Effect of N-CAM on in vitro invasion of human adenoid cystic carcinoma cells

Adenoid cystic carcinoma of salivary glands is characterised by aggressive behaviour, high rate of local recurrences, neurotropism and late metastasis. In a previous work we demonstrated that adenoid cystic carcinoma cultured cells (CAC2 cells) expressed N-CAM. It was suggested that this expression, modulated by extracellular matrix, would be correlated to cell movement. The aim of our study was to verify whether CAC2 cells presented invasion capacity. Moreover, we tested whether the neural adhesion molecule (N-CAM) would participate in this process. CAC2 cells were either previously treated, or not (control), with a monoclonal antibody against N-CAM. Invasion assays were carried out using a modified Boyden chamber (Transwell chamber). CAC2 cells (10⁵) were dispensed into Transwell upper chamber on the top of Matrigel coated filter. The cells that invaded the filters in the first 8 h were counted under light microscopy, yielding data for the invasion rates (%). Control CAC2 cells presented an invasion rate of 5.28±0.04%. The invasion rate raised to 6.53±0.2% when N-CAM was blocked with monoclonal antibody. N-CAM impaired the adenoid cystic carcinoma cell invasion in vitro. Therefore, we suggest an anti-invasive role for N-CAM in adenoid cystic carcinoma.     

涎腺腺样囊性癌血道转移的临床分析

目的：探讨腺样囊性癌血道转移问题,期望在临床上对此类病例的治疗引起重视。方法：对６例腺样囊性癌血道转移的病人进行回顾性分析。结果：腺样囊性癌因其生物性特征,术后容易发生局部复发和血道转移。结论：对此类病例强调首期完成根治性手术和进行综合治疗。密切随访,及时发现和治疗转移灶。     

Genistein对绒毛膜癌细胞增殖、分化、细胞周期和凋亡的影响

目的：研究genistein对绒毛膜癌细胞形态学、增殖、分化、细胞周期和凋亡的影响。方法：在绒毛膜癌细胞JAR的培养基中加入不同浓度的genistein,每隔24h吸培养上清,测人绒毛膜促性腺激素(HCG)的含量。采用MTT比色法检测不同浓度genistein对JAR细胞增殖的影响。采用HE染色、电镜和流式细胞术检测JAR细胞经genistein处理72h,细胞形态学和超微结构的变化以及genistein对细胞周期和凋亡的影响。结果：Genistein能抑制绒毛膜癌细胞JAR的增殖和诱导凋亡。100nmoI/mL genistein使JAR细胞HCG分泌量增加,并呈时间依赖性；与对照组G_2/M为(17.44±6.1 6)%比较,100 nmoL/m1L genistein使细胞周期阻滞在G_2/M期,G_2/M期比例为(67.14±1.02)%；Annexin-V和PI双染色提示100nmol/L genistein作用JAR细胞72h后,能导致大部分细胞发生晚期凋亡和坏死。结论：Genistein能抑制肿瘤细胞增殖,诱导分化和凋亡,导致细胞周期阻滞。     

头颈部唾液腺腺样囊性癌术后调强放疗预后分析

目的:探讨头颈部唾液腺腺样囊性癌(ACC)的综合治疗模式及预后因素。方法:收集2016—2018年间上海交通大学医学院附属第九人民医院放疗科收治的头颈部唾液腺ACC完成术后放疗患者资料进行回顾性分析。 Kaplan- Meier法生存分析, log- rank检验单因素预...     

