Modulating effect of Leptadenia reticulata (Retz) Wight & arn against chromate (VI)-induced immunosuppression and oxidative stress on mouse splenic lymphocytes and bone marrow derived macrophages

Ethnopharmacological relevance

Leptadenia reticulata (Retz) Wight & arn is mentioned in the ancient ayurvedic literature as an immune booster and rejuvenator.

Aims of the study

To investigate, the effects of different forms of the extract of Leptadenia reticulata [Aqueous extract (JAE), Padavashesha kashaya (JPK) and Tarpana kashaya (JTK)] to alleviate the experimental immunosuppression induced by the immunotoxicant chromate (VI) in vitro.

Materials and methods

Standard cell proliferation and cytotoxicity assays like MTT assay, trypan blue dye exclusion test, neutral red dye uptake test, NBT reduction test, determination of percentage cell survival and estimation of markers of oxidative stress were performed in the study. The study was conducted on primary cultures of mouse splenic lymphocytes and bone marrow derived macrophages.

Results and Conclusions

Treatment with all the three forms of the extract used in the study offered protection against chromate (VI)-induced immunosuppression and the overall protective effect was found to be superior in the case of the aqueous extract of Leptadenia reticulata (JAE). These results confirm that Leptadenia reticulata acts as a modulator and alleviates the immunosuppressive conditions induced by chromate (VI).     

Ethnomedicinal application of plants in the western plain region of North Jeolla Province in Korea

Ethnopharmacological relevance

This study aims to record and conserve oral traditional knowledge of medicinal plants from the indigenous people living in the western plain region of North Jeolla Province, Korea.

Materials and methods

Our research team interviewed 46 key informants (20 men and 26 women) who have lived over 50 years in the area. The average age of the informants was 74 (with a minimum age of 56 to maximum 84). Data was collected through the participatory rural appraisal method involving interviews, informal meetings, open and group discussions, and overt observation using semi-structured questionnaires.

Results

Ethnobotanical inventory possessed by the residents included 183 species of plants distributed in 156 genera belonging to 68 families and were used in 626 use-reports. Among them, the medicinal plants were comprised as 42 families, 75 genera, and 83 species in usage in 195 ways. A total of 71 ailments were treated with 222 kinds of remedies using medicinal plants. Parts of plant that were used as treatments total up to 17 and the methods of preparation for medicinal materials add up to 28. Informant consensus factor (ICF) showed that the muscular-skeletal disorders had the highest agreement measure (1.0) followed by cuts and wounds (0.87) and respiratory system disorders (0.77). Overall, 29 plant species showed fidelity level of 100%.

Conclusions

This study is noteworthy in that it records rapidly disappearing traditional knowledge of medicinal plants in a time period of pressing environmental changes with regard to the newly built sea wall, eventually leading more or less to the immigration of the seaside inhabitants and the familiarization of the modern medical systems to the farmland inhabitants.     

Analysis of ethnoveterinary treatments for cattle (Bos indicus) diseases referred in Sanrimgyeongje including twelve volumes of literature from the 7th to the 18th century

Aim of the study

This study aims at a comprehensive analysis of ethnoveterinary treatment in the cattle-raising section of Sanrimgyeongje which covers 12 different volumes of literature including 4 Korean and 8 Chinese literatures from the 7th to the 18th century, with a special attention to the treatments for cattle diseases.

Materials and methods

The above mentioned literature was analyzed through several steps: translation of the Chinese text into Korean, identification of diseases, verification of medicinal materials and confirmation of scientific names.

Results

As recorded in the thirteen references, this study shows that there are 143 medicinal materials which have been used as therapies for cattle diseases. Of these, 55 plant species belonging to 52 genera in 29 families had 100 modes of usages, while one species of fungus was used in one way. Likewise, 11 species of animals belonging to 11 genera in 10 families were utilized in 18 different methods. Lastly, 9 kinds of inorganic matters were used in 11 ways with another 4 kinds being useful in 14 different ways. Consequently, each of rinderpests, murrains, and hooves has been treated, respectively with 14, 10 and 3 types of medicinal decoctions made from 35, 18 and 15 kinds of medicinal materials.

