FT3、FT4、TSH与T3、T4联检评价甲状腺功能及意义

目的探讨甲状腺功能亢进、甲状腺功能减退患者血清中FT3、FT4、TSH与T3、T4水平的变化,以及在评价甲状腺功能中各项指标的诊断符合率及其临床意义。方法应用化学发光免疫分析法测定60例甲状腺功能亢进患者和10例甲状腺功能减退患者血清中的FT3、FT4、TSH与T3、T4的含量,并与50例健康人作对照进行比较。结果疾病组与健康对照组T3、T4、FT3、FT4、TSH血清测定结果比较,差异有统计学意义（P〈0．05）;疾病组与健康对照组T3、T4、FT3、FT4、TSH的诊断符合率比较,差异无统计学意义（P〉0．05）。结论FT3、FT4、TSH与T3、T4联检评价甲状腺功能,对于甲状腺疾病的早期诊断、鉴别诊断及预后判断有着非常重要的临床意义;对临床医生合理用药,提高甲状腺疾病的治愈率和好转率有着科学的指导意义。     

磁分离酶标免疫分析法与放射免疫分析法测定甲状腺五项的比较

目的：比较磁分离酶标免疫分析法（IEMA）与放射免疫分析法（RIA）测定血清甲状腺五项[三碘甲状腺原氨酸（T3）、甲状腺素（T4）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）、促甲状腺素（TSH）]的结果。方法：分别用IEMA法和RIA法检测健康人112名、甲状腺功能亢进（甲亢）病人82例、甲状腺功能减退（甲减）病人37例、甲状腺瘤病人13例及其他疾病病人17例的血清T3、T4、FT3、FT4、TSH,并作对照。结果：IEMA法测定的健康人TSH水平,略显偏态分布;甲亢组：TSH水平显著降低,T3、T4、FT3、FT4显著增高;甲状腺瘤组和其他疾病组甲状腺五项的水平与正常组差异无统计学意义。结论：与RIA法比较,两法结果符合率达92％以上,呈高度相关。方法的可靠性用变异系数（CV）表示;批内CV为1．3％至2．3％,批间2．0％至3．7％。试用结果提示IEMA试剂灵敏、特异、有效、它与RIA法的临床使用价值相当。     

不孕女性甲状腺功能的检测分析

目的：探讨甲状腺功能检测在不孕女性诊治过程中的临床实用价值。方法采用电化学发光免疫技术,分别检测160例不孕女性（不孕组）和160例健康体检女性（健康对照组）的血清促甲状腺激素（TSH）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、抗甲状腺过氧化物酶抗体（TPOAb）和抗甲状腺球蛋白抗体（TGAb）水平,并进行比较分析。结果不孕组女性TSH、TPOAb、TGAb水平明显高于对照组,而T3水平明显低于对照组,差异有统计学意义（P＜0．05）;不孕组TSH、T3、TPOAb和TGAb的阳性率分别为20．63％、16．25％、17．50％和15．63％,对照组阳性率分别为5．63％、2．50％、5．00％和3．75％,不孕组阳性率明显高于对照组,差异有统计学意义（P＜0．05）。结论甲状腺功能异常与不孕症有密切的关系。     

磁性分离免疫法检测T3、T4、TSH的评价

目的用Serozyme-IEMA分析仪检测血清T3、T4、TSH,进行临床和实验室考核。方法将本分析仪与放免法(RIA)进行对比研究。结果健康人(N=482)血清T3、T4、TSH水平(x±s)分别为1.2±0.4ng/ml、92.0±18.1μg/dl、1.7±1.6μIU/ml;初诊甲亢组T3、T4水平明显升高TSH水平明显下降,随着治疗,T3、T4降低TSH升高;甲减组T4水平降低TSH水平明显升高;甲状腺瘤、甲状腺肿、甲状腺结节等各疾病组与正常对照组无明显差异。仪器精密度和准确度均良好:T3、T4、TSH的批内和批间变异系数(CV%)分别为2.5～5.5%、1.6～4.4%、1.1～2.6%和5.5～9.5%、4.6～7.4%、3.1～7.6%;回收率分别为91～105%、91～107%、91～109%。T3、T4、TSH联合检测对甲亢和甲减诊断的敏感性和特异性分别为93.6%、94.6%和98.1%、97.8%。结论本分析仪对甲状腺功能的检测敏感、特异,完全可替代RIA,作为基层实验室检测甲状腺功能的良好仪器。     