食管原发性腺样囊性癌的临床病理分析

目的 探讨食管腺样囊性癌（ACC）的临床病理特征、组织来源及预后。方法 应用HE染色、免疫组化及组织化学染色，对3例食管腺样囊性癌进行病理及临床分析。结果3例食管腺样囊性癌的癌组织主要由立方形腺上皮细胞和变异的肌上皮细胞组成，呈筛孔状腔隙、管状、实体状排列，腔隙内含嗜碱性和嗜伊红分泌物；3例食管腺样囊性癌中例1表面上皮有移行；免疫组化：3例CK、H-CK、P63、S-100（浆，核）、EMA、Bcl-2均（＋），例3CerBb-2（±），P53（＋），例2CD117（＋），AAT（±）、SMA（-），PAS／AB（＋）。结论 发生在食管的腺样囊性癌很少见，诊断标准及组织细胞起源建议严格定义，起源于唾腺的诊断腺样囊性癌，凡起源于其它部位或组织者应冠以腺样囊性癌。     

涎腺腺样囊性癌增殖、侵袭及转移相关信号通路的研究进展

涎腺腺样囊性癌是最常见的涎腺恶性肿瘤之一,其生长缓慢、侵袭性强,常沿骨膜和神经扩散,早期即可发生远处转移.涎腺腺样囊性的侵袭和转移是一个多阶段、多因素参与的过程,涉及复杂的机制和多条信号转导通路.文章就相关信号通路的研究进展进行综述.     

13例原发性颈段气管腺样囊性癌的手术治疗

国内外很少报道原发性颈段气管腺样囊性癌的外科治疗,本研究探讨13例该类患者外科治疗的有效性。     

Inhibition of mTOR reduce Stat3 and PAI related angiogenesis in salivary gland adenoid cystic carcinoma

Angiogenesis is a complex biological process, which is involved in tumorigenesis and progression. However, the molecular mechanism of underlying angiogenesis remains largely unknown. In this study, we accessed the expression of proteins related angiogenesis by immunohistochemical staining of human tissue microarray which contains 72 adenoid cystic carcinoma (AdCC), 12 pleomorphic adenoma (PMA) and 18 normal salivary gland (NSG) using digital pathological scanner and scoring system. We found that the expression of p-S6^S235/236 (a downstream molecule of mTOR), p-Stat3^T705, PAI, EGFR, and HIF-1α was significantly increased in AdCC as compared with PMA and (or) NSG (p < 0.05). While, the expression of these proteins was not associated with pathological type of human AdCC (p > 0.05). Correlation analysis of these proteins revealed that p-S6^S235/236 up-regulates the expression of EGFR/p-Stat3^T705 (p < 0.05) and HIF-1α/PAI (p < 0.05). Moreover, the activation of p-S6^S235/236, EGFR/p-Stat3^T705 and HIF-1α/PAI associated with angiogenesis (CD34) and proliferation (Ki-67). In vitro, Rapamycin suppressed the expression of p-S6^S235/236, EGFR, p-Stat3^T705, HIF-1α and PAI. Further more, target inhibition of mTOR by rapamycin effectively reduced tumor growth of SACC-83 cells line nude mice xenograft and decreased the expression of p-S6^S235/236, EGFR/p-Stat3^T705 and HIF-1α/PAI. Taken together, these data revealed that mTOR signaling pathway regulates tumor angiogenesis by EGFR/p-Stat3^T705 and HIF-1α/PAI. Inhibition of mTOR by rapamycin could effectively reduced tumor growth. It is likely that mTOR inhibitors may be a potential candidate for treatment of AdCC.     

Inhibition of STAT3 reduces proliferation and invasion in salivary gland adenoid cystic carcinoma

In this study, we accessed the expression and correlation of p-STAT3 with Survivin, Cyclin D1, CD147, Slug and Ki67 by immunohistochemical staining of human tissue microarray which contains 72 adenoid cystic carcinoma (AdCC), 12 pleomorphic adenoma (PMA) and 18 normal salivary gland (NSG) using digital pathological scanner and scoring system. We found that the expression of p-STAT3, Survivin, Slug, Cyclin D1 and CD147 was significantly increased in AdCC as compared with PMA and (or) NSG (p<0.05). While, the level of p-STAT3 and expression of Cyclin D1 and CD147 was not associated with pathological type of human AdCC (p>0.05). Correlation analysis of these proteins revealed that p-STAT3 up-regulates the expression of Survivin, Slug, Cyclin D1 and CD147 (p<0.05). Moreover, the activation of STAT3 was associated with proliferation marker Ki-67 (p<0.05). Selective inhibition of STAT3 by a small molecule S3I-201 significantly reduced human SACC-83 and SACC-LM cells proliferation, migration and invasion with the corresponding decrease in expression of Survivin, Slug, Cyclin D1 and CD147. These findings indicate that high phosphorylation level of STAT3 in AdCC is related to Survivin, Slug, Cyclin D1 and CD147. We suggest that the inhibition of STAT3 may be a novel strategy for neoadjuvant chemotherapeutic treatment of AdCC.     