Conclusion

This diversified usage of various medicinal materials is incomparable to the modern ethnoveterinary investigation which tends to focus strictly in plants. If additional studies were to be conducted on these treatments and all the medicinal materials used within them, various new treatments and medicines can be developed to supplement the pharmacopoeia of contemporary veterinary medicine.     

Natural pharmacopoeia used in traditional Toba medicine for the treatment of parasitosis and skin disorders (Central Chaco, Argentina)

Aim of the study

To assess the knowledge and use of natural pharmacopoeia for the treatment of parasitosis and skin disorders, as well as for the control of their etiological agents or vectors, of a Toba community in Central Chaco, Argentina.

Materials and methods

Information was obtained by open, extensive and recurrent interviews and semi-structured surveys. Plant and animal pharmacopoeia was documented by collecting material in field assays carried out in the company of informers. The list of applications with the greatest consensus of uses, the list of species with most medicinal applications and the list of species with the highest reputation (according to the level of fidelity) for the aforementioned disorders were obtained using quantitative methods.

Results

A total of 178 medicinal uses were documented corresponding to 87 species (72 plant and 15 animal species) belonging to 51 different families (39 plant and 12 animal families). The most represented families according to the number of species were Solanaceae (7 species), Asteraceae (6 species) and Fabaceae (5 species) for plants, and Bovidae (3 species) for animals.

Conclusions

Although the list of medicinal species includes some symbolical applications, others are supported by phytochemical information. In other cases the applications coincide with other pharmacopoeias of the Gran Chaco region indicating the presence of an active exchange of knowledge through interethnic contacts.     

Medicinal plant use in Vanuatu: a comparative ethnobotanical study of three islands

Ethnopharmacological relevance

Our study shows that large parts of Vanuatu's medicinal flora remain unexplored and that a high variability of medicinal plant knowledge between islands exists.

Aim of the study

The following questions are comparatively analyzed for three islands of Vanuatu: who are the medicinal plant specialists and how important is their knowledge today? Which plants are used to treat common diseases?

Materials and methods

On Loh, Ambrym and Aneityum plant related information was collected using semi-structured interviews, transect walks and participant observation. A total of 29 medicinal plant specialists were interviewed.

Results

Medicinal plant specialists are either peasants or people with a high rank in the local social system such as members of the chief's family or priests. Their knowledge may be very broad (Loh, Aneityum) or specialized on specific diseases (Ambrym). Medicinal plant knowledge is transmitted family and gender specific (Loh) or gender and family independent (Ambrym and Aneityum). Overall, 133 medicinal plant species were documented of which 117 are new to Vanuatu's ethnopharmacopoeia. Mainly members of the Euphorbiaceae and Fabaceae, followed by Asteraceae, Convolvulaceae, Moraceae and Zingiberaceae are utilized. The majority of documented species are trees (33%), followed by herbs (22%) and shrubs (21%). Leaves accounted for the highest number of use reports (43%). The highest diversity of medicinal plants is found for the most common diseases such as skin, gastrointestinal, respiratory system and urogenital system diseases. Only a small overlap of taxa between the islands was found.

Conclusions

The biocultural diversity of Vanuatu is reflected in the variability of medicinal plant knowledge and differences in the traditional medicinal system between the three islands investigated. Traditional medicine is more vital on remote islands. The better connected the islands are to the main city, the more dominant western medicine becomes and traditional medicine mainly remains to treat illnesses with a magical origin.     

Ethnopharmacological survey of medicinal herbs in Jordan, the Northern Badia region

Aim of the study

This study sought to gather information from aboriginal Bedouins in North Badia region of Jordan about used medicinal herbs besides their folk uses.

Materials and methods

The data were collected from 40 practitioners who utilized medicinal plants and who were regarded as professional. Subsequently, the uses were compared with the reported ones in the literature. The informant consensus factor (Fic) and use value (UV) have been calculated to those herbs and the managed illnesses.

Results

The data of 73 species were collected; the vast majority of them are safe such as Achillea falcata, Tamarix aphylla and Teucrium polium. Treatment of inflammation and pain presented the major targeted use of these herbs. While, the herbs used for delivery and female problems were limited. This might be due to the culture conservations about the talk of feminine issues. Diseases of kidney, gastrointestinal and respiratory systems as well as diabetes depicted the largest Fic values. Artemisia herba-alba possessed the highest UV value among the studied herbs.     