彩色多普勒超声血流参数联合T3、T4、TSH早期诊断甲状腺功能亢进症的临床价值

目的:探讨彩色多普勒超声血流参数联合三碘甲状腺原氨酸(triiodothyronine,T3)、甲状腺激素(thyroxine,T4)、促甲状腺激素(thyroid stimulating hormone,TSH)早期诊断甲状腺功能亢进症的临床价值。方法:选取2021年1月—2023年1月寿光市中医医院收治的甲状腺功能亢进症患者80例,同时选取我院同期健康体检者50例作为对照组。均行彩色多普勒超声扫描及血清T3、T4、TSH水平检测,比较两组彩色多普勒超声血流参数最大血流量(maxi-mum flow value,Vmax)、平均血流量(mean-mum flow value,Vmean)、阻力指数(resistance index,RI)及血清T3、T4、TSH水平,并采用受试者工作特征(ROC)曲线分析上述指标单独及联合诊断甲状腺功能亢进症的灵敏度、特异度。结果:甲状腺功能亢进症组彩色多普勒超声血流参数Vmax、Vmean水平均高于对照组,RI水平低于对照组,差异有统计学意义(P<0.05)。甲状腺功能亢进症组血清T3、T4水平高于对照组,TSH水平低于对照组,差异有统计学意...     

磁性分离免疫法检测T3、T4、TSH的评价

目的用Serozyme-IEMA分析仪检测血清T3、T4、TSH,进行临床和实验室考核.方法将本分析仪与放免法(RIA)进行对比研究.结果健康人(N=482)血清T3、T4、TSH水平(x±s)分别为1.2±0.4ng/ml、92.0±18.1μg/dl、1.7±1.6μIU/ml;初诊甲亢组T3、T4水平明显升高TSH水平明显下降,随着治疗,T3、T4降低TSH升高;甲减组T4水平降低TSH水平明显升高;甲状腺瘤、甲状腺肿、甲状腺结节等各疾病组与正常对照组无明显差异.仪器精密度和准确度均良好:T3、T4、TSH的批内和批间变异系数(CV%)分别为2.5～5.5%、1.6～4.4%、1.1～2.6%和5.5～9.5%、4.6～7.4%、3.1～7.6%;回收率分别为91～105%、91～107%、91～109%.T3、T4、TSH联合检测对甲亢和甲减诊断的敏感性和特异性分别为93.6%、94.6%和98.1%、97.8%.结论本分析仪对甲状腺功能的检测敏感、特异,完全可替代RIA,作为基层实验室检测甲状腺功能的良好仪器.     

血清Sema 5A、RVE1水平与桥本甲状腺炎患者Th17相关因子、甲状腺功能及相关抗体的相关性研究

目的 探讨血清神经轴突导向分子5A(Sema 5A)、溶解素E1(RVE1)水平与桥本甲状腺炎(HT)患者辅助性T细胞(Th)17相关因子、甲状腺功能以及甲状腺特异性自身抗体的相关性。方法 选择2019年2月至2021年8月该院收治的109例HT患者(HT组),分为甲状腺功能正常组(39例)、亚临床甲减组(47例)、临床甲减组(23例),另选择58例甲状腺功能正常的体检健康者为对照组。检测各组血清Sema 5A、RVE1、甲状腺激素[促甲状腺激素(TSH)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)]、Th17相关因子[白细胞介素(IL)-17)、IL-23]以及甲状腺特异性自身抗体[抗甲状腺球蛋白抗体(TgAb)和甲状腺过氧化物酶抗体(TPOAb)]水平。分析HT患者血清Sema 5A、RVE1水平与TSH、FT3、FT4、IL-17、IL-23、TgAb、TPOAb等的相关性。结果 HT组血清Sema 5A、IL-23、IL-17、TSH、TgAb、TPOAb水平,外周血Th17占比高于对照组(P<0.05),血清RVE1、FT3、FT4水平低于对照组(P<...     

