含T3温血停搏液对心功能及冠脉内皮细胞功能的影响

目的：观察温血停搏液中的加入０．６ｍｇ／ＬＴ３对停搏心肌有量代谢，左心收缩及舒张功能，心肌酶谱及冠状动脉内皮细胞功能的影响，方法；观察禽体离心停搏前后左室收缩压（ＬＶＳＰ），左室收缩压力微分（ＬＶ＋ｄｐ／ｄｔｍａｘ）左室舒张压力微分（ＬＶ－ｄｐ／ｄｔｍａｘ）及心理氧耗量（ＭＶＯ２）测定鼠心复跳后１ｍｉｎ冠状静脉窦流出液中乳酸脱氢酶（ＬＤＨ），肌酸磷酸激酶（ＣＰＫ）含量。观察乙酰胆碱（Ａｃｈ）和硝普     

含T_3冷晶体停搏液对大鼠心功能及冠脉内皮细胞功能的影响

目的：观察冷晶体停搏液中加入生理浓度Ｔ３对大鼠停搏心肌收缩及舒张功能恢复、心肌酶谱、氧耗量及冠脉内皮细胞功能的影响。方法：运用离体鼠心作功模型，实验组（ｎ＝６）含０．６ｍｇ／ＬＴ３，比较停搏９０ｍｉｎ前后左室收缩压力（ＬＶＳＰ）、左室收缩压力微分（ＬＶ＋ｄｐ／ｄｔｍａｘ）、左室舒张压力微分（ＬＶ－ｄｐ／ｄｔｍａｘ）、肌酸磷酸激酶（ＣＰＫ）、乳酸脱氢酶（ＬＤＨ）的变化。同时评价乙酰胆碱（Ａｃｈ）和硝普钠（ＳＮＰ）所致的冠脉血管舒张功能的改变。结果：实验组ＬＶＳＰ恢复率为（９０．１±３．７）％，ＬＶ＋ｄｐ／ｄｔｍａｘ为（７９．２±５４）％，优于对照组；而ＬＶ－ｄｐ／ｄｔｍａｘ、ＬＤＨ、ＣＰＫ、Ｖｍｏ２（即ＭＶＯ２）两组无显著差异。Ａｃｈ所致的冠状血管阻力（ＣＶＲ）降低两组都不明显，ＳＮＰ所致ＣＶＲ下降程度基本一致。结论：含Ｔ３冷晶体停搏液仅能促进大鼠左室收缩功能恢复，而对舒张功能、心肌氧耗量、心肌酶谱影响不大，Ｔ３由于内皮细胞功能降低而不能明显扩张冠状血管。     

不同时间等容血液稀释对犬急性心肌梗塞左室功能的改善作用

在麻醉开胸纯氧呼吸状态下的犬急性心肌梗塞（ＡＭＩ）模型上，观察以右旋糖酐４０在不同时间（ＡＭＩ前１ｈ、２４ｈ和ＡＭＩ后３０ｍｉｎ）行等容血液稀释对犬ＡＭＩ６ｈ内左室功能（±ｄｐ／ｄｔｍａｘ、ＬＶＳＰ）的改善作用。结果表明：ＡＭＩ后３０ｍｉｎ行血液稀释可使＋ｄｐ／ｄｔ－ｍａｘ明显升高（Ｐ＜０．０５）；而ＡＭＩ前１ｈ、２４ｈ行血液稀释不仅使ＡＭＩ后＋ｄｐ／ｄｔ－ｍａｘ明显升高，ＬＶＳＰ与－ｄｐ／ｄｔｍａｘ也明显升高（Ｐ＜０．０５）。提示：①不同时间行等容血液稀释对犬ＡＭＩ后左室收缩功能均有改善作用；②提前稀释血液不仅改善左室收缩功能，也能改善左室舒张功能。     