Ethnopharmacological use of babassu (Orbignya phalerata Mart) in communities of babassu nut breakers in Maranhão, Brazil

Aim of the study

Products derived from the palm tree Orbignya phalerata Mart, a species popularly known in Brazil as babassu, are used for the treatment of different diseases such as rheumatism, ulcers and inflammatory processes. The objective of this study was to perform a sociodemographic survey among communities of babassu nut breakers in the region of Médio Mearim, Esperantinópolis, Maranhão, Brazil, and to evaluate the frequency and main forms of therapeutic use of babassu products and subproducts.

Materials and methods

A semiquantitative method was used for the survey, with the application of semistructured interviews consisting of closed and semi-open questions. The data were analyzed using the SPSS 16.0 software for Windows (2007).

Results

The results showed that 82% of female nut breakers had a household income of less than US$ 280.00 and only 2% earned more than US$ 500.00. With respect to race and educational level, 61% of the interviewed women were mulattoes and 33% had a low educational level. The babassu-derived products most frequently used for medicinal purposes were the mesocarp, a residue called “borra”, and oil. The mesocarp flour was used by 90% of nut breakers for the treatment of gastritis and inflammation and by 77% for leukorrhea. Babassu residue was mainly used for the treatment of wounds (60%) and the oil was used for woundhealing (16%) and leukorrhea (8%).

Conclusions

In conclusion, the products derived from babassu are used for the treatment of gastritis, leukorrhea and wounds. In addition, most of the interviewed subjects apply these products topically.     

Identification of medicinal plants used as Tibetan Traditional Medicine Jie-Ji

Aim of the study

Based on the authors’ collection of specimens used as Jie-Ji in local Tibetan areas, China, and taxonomic determination, this paper aims to give a list of medicinal plants as Jie-Ji, formally identify the ones recognized as Jie-Ji Ga-Bao or Jie-Ji Na-Bao and to offer basic data for further studies on these Tibetan herbs.

Materials and methods

Local herbalists were visited in Tibetan areas, China to observe which plants were being used as Jie-Ji. Samples of the indigenous plants were collected at the same time. Also, the medicinal plants as Jie-Ji were taxonomically identified.

Results

A list of medicinal plants including 10 species of Jie-Ji in local Tibetan areas is given, including their morphological pictures used for identification.

Conclusions

The origin of Jie-Ji is from 10 species of the Section Cruciata, Genus Gentiana (Gentianaceae). five species with dark blue flowers are used as Jie-Ji Na-Bao, the other five with white flowers are used as Jie-Ji Ga-Bao. Also, Gentiana macrophylla Pall. with dark blue flowers in the Section Cruciata, Genus Gentiana is not the original plant of Jie-Ji Na-Bao. The species endemic to the province are used as the original plants of Jie-Ji only in local Tibetan area of the province. Finally, the drug use of Jie-Ji in Traditional Tibetan Medicine is reasonable and it is efficacious.     

Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects

Ethnopharmacological relevance

Peppers from Capsicum species (Solanaceae) are native to Central and South America, and are commonly used as food and also for a broad variety of medicinal applications.

Aim of the study

The red pepper Capsicum baccatum var. pendulum is widely consumed in Brazil, but there are few reports in the literature of studies on its chemical composition and biological properties. In this study the antioxidant and anti-inflammatory activities of Capsicum baccatum were evaluated and the total phenolic compounds and flavonoid contents were determined.

Materials and methods

The antioxidant property was assayed by scavenging abilities using DPPH and the anti-inflammatory activity was tested through the carrageenan-induced pleurisy model in mice. The total phenolic compounds and flavonoid contents were determined spectrophotometrically.

Results

The ethanolic and butanol extracts (200 mg/kg, p.o.) presented a significant anti-inflammatory activity toward carrageenan-induced pleurisy model in mice in comparison to dexamethasone (0.5 mg/kg, s.c.). Among the parameters evaluated, the treatment with these samples inhibited leukocyte migration and reduced the formation of exudate. The contents of flavonoids and total phenolic compounds could be correlated with the antioxidant and anti-inflammatory activities observed for Capsicum baccatum.

