国产那他霉素滴眼液治疗真菌性角膜溃疡的临床研究

目的:评价国产那他霉素滴眼液与进口那他霉素滴眼液对真菌性角膜溃疡的临床疗效。方法:采用随机、对照和双盲方法随机选择2010-09/2011-03在沈阳爱尔眼科医院及哈尔滨爱尔眼科医院诊断明确的真菌性角膜溃疡患者20例20眼,以随机数字表法随机分为两组,治疗组12例应用50g/L国产那他霉素滴眼液,对照组8例应用50g/L进口那他霉素滴眼液。开始应用剂量,每30min滴眼1次共3d;如有效,则调整为每小时1次共2d;每2h1次共5d;每3h1次共2wk,疗程21～30d,两组均联合全身应用抗真菌药物。局部治疗7d若患眼体征恶化或不见好转者,更换抗真菌药物或联合其它药物治疗。分别在用药后3,5,7d对临床疗效进行评估。采用Fisher精确检验对数据进行统计学分析两组之间的疗效差异。结果:治疗组临床治疗有效率为58.3%,对照组有效率75%,Fisher精确检验结果显示组间治疗有效率无统计学差异(P>0.05)。结论:国产那他霉素滴眼液能够有效治疗真菌性角膜溃疡。     

碘酊烧灼联合那他霉素治疗真菌性角膜溃疡15例

目的 探讨碘酊烧灼联合那他霉素治疗真菌性角膜溃疡的疗效.方法 对15例真菌性角膜溃疡的患者采取碘酊烧灼联合那他霉素治疗,观察治疗效果.结果 经过碘酊烧灼联合那他霉素治疗,轻度者治愈率100%,中度者70%,重度者33%.结论 碘酊烧灼联合那他霉素对轻中度真菌性角膜溃疡治疗效果较好,对重度者效果欠佳.     

伏立康唑联合那他霉素治疗真菌性角膜溃疡

目的探讨伏立康唑全身用药联合那他霉素眼部用药治疗眼外伤后真菌性角膜溃疡的临床疗效。方法22例（22眼）真菌性角膜溃疡根据病情轻重予以口服伏立康唑首日800mg／d（400mg,2次／d）,次日400mg／d（200mg,2次／d）,疗程14—21d。联合那他霉素滴眼液滴眼,最初用药时1次／h滴眼,14d后如临床症状明显改善,可减少至6～8次／d,持续滴眼3～4周,炎症消退后逐渐减少用药频率,直至共焦显微镜提示未查及菌丝后再维持3周;角膜局部清创及碘酊烧灼,观察联合用药效果。结果22例中17例达到临床治愈（有效率为77．27％）,5例无效,其中3例行眼内容摘除术,2例行角膜移植手术治疗。所有患者均未出现严重药物不良反应。结论伏立康唑全身用药联合那他霉素眼部用药治疗真菌性角膜溃疡安全、有效,为难治性真菌性角膜溃疡提供了新的选择。     

那他霉素在真菌性角膜溃疡愈合中的治疗作用

目的:通过对那他霉素和氟康唑治疗真菌性角膜溃疡的对照研究,探讨那他霉素在真菌性角膜溃疡愈合中的治疗作用。方法:对84例确诊真菌性角膜溃疡患者随机分为甲、乙两组,每组42例,分别用那他霉素眼液及氟康唑眼液进行治疗。结果:那他霉素组治愈21例,有效34例,无效8例,有效率81%;氟康唑组治愈11例,有效29例,无效13例,有效率69%;两组有效率比较,差异无统计学意义(χ2=1.587,P>0.05);两组治愈率比较,差异有统计学意义(χ2=5.048,P<0.05),那他霉素组治愈率(50%)明显高于氟康唑组(26%);那他霉素组有效治疗时间(16.7±7.68d),明显低于氟康唑组(28.6±7.3d),两组差异显著,有统计学意义(t=7.94,P<0.01)。结论:那他霉素对真菌性角膜溃疡具有较好的治疗作用,优于氟康唑。值得临床推广使用。     

