Bf Polymorphism and its Relationship with HLA Antigens in a Sample of the Spanish Population: High BfF1 Frequencies

Bf allele frequencies were studied in a sample of the normal Spanish population and in family haplotypes. BfF1 shows a frequency higher than in other Caucasoid populations and closer to that found in Negroids. Basques show an even higher BfF1 frequency. BfF1 is in strong linkage disequilibrium with B18. HLA-Bw44 is found to be the B12 split in linkage disequilibrium with BfF and Bw50-BfS1 association is confirmed. DR3-BfF1 are not in linkage disequilibrium in the normal Spanish population, in contrast to DR3-BfF1 linkage found in a diabetic Spanish population.
Results are discussed on the bases of the paleo-North African Iberian population origins and of the use of Bf to define B12 and Bw21 splits.     

Preferential paternal transmission of the diabetogenic supratype marked by HLA B18 BfF1 DR3

Examination of transmission of the diabetogenic supratype B18 BfF1 DR3 revealed that this supratype is inherited more frequently than expected from fathers. These data suggest an explanation for increased susceptibility to development and earlier onset of insulin dependent diabetes mellitus in offspring of diabetic fathers.     

HLA-B Alleles Associated with the B15 Serologically Defined Antigens

ABSTRACT: Cells expressing HLA molecules in the B15 family were identified by serologic typing in routine testing of volunteer donors of various ethnic backgrounds for a bone marrow registry. DNA sequencing was used to identify HLA-B15 alleles associated with each serologic type and to examine the diversity within the B15 antigen family. Alleles which appeared predominantly in each B15 serologic cluster included: B15 with no defined serologic subdivision (B*1501), B62 (B*1501), B63 (B*1516, B*1517), B75 (B*1502, B*1521), and B76/77 (B*1513). Other B*15 alleles were also found associated with the serotypes and some of these alleles (e.g., B*1501 and B*1516) were found in two or more serologic clusters illustrating the complexity of this family. The B15 unsplit and B75 groups were the most complex exhibiting 16 and 7 alleles, respectively, within each serotype. Five new B*15 alleles (B*1530, B*1531, B*1533, B*1534, B*1535) and 5 other new HLA-B alleles (B*38022, B*3910, B*4010, B*51012, and B*5108) were also identified.     

胰岛素依赖性糖尿病病人补体第二途径B因子多态性的检测

用琼脂糖凝胶高压电泳继以免疫固定技术对30例胰岛素依赖性糖尿病(IDDM)病人进行了补体第二途径B因子(Bf)遗传多态性的检测。检测结果表明,IDDM病人的BfF的基因率频(0.3667)显著高于正常人(0.1159),P《0.001;且BfFF表型也较正常人显著增高,P《0.001,BfFF表型人群,IDDM发病的相对危险性(RR)为12.58。     

HLA Antigens in Patients with Psoriasis

Tissue typing was performed on lymphocytes of 46 Jewish patients with psoriasis vulgaris. HLA B13, B17, B37 and Cw6 were found increased. It is suggested that the increase in the B and C locus antigens is inter-related and may determine the genetic predisposition for the development of this disease.     

HLA Antigens and Myxedema

In 22 cases of myxedema 22 HLA-A and B antigens were typed. In six out of eight patients with a history of subacute thyroiditis Bw35 antigen was demonstrated, while in none of 14 patients free from this disease in anamnesis could this antigen be found. This difference is statistically significant, and suggests classifying of myxedema patients into two groups according to pathogenesis of the disease.     

Graves氏病人血清补体第二途径B因子遗传多态性的检测

1972年Alper等人用琼脂糖凝胶电泳加免疫固定首先发现人群中补体第二途径B因子(Properdin Factor B,Bf)呈现遗传多态性,他们当时描述了四种Bf表型,常见的S(从slow)与F(fast),及少见的SO7(slow than S)与Fl(fast than F),并提出Bf表型受常染色体共显性等位基因控制。十余年来,各国学者对Bf的多态现象进行了     

HLA-DRw Antigens Associated with Acute Leukemia

In mice, susceptibility to leukemia is closely linked to "Ia" antigens coded by the major histocompatibility complex and is transmitted recessively. To test this hypothesis in man, 89 patients with acute leukemia were typed for the eight known HLA-DRw specificities, representing the human counterpart of the Ia system, and compared with a normal unrelated control population (n=123). Phenotypes showing only one identifiable DRw antigen were significantly increased in patients with acute leukemia. Within such phenotypes, there was a predominant and significant occurrence of DRw antigens 3, 6, and 7, depending on the type of leukemia (AML, ALL in adults, and ALL in children respectively). These findings are most probably due to an excess of homozygotes for these particular DRw antigens, thus suggesting a recessive mode of transmission of susceptibility to leukemia in man.     

