal-neonatal homocysteine concentrations and neonatal weight were evaluated in both groups.

Results

Statistically significant differences were found in gestational age, systolic and diastolic blood pressure and the presence of proteinuria (p<0.05). Maternal homocysteine concentrations were higher in group A than in group B (p<0.05). Mean neonatal weight was significantly lower in group A than in group B (p<0.05). Neonatal homocysteine concentrations were higher in group A than in group B (p<0.05). When the homocysteine concentrations of newborns were correlated, there was a moderate, positive and significant correlation with maternal homocysteine concentrations and a moderate, negative and significant correlation with neonatal weight (p<0.05).

Conclusion

Homocysteine concentrations were significantly higher in the newborns of preeclamptic patients than in those of normotensive pregnant women.     

Concentraciones de interleucina-2 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare interleukin-2 concentrations in patients with preeclampsia and healthy normotensive pregnant women.

Material And Methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for interleukin-2 determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (p=ns). Statistically significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). No significant differences were found in interleukin-2 concentrations in the study group (68.7±29.6 pg/ml) and patients in the control group (59.9±18.4 pg/ml), but a slight, positive and significant correlation was found with mean systolic blood pressure (r=0.227; p<0.05).

Conclusions

Interleukin-2 concentrations were similar in preeclamptic patients and healthy normotensive pregnant women.     

Concentraciones de interleucina 4 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of interleukin-4 in patients with preeclampsia and healthy normotensive pregnant women.

Material and methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to those in the study group were selected as controls (group B). Blood samples for interleukin-4 determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal or gestational age or body mass index at sample collection (p=ns). Significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). Statistically significant differences were also found in interleukin-4 concentrations in patients in group A (10.0±2.4 pg/ml) and those in group B (13.2±1.5 pg/ml; p<0.05) and a moderate, negative and significant correlation was found with systolic (r=−0.574; p<0.05) and diastolic blood pressure (r=−0.541; p<0.05).

Conclusion

Interleukin-4 concentrations of were significantly lower in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de neuroquinina B como marcador de riesgo en pacientes con preeclampsia

Objective

To establish the usefulness of neurokinin B concentrations as a risk marker in patients with preeclampsia.

Method

A total of 100 patients were selected. Fifty patients with preeclampsia were selected as cases (group A) and 50 healthy normotensive pregnant women with a similar age and body mass index to the study group were selected as controls (group B). Blood samples were collected in all patients before labor and immediately after diagnosis in group B to determine neurokinin B concentrations.

Results

Statistically significant differences in neurokinin B concentrations were found between group A (16.3 ± 9.6 ng/L) and group B (3.5 ± 2.0 ng/L; P<.05). There was a positive and significant correlation with values of systolic and diastolic blood pressure (P<.05). A cut-off value of 5 pg/ml had a value under the curve of 0.93, sensitivity of 75.0%, specificity of 87.5%, a positive predictive value of 90.0%, a negative predictive value of 70.0%, and a diagnostic accuracy of 81.2%.

Conclusions

Concentrations of neurokinin B were significantly higher in patients with preeclampsia than in healthy normotensive pregnant women. This peptide is useful as a risk marker in patients with preeclampsia.     

Selectinas solubles en preeclámpsicas y embarazadas normotensas sanas

Objective

To compare concentrations of soluble selectins in preeclamptic patients and healthy normotensive pregnant women.

Method

A total of 100 patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy normotensive pregnant women with the same age and body mass index as the study group were selected as controls (group B). Blood samples were extracted from all patients before labor and immediately after diagnosis in group A to determine concentrations of P-, E- and L-selectin.

Results

No significant differences were found in maternal age, gestational age or body mass index at sample extraction (P = ns). Significantly higher concentrations of P- and E-selectin were found in group A than in group B (P < 0.05). However, L-selectin concentrations were significantly lower in preeclamptic patients than in normotensive pregnant controls (P < 0.05). A strong, positive and significant correlation was found between P- and E-selectin and values of systolic and diastolic blood pressure (P < 0.05) and a strong, negative and significant correlation was observed between L-selectin concentrations and values of systolic and diastolic blood pressure (P < 0.05).

Conclusions

Preeclamptic patients had significantly higher concentrations of P- and E-selectin and lower concentrations of L-selectin than healthy normotensive pregnant women.     

Concentraciones de interleucina-16 en pacientes con preeclampsia y gestantes normotensas sanas

Objective

To compare interleukin-16 concentrations in patients with preeclampsia and healthy normotensive pregnant women.

Method

A total of 100 patients were selected. Fifty patients with preeclampsia were selected as the study group (group A) and 50 healthy normotensive pregnant women with the same age and body mass index as the study group were selected as controls (group B). Blood samples were extracted from all patients before labor and immediately after diagnosis in group B to determine interleukin-16 concentrations.

Results

There was statistically significant difference in interleukin-16 concentrations between group A (211.9 ± 78.7 pg/ml) and group B (83.6 ± 9.9 pg/ml; p < 0.05). There was a strong, positive and significant correlation with systolic blood pressure values (r = 0.282; p < 0.05) and with diastolic blood pressure values (r = 0.320; p < 0.05). A cutoff value of 180 pg/ml had an area under the curve of 0.95, sensitivity of 94.0%, specificity of 70.0%, a positive predictive value of 75.8% and a negative predictive value of 92.1%, with a diagnostic accuracy of 75.0%.

