慢性肝病患者血清IL-6,IL-8,IL-10含量变化

本文通过检测72例慢性肝病患者血清IL-6、IL-8、IL-10的含量变化,以探讨其在慢性肝病的早期诊断和疗效观察的意义,现将结果报告如下。     

冠心病患者TNF-α,IL-1β,IL-6,IL-8的变化研究

目的 :对冠心病患者血清中肿瘤坏死因子 (TNF -α)、白介素 - 1β(IL - 1β)、白介素 - 6 (IL - 6 )、白介素 - 8(IL - 8)含量进行分析 ,以探讨它们在冠心病发病过程中的意义。方法 :研究对象为正常对照组 36例 ,稳定型心绞痛组 32例 ,心肌梗塞组 39例 ,采用化学发光酶分析法检测其血清TNF -α、IL - 1β、IL - 6、IL - 8水平。结果 :与正常组比较 ,冠心病患者中TNF -α、IL - 1β、IL - 6、IL - 8水平均有不同程度升高 ,尤以心肌梗塞组升高明显 ,其差别有显著临床意义。心绞痛组TNF -α(p <0 0 5 ) ,IL - 6 (p <0 0 1) ,IL - 8(p <0 0 5 )。心肌梗塞组TNF -α(p<0 0 1) ,IL - 1β(p<0 0 5 ) ,IL - 6 (p <0 0 0 1) ,IL - 8(p <0 0 0 1)。 结论 :TNF -α、IL - 1β、IL - 6、IL- 8与动脉粥样硬化和冠心病的发生有密切关系 ,这些细胞因子可通过相互诱导、相互协同共同参与冠心病的发生、发展过程     

严重创伤患者血浆TNF、IL-6、IL-8和内毒素的变化

本研究选择１７例严重创伤患者，根据ＩＳＳ计分将患者分为两组（即ＩＳＳ１６－２５（Ⅰ组）和ＩＳＳ＞２５组（Ⅱ组）），动态观察伤后两周内血浆ＴＮＦ、ＩＬ－６和ＩＬ－８及内毒素水平的变化，并分析其相关性。研究结果显示，创伤后，患者血浆细胞因子水平可相继明显升高，其中血浆ＴＮＦ水平升高较早，血浆ＩＬ－６、ＩＬ－８较晚，Ⅱ组血浆细胞因子水平明显高于Ⅰ组，且三种血浆细胞因子均值分别与ＩＳＳ计分呈显著正相关。血浆内毒素水平在创伤早期也明显升高，其均值分别与ＩＳＳ计分和血浆细胞因子水平也呈显著正相关。研究结果提示，严重创伤能引起明显的细胞因子反应及内毒素血症，创伤后早期内毒素大量侵入可能是创伤后细胞因子产生、释放增加的重要机制之一。     

原发性肝癌患者IL-6、IL-8和TNF水平的观察

白细胞介素 -６（ＩＬ -６）、白细胞介素 -８（ＩＬ-８）和肿瘤坏死因子（ＴＮＦ）为一组多功能细胞因子 ,在抗肿瘤免疫和炎症反应中起着十分重要的作用［１］。细胞因子网络及其受体之间的调节失常常与免疫性疾病和肿瘤的发生有关［２］。本文报道原发性肝癌患者血清中这三种细胞因子水平的相关研究 ,现报告如下。对象和方法一、对象 :（一）正常人 :３５人。均为我院预防保健科体检合格的健康人 ,心、肝、肺、肾等重要脏器无疾患 ,经临床检查除外炎症、免疫性疾病和肿瘤 ,肝、肾功能试验正常。（二）病人组 :３３人。均经临床明确诊断的…     

甲亢、甲减患者血清TNF、IL-6和IL-8水平观察

本文对甲亢与甲减患者进行血清中ＴＮＦ(肿瘤坏死因子 )、ＩＬ - 6 (白细胞介素 - 6 )和ＩＬ - 8(白细胞介素 - 8)的测定 ,从而探讨自身免疫性甲状腺疾病的发生、发展与细胞因子的内在联系。材料和方法一、对象 :甲状腺机能亢进组 37例 (男 17,女 2 0 ) ,年龄 13～ 6 1岁 ;甲状腺机能减退组 32例 (男 10 ,女 2 2 ) ,年龄 2 2～ 5 5岁 ;正常对照组 35例 (男 15 ,女 2 0 ) ,年龄 19～ 4 5岁 ,均为身体健康 ,无甲状腺疾患及其它脏器病变。二、方法 :(一 )受试者清晨空腹采取静脉血 ,立即分离血清 ,置 -2 0℃保存 ,待一次检测。(二 )ＴＮＦ、Ｉ…     