Human Ia-Like Antigens Associated with HLA-D

Antisera raised with HLA-A- and -B-compatible, HLA-D-disparate combinations were cytotoxic to B lymphocytes from the immunizing donor's HLA-D phenotype. Four antisera recognized structures closely associated with the HLA-D determinants Dw2, Dw3, Dw4, and LD 108. One antiserum had a broad reactivity pattern, including Dw3, Dw6, and some unknown specificity(ies). In population and family studies these B-lymphocyte antigens behaved as if they were governed by one genetic locus in the B-D region of the HLA complex. Furthermore, the antisera were cytotoxic to a minor concavalin-A-reactive T-cell subpopulation. The antisera had previously been shown to inhibit the stimulating cells in mixed lymphocyte culture and to be capable of inhibiting the Fc receptor in the EA rosette assay. We conclude that the antisera produced by this method recognize Ia-like antigens closely associated with the HLA-D determinants.     

HLA Antigens in 16 Families with Xeroderma Pigmentosum

Xeroderma pigmentosum is an autosomal recessive disease. HLA-A and -B typing was performed on peripheral blood lymphocytes and platelets. Sixteen Tunisian families were typed with 37 patients and 108 relatives. Genetic transmission of the disease and of the HLA system seemed to be independent in this study. Comparison of HLA gene frequencies between (unrelated) parents of patients and a control population showed no difference, proving that there is no clear association in populations between deleterious XP genes and a particular HLA gene. However, an excess of identical HLA among pairs of diseased siblings would suggest that the disease is polymorphic and a form of the XP could be linked to HLA.     

HLA Antigens and Monoclonal Gammapathy

Despite the strict criteria required to distinguish Multiple Myeloma (M.M.) or Waldstr?m's Macroglobulinemia (W.M.) from Benign Monoclonal Gammapathy (B.M.G.), nosological frontiers are still unclear and accordingly justify a comparative serological study of M.M., W.M., and B.M.G. patients.     

HLA A and B Locus Antigens in Patients with Unexplained. Hepatitis following Halothane Anaesthesia

HLA A and B locus antigens were determined in 17 patients who had recovered from unexplained hepatitis following halothane anaesthesia. The greatest deviations from expected frequencies were observed with A1, A11 and BW22, but these differences were not statistically significant when the P values were corrected for the number of antigens tested. Although a larger series might show such deviations to be significantly different, HLA typing is of no predictive value in determining those at risk to hepatitis following repeated halothane exposure.     

Distribution of HLA Antigens in Patients with Ewing's Sarcoma

Thirty-eight unrelated Caucasian patients with Ewing's sarcoma were typed for HLA–A and B locus antigens. Although no significant differences in the distribution of these antigens were found in comparison with local controls, a trend towards increases in HLA–B8 and Aw19 complex antigens was noted. Although American blacks are only rarely affected by this tumor, the two black patients we typed both had the HLA–Aw19 complex antigen, Aw31. This suggests that a disease susceptibility factor may be in linkage disequilibrium with HLA–Aw19 complex HLA antigens.     

HLA Antigens in Chronic Alcoholic Pancreatitis

HLA typing was performed in 90 unrelated patients with chronic alcohol-associated pancreatitis. Compared with 523 healthy controls, an increased frequency was found for the HLA-B series antigen, B40 (Pless than 0.00041, corrected P less than 0.011). The increase was slightly more pronounced in patients without pancreatic calcifications than in those with calcifications. Factors such as alcohol consumption, age of disease onset and the presence of diabetes did not affect antigen frequency distribution.     

Association of HLA Antigens with Glucose Intolerance Following Renal Transplantation

Records of 109 renal transplant patients were reviewed to test the hypothesis that post transplant glucose intolerance would be associated with "diabetic", HLA antigens (HLA--B8, B18, Bw15 and Bw16). Of 42 patients with significant glucose intolerance, 23 had one or more of these antigens (RR=2.65, P=0.01). These findings suggest that stress or drug treatment associated with renal transplantation can induce glucose intolerance in patients with a "diabetic" gene associated with the HLA--B locus.     

HLA Antigens in a Scottish Psoriatic Population

Sixty-one patients in the Dundee area suffering from psoriasis were typed for HLA-A and HLA-B antigens. On the basis of the typing results, the patients were divided into three groups, and studied with respect to sex, age of onset and familial incidence of the disease. The frequency of HLA-A1 appeared to be increased and HLA-B7 decreased but HLA-B13 and HLA-B17 were highly significantly increased (P less than 10(-6) and P less than 10(-10) respectively) in the psoriatic group compared to 204 controls. Of particular interest was a highly significant association of HLA-A1 with HLA-B17 in psoriatic patients. Family studies showed HLA-B17 to be a useful genetic marker for psoriasis in the families of B17 positive patients. Considerations of age of onset, familial incidence and typing data suggest that there is heterogeneity of genetic susceptibility to psoriasis and that one probable mechanism is the dominant inheritance of a "disease allele" in linkage disequilibrium with the allele coding for HLA-B17.