聚乙二醇滴眼液联合重组牛碱性成纤维细胞生长因子治疗干眼症

目的:评价聚乙二醇滴眼液联合重组牛碱性成纤维细胞生长因子滴眼液治疗干眼症的疗效。方法:干眼症患者51例102眼,双眼自身对照,随机分为治疗组和对照组。治疗组给予聚乙二醇滴眼液和重组牛碱性成纤维细胞生长因子滴眼液,均4次/d,两种滴眼液之间间隔5～10min。对照组给予聚乙二醇滴眼液滴眼,4次/d,连续用药1mo后复查。观察每组用药前后SchirmerⅠ试验,BUT,角膜荧光素染色和症状改善情况。结果:两组治疗前后BUT,角膜荧光素染色和症状都有显著差异(P<0.05)。两组治疗前后SchirmerⅠ试验结果无显著差异(P>0.05)。治疗后两组BUT,角膜荧光素染色和症状也有显著差异(P<0.05)。结论:聚乙二醇滴眼液联合重组牛碱性成纤维细胞生长因子滴眼液对治疗干眼症有明显的疗效。     

板层角膜切除术联合那他霉素治疗真菌性角膜溃疡的临床及病理研究

目的：研究板层角膜切除术联合那他霉素治疗真菌性角膜溃疡的临床疗效及组织病理学基础。方法：选取2009-12/2011-11真菌性角膜溃疡患者32例32眼,角膜病变未累及内皮层,行角膜板层切除联合那他霉素滴眼治疗,观察记录治疗前后视力、角膜情况、病程、并发症及致病菌种类,对切除组织行组织病理学检查。结果：治疗前视力≤0.05者20眼,～0.3者11眼,〉0.3者1眼。板层切除术后联合那他霉素治疗痊愈28例（87.5%）,治愈时间7～32（平均13.2±8.5）d,痊愈时视力≤0.05者5眼,～0.3者8眼,〉0.3者15眼。病情控制不良改行角膜移植术者4例。31例真菌培养阳性（96.9%）,其中镰刀菌18例,曲霉菌属8例,链格孢霉3例,未知菌属2例。病理学检查显示溃疡灶中有多量中性粒细胞浸润,过碘酸-希夫（PAS）染色显示角膜组织中菌丝多数位于角膜浅层,并呈与板层胶原纤维平行方向生长,但也有少数呈斜形或垂直生长方式,侵及切除组织全层。不同菌属之间菌丝在角膜中生长方式无明显差异。结论：对于病变未累及全层的真菌性角膜溃疡患者,及时行板层角膜切除术可大大提高那他霉素药物治疗有效性,缩短病程,恢复视力。     

那他霉素联合活力碘治疗真菌性角膜溃疡临床观察

目的：观察那他霉素联合5g/L活力碘治疗真菌性角膜溃疡的临床疗效。方法：对38例38眼真菌性角膜溃疡采用那他霉素联合5g/L活力碘及相关抗真菌药物（斯皮仁诺等）治疗,并进行回顾性分析。结果：经过那他霉素联合5g/L活力碘及斯皮仁诺（伊曲康唑）等药物的治疗,治愈30眼,好转7眼,1眼因角膜穿孔致真菌性眼内炎而丧失眼球。治愈率为79%,有效率97%。结论：那他霉素联合5g/L活力碘及斯皮仁诺（伊曲康唑）等药物治疗真菌性角膜溃疡是安全有效,简便实用的。     

自体血清和生物羊膜移植及那他霉素三联治疗真菌性角膜溃疡

目的:观察羊膜移植术联合自体血清及那他霉素治疗真菌性角膜溃疡的临床效果。方法:农作物及植物所致真菌性角膜溃疡80例80眼,随机分成治疗组和对照组各40例,治疗组加用自体血清。两组对比观察角膜创面愈合及角膜瘢痕形成情况。所有病例于手术显微镜下彻底清创溃疡灶及坏死的角膜组织,取瑞济生物(8mm×10mm羊膜)基地膜面贴敷于溃疡面,再取(10mm×15mm)较大的生物羊膜覆盖其上。并以10-0尼龙线缝合固定。术后治疗组加自体血清球结膜下注射。结果:局部创面全部愈合69例69眼,11例未愈。治疗组术后6mo角膜瘢痕形成情况,平均视力明显优于对照组。两者比较差异有统计学意义(P<0.01)。结论:多层羊膜移植加自体血清结膜下注射治疗真菌性角膜溃疡疗效良好。     