血清TSH，TH水平在妊娠早期的变化分析

目的：通过对妊娠早期妇女与非妊娠育龄期健康妇女之间血清中促甲状腺激素（thyroid-stimulating hormone, TSH）、甲状腺激素（thyroid hormones,TH）[三碘甲状腺原氨酸（triiodothyronine,T3）、甲状腺素（thyroxine,T4）、游离三碘甲状腺原氨酸（free triiodothyronine,FT3）、游离甲状腺素（free thyroxine,FT4）]水平进行比较分析,为临床诊治妊娠期可能出现的相关疾病提供理论依据。方法收集2013年11月～2014年3月就诊于延安大学附属医院门诊的怀孕4～13周的健康妇女171例作为观察组。另外收集同期来延安大学附属医院体检的非妊娠育龄期健康妇女149例作为对照组。对象的入选标准为：无甲状腺疾病的既往史,无地方性甲状腺肿瘤区生活史,经病史及相关检查排除甲状腺功能异常性疾病。血清TSH,T3,T4,FT3,FT4检测方法均为直接化学发光法。以非妊娠育龄期健康妇女的血清 TSH,TH 水平为参考,观察妊娠早期妇女血清TSH,TH水平的变化。结果妊娠早期妇女血清 TSH[1.97（1.17～2.65）mU/L],T3[1.94（1.58～2.37）nmol/L],T4[131.00（111.60～147.80）nmol/L]水平与非妊娠育龄期健康妇女 TSH[2.42（1.73～3.58）mU/L], T3[1.55（1.37～1.86）nmol/L],T4[109.30（94.95～122.90）nmol/L]水平比较,差异有统计学意义（P＜0.01）;血清 FT3[4.73（4.44～4.94）pmol/L],FT4[14.88（13.83～16.33）pmol/L]水平与非妊娠育龄期健康妇女 FT3[4.70（4.37～5.01） pmol/L],FT4[15.06（13.54～17.35）pmol/L]水平比较,差异无统计学意义（P＞0.05）。结论妇女在妊娠早期血清TSH,TH水平有不同程度的变化。孕妇在产检时有必要进行甲状腺功能的检测,尽力杜绝因妊娠期妇女甲状腺功能异常而影响母体和后代的身心健康,达到优生优育的目的。     

血清TT3、FT3、TT4、FT4以及TSH检测意义

目的探讨TT3、FT3、TT4、FT4以及TSH在甲状腺疾病患者中检测的价值;总结分析各指标变化情况。方法收集分析本院200例甲状腺疾病患者,并选取健康者50例作为对照组。用化学发光分析法检测各组甲状腺功能,并对各组各指标的检测值进行比较。结果甲亢组T3、T4、FT3、FT4均高于健康对照组,甲减组T3、T4、FT3、FT4均低于健康对照组。TSH含量甲亢组低于健康对照组,甲减组明显高于健康对照组。与健康对照组比较其差异均有统计学意义。甲亢组FT3诊断符合率为96%,TSH为96%,T3为92%,FT4为90%,T4为88%。甲减组TSH诊断符合率为100%,FT4为93%,T4为90%,FT3为77%,T3为70%。结论 FT3、T3、TSH在甲亢诊断中有临床意义;FT4、T4、TSH在甲减诊断中有临床意义。     

妊娠早期和中期甲状腺功能测定的意义

目的：探讨妊娠早期和中期甲状腺功能测定的意义。方法采用电化学发光免疫分析法对孕早期（T1组）、孕中期（T2组）和未孕妇女（对照组）进行促甲状腺素（TSH）、游离三碘甲状腺原氨酸（FT3）及游离甲状腺素（FT4）水平检测,并对结果进行分析。结果T1组FT3、FT4、TSH水平与对照组差异均有统计学意义（P＜0．05）。孕妇的甲状腺疾病总患病率为28．85％,显著高于对照组（15．00％）,差异有统计学意义（P＜0．05）。甲状腺功能异常的孕妇以甲状腺功能减低和亚临床甲状腺功能减低为主。结论妊娠早期和中期进行甲状腺功能检测有利于优生优育。     

MR colonography: 1.5T versus 3T

MR colonography is a powerful noninvasive method to image colorectal masses and inflammatory bowel disease. This article describes current techniques of MR colonography and compares its implementation at 1.5T and 3T.     