High-grade adenoid cystic carcinoma originating from the lacrimal gland

Among primary lacrimal gland tumors, adenoid cystic carcinoma (ACC) is the most common malignant epithelial neoplasm; it is characterized by local intracranial invasion. A case with unusual dumbbell-type intracranial extension representing cavernous sinus syndrome is described. A 49-year-old woman was admitted to our hospital with right cavernous sinus syndrome. Computerized tomographic (CT) scans and magnetic resonance (MR) imaging demonstrated well-enhanced intraorbital and middle fossa tumors mimicking multifocal mass lesions. Operative findings revealed an ACC originating from the lacrimal gland and extending into the right cavernous sinus and middle fossa along the nerve sheath in the superior orbital fissure. Although MR image findings of intracranial ACC often resemble the image findings for meningiomas, intracranial ACC is very aggressive in comparison with meningioma. It is best treated surgically and aggressively.     

126例涎腺腺样囊性癌的疗效及预后因素

目的　总结头颈部涎腺腺样囊性癌的治疗效果,评价影响局部复发和生存的因素。方法　回顾性分析126 例涎腺腺样囊性癌的临床资料。结果　总的局部复发率为35 % 。放射治疗有效率为80 % 。总的5,10 及20 年累积生存率分别为81.7% ,63 .8% 及37 .0% ;无局部复发者5,10 及20 年累积生存率分别为87 .6% ,77 .0% 及73 .1% ;总的5,10 及20 年局部控制率分别为85.7% ,60.1% 及29.4 % 。区域淋巴结转移率为10 % ,肺转移率为24 % 。分析结果表明:治疗方式、病理类型及外科切缘与局部复发有着明显的关系,病理类型、外科切缘及区域淋巴结转移对生存有明显的影响,而肿瘤部位和临床分期对局部复发和累积生存均无影响。结论　涎腺腺样囊性癌发展病程较慢,外科是主要的治疗方式,放射治疗可以有效地控制病变的发展,对局部病变较晚和外科切缘阳性的患者需手术加放射治疗的综合治疗     

嗜神经侵袭阳性鼻腔副鼻窦腺样囊性癌的治疗及预后分析

目的分析嗜神经侵袭(PNI)阳性鼻腔副鼻窦腺样囊性癌(ACC)的疗效, 探讨PNI在鼻腔副鼻窦ACC中的预后价值。方法回顾性分析2000年1月至2016年12月中国医学科学院肿瘤医院收治的105例鼻腔副鼻窦ACC患者的临床资料。按照美国癌症联合委员会和国际抗癌联盟第8版分期系统重新分期。随访获取患者治疗失败和生存资料, 预后影响因素的单因素分析采用Log rank检验, 多因素分析采用Cox回归比例风险模型。结果上颌窦(59例)为最常见的肿瘤原发部位, 其次为鼻腔(38例)。Ⅲ～Ⅳ期患者93例。治疗方式包括单纯手术14例, 单纯放疗13例, 术前放疗+手术10例, 手术+术后放疗68例。中位随访91.8个月, 5年局部控制率、无远处转移生存率、无进展生存率和总生存率分别为72.6%、73.0%、52.9%和78.0%。33例(31.4%)患者PNI阳性, 其5年无远处转移生存率、无进展生存率和总生存率分别为53.7%、29.4%和56.5%, 均低于PNI阴性组(分别为80.8%、63.0%和86.8%, 均P<0.05), 而两组的局部控制率差异无统计学意义(64.5%和76....     