Conclusions

Our findings suggest that Capsicum baccatum contains potential antioxidant and anti-inflammatory compounds which could be tested as drug candidates against oxidative and inflammation-related pathological processes in medicinal chemistry studies.     

Diuretic activity and acute oral toxicity of Palicourea coriacea (Cham.) K Schum

Aim of the study

Palicourea coriacea (Cham.) K Schum, is an endemic plant used in the Midwestern Region of Brazil, popularly known as “douradinha do campo” and “congonha do campo”. This plant has been used in traditional medicine for several ailments, especially to treat kidney diseases. Since no formal studies on the biological activities and medicinal properties of the ethanolic extract of Palicourea coriacea (PCEE) have been carried out previously, the present study represents the first research into the efficacy of this plant as a diuretic agent employing laboratory rats as test animals.

Materials and methods

For diuretic activity evaluation we assayed three doses of PCEE (20, 40 and 80 mg/kg) and measurement of the urinary volume and electrolytes (Na⁺, K⁺) concentration were taken. The acute oral toxicity of PCEE was investigated according to OECD Guideline 423.

Results

The oral administration of a single dose of PCEE significantly increased the urinary volume in 24 h. Additionally, the treatment with PCEE increased, in a dose-dependent manner, the excretion of both, Na⁺ and K⁺. No sign of toxicity was observed in the animals.

Conclusions

The present study confirmed the ethnopharmacological use of Palicourea coriacea as a diuretic agent in the experimental condition tested here. Additionally, this work supports the importance of the preservation of local knowledge as well as the conservation of Brazilian biodiversity.     

Change in medical plant use in Estonian ethnomedicine: a historical comparison between 1888 and 1994

Aim of the study

The aim of this paper is to compare the changes in the utilization of species from various hemeroby categories (indicating the degree of sensitivity of the plant to human impact) using historical data concerning the years 1888-1994.

Materials and methods

The authors digitised 8808 handwritten reports, reflecting local ethnopharmacological knowledge from 8 selected collections from the Estonian Folklore Archives of the Estonian Literary Museum. They were semi-quantitatively analyzed according to the sensitivity to human impact of 540 taxa that could possibly be related to the plant vernacular names given in the reports.

Results

Although in different periods of time the number of ethnopharmacologically used plants has changed, the proportion of plants utilized from each group has remained relatively same, consisting on average of: 23% anthropophytes, 42% apophytes, 32% hemeradiaphores and 3% hemerophobes. Comparison of the application of the most used plants revealed considerable changes of plant utilization, in which the varied use of the most popular anthropophytes increased and the applied scope of the most popular hemeradiaphores and hemerophobes decreased almost by twofold in one century. Case studies on seven taxa are presented, of them, use of Allium sativum L., Aesculus hippocastanum L. and Mentha xpiperita L. increased, whereas the use of Hordeum L., Orchidaceae, Paris quadrifolia L. and Briza media L. decreased greatly.

Conclusions

This research contributes to the better understanding of the cognitive and human ecological concepts underlying the use of medicinal plants in Estonia. Strong increase in the ethnomedical utilization of plants depending on human influence, and a decrease in the use of taxa that do not prefer human activities indicates that, despite some of the population still have access to natural resources and diverse knowledge of the medical use of plants, the majority relies on a very narrow selection and a rather restricted herbal landscape.     

Analysis and recordings of orally transmitted knowledge about medicinal plants in the southern mountainous region of Korea

Aim of the study

This study aims to analyze and record orally transmitted knowledge of medicinal plants from the indigenous people living in the southern mountainous region of Korea. This article is the first study of its kind.

Materials and methods

Data was collected through the participatory rural appraisal (PRA) method involving interviews, informal meetings, open and group discussions, and overt observations with semi-structured questionnaires.