人参总皂甙对犬冠脉流量和血液动力学的影响

以两种剂量的人参总皂甙（Ｇｉｎ）静脉注射麻醉犬，观察犬的冠脉流量，血压，ＬＶＳＰ，ＬＶｄｐ／ｄｔｍａｘ，ＨＲ，心肌氧消耗及心泵功能指标。结果显示：２０ｍｇ／ｋｇ的Ｇｉｎ能显著降低麻醉犬的血压，ＬＶＳＰ和ＩＶｄｐ／ｄｔｍｘ，减慢心率，明显减少心肌耗氧，但对泵功能指标ＣＩ和ＣＯ无明显影响，ＳＩ及冠脉流量明显增加；１０ｍｇ／ｋｇ的Ｇｉｎ使血压稍增加，但不明显（Ｐ＞０．０５）。     

人参总皂甙对犬冠脉流量和血液动力学的影响

以两种剂量的人参总皂甙（Ｇｉｎ）静脉注射麻醉犬，观察犬的冠脉流量，血压，ＬＶＳＰ，ＬＶｄｐ／ｄｔｍａｘ，ＨＲ，心肌氧消耗及心泵功能指标。结果显示：２０ｍｇ／ｋｇ的Ｇｉｎ能显著降低麻醉犬的血压，ＬＶＳＰ和ＩＶｄｐ／ｄｔｍｘ，减慢心率，明显减少心肌耗氧，但对泵功能指标ＣＩ和ＣＯ无明显影响，Ｓ汲冠脉流量明显增加；１０ｍｇ／ｋｇ的Ｇｉｎ使血压稍增加，但不明显（Ｐ〉０．０５）。     

大鼠烫伤后心肌力学变化的实验研究

目的：探索大鼠烫伤后心肌舒缩功能变化及其规律。方法：复制大鼠ＴＢＳＡ３０％Ⅲ°烫伤模型,５６只随机分成烫伤前及烫伤后 １、３、６、１２、２４、４８及７２ｈ ８组,观察各时段左心室力学参数（ＬＶＳＰ、ＬＶＥＤＰ、±ｄｐ／ｄｔｍａｘ）变化。结果：动物烫伤后１ｈ,－ｄｐ／ｄｔｍａｘ显著下降,ＬＶＥＤＰ明显升高; ＬＶＳＰ、＋ｄｐ／ｄｔｍａｘ伤后３ｈ显著下降。最显著的变化是伤后１２ｈ,７２ｈ仍较明显（Ｐ＜０．０１）。结论：大鼠烫伤后左心室舒缩功能明显下降,且舒张功能障碍的发生早于收缩功能障碍。     

输精管结扎对家兔心功能及心肌NE含量的影响

本实验用日本大耳白兔复制输精管结扎的长期动物模型，分为结扎２５月组（ＶＧ２５），同龄假手术２５月组（ＳＯＧ２５）；结扎６月组（ＶＧ６），同龄假手术６月组（ＳＯＧ６）。记录各组家兔心功能，检测心肌及血清ＮＥ含量，称取心重（ＷＨ）。结果表明，左心室收缩期末压（ＬＶＳＰ），ＶＧ略高于ＳＯＧ；左心室舒张末压（ＬＶＥＤＰ），Ｖ６２５显著的低于ＳＯＧ２５Ｐ＜０．０５），ＶＧ６与５ＯＧ６比较虽无显著差异，也呈低值；±ｄｐ／ｄｔｍａｘ，ＶＧ均明显高于ＳＯＧ（Ｐ＜０．０１）。相关检验表明，＋ｄｐ／ｄｔｍａｘ与血清及心肌ＮＥ含量无相关性；心重与＋ｄｐ／ｄｔｍａｘ呈明显正相关，Ｐ＜０．０５；在２５月组，ＬＶＳＰ与＋ｄｐ／ｄｔｍａｘ呈正相关，Ｐ＜０．０５。提示输精管结扎可提高心肌收缩功能。     

