精索静脉曲张大鼠睾丸ABP、核雄激素受体和血清睾酮含量的变化

本实验在人工诱导大鼠精索静脉曲张病理模型的基础上,对睾丸雄激素结合蛋白(ABP)、核雄激素受体(NAR)以及精索静脉血清睾酮水平进行了观察。结果表明,精索静脉曲张组(VG)左、右两侧睾丸细胞核雄激素受体含量均高于假手术对照组(SOG)(P<0.01,P<0.05);睾丸ABP浓度VG与SOG双侧相比无明显差异(P>0.05);精索静脉血清睾酮水平VG左右两侧均显著低于SOG(P<0.001)。研究结果提示,精索静脉曲张睾丸功能障碍可能与血清睾酮浓度低下及睾丸细胞核雄激素受体反应异常有关。     

睾丸活组织直接镜检法诊断无精子症患者的临床意义

目的 探讨睾丸活组织直接镜检法诊断无精子症患者临床意义.方法 对47例无精子症患者进行睾丸活组织检查,同一部位取睾丸组织两份,一份做常规病理组织学检查,另一份将组织撕碎后,立刻在倒置显微镜下检查有无精子及活动精子,然后对两种方法所得结果 进行比较.结果 47例中,病理组织学检查可见精子生成16例(34.0%),组织撕碎后直接镜检发现精子30例(63.8%).后者精子检出率明显高于前者.结论 与常规病理组织学检查方法相比,直接镜检法能更好地反映睾丸内有无精子或活动精子,且简单易行.在进行病理学检查的同时取睾丸组织直接镜检,可为准备进行卵胞浆内精子注射(ICSI)治疗的患者提供更为确切的依据.     

睾丸活检对无精子症病因的探讨

我院泌尿外科于1986年元月～1992年12月先后对20例无精于症男性不育患者进行睾丸活检，年龄最小20岁，最大42岁，婚龄最短仅半年，最长20年．病理检查结果：无生精细胞5例，其中1例伴有明显的间质纤维化．2例血管数目增多及血管纤维化、玻璃样变，生精细胞减少共5例     

睾丸活检在无精子症中的诊断价值

目的：探讨睾丸活检在无精子症中的诊断价值。方法：对83例无精子症患者进行睾丸活体组织检查并根据活检结果结合Johnsen评分法进行评分。结果：睾丸生精功能尚可的（8分以上）35例;有精原细胞的（3分以上）35例;生精功能较差的（2分以下）13例。结论：睾丸活检可直接评价睾丸的生精功能,以及生精障碍的程度,直接指导治疗方案,是男性无精子症患者不可缺少的一种检查方法。     

28例无精子症病人睾丸活检病理特征分析

目的对28例无精症患者病理特征进行总结。方法观察并对比宁夏中卫某乡13例无精症和石嘴山地区15例无精症患者睾丸活检的病理特等。结果13例中卫某乡无精症患者睾丸活检中，6例光镜下有基本相同的病理组织学特征，与石嘴山地区15例无精症患者睾丸活检对比，其病理特点不同（费歇尔精确概率P〈0．05）。结论推断宁夏中卫某乡无精症患者可能有相同的致病因素，该地区男子无精症的可能是一种损害睾丸生精细胞的物质，而对支持细胞及间质细胞无明显损害。     

Glucocorticoid receptor and heat shock protein 90 in peripheral blood mononuclear cells from asthmatics

目的探讨糖皮质激素受体(GR)和热休克蛋白90(HSP90)mRNA在糖皮质激素敏感型(SS)、依赖型(SD) 和抵抗型(SR)哮喘中的表达及其在SR哮喘发病中的作用。 方法采用反转录-聚合酶链(RT-PCR)的方法分别测定正常人(10例)、SS哮喘(10例)、SD哮喘(5例)和 SR哮喘(6例)的外周血单个核细胞(PBMC)中GR mRNA和HSP90 mRNA的表达,并在体外用IL-2、IL-4分 别、联合刺激上述细胞观察其受刺激后GR mRNA和HSP90 mRNA表达的改变情况。 结果 SR哮喘的GR和HSP90 mRNA表达水平最高(分别为0.730±0.171和1.122±0.165),SS哮喘次之 (分别为0.359±0.350和0.885±0.250),SD哮喘最低(分别为0.017±0.008和0.078±0.039)。正常人有 一定表达(分别为0.052±0.013和0.362±0.101)。GR和HSP90 mRNA的表达各组间相比P＜0.05。正 常人、SS、SD和SR哮喘HSP90/GR的比值分别为7.15±1.84、8.39±7.95、5.51±3.30、1.57±0.18,SR哮喘 HSP90/GR比值明显低于前三组(P＜0.05)。IL-2和IL-4单独刺激对SS、SD和SR哮喘的GR、HSP90 mRNA表达无明显影响,二者联合刺激可使SS、SD和SR哮喘GR mRNA表达以及SS、SD哮喘HSP90 mRNA 表达增强,但不能使SR哮喘HSP90 mRNA表达增强。 结论 SR哮喘中HSP90 mRNA表达相对不足造成HSP90/GR比值降低可能是SR哮喘形成的原因之一, IL-2+IL-4对GR和HSP90 mRNA表达的不同调节作用可能是形成SR哮喘HSP90/GR比值降低的原因之一。     