过敏性紫癜患儿血清sIL-2R、IL-6、IL-8 及凝血功能的变化

目的　探讨过敏性紫癜 (HSP)患儿血清可溶性白介素 2受体 (sIL - 2R)、白介素 6 (IL - 6 )、白介素 8(IL - 8)及部分凝血活酶时间 (APTT)、凝血酶时间 (TT)、D -D二聚体在发病过程中的变化。方法　应用化学发光法检测sIL - 2R、IL -6、IL - 8水平 ,用SymexCA - 15 0 0全自动血凝分析仪测定APTT、TT及D -D二聚体。结果　HSP患儿急性期血清sIL - 2R、IL- 6、IL - 8水平明显高于正常 (P <0 0 5 ) ,3个月后恢复至正常对照组水平 (P >0 0 5 )。早期血液呈高凝状态 ,APTT、TT缩短 ,伴继发性纤溶 ,D -D二聚体增高 ,且混合型增高更显著。结论　动态检测HSP患儿血清sIL - 2R、IL - 6、IL - 8及APTT、TT、D -D二聚体的变化 ,为临床判断病情 ,估计预后及抗凝治疗提供理论依据。     

高血压对急性心肌梗死患者血清IL-1β、IL-6及IL-8水平的影响

为探讨高血压对急性心肌梗死（AMI）患者血清白介素-1β（IL-1β）、白介素-6（IL-6）及白介素-8（IL-8）的影响。AMI患者67例,根据高血压病史分为非高血压组37例,高血压组30例,对照组28名,采用ELISA测定各组患者入院第24h、第14d及对照组的血清IL-1β、IL-6及IL-8水平。结果表明非高血压组患者的炎性因子水平于AMI发病第24h显著高于对照组（P〈0．001）,发病第14天炎症因子水平与对照组相比无显著性差异,高血压组患者AMI发病第24h、第14d血清炎症因子水平均显著高于非高血压组和对照组（P〈0．001）。结果显示,IL-1β、IL-6及IL-8参与了AMI的发病,但高血压对炎性因子的升高起到了关键作用。     

孟鲁司特对支气管哮喘患者血清IL-6、IL-8和IL-10水平的影响

目的:探讨孟鲁司特在支气管哮喘患者体内IL-6、IL-8和IL-10水平的影响。方法:应用放射免疫分析和酶联法对31例支气管哮喘患者应用孟鲁司特治疗前后血清IL-6、IL-8和IL-10水平的变化,并与35名正常健康人作比较。结果:支气管哮喘患者在治疗前血清IL-6、IL-8水平非常显著地高于正常人组(P<0.01),而IL-10水平显著地低于正常人组(P<0.01),经治疗2周后与正常人组比较仍有显著性差异(P<0.05)。结论:孟鲁司特对支气管哮喘患者血清IL-6、IL-8和IL-10有一定程度的调节作用,从而降低患者体内的炎症水平,促进病情缓解和好转。     

慢性乙型肝炎、肝硬化、肝癌患者血清IL-8、IL-10和leptin水平变化及其临床意义

目的:探讨慢性乙型肝炎、肝硬化、肝癌患者血清IL-8、IL-10和leptin水平的变化及其临床意义。方法:应用放射免疫分析测定35例慢性乙型肝炎、30例肝硬化、27例肝癌患者的血清IL-8、IL-10和leptin水平,并与正常对照组比较。结果:各组患者的血清IL-8、IL-10和leptin水平均较正常对照组显著地增高,相关分析的数据表明,血清leptin水平与IL-8呈正相关。结论:血清IL-8、IL-10和leptin水平的变化与慢性乙型肝炎、肝硬化、肝癌发生发展密切相关。     