芬太尼对离体大鼠心脏收缩力及心肌细胞钙离子移动的影响

目的　观察芬太尼对离体灌流心脏收缩力及心肌细胞钙离子移动的影响,研究其对心肌收缩力的作用。方法　（１） 以含不同浓度芬太尼的克汉二氏重碳酸盐缓冲液（ＫＨＢ） 灌流大鼠心脏,测定心率（ＨＲ） 、左室收缩压（ＬＶＳＰ） 、左室舒张压（ＬＶＤＰ）、左室收缩压上升最大速率（ ＋ ｄｐ／ｄｔｍａｘ）、左室舒张压下降最大速率（ － ｄｐ／ｄｔｍａｘ） ;（２） 用Ｆｌｕｏ３ＡＭ 钙荧光指示剂染色急性分离的大鼠心肌细胞,在激光共聚焦显微镜下动态观察用药前和用不同浓度芬太尼后,ＫＣｌ 诱发的细胞内钙离子浓度的变化。结果　１ ｎｇ／ｍｌ 和２０ ｎｇ／ｍｌ 的芬太尼对心肌收缩力和心肌细胞钙离子移动的影响较小,心肌收缩力的各项指标和钙荧光强度与对照组相比,差异无显著性（ Ｐ＞ ０．０５） 。而２００ ｎｇ／ｍｌ 芬太尼使心率减慢及＋ ｄｐ／ｄｔｍａｘ 、－ ｄｐ／ｄｔｍａｘ下降,且差异有显著性（ Ｐ＜０ ．０５） 。结论　芬太尼对心肌细胞钙离子的跨膜内流影响较小,而使心肌收缩力保持稳定。超临床使用浓度的芬太尼仍有抑制心肌的趋势。     

动脉收缩压上升速率评价心肌收缩能力

用动脉收缩压最大上升速率（ｄｐ／ｄｔｍａｘ）和平均上升速率（ｄｐ／ｄｔｍｅａｎ）评价心肌收缩能力，结果显示：硬膜外麻醉下，腹腔探查时，ＭＡＰ，ＨＲ，ＣＯ，ＲＴｍ，ｄｐ／ｄｔｍａｘ和ｄｐ／ｄｔｍｅａｎ均明显下降（Ｐ〈０．０５），而加中小剂量芬太尼复合麻醉上，术前ｄｐ／ｄｔｍａｘ和ｄｐ／ｄｔｍｅａｎ明显下降（Ｐ〈０．０５）ＭＡＰ，ＳＶ，ＣＯ，ＲＴｍ无明显变化（Ｐ〉０．０５）。表明麻醉药对心肌收缩能力有     

培哚普利对心衰大鼠心肌肌浆网Ca^2＋泵活性和Ca^2＋释放通道密度的影响

目的 探讨血管紧张素转换酶抑制剂（ＡＣＥＩ）培哚普利干预慢性心衰对心肌肌浆网（ＳＲ）Ｃａ＾２＋泵活性和Ｃａ＾２＋释放通道（ＲｙＲ２）密度的影响及意义。方法 通过结扎大鼠左冠脉建立慢性心衰模型,以培哚普利进行干预,对照观察血流动力学、左室心肌ＳＲ Ｃａ＾２＋泵活性、〔＾３Ｈ〕－ｒｙａｎｏｄｉｎｅ与ＲｙＲ２最大结合量（Ｂｍａｘ）Ｋｄ值。结果 与对照组（Ｃ组）相比,心衰组（Ｆ组）ＬＶＥＤＰ显著升高（Ｐ〈０．０１）,＋ｄｐ／ｄｔｍａｘ、－ｄｐ／ｄｔｍａｘ显著降低（Ｐ〈０．０１）,培哚普利组（Ｐ且）ＬＶＥＤＰ显著低于Ｆ组（Ｐ〈０．０１）ｄｐ／ｔｄｍａｘ、－ｄｐ／ｄｔｍａｘ显著高于Ｆ组（Ｐ〈０．０１）。Ｆ组心肌ＳＲ Ｃａ＾２＋泵活性、〔＾３Ｈ〕－ｒｙａｎｏｄｉｎｅ与ＲｙＲ２最大结合量Ｂｍａｘ显著低于Ｃ组（Ｐ〈０．０１）,Ｐ     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.