热疗中热休克蛋白90对26S蛋白酶体的调控机制

目的 探讨热疗中热休克蛋白90(HSP90)对26S蛋白酶体的调控机制.方法建立42℃热疗的HepG2肝癌细胞系模型,评价不同热疗持续时间(0、3、6、12、24 h)对细胞内活性氧簇(ROS)和细胞增殖的影响;并对热疗导致Hsp90α和26S蛋白酶体的影响进行分析.结果热疗后细胞内ROS的含量增加(F=28.958,P<0.001),且热疗对细胞的增殖抑制程度随着时间的延长显著增强(F=621.704,P<0.001);热疗导致胞内Hsp90α表达量增加(F=27.403,P=0.035),26S蛋白酶体表达量明显降低(F=164.174,P<0.001),活性也下降(F=133.043,P<0.001);干扰HSP90α后,26S蛋白酶体也减少(F=180.231,P<0.001).结论随着热疗时间延长,细胞内ROS含量增加,热应激和ROS共同导致蛋白持续变性而消耗HSP90,使没有足够的HSP90对26S蛋白酶体组装和稳定导而导致变性蛋白蓄积发生未折叠蛋白反应使细胞死亡.     

热休克蛋白90在门脉高压大鼠内脏高动力循环中的作用

目的研究热休克蛋白90(HSP90)在门脉高压大鼠内脏高动力循环中的作用。方法20只SD大鼠制成门脉高压动物模型,10只大鼠行假手术作为对照。4周后处死动物获取肠系膜动脉。测定其对甲氧胺(MTX)和乙酰胆碱的量效曲线。然后再灌注格尔德霉素(GA),再次测量其量效曲线。去内膜后,再测量肠系膜动脉对硝普钠(SNP)的量效曲线。结果肠系膜动脉对MTX的反应性降低。而GA能增强门脉高压大鼠肠系膜血管对MTX的反应性。GA还能减少肠系膜血管乙酰胆碱依赖的血管扩张,但不能减少SNP依赖的血管扩张。结论本研究说明了HSP90在门脉高压大鼠内脏高动力循环中起重要作用。     

热休克蛋白90在胃癌患者外周血中的表达及其临床意义

本研究通过对比胃癌者和健康志愿者在血清热休克蛋白90(HSP90)含量方面的差异、评价血清HSP90胃癌诊断价值、分析胃癌患者血清HSP90表达与胃癌临床病理特征关系,以探讨HSP90对胃癌患者的诊断价值。结果发现胃癌组血清HSP90显著高于健康志愿者,工作特征曲线下面积0.754,约登指数最大值0.429,灵敏度80.00%、特异性62.86%,而血清HSP90与胃癌患者性别、年龄、肿瘤直径、临床分期、浸润深度、组织分化、淋巴结转移等均无显著相关,由此可见,血清HSP90对于胃癌诊断有一定参考价值,但与肿瘤分期、转移度等无相关。     

人肾癌组织中热休克蛋白70的纯化与鉴定分析

目的探讨人肾癌组织中热休克蛋白70纯化的有效方法，并进行鉴定分析。方法应用两次亲和层析和离子交换层析获得目的蛋白，经电泳和Western-blot定性分析，应用改良的Bradford法定量分析。结果经过二次亲和层析和Mono Q柱的分离后，得到纯度较高的分子量约为70kDa的蛋白质，经免疫印迹分析证实该蛋白质即为HSP70。与其他方法相比HSP70的纯度更高，获得率接近。结论该文所述方法是一种简单有效的纯化肿瘤组织中HSP70的方法。     

热休克蛋白27在大鼠反流性食管炎食管组织的表达

Background  Little attention has been paid to the expression of heat shock protein 27 (HSP27) in patients with reflux esophagitis (RE), and few studies of the importance of HSP27 in esophagitis have been carried out in animal models. This study aimed to explore the expression of HSP27 in the esophageal tissue of rats with RE.

Methods  Eighty female Wistar rats were randomly divided into experimental groups A and B and control groups C and D (n=20 in each group). To establish RE, rats in the two experimental groups received pylorus and forestomach ligations, while rats in the control group received gastrostomy and gastric perforation repair. The rats in groups A and C were sacrificed 7 days after surgery, and the rats in groups B and D were sacrificed 14 days after surgery. In groups A and B, 10 and 8 rats were diagnosed with RE by pathological examination, respectively (they were included in groups A’ and B’, respectively). The histopathological diagnosis of all the lower esophageal tissues in groups C and D was normal and 20 normal specimens were randomly selected for groups C’ and D’ with 10 specimens in each group. Macroscopic and microscopic esophagitis scores were assessed for the specimens in groups A’ and B’. Lower esophageal tissues were collected from groups A’, B’, C’, and D’, and paraffin-embedded slices were made using part of the tissues. The expression of HSP27 in the tissues was detected using the two-step streptavidin-peroxidase immunohistochemical method. Some collected tissues were frozen, and expressions of HSP27 mRNA were detected using fluorescence quantitative polymerase chain reaction (FQ-PCR).