体外循环瓣膜置换术时IL-6、IL-8、IL-10水平的变化

目的:观察体外循环心内直视手术中及术后促炎因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和抗炎因子白细胞介素-10(IL-10)水平的变化。方法:随机选择12例择期行瓣膜置换术、心功能II~IV级的风湿性心脏病患者,分别于下列9个时点采取混合静脉血7mL:麻醉诱导前、诱导后30min、主A阻断后30min、主A开放后10min、主A开放后30min、体外循环(CPB)后30min、CPB后8h、术后24h、术后72h。以3000r／min转速离心10min后留取上清液,酶联免疫吸附反应(ELISA)技术测定细胞因子IL-6、IL-8及IL-10水平。术中输入晶胶体液及血管活性药物维持血流动力学稳定。结果:自主A开放后30min-术后72hIL-6、IL-8水平显著升高,抗炎因子IL-10水平自主A阻断后30min-术后72h也升高。各参数的峰值都位于CPB结束后30min。结论:体外循环瓣膜置换术可使血中IL-6、IL-8和IL-10水平产生明显变化,这些变化于CPB后30min达到高峰,并持续至术后数日。     

蓖麻毒素诱导U937细胞分泌IL-6和IL-8的作用

目的：进一步研究蓖麻毒素是否具有诱生Ｕ９３７细胞分泌细胞因子作用。方法：采用ＭＴＴ法检测了蓖麻毒素对Ｕ９３７细胞的毒性，并采用ＥＬＩＳＡ法测定细胞２上清中的Ｉ－６和ＩＬ－８。结果：蓖麻毒素对Ｕ９３７细胞的生具有时间效应及剂量效应。随着作用时间的延长。诱生２种细胞因子的量逐渐增加；随蓖麻毒素剂量的增加，诱生２种细胞因子的量均减少。结论：蓖麻毒素诱导２种细胞因子的产生为其抗癌应用或其它作用提供理论依据     

IL-2 and IL-10 serum levels in HIV-1-infected patients with or without active antiretroviral therapy

We analyzed IL-2 and IL-10 serum levels in 26 HIV-1-infected patients naive of antiretroviral treatment and in 34 patients receiving highly active antiretroviral therapy (HAART). All patients without treatment were asymptomatic. When they were stratified according to levels of CD4+ T cells, IL-2 levels were significantly increased in patients with > or =200 CD4+/microl and IL-10 levels were significantly increased in patients with <200 CD4+/microl compared to controls. A significant negative correlation was observed between IL10 levels and CD4+ T-cell counts. No correlation was observed between IL-2 and IL-10 levels and viral load due to the wide range of variability in the number of HIV copies/ml present in the different patients. However, IL-2 levels were higher in patients with high viral load than in patients with low viral load. In patients with HAART, IL-2 and IL-10 levels were similar to the control group and no differences were detected respecting CD4+ T cells counts and viral load. Our findings show that the modifications in IL-2 and IL-10 serum levels in HIV-1-infected patients naive of antiretroviral treatment are associated with the progression of immunological damage. Furthermore, they show a dysbalance of type-1/type-2 cytokines with an involvement of type-2 cytokines in later stages of HIV infection. Cytokine dysregulation can be reversed by HAART in the context of immune restoration and viral suppression.     

Dysregulation of IL-2 and IL-8 production in circulating T lymphocytes from young cystic fibrosis patients

It is well documented that patients with cystic fibrosis (CF) are unable to clear persistent airway infections in spite of strong local inflammation, suggesting a dysregulation of immunity in CF. We and others have reported previously that T lymphocytes may play a prominent role in this immune imbalance. In the present work, we compared the reactivity of CD3+ T cells obtained from young CF patients in stable clinical conditions (n = 10, aged 9-16.5 years) to age-matched healthy subjects (n = 6, aged 9-13.5 years). Intracellular levels of interferon (IFN)-gamma, interleukin (IL)-2, IL-8 and IL-10 were determined by flow cytometry after whole blood culture. The data identified T lymphocyte subsets producing either low levels (M1) or high levels (M2) of cytokine under steady-state conditions. We found that the production of IFN-gamma and IL-10 by T lymphocytes was similar between young CF patients and healthy subjects. In contrast, after 4 h of activation with PMA and ionomycin, the percentage of T cells producing high levels of IL-2 (M2) was greater in CF patients (P = 0.02). Moreover, T cells from CF patients produced lower levels of IL-8, before and after activation (P = 0.007). We conclude that a systemic immune imbalance is present in young CF patients, even when clinically stable. This disorder is characterized by the capability of circulating T lymphocytes to produce low levels of IL-8 and by the emergence of more numerous T cells producing high levels of IL-2. This imbalance may contribute to immune dysregulation in CF.     