The nucleic acids of T2, T4, and T6 bacteriophages

The deoxyribonucleic acids of the wild type strains of the T₂, T₄, and T₆ bacteriophages have been shown to contain glucose as an integral part of the molecule; the amount of hexose present in each nucleic acid differs. A study of the acid degradation products of the three nucleic acids has revealed that in each instance glucose is linked to the apurinic acid component. In the case of the T₆ nucleic acid it was found that two molecules of glucose are linked to hydroxymethylcytidylic acid. The other mononucleotides contained no glucose. From the results which have been presented here, and from data presented by others, it can be concluded that the three viral nucleic acids differ in that they contain different proportions of free and glucose-substituted hydroxymethylcytidylic acids.     

MR cholangiopancreatography: 1.5T versus 3T

Soon after its introduction in 1991, MR cholangiopancreatography has become an established diagnostic tool for the evaluation of the pancreaticobiliary ductal system at a field strength of 1.5T. It remains unclear whether MR cholangiopancreatography performed at 3T will benefit from the higher magnetic field strength or whether a field strength of 1.5T should continue to be considered the gold standard for MR cholangiopancreatography. This article reviews the current literature on the benefits and drawbacks of MR cholangiopancreatography at 3T compared with a standard field strength of 1.5T. Field strength-related artifacts that affect MR cholangiopancreatography at 3T also are discussed.     

Reprogramming of virus-specific T cells into leukemia-reactive T cells using T cell receptor gene transfer

T cells directed against minor histocompatibility antigens (mHags) might be responsible for eradication of hematological malignancies after allogeneic stem cell transplantation. We investigated whether transfer of T cell receptors (TCRs) directed against mHags, exclusively expressed on hematopoietic cells, could redirect virus-specific T cells toward antileukemic reactivity, without the loss of their original specificity. Generation of T cells with dual specificity may lead to survival of these TCR-transferred T cells for prolonged periods of time in vivo due to transactivation of the endogenous TCR of the tumor-reactive T cells by the latent presence of viral antigens. Furthermore, TCR transfer into restricted T cell populations, which are nonself reactive, will minimize the risk of autoimmunity. We demonstrate that cytomegalovirus (CMV)-specific T cells can be efficiently reprogrammed into leukemia-reactive T cells by transfer of TCRs directed against the mHag HA-2. HA-2-TCR-transferred CMV-specific T cells derived from human histocompatibility leukocyte antigen (HLA)-A2+ or HLA-A2- individuals exerted potent antileukemic as well as CMV reactivity, without signs of anti-HLA-A2 alloreactivity. The dual specificity of these mHag-specific, TCR-redirected virus-specific T cells opens new possibilities for the treatment of hematological malignancies of HLA-A2+ HA-2-expressing patients transplanted with HLA-A2-matched or -mismatched donors.     

3.0T MR T1及T2 mapping评价肩关节软骨退变

目的 观察3.0T MR T1及T2 mapping在肩关节软骨退变中的应用价值。方法 对30例肩关节疼痛患者（患者组,34肩）和30名正常人（正常组）采用3.0T MR仪行肩关节常规、T1及T2 mapping序列扫描,按国际软骨修复协会（ICRS）分级标准对肩关节软骨分级,分别测量其外、中、内带T1及T2 mapping值;比较组间各指标差异,分析T1及T2 mapping值与ICRS分级及年龄的相关性。结果 患者组34肩中,24肩（24/34,70.59%）关节软骨分级ICRSⅠ~Ⅱ级（轻度退变亚组）,10肩（10/34,29.41%）Ⅲ~Ⅳ级（重度退变亚组）;正常组30肩关节软骨分级均为ICRS 0级。患者组肩关节软骨T1及T2 mapping值均高于正常组（P均<0.05）;其中重度退变亚组T1 mapping值高于轻度退变亚组及正常组（P均<0.05）,轻度退变亚组与正常组间差异无统计学意义（P>0.05）。肩关节软骨外、中、内带T1 mapping值与ICRS分级均呈低度正相关（r=0.282、0.449、0.343,P均<0.05）;T2 mapping值与ICRS分级均呈明显正相关（r=0.635、0.739、0.746,P均<0.05）。随软骨退变程度加重,T1及T2 mapping值呈上升趋势（P均<0.05）。肩关节软骨ICRS分级与年龄呈明显正相关（r=0.678,P<0.05）;T1及T2 mapping值与年龄均呈低度正相关（r=0.393、0.438,P均<0.05）。结论 T1及T2 mapping值可量化肩关节软骨退变,并与年龄相关;T1及T2 mapping序列可作为常规MR评估肩关节软骨退变的重要补充。     