Results  Median macroscopic and microscopic esophagitis scores in groups A’ (n=10) and B’ (n=8) were 1.0 and 1.5, and 2.0 and 2.5, respectively. There were no significant differences in the macroscopic or microscopic esophagitis scores between the two groups (Z=–0.330, P=0.741; Z=–0.142, P=0.887, respectively). Immunohistochemical staining showed that HSP27 was expressed in all layers of the esophageal epithelia in RE and control rats. FQ-PCR showed that HSP27 mRNA levels in the lower esophageal tissue in RE group (groups A’ and B’) were higher than those in control group (groups C’ and D’) (Z=–0.249, P=0.001). HSP27 mRNA expression in the lower esophageal tissue was significantly different in groups B’ and D’ (Z=–3.027, P=0.002). And the levels of HSP27 mRNA expression in severe RE group (microscopic esophagitis score: 3) were higher than in mild RE group (microscopic esophagitis score: 1–2) and control group (Z=–3.396,P=0.001; Z=–3.855, P <0.001).

Conclusions  HSP27 mRNA expression in the lower esophageal tissue of rats with RE is significantly higher than in the normal controls. Although reflux is a persistent stimulating factor, increased expression of HSP27 in the lower esophageal tissue of rats with RE requires aggravated esophageal injury.

     

Study of heat shock protein HSP90α, HSP70, HSP27 mRNA expression in human acute leukemia cells

Summary The expression of three heat shock proteins (HSPs)-HSP90α, HSP70, HSP27 in cells obtained from 22 patients with leukemia, K562 erythroleukemia cell line, and normal blood cells was observed by means of RNA dot blot analysis. The results showed that the expression of the HSP27 gene was enhanced in 4 cases of acute lymphoid leukemia (ALL), 7 cases of acute nonlymphoid leukemia (ANLL) and 2 cases of myelodysplastic syndrome. (MDS) as compared with that of the normal blood Cells, yet there was no significant difference in the HSP27 expression between the ALL and ANLL cells. The expression of HSP70 in all the 5 ALL and ANLL patients was much lower than that of the normal subjects, except 1 case of ALL and 1 case of MDS, in which the expression was obviously enhanced. All the cases including 11 ANLL, 5 ALL and 1 MDS had higher HSP90α expression than the normal subjects. The enhanced expression pf HSP90α in leukemia cells may be associated with the active and indefinite proliferation of leukemia cells. Our results also suggest that the high expression of the HSP27 gene may not be confined to a specific type of acute leukemia.     

子宫内膜热休克蛋白70和90表达与ER表达的关系

目的　探讨不同子宫内膜中热休克蛋白 (HSP) 70和90表达与 ER表达的关系 .方法　用免疫组化 Envision法和图像分析仪检测了 30例正常子宫内膜、30例增生过长子宫内膜和 5 3例子宫内膜癌中 HSP70、HSP90和雌激素受体(ER)的表达 .结果　增生期内膜中 HSP90和 ER的表达(6 2 .1± 6 .0 ) %和 (6 7.80± 4 .2 ) % ,明显强于分泌期内膜(42 .0± 5 .2 ) %和 (40 .2± 7.8) % ,P<0 .0 5 ,增生过长内膜中表达进一步增强 (74 .2± 2 .2 ) %和 (72 .6± 2 .8) % ,P<0 .0 5 ,而内膜癌中表达较增生期和增生过长内膜减弱 (5 1.3±2 .6 ) %和 (48.7± 5 .2 ) % ,P<0 .0 5 ;分泌期内膜中 HSP70的表达 (6 5 .2± 1.0 ) % ,较增生期内膜增强 (44 .1± 3.2 ) % ,P<0 .0 5 ,内膜癌中表达较分泌期内膜更强 (75 .3± 3.8% ) ,P<0 .0 5 .内膜癌中 HSP90表达随病理分级升高而减弱 (6 7.1±5 .3) % ,(49.1± 1.2 ) %和 (2 9.3± 3.1) % ,P<0 .0 5 ,非子宫内膜样癌中 HSP90表达 (5 1.2± 3.2 ) %较内膜样癌减弱(6 7.2± 6 .0 ) % ,P<0 .0 5 ;内膜癌中 HSP70表达随病理分级升高而增强 (48.2± 5 .0 ) % ,(6 2 .7± 4 .3) %和 (75 .2±2 .8) % ,P<0 .0 5 ,非子宫内膜样癌中 HSP70表达 (74 .1±4 .2 ) %较子宫内膜样癌增强 (6 0 .6±