重组牛碱性成纤维细胞生长因子联合玻璃酸钠治疗超声乳化术后干眼

目的：探讨重组牛碱性成纤维细胞生长因子滴眼液联合玻璃酸钠对白内障超声乳化术后干眼疗效及对泪液氧化应激指标及TNF-α、IL-6水平影响。

方法：选取2016-02/2018-02我院收治的白内障术后出现干眼症的患者112例172眼作为研究对象,按照随机数字法分为两组,每组56例86眼,其中对照组给予玻璃酸钠治疗,观察组给予重组牛碱性成纤维细胞生长因子滴眼液联合玻璃酸钠治疗。对比两组患者疗效、眼表、泪液功能以及泪液中氧化应激指标和炎性因子指标。

结果：观察组治疗4wk后总有效率为96.5%,明显高于对照组总有效率80.2%(P=0.001)。两组患者术前1d的FL、BUT以及SⅠt无差异(P>0.05),治疗后两组FL均明显降低,术后治疗4、8、28d观察组下降更为明显(P<0.05); BUT和SⅠt均明显升高,术后治疗4、8、28d观察组升高更为明显(P<0.05)。治疗前两组患者泪液中氧化应激指标丙二醛(MDA)、过氧化物脂质(LPO)、超氧化物岐化酶(SOD)及总抗氧化能力(TAC)无差异(P>0.05),治疗后两组患者的氧化应激指标均明显改善(P<0.05),观察组MDA和LPO明显低于对照组(P<0.05),SOD和TAC明显高于对照组(P<0.05)。治疗前两组患者泪液中炎性因子TNF-α和IL-6无差异(P>0.05),治疗后两组TNF-α和IL-6均明显下降(P<0.05),且观察组比对照组下降更为明显(P<0.05)。

结论：重组牛碱性成纤维细胞生长因子滴眼液联合玻璃酸钠治疗白内障超声乳化后干眼效果显著,不仅能促进泪膜稳定性,还能减少泪液分泌和角膜损伤,其作用可能与改善氧化应激指标、调节炎症因子水平有关。     

那他霉素滴眼液治疗真菌性角膜炎疗效观察

真菌性角膜炎是一种致盲率很高的感染性角膜病。近年来其患病率明显增高 ,目前尚无有效的抗真菌滴眼液供临床应用。我院自 1999年 2月至 2 0 0 0年 11月共收治真菌性角膜炎189例 ,随机抽取其中 49例 (4 9眼 )用 5 %那他霉素滴眼液治疗 ,现将观察结果报告如下。1　资料与方法1.1　临床资料　 49例 (4 9眼 )均为单眼发病 ,年龄 14～ 65岁 ,平均 46岁。男 3 4例 ,女 2 5例。植物外伤史 3 0例 ,角膜异物取出史 6例 ,长期使用糖皮质激素者 3例 ,原因不明 10例。有明确外伤史、具有典型的病灶形态并反复病灶刮片查获菌丝或真菌培养阳性为确诊依据…     

Voriconazole versus natamycin as primary treatment in fungal corneal ulcers

Background: To evaluate the efficacy of topical 1% voriconazole versus 5% natamycin in treatment of fungal corneal ulcers. Design: A prospective, randomized pilot study in a tertiary care hospital. Participants: Thirty patients of microbiologically proven fungal keratitis divided randomly in two groups of 15 patients each. Methods: Two groups were treated with either 5% natamycin (group A) or 1% voriconazole (group B) topically as a primary treatment for fungal keratitis. The mean size, depth of infiltrate and LogMAR visual acuity at presentation were comparable in both groups (P > 0.05). Patients were followed up for minimum of 10 weeks or till complete resolution of ulcer, whichever was later. Cultures to identify the causative organisms were performed. Main Outcome Measure: Time of resolution of the ulcer. Results: Twenty‐nine of the total 30 patients showed complete resolution. Average time of resolution and gain in LogMAR visual acuity was 24.3 days and 1.12 in group A and 27.2 days and 0.77 in group B. These were comparable in the two groups (P > 0.05%). Aspergillus spp. (40%) and Curvularia spp. (30.0%) were found to be most common isolates. Conclusion: Topical 1% voriconazole was found to be safe and effective drug in primary management of fungal keratitis, its efficacy matching conventional natamycin. There was no added advantage of using topical 1% voriconazole over topical natamycin as primary treatment in fungal keratitis.     