Results

The investigation reveals that the indigenous people have used 217 species of medicinal plants distributed in 177 genera belonging to 77 families with 691 different uses. The representative families were Asteraceae (13.5%) followed by Fabaceae (8.2%), Araliaceae (6.1%), Rosaceae (5.8%), Cucurbitaceae (4.1%), Poaceae (3.0%), Liliaceae (2.7%), and Apiaceae (2.6%). On the whole, 21 kinds of plant-parts were used and prepared in 26 various ways by the people for medicinal purposes. The informant consensus factor (ICF) values in the ailment categories were veterinary ailments (0.88), pains (0.84), cuts and wounds (0.77), and respiratory system disorders (0.76). In terms of fidelity levels, 41 plant species showed 100% of fidelities.

Conclusion

In recent years, the Korean society has been a heavy loss of traditional practices due to a rapid decrease of the senior population. This pressing circumstance raises the level of urgency for the preservation of indigenous knowledge from extinction. A priority should be given first for collecting and recording indigenous knowledge from the natives inhabiting the national parks and environmental preservation areas.     

Animals used for medicinal and magico-religious purposes in western Granada Province, Andalusia (Spain)

Ethnopharmacological relevance

This paper serves to document and make known the folk medicinal uses of animals and animal products in the western part of the province of Granada (Andalusia, Spain), analyzing not only the species used, the administration methods and the ailments treated, but also the cultural implications of this traditional knowledge and the large percentage of magico-religious or ritual practices.

Materials and methods

The information was gathered through semi-structured open interviews of a total of 42 people, as a part of an in-deep ethnobiological research in 16 different municipalities within the study area. The use-value index was calculated for highlighting the most important species for the population.

Results

In this part of Granada, 26 different animals have been employed in the treatment of 26 conditions from 10 different pathological groups, amounting 150 total reports (7% of the total ones including plant and animal medicinal resources). Although some of the uses were included in several classical and anthropological works, most remain unpublished. Cultural and environmental implications are discussed.

Conclusions

The traditional use of animals in medicine is low known for southern Europe. 26 species constitute a significant number of animals used, considering the socio-economic and cultural context in which the study area is situated, and its small size. Albeit the main part of the information presented in this paper can be more important in an historical or anthropological standpoint, most of the medicinal resources presented have not been deeply studied.     

Brazilian medicinal plants described by 19th century European naturalists and in the Official Pharmacopoeia

Ethnopharmacological relevance

The American flora represents one of the world’s wealthiest sources of material with pharmacological activity due to its biodiversity. Medicinal plants are widely used as home remedies in Brazil but several species used are native of other continents and were introduced here since the colonization, beginning in 1500. The Traditional Medicine Division of the WHO recognizes the importance of plant species used by the Amerindian as medicines, and recommends that their efficacies should be evaluated through pharmacological and toxicological assays.

Aim of the study

To verify which Brazilian medicinal plants, especially those of Amerindian origin, were used in 19th century and have been evaluated by pharmacological studies.

Materials and methods

Data about the use of native plants in traditional medicine were searched in bibliographic material from European naturalists who traveled throughout Minas Gerais in the 19th century.

Results and conclusion

Two hundred and three species were described as useful by these naturalists and thirty-nine of them were also included in the first edition of Brazilian Official Pharmacopoeia (FBRAS) in 1929, showing their use also in conventional medicine. Seventeen species have medicinal properties of Amerindian origin but despite the long tradition of medicinal plant use, only nine have been evaluated by pharmacological studies. That the studies which have been conducted to date have in each case confirmed the traditional uses of the plants examined. We suggest that the remaining species must be regarded as a priority for pharmacological studies, as they have promising phytotherapeutic potential.     

Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.)

Ethnopharmacological context

This review explores the medicinal and therapeutic applications of Sea buckthorn (Hippophae rhamnoides L.) in curtailing different types of acute as well as chronic maladies. The plant is being used in different parts of the world for its nutritional and medicinal properties.

Materials and methods

Sea buckthorn based preparations have been extensively exploited in folklore treatment of slow digestion, stomach malfunctioning, cardiovascular problems, liver injury, tendon and ligament injuries, skin diseases and ulcers. In the recent years, medicinal and pharmacological activities of Sea buckthorn have been well investigated using various in vitro and in vivo models as well as limited clinical trials.

Results

Sea buckthorn has been scientifically analyzed and many of its traditional uses have been established using several biochemical and pharmacological studies. Various pharmacological activities such as cytoprotective, anti-stress, immunomodulatory, hepatoprotective, radioprotective, anti-atherogenic, anti-tumor, anti-microbial and tissue regeneration have been reported.