Variations in cervical IL-10 and IL-8 concentrations throughout gestation in normal pregnancies

PROBLEM: Data regarding cervical interleukin 18 (IL-8) and IL-10 concentrations during pregnancy is limited. METHOD OF STUDY: This was a cross sectional study of healthy pregnant women. Specimens were collected from the cervical os secretions. IL-8 and IL-10 levels were measured by using enzyme-linked immunosorbent assay. Median (range) cytokine concentrations were derived for each trimester and compared across trimesters. The relationship between gestational age and cytokine levels was assessed by regression analysis. The mean of the ratios of IL-8 to IL-10 was compared in each trimester using anova. RESULTS: The median (range) IL-8 concentrations in cervical secretions were in pg/mL: 1562 (1210-4100), 2460 (1047-4688), 3660 (1451-4748) (P < 0.0021); the median (range) IL-10 concentrations in cervical secretions were in pg/mL: 38.3 (6.8-227.9), 10.9 (0-263.3), 9.5 (0-35.6); the mean IL10/IL-8 x 100 (+/- standard deviation) concentrations were: 3.33 +/- 0.65, 1.47 +/- 0.41, 0.38 +/- 0.52 (P = 0.0035) during the first, second and third trimesters, respectively. CONCLUSION: The patterns of cervical IL-8 concentration is inversely related to gestational age, and the ratio of IL-10/IL-8 decreases with advancing gestation.     

Serum IL-6 and IL-8 Correlate with Prognostic Factors in Ovarian Cancer

The aim of the study was to correlate serum levels of IL-2, IL-5, IL-6, IL-8, IL-10, and TNF-α with clinical, laboratory, and pathological prognostic factors in patients with primary ovarian malignancy. Patients treated at the Pelvic Mass Ambulatory of the Discipline of Gynecology and Obstetrics/Oncology Research Institute (IPON) of the UFTM with confirmed diagnosis of malignant ovarian neoplasia (n = 26) were evaluated. Serum collection was performed preoperatively for the determination of tumor markers. The cytokines IL-2, IL-5, IL-6, IL-8, IL-10, and TNF-α were assayed by enzyme-linked immunosorbent assay (ELISA). The prognostic factors were compared using the Mann-Whitney test, with significance level lower than 0.05. When evaluating IL6, it was observed that higher serum levels were associated with overall survival less than 60 months (p = 0.0382). In the evaluation of IL8, higher serum levels were associated with neutrophil-to-lymphocyte ratio (NLR) ≥ 4 and platelet-to-lymphocyte ratio (PLR) ≥ 200 (p = 0.0198 and p = 0.0072, respectively), altered values of serum CA125 (p = 0.0457), and stage IIIC (p = 0.0486). Therefore, increased levels of IL-6 and IL-8 are associated with factors of worse prognosis in ovarian cancer. Additional studies with a larger sample of patients are needed to confirm the role of cytokines as prognostic factors, in the definition of treatment, and in the development of future target therapies.     

对脂多糖攻击小鼠血及肝IL-6和TNF-α含量的影响

目的：研究重组人白细胞介素-10（rhIL-10）对脂多糖（LPS）诱导的血、肝IL-6和TNF-α炎症介质含量变化的影响。 方法： 小鼠腹腔注射LPS建立炎症模型，并同时静脉注射不同剂量的rhIL-10，ELISA法测定12 h、24 h、48 h和72 h肝组织和血清IL-6和TNF-α的含量。 结果： 注射rhIL-10后12 h，肝组织和血清IL-6、TNF-α水平开始下调，24-48 h抑制作用最明显（P＜0.05），72 h后抑制作用减弱，且呈剂量效应关系。 结论： 利用基因工程技术制备的重组人白细胞介素10（rhIL-10）显著下调肝组织和血清IL-6和TNF-α的水平。     