真菌性角膜炎患者的临床特征及病原菌分布

目的：：分析真菌性角膜炎的临床特征及病原菌的分布,为临床诊疗提供依据。方法：回顾性分析2012-01/2015-07在长江大学附属第一医院诊治的98例98眼真菌性角膜炎患者的临床资料。结果：真菌性角膜炎最主要由植物外伤引起,其次是糖皮质激素的应用及隐形眼镜的不当使用。几乎所有患者出现菌丝苔被、伪足、免疫环、卫星灶等体征,少数患者出现内皮斑和前房积脓。真菌性角膜炎的致病菌主要以镰刀菌属为主(73.5％),其次为曲霉属(13.2％)、念珠菌属(9.2％),其他菌属4.1％。65例65眼患者在选择5％那他霉素滴眼液后治愈,15例15眼患者病情明显好转,其余18例18眼患者病情无明显好转,联合伏立康唑后13例13眼病情好转,5例5眼病情继续恶化,最终3例3眼羊膜遮盖,2例2眼眼球摘除。。结论：真菌性角膜炎在荆州地区的主要流行菌种为镰刀菌。那他霉素眼液可作为临床预防和治疗真菌性角膜炎首选药物。临床医师应加强对真菌性角膜炎的了解,以便早期诊断和及时治疗。     

无环鸟苷滴眼液联合碱性成纤维细胞生长因子滴眼液治疗单纯疱疹病毒性角膜炎疗效观察

目的 评价无环鸟苷滴眼液联合碱性成纤维细胞生长因子（重组bFGF）滴眼液治疗单纯疱疹病毒性角膜炎的疗效。方法 回顾性分析单纯疱疹病毒性角膜炎96例（96只眼）,以抗病毒药物方法治疗为对照组,共46例;抗病毒药物联合碱性成纤维细胞生长因子滴眼液为治疗组,共50例。比较两组疗效。结果两组痊愈率比较,有显著性差异（P〈0.05）。结论 无环鸟苷滴眼液联合碱性成纤维细胞生长因子滴眼液组疗效优于对照组。     

角膜胶原交联方法联合那他霉素抗真菌效果研究

目的：研究角膜胶原交联方法联合那他霉素应用时的体内外抗真菌效果,为临床真菌性角膜炎的治疗提供治疗方法及实验基础。

方法：采用黄曲霉菌、茄病镰刀菌、白色念珠菌三种常见致病真菌,实验组分为交联联合那他霉素组、那他霉素联合核黄素组、那他霉素联合紫外照射组、交联组,并用那他霉素组作为对照。将药物涂到有1.5麦氏浊度的真菌孢子悬液的沙堡弱琼脂培养皿的中心,10min后使用胶原交联仪照射10min,之后28℃培养36h,测量抑菌圈并进行统计学分析。制备兔眼茄病镰刀菌感染模型,按随机数字表法将模型兔随机分为模型对照组、交联治疗组、那他霉素治疗组、交联联合那他霉素治疗组,每组5只; 另取5只正常兔按照角膜胶原交联疗法进行照射,5只正常对照。通过前节照相、角膜刮片、共焦显微镜等观察各组治疗结果,并在治疗结束后采用电镜观察角膜超微结构改变情况。

结果：体外单独应用角膜胶原交联术,对三种真菌均无效; 当交联与那他霉素联合应用时,抗真菌效果均优于对照组,且差异具有统计学意义(P<0.05); 那他霉素与核黄素、那他霉素与紫外线分别联合应用时,抗菌效果与对照组比较,差异均无统计学意义(P>0.05)。针对兔真菌感染模型,那他霉素治疗组及交联联合那他霉素治疗组病程约14d,交联治疗组病程约21d; 经治疗后,各治疗组均已愈合,各组角膜上皮均无缺损,新生血管较多,角膜内无菌丝; 前节照相结果显示,交联联合那他霉素治疗组治疗结果优于其它各治疗组,瘢痕组织较少,角膜愈合较好,病程相对短。

结论：角膜胶原交联方法联合那他霉素,可促进角膜愈合,缩短病程,增强抗真菌效果,有望为眼科临床真菌性角膜炎的治疗提供一种新的治疗技术。     

Determination of surgical outcomes with a novel formulation of intrastromal natamycin in recalcitrant fungal keratitis: A pilot study