Conclusion

It is clear that Sea buckthorn is an important plant because of its immense medicinal and therapeutic potential. However, several knowledge gaps identified in this paper would give impetus to new academic and R&;D activities especially for the development of Sea buckthorn based herbal medicine and nutraceuticals.     

In vivo antiulcer activity of the aqueous extract of Bauhinia purpurea leaf

Ethnopharmacological relevance

Bauhinia purpurea (Fabaceae) is a medicinal plant traditionally used to treat various ailments, including ulcers. In order to establish pharmacological properties of the leaf of Bauhinia purpurea, studies were performed on antiulcer activity of the plant's aqueous extract.

Materials and methods

The Bauhinia purpurea aqueous extract (BPAE) was prepared in the doses of 100, 500 and 1000 mg/kg. Antiulcer activity of BPAE was evaluated by absolute ethanol- and indomethacin-induced gastric ulcer, and pyloric ligation models. Acute toxicity was also carried out.

Results

BPAE, at the dose of 5000 mg/kg, did not cause any signs of toxicity to rats when given orally. Oral administration of BPAE exhibited antiulcer activity (p < 0.05) in all models used. However, the dose-dependent activity was observed only in the absolute ethanol-induced gastric ulcer model. Histological studies supported the observed antiulcer activity of BPAE. In pyloric ligation assay, BPAE increased the gastric wall mucus secretion.

Conclusions

The BPAE exhibits antiulcer activity, which could be due to the presence of saponins or sugar-free polyphenols, and, thus, confirmed the traditional uses of Bauhinia purpurea in the treatment of ulcers.     

Malaria in the Renaissance: remedies from European herbals from the 16th and 17th century

Background

From antiquity up into the 20th century tertian and quartan malaria which are caused by the parasites Plasmodium vivax and Plasmodium malariae were widespread in Central Europe. Hundreds of different remedies against malaria can be found in herbals from the Renaissance.

Aim of the study

To document and discuss from a modern pharmacological viewpoint the old remedies described in eight 16th and 17th century herbals written in German.

Materials and methods

Eight of the most important herbals of the 16th and17th century including Bock (1577), Fuchs (1543), Matthiolus (1590), Lonicerus (1560), Brunfels (1532), Zwinger (1696), and Tabernaemontanus (1591 and 1678) were searched for terms related to malaria, and documented plants and recipes described for its treatment. Additionally the overlapping of these remedies with those in De Materia Medica by the Greek physician Dioscorides was studied.

Results

Three hundred and fourteen taxa were identified in the herbals for this indication. Recent pharmacological data was found for just 5% of them. The influence of De Materia Medica was shown to be negligible with only 3.5% of the remedies in common.

Conclusions

European Renaissance herbals may be a valuable source of information for the selection of plants for focussed antiplasmodial screening programmes, but have received only little scientific attention. Antimalarial remedies from these herbals must be viewed as independent sources of knowledge separate from Dioscorides’ De Materia Medica.     

Folk medicinal use of fauna in Mapimi, Durango, México

Aim of the study

To document the use of animal species in traditional medicine from the municipality of Mapimi, Durango, México.

Materials and methods

Direct interviews were performed in several short visits with inhabitants from the municipality of Mapimi, Durango. The interviews were analyzed with two quantitative tools. The informant consensus factor that estimates the level of agreement between interviewees over which animals to use for each category and the fidelity level which indicates the percentage of informants claiming the use of a certain animal species for the same medicinal purpose.

Results

A total of 18 animal species, belonging to 17 families and four taxonomic categories, were reported by interviewees as used for medicinal purposes. Thirteen medicinal species were identified not previously reported. The results of the informant consensus factor showed that the dermatological, diabetes and reproductive categories had the greatest agreement. The most important species according to their fidelity level are: Mephitis macroura and Crotalus atrox.

Conclusion

This study demonstrates that many animal species play an important role in healing practices among inhabitants from Mapimi, Durango. Further experiments with medicinal fauna from Mapimi are needed to be carried out to obtain experimental information about their traditional uses, especially with C. atrox.