Conclusions

Interleukin-16 concentrations were significantly higher in patients with preeclampsia than in healthy normotensive pregnant women.     

Proteína C reactiva y homocisteína en menopáusicas tratadas con tibolona

Objective

To determine modifications in C-reactive protein and homocysteine in postmenopausal women who used tibolone.

Materials and methods

We selected a sample of 45 postmenopausal women treated with a 2.5-mg dose of tibolone daily for 6 months. Serum concentrations of C-reactive protein and homocysteine were measured.

Results

C-reactive protein concentrations were significantly increased after 6 months of treatment (0.51 ± 0.29 mg/dl at baseline compared with 0.95 ± 0.34 mg/dl after treatment; P<.05). Homocysteine concentrations were significantly reduced after 6 months of treatment (10.16 ± 1.45 picomol/L) compared with initial values (10.95 ± 1.87 picomol/L; P<.05).

Conclusions

After 6 months of use, tibolone significantly increased C-reactive protein concentrations and reduced homocysteine concentrations.     

Concentraciones del factor de necrosis tumoral alfa en preeclámpticas y embarazadas normotensas sanas

Objective

To compare tumor necrosis factor-alpha concentrations in patients with preeclampsia and healthy normotensive pregnant women.

Material And Methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to patients in group A were selected as controls (group B). Blood samples for tumor necrosis factor-alpha determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample collection (p=ns). Statistically significant differences were found between groups in mean values of systolic and diastolic blood pressure (p < 0.05). Significant differences were found in tumor necrosis factor-alpha concentrations in group A (9.7±4.6 pg/ml) and patients in group B (5.5±1.6 pg/ml; p < 0.05), and a moderate, positive and significant correlation was found with mean systolic (r=0.433; p < 0.05) and diastolic (r=0.412; p < 0.05) blood pressure.

Conclusions

Tumor necrosis factor-alpha concentrations were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de interleucina 10 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of interleukin-10 in patients with preeclampsia and healthy normotensive pregnant women.

Material and methods

One hundred patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 healthy pregnant women with a similar age and body mass index to those in the study group were selected as controls (group B). Blood samples for interleukin-10 determination were collected in all patients before labor and immediately after diagnosis in the study group.

Results

There were no significant differences in maternal or gestational age or body mass index at sample collection (p=ns). Significant differences were found between groups in mean values of systolic and diastolic blood pressure (p<0.05). Statistically significant differences were also found in interleukin-10 concentrations in patients in group A (15.9±3.1 pg/ml) and patients in group B (90.6±20.0 pg/ml p<0.05) and a strong, negative and significant correlation was found with systolic (r=−0.823; p<0.05) and diastolic blood pressure (r=−0.825; p<0.05).

Conclusion

Interleukin-10 concentrations of were significantly lower in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de interleucina-17 en pacientes preeclámpticas y embarazadas normotensas sanas

Objective

To compare concentrations of interleukin-17 in preeclamptic and healthy normotensive pregnant women.

Method

A total of 100 patients were selected. Fifty preeclamptic patients were selected as the study group (group A) and 50 healthy normotensive pregnant women, with a similar age and body mass index as the study group, were selected as controls (group B). To determine interleukin-17 concentrations, blood samples were collected in all patients before labor and immediately after diagnosis in group A.

Results

There were no significant differences in relation to maternal age, gestational age or body mass index at sample extraction (p = ns). Statistically significant differences were found in interleukin-17 concentrations between patients in the study group (group A; 6.0 +/- 0.9 pg/ml) and patients in the control group (group B; 3.9 +/- 1.0 pg/ml; p < 0.05). There was a significant correlation with values of systolic blood pressure (r = 0.232; p < 0.05) and diastolic blood pressure (r = 0.181; p < 0.05) in group A.

Conclusions

Interleukin-17 values were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.     

Concentraciones de interleucina-15 en preeclámpticas y embarazadas normotensas sanas

Objective

To compare interleukin-15 concentrations in preeclamptic patients and healthy normotensive pregnant women.

Method

A total of 100 patients were selected. Fifty preeclamptic patients were selected as cases (group A) and 50 normotensive pregnant women with a similar age and body mass index to the study group were selected as controls (group B). Blood samples were collected before labor in all patients and immediately after diagnosis in group B to determine interleukin-15 concentrations.

Results

There were no significant differences in maternal age, gestational age or body mass index at sample extraction (p = ns). Interleukin-15 concentrations were significantly higher in patients in the study group (group A; 3.21 ± 0.79 pg/ml) than in those in the control group (group B; 2.26 ± 0.24 pg/ml; p < 0.05). There was a moderate, positive and significant correlation with systolic blood pressure values (r = 0.584; p < 0.05) and diastolic blood pressure values (r = 0.589; p < 0.05).

Conclusions

Interleukin-15 concentrations were significantly higher in preeclamptic patients than in healthy normotensive pregnant women.