Purpose:To evaluate the outcomes of water-soluble intrastromal natamycin (IS-NTM) as an adjunct therapy for recalcitrant fungal keratitis.Methods:This was a prospective interventional pilot study in the setting of a tertiary eye-care center. Twenty eyes of 20 consecutive patients with microbiologically proven recalcitrant fungal keratitis (ulcer size >2 mm, depth >50%, and not responding to topical NTM for 2 weeks) were recruited. The selected patients were injected with a novel composition of IS-NTM (10 ug/0.1 mL, soluble natamycin) prepared aseptically in the ocular pharmacology department. All the patients continued using topical NTM suspension 5% 4-hourly until the ulcer healed. Repeat injections were undertaken after 72 h depending on the clinical response and all the patients were followed till 6 months.Results:The mean age of the patients was 40.42 ± 10.09 years. The mean duration of the presentation was 20.8 ± 5.1 days. The most commonly isolated organisms were Aspergillus sp. (12/20, 60%) and Fusarium sp. (8/20, 40%). No patient had iatrogenic perforation or precipitate formation after IS-NTM injection. The overall cure rate with IS-NTM was 95% (19/20 patients). The number of patients who healed with the 1^st, 2^nd, and 3^rd injection was 13, 5, and 1, respectively. One (5%) had no response to treatment and was subjected to penetrating keratoplasty. The average time taken for the resolution of the epithelial defect, stromal infiltrates, and hypopyon was 34 ± 5.2 days, 35.3 ± 6.4 days, and 15 ± 2.5 days. Healing with deep vascularization and cataract was noted in 6/19 eyes (31%) and 13/19 eyes (68.42%), respectively.Conclusion:Intrastromal injection of a novel formulation of NTM holds a promising role as adjunctive therapy to topical NTM in the management of recalcitrant filamentous fungal keratitis. The preliminary results are encouraging and further studies are required to validate the results.     

碱性成纤维细胞生长因子对人晶状体上皮细胞促增殖作用研究

研究了碱性成纤维细胞生长因子对人晶状体上皮细胞的促增殖作用。方法胎儿晶状体上皮细胞原代与传代培养。第２代培养细胞中添加ｂＦＧＦ,浓度１０＾－３－１０＾３ｎｇ／ｍｌ,用甲基噻唑基四唑测定法观察ｂＦＧＦ对ＨＬＥＣｓ的影响。结论ｂＦＧＦ在后囊混浊发生上起了生要作用。     

Evaluation of antifungal susceptibility and clinical characteristics in fungal keratitis in a tertiary care center in North India

Purpose:To study the antifungal susceptibility of common corneal pathogenic fungi to antifungal agents in the North Indian population.Methods:Prospective study of the antifungal sensitivity testing (natamycin, amphotericin B, voriconazole, itraconazole, fluconazole, posaconazole, caspofungin, micafungin) of fungal isolates from 50 cases of culture positive fungal keratitis by using E test method. Details noted included demographic data, visual acuity, clinical details, grade of keratitis, healing time, and success in medical management.Results:Of 50 patients with fungal keratitis (mean age: 40.28 ± 16.77 years), 12 eyes healed within 3 weeks, 14 had a delayed healing response, and 24 had chronic keratitis. Among the 15 cases of Fusarium isolates, 93.3% were sensitive to natamycin, while 40% to amphotericin B; 66.6% to voriconazole, 13.4% to itraconazole and fluconazole each. 80% of Fusarium cases (n = 12) showed susceptibility to posaconazole. Among Aspergillus flavus isolates, 53.4% (n = 8) were sensitive to natamycin, with only 40% (n = 7) showing sensitivity to amphotericin B and good susceptibility to azoles. MIC against susceptible Fusarium spp. for natamycin was 3–16 µg/mL, amphotericin B: 1–8 µg/mL, voriconazole: 0.5–1.5 µg/mL, itraconazole: 0.5–12 µg/mL, posaconazole: 0.094–1.5 µg/mL. MIC against Aspergillus flavus was natamycin: 8–32 µg/mL, amphotericin B: 0.5–16 µg/mL, voriconazole: 0.025–4 µg/mL, itraconazole: 0.125–8 µg/mL, posaconazole: 0.047–0.25 µg/mL; against Aspergillus niger isolates, to natamycin was 6 µg/mL (n=1), amphotericin B 8–12 µg/mL (n = 3), voriconazole: 0.125–0.19 µg/mL (n = 3), itraconazole: 0.38–0.75 µg/mL, posaconazole: 0.064–0.19 µg/mL and against Aspergillus fumigatus (n = 1), was natamycin4 µg/mL, amphotericin B - 8 µg/mL, voriconazole 0.25 µg/mL, itraconazole 1 µg/mL, and posaconazole 0.19 µg/mL. MIC against susceptible Acremonium spp. for natamycin was 1.5–16 µg/mL, amphotericin B: 0.5–8 µg/mL, voriconazole: 0.19–3 µg/mL, itraconazole: 0.125 µg/mL, posaconazole: 0.125–0.5 µg/mL and against susceptible Curvularia was natamycin 0.75–4 µg/mL, amphotericin B 0.5–1 µg/mL, voriconazole 0.125–0.19 µg/mL, itraconazole 0.047–0.094 µg/mL, posaconazole 0.047–0.094 µg/mL. MIC against Mucor spp.+ Rhizopus spp. (n = 1) was natamycin: 8 µg/mL, amphotericin B: 0.75 µg/mL, posaconazole: 1.5 µg/mL. MIC against of Alternaria (n = 1) was voriconazole: 0.19 µg/mL, posaconazole: 0.094 µg/mL. MIC against Penicillium (n=1) was natamycin: 8 µg/mL, voriconazole: 0.25 µg/mL, itraconazole: 0.5 µg/mL, and Posaconazole: 0.125 µg/mL.Conclusion:Our observations highlight the variations in susceptibility to antifungal agents. Posaconazole seems to be effective with low MIC against common corneal pathogenic fungal isolates.     

