定向诱导大鼠骨髓间充质干细胞分化为成骨细胞

目的建立体外定向诱导大鼠骨髓间充质干细胞（MSCs）分化为成骨细胞的模型,为将MSC3用作骨组织工程的种子细胞奠定基础。方法用成骨添加剂（地塞米松10^-8mol/L、β-甘油磷酸钠10mmol／L、维生素C50mg/L）定向诱导