气管腺样囊性癌八例临床分析

背景与目的 气管腺样囊性癌是一种少见的原发于气管的低度恶性肿瘤,临床易误诊、漏诊.本研究拟提高对气管腺样囊性癌的认识.方法 回顾我院2001年至2007年经组织病理学证实的8例气管腺样囊性癌的临床表现、治疗、随访.结果 患者的主要临床表现为咳嗽、咯血、喘鸣及进行性的呼吸困难.CT及纤维支气管镜检查有助于明确诊断.5例患者行手术治疗;3例行纤维支气管镜下高频电切术治疗,1例复发后再行手术;2例术后行化疗.1例失访;1例电切术后11个月死亡;余6例随访2～34个月均健在.结论 气管腺样囊性癌恶性程度低,患者预后好.手术为首选治疗.内镜下治疗为局部姑息性治疗,可缓解症状、提高生活质量.     

64例大涎腺腺样囊性癌的预后因素分析

背景与目的:涎腺腺样囊性癌是一类恶性程度高的涎腺肿瘤,针对大涎腺来源的腺样囊性癌的研究较少。本研究旨在探讨影响大涎腺腺样囊性癌患者预后的因素。方法:回顾性分析1971年1月至1998年7月中山大学肿瘤防治中心收治并随诊7年以上的64例大涎腺腺样囊性癌患者的临床资料,应用Kaplan-Meier法进行生存分析,组间比较用log-rank检验,多因素分析采用Cox模型进行分析,两组比率的比较采用χ2检验。结果:本组总死亡率为57.8%,肿瘤相关死亡率为46.9%;5年、10年累积生存率分别为65.6%和54.5%;复发率为34.4%,远处转移率为45.0%;单因素分析结果显示,年龄、神经是否受侵、病理类型、临床分期、手术切缘是否干净是影响患者预后的因素。年龄≥50岁、神经受侵、病理类型为实体型或小管型、临床分期为晚期(Ⅲ、Ⅳ期)、切缘阳性者的预后较年龄<50岁、神经未受侵、病理类型为筛孔状、临床分期为早期(Ⅰ、Ⅱ期)、切缘阴性者差;多因素分析结果显示,神经受侵、病理类型为实体型、临床分期属于晚期(Ⅲ、Ⅳ期)、切缘阳性者,预后差。结论:神经是否受侵、病理类型、临床分期、手术切缘是否干净是影响大涎腺腺样囊性癌患者预后的独立因素。     

NOTCH1 signaling contributes to cell growth,anti-apoptosis and metastasis in salivary adenoid cystic carcinoma

Background: Numerous studies have reported both the tumor-suppressive and oncogenic roles of the Notch pathway, indicating that Notch activity regulates tumor biology in a complex, context-dependent manner. The aim of the present study was to identify the role of NOTCH1 in the cell growth and metastasis of SACC.Methods: We analyzed the expression of NOTCH1 in clinical SACC samples using immunohistochemical staining. We silenced the expression of NOTCH1 and overexpressed activated NOTCH1 to elucidate the effects of NOTCH1 on proliferation, migration and invasion. NOTCH1 target genes were validated by real-time PCR.Results: Our results showed that NOTCH1 was upregulated in SACC tissues when compared with normal tissues, and this upregulation was further enhanced in SACC tissues with metastasis and recurrence when compared with SACC tissues without metastasis. Overexpression of NOTCH1 in SACC cells promoted cell growth, migration and invasion, and knockdown of NOTCH1 inhibited cell proliferation in vitro and tumorigenicity in vivo by inducing cell apoptosis.Conclusions:The results of this study suggest that NOTCH1 plays a key role in the cell growth, anti-apoptosis, and metastasis of SACC. NOTCH1 inhibitors might therefore have potential therapeutic applications in treating SACC patients by inhibiting cancer cell growth and metastasis.