圆锥角膜组织中EGF和bFGF变化免疫组织化学的研究

目的 观察正常人及圆锥角膜中表皮生长因子(EGF)和碱性成纤维细胞生长因子（bFGF)的表达，探讨EGF和hFGF在圆锥角膜中的变化。方法 采用免疫组织化学SABC法检测角膜组织中EGF、bFGF的表达，结果 在圆锥角膜上皮层、Bowman层和内皮层有EGF表达，EGF比正常角膜的表达强；在圆锥角膜的上皮层、实质层和内皮层可见bFGF的阳性表达，圆锥角膜的基质瘢痕区bFGF有较强的阳性表达。结论 圆锥角膜组织中EGF水平增高，促进上皮细胞和基质细胞的DNA合成，从而使组织愈合。bFGF表达增强可以促进角膜上皮、基质细胞和内皮细胞的增殖。     

The Changes of TGF-α, TGF-β_1 and Basic FGF Messenger RNA Expression in Rabbit Cornea after Photorefractive Keratectomy

Objective : To study the mechanism of haze formation and investigate the expression changes of transforming growth factor-α(TGF-α), transforming growth factor-β1 (TGF-β1) and basic fibroblast growth factor (bFCF) mRNA in corneal epithelium and stroma after photorefractive keratectomy (PRK).Methods: Sixteen white rabbits were randomly divided into 4 groups, and PRK was performed on each eye of 12 rabbits. The haze formation was examined under a slit-lamp microscope at the 1st, 2nd and 3rd month after PRK, and the expressions of TGF-α , TGF-β1 and bFGF mRNA were detected with in situ hybridization.Results : The corneal haze formed at the 1" month after PRK. The most prominent haze formation was observed at the 2nd month, and declined gradually at the 3rd month after ablation. TGF-a mRNA expression was presented on the normal corneal epithelium and not on the corneal stroma. TGF-β1 and bGFG mRNA were expressed by both corneal epithelium and stroma. The capacities for cornea tissue expression of thre     

碱性成纤维细胞生长因子及其受体FGFR1在脉络膜新生血管中的表达

目的　研究碱性成纤维细胞生长因子 (bFGF)及其受体FGFR1在氪激光诱导的棕色挪威 (BN)大鼠脉络膜新生血管 (CNV)形成中的作用机制。方法　应用氪激光 ( 64 7nm ,3 60mW ,5 0 μm ,0 .0 5s)对 3 0只雄性BN大鼠实验眼进行视网膜光凝 ,分别于光凝后 3、7、14、2 1、2 8和 5 6d摘除眼球进行原位杂交检测bFGFmRNA ,免疫组织化学检测bFGF和FGFR1。结果　光凝 3d后 ,光凝区视网膜内bFGFmRNA和FGFR1蛋白表达水平逐渐下降 (P <0 .0 1)。 7d后视网膜下出现bFGFmRNA和FGFR1染色阳性的CNV ,此后其阳性染色面积及密度逐渐增加 (P <0 .0 1)。结论　bFGF和FGFR1通过自分泌和旁分泌途径在新生血管形成部位结合 ,引起FGFR1自磷酸化 ,诱导血管内皮细胞分化和CNV形成