Clonal heterogeneity in the requirement for T3, T4, and T8 molecules in human cytolytic T lymphocyte function

In an attempt to define the requirement of T8, T4, and T3 surface molecules in functional interactions occurring between human cytolytic T lymphocytes (CTL) and specific target cells, we have analyzed a large number of CTL clones derived from primary mixed lymphocyte culture (MLC) T cell populations for their susceptibility to inhibition by monoclonal antibodies (mAb) directed against these surface antigens. In most experiments, MLC T cells were stained with B9.4 (anti-T8) or OKT4 (anti-T4) mAb, separated into positive and negative cells using a fluorescence-activated cell sorter (FACS) and cloned under limiting conditions. While the lytic activity of the majority of T8+ CTL clones was inhibited by B9.4 mAb, approximately 15% of these clones were unaffected even in the presence of excess antibody. Flow cytofluorometric analysis of T8 antigen in individual clones did not show any correlation between the amount of T8 antigen expressed, the magnitude of cytolytic activity and the susceptibility (or lack thereof) to inhibition by B9.4 mAb. Of the 16 T4+ CTL clones analyzed, 7 were resistant to inhibition by OKT4 mAb even at doses 10-fold higher than that sufficient for complete inhibition of susceptible clones. Again, no correlation was found between the amount of T4 antigen expressed and the susceptibility to inhibition by the corresponding antibody. The same sets of T8+ and T4+ CTL clones were also analyzed for their susceptibility to inhibition by OKT3 mAb. Although all of the clones expressed the T3 surface antigen, only 15/23 T8+ clones and 9/14 T4+ clones were inhibited by anti-T3 mAb. To further document this clonal heterogeneity, we selected two T3+ T4- T8+ CTL clones that had no concomitant NK-like activity. One clone was resistant to inhibition by OKT3 mAb, whereas the other was highly susceptible. Incubation with OKT3 mAb resulted in modulation of the T3 molecules in both clones. Following modulation, however, the cytolytic activity of the resistant clones was unaffected, whereas the lytic activity of the susceptible clone was abrogated. These results thus indicate extensive clonal heterogeneity in the requirement for T3, T4, and T8 molecules in CTL function. Moreover, it appears that T3 molecules are not always physically and functionally linked to CTL receptor structures.     

Regulatory T cells--the renaissance of the suppressor T cells

Immune reactions are stringently regulated and balanced by complex interactions of stimulating and suppressing mechanisms. Dysfunctions of this sophisticated immune regulatory network can lead to a variety of diseases such as autoimmunity, allergy, cancer, and pregnancy disorders. The rediscovery of suppressor T cells a decade ago--now designated as T regulatory cells--set off a huge avalanche of research activities leading to a multitude of preclinical and clinical studies. Herein, we give a comprehensive review about this research on T regulatory cells and the relevance of this suppressive T cell population for the development of innovative immune therapeutic strategies.     

Liver MR imaging: 1.5T versus 3T

This article focuses on technical challenges in transferring 1.5T liver protocols to 3T systems and the overall comparison of MR sequences, highlighting the advantages and disadvantages of imaging at the higher field strength. An important benefit is the capacity of acquiring high-quality, thin-section postgadolinium T1-weighted three-dimensional gradientecho sequences, most clinically relevant for the detection and characterization of small hypervascular malignant diseases. Further research and development is necessary to overcome disadvantages, such as with in- and out-of phase T1-weighted gradient-echo sequences, and to minimize artifacts that appear at 3T.