甘氨酸对急性坏死性胰腺炎鼠血和胰腺组织中TNF-α、IL-1β、IL-6、IL-8和IL-10的影响

目的：探讨甘氨酸对急性坏死性胰腺炎鼠血和胰腺组织中TNF-α、IL-1β、IL-6、IL-8和IL-10的影响。 方法： Wistar大鼠150只，随机分为3组：假手术组(SO)、急性坏死性胰腺炎组(ANP)和急性坏死性胰腺炎＋甘氨酸处理组(ANP+Gly), 以牛磺胆酸钠复制急性坏死性胰腺炎大鼠模型, ANP+Gly组大鼠胰管内给予牛磺胆酸钠前10 min静脉给予甘氨酸1 g/kg。观察血和胰腺组织中ET、TNF-α、IL-1β、IL-6、IL-8和IL-10含量变化，同时观察各组大鼠胰腺组织的病理变化、24 h病死率及平均生存时间。 结果: 在0 h、2 h、4 h、8 h和12 h的血和胰腺组织中ET、TNF-α、IL-1β、IL-6、IL-8 和 IL-10水平, ANP组和ANP+Gly组均显著高于SO组(除0 h组及ANP组的IL-10 12 h外) (P<0.01/P<0.05), 且在ANP和ANP+Gly组ET、TNF-α、IL-1β、IL-6和IL-8随病程进展而升高(P<0.05)，在ANP组 IL-10水平随病程进展降低(P<0.05)，而在ANP+Gly组IL-10水平随病程进展增高(P<0.05)。在相同时段除ET在ANP组和ANP+Gly组无显著差异外(P>0.05), 除0 h组外, TNF-α、IL-1β、IL-6和IL-8水平在ANP组显著高于ANP+Gly组(P<0.01)，而 IL-10水平(除0 h和2 h组无差异外)ANP+Gly组显著高于ANP组(P<0.05/0.01)。虽然ANP组和ANP+Gly组大鼠胰腺组织的病理变化无显著差异，但ANP+Gly组大鼠24 h病死率显著低于ANP组(P<0.05)，平均生存时间显著延长于ANP组(P<0.05)。 结论： ET、TNF-α、IL-1β、IL-6 、IL-8 和 IL－10参与了大鼠ANP的病理过程，甘氨酸可减少促炎细胞因子TNF-α、IL-1β、IL-6 和IL-8 的产生，上调抗炎细胞因子IL－10的负调控作用，有助于减轻ANP时炎性细胞因子瀑布样级联反应，降低大鼠24 h病死率及延长平均生存时间。     

Escherichia coli-induced expression of IL-1 alpha, IL-1 beta, IL-6 and IL-8 in normal human renal tubular epithelial cells

The aim of the present study was to investigate whether the IL-1 family cytokines, in addition to IL-6 and IL-8, could be induced in normal human cortical epithelial cells in response to bacterial stimuli. Human renal tissue was obtained from 9 patients undergoing elective tumour nephrectomy. Renal cortical epithelial cells of tubular origin were prepared from the unaffected tissue. The proximal tubular cells were stimulated for 2, 6 and 24 h with a heat-inactivated pyelonephritogenic Escherichia coli strain DS-17. Cultured unstimulated tubular cells served as controls. IL-1 alpha, IL-1 beta, IL-1 receptor antagonist, IL-6, IL-8, IL-10, TNF-alpha, G-CSF and GM-CSF were analysed using immunohistochemistry at the single cell level. The nonstimulated cells were found to express low levels of IL-6 and IL-8 (mean value < 3% of total cells). In contrast, E. coli exposure resulted in significantly increased incidences of IL-6 and IL-8 expressing cells (mean values approximately 18% of total cells) peaking within two hours of stimulation (P < 0.008 and P < 0.02 versus non-stimulated cells, respectively). A gradual decrease was thereafter observed at 6 and 24 h, respectively, although persistently higher compared to controls. A different kinetic response was found for IL-1 alpha, IL-1 beta and IL-1 receptor antagonist-expressing cells, which peaked 24 h after E. coli stimulation (mean values 3--10%) (P < 0.008, P < 0.02, P < 0.02 versus non-stimulated cells, respectively). Low levels of TNF-alpha and GM-CSF were found in 3 of the 9 donated epithelial cells, peaking at 2 h, and IL-10 and G-CSF producing cells in 1 patient each. In conclusion we found that heat-inactivated pyelonephritic E. coli induced a proinflammatory cytokine response in the normal human proximal tubular cells including the IL-1 family, IL-6 and IL-8.