共查询到16条相似文献,搜索用时 93 毫秒
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中低压制备色谱制备新绿原酸对照品的研究 总被引:1,自引:0,他引:1
目的:建立从金银花中分离制备新绿原酸对照品的方法。方法:利用HPD200A大孔树脂对金银花水提液中的新绿原酸富集,经中低压制备色谱分离制备金银花中新绿原酸单体,运用HPLC对新绿原酸进行含量测定。采用现代波谱技术ESI-MS、1H-NMR、13C-NMR进行结构鉴定。结果:新绿原酸的最佳纯化工艺为加5 BV水洗脱,收集水洗液,浓缩、干燥,得新绿原酸粗品;以乙腈-0.5%甲酸溶液(10:90)为流动相,流速20 mL?min-1,检测波长326 nm,进行分离纯化,分离制备的新绿原酸的纯度达98.86%,收率为89.1%。结论:该方法可有效制备高纯度的新绿原酸对照品,以用于中药新药中定性鉴别和含量测定。 相似文献
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目的建立毛鸡骨草中异夏佛塔苷对照品的制备分离方法。方法取毛鸡骨草药材醇提浸膏的正丁醇萃取部分用硅胶柱色谱分离,氯仿-甲醇(90∶10~70∶30)梯度洗脱,收集异夏佛塔苷富集流份,继续采用低压C18柱色谱及制备HPLC进行分离纯化,通过NMR、MS等波谱方法鉴定化合物结构,经TLC、HPLC-UV及MS联用等技术进行质量分数检测。结果从毛鸡骨草中制备分离的异夏佛塔苷对照品,质量分数99%。结论本法得到的异夏佛塔苷对照品符合中药化学对照品的相关技术要求,为药材毛鸡骨草和含毛鸡骨草的成方制剂的质量控制及药效物质基础研究提供化学对照品。 相似文献
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目的优选超临界CO2萃取蛇床子中蛇床子素工艺,建立制备型高效液相色谱(PHPLC)分离纯化萃取物中蛇床子素的方法。方法以总萃取物得率和萃取物中蛇床子素的量为指标,选取温度、压力和提取次数3个因素,按L9(34)正交设计优选蛇床子素超临界CO2萃取工艺。萃取物经甲醇超声溶解后,采用PHPLC法,色谱柱为:Zorbax SB-C18柱(250 mm×21.2 mm,7μm),流动相为甲醇-水(70∶30),柱温为25℃,体积流量为15 mL/min,检测波长为322 nm,进样量为5 mL,收集蛇床子素馏份,经真空冷冻干燥得蛇床子素单体。结果最佳萃取工艺为:温度50℃,压力25 MPa,提取3次(每次1 h),PHPLC法制备的蛇床子素用面积归一化法与外标法定量,质量分数为98.8%,1H-NMR鉴定结构,与文献数据一致。结论超临界CO2萃取蛇床子中蛇床子素提取效果好,工艺简单可行,PHPLC法制备高纯度蛇床子素,简便快捷,并适于大规模制备蛇床子素。 相似文献
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Jehyeon Ra Sanggon Lee Hye Jin Kim Young Pyo Jang Hyunjong Ahn Jinju Kim 《Journal of ethnopharmacology》2010
Ethnopharmacological relevance
Bambusae Caulis in Taeniam (BC) was known as traditional herbal medicine with anti-inflammatory property in the Orient.Aim of the study
Allergic asthma is inflammatory disease of airways associated with enhanced T helper (Th) 2 lymphocytes responses to allergens, leading to eosinophilic infiltration and elevated serum IgE levels. Although there were some studies that BC extract had an anti-inflammatory property, there was no study on asthma. In present study, we investigated the suitability of BC extract as a therapeutic candidate in the treatment of allergic airway disease in ovalbumin-induced asthma model.Materials and methods
Balb/C mice (female, 6 weeks old) were treated by ovalbumin sensitization and nebulization, and used as asthma model. The number of eosinophil in bronchoalveolar lavage (BAL) fluid and the degree of eosinophila were investigated by hematoxylin and eosin stain and the infiltration of inflammatory cells into lung tissues was examined by staining by hematoxylin and eosin solution. The levels of interleukin (IL)-4 in BAL fluid, immunoglobulin E (IgE) in serum, interferon (IFN)-γ and IL-4 production in splenocyte culture from Balb/C mice (not treated, 6 weeks old) that incubated with or without BC extract for 48 h were determined by enzyme-linked immunosorbent assay.Results
The level of eosinophils was decreased by treatment of the animals with BC extract (40 mg/kg) and correspondingly, a significantly lowered degree of eosinophila was also reported (p < 0.01). In lung tissue, BC extract reduced the increased immune cell infiltration induced by OVA (p < 0.05). Furthermore, the levels of IL-4 and IgE in BAL fluid or serum up-regulated by OVA was decreased by BC extract. Finally, IFN-γ production was significantly increased (p < 0.01), while IL-4 production significantly decreased (p < 0.01), after treatment of the culture supernatants of splenocytes with BC extract.Conclusions
These results indicated that BC extract reduces OVA-induced airway inflammation and Th 2 response in mice, suggesting that BC extract can be a therapeutic candidate for allergic airway disease, including asthma. 相似文献12.
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鸡血藤高效液相色谱指纹图谱研究 总被引:8,自引:3,他引:8
目的 建立鸡血藤的高效液相色谱指纹图谱,以评价鸡血藤的质量。方法 用HPLC法获得鸡血藤药材的色谱图,再用聚类分析方法进行考察。结果 鸡血藤共有11个峰,其中非共有峰6个,共有峰5个,共有峰的相对保留时间分别为:0.046,0.664,1.000,1.198,1.383;相对峰面积分别为:0.819,0.221,1.000,0.331,0.458。鸡血藤及其混淆品种的色谱指纹图谱存在明显差异,所提取的11个色谱峰可作为指纹特征。结论 HPLC色谱指纹图谱分析技术可用于鸡血藤药材的鉴别。 相似文献
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HPLC法测定丁公藤中总东莨菪内酯含量 总被引:13,自引:1,他引:13
目的:为确定丁公藤药材质控指标积累数据。方法:CLC-ODS1柱,流动相0.5%冰醋酸甲醇溶液水(26∶55),检测波长310nm,反相高效液相色谱法测定总东莨菪内酯含量。结果:平均回收率为99.37%,RSD0.39%。结论:提供了3个品种14批测定数据,其含量限度不低于30mg/100g。 相似文献
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目的:建立野木瓜中木犀草素的含量测定方法.方法:Dikma Diamonsil C18色谱柱(4.6 mm × 250 mm,5μm),流动相乙睛-0.1%磷酸溶液(26∶ 74),流速1 mL·min-,检测波长350 nm,柱温室温.结果:线性范围0.067 75 ~ 2.168 μg(r=0.999 9),平均回收率为99.25%.RSD 1.65%,精密度RSD 2.22%,重复性RSD 1.24%,稳定性RSD 2.58%.结论:方法稳定、可靠,可作为该药材的质量控制方法. 相似文献
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目的测定青风藤植物不同部位中青藤碱的含量,为合理有效地开发青风藤中药资源提供依据。方法采用高效液相色谱法,以Diamonsil-C18(4.6×250mm,5μm)为色谱柱,甲醇-水(50:50)为流动相,检测波长为262nm,流速:1.0ml/min;进样量10μl条件下测定。结果青藤碱在0.27μg~13.50μg范围内呈良好的线性关系,平均回收率为99.46%,RSD值为1.66%;青风藤根中青藤碱的平均含量为1.65%,藤茎皮为1.35%,茎为0.28%,叶为0.11%。结论不同部位青风藤中青藤碱的含量具有较大差异,其中根中最高,藤茎皮次之,茎和叶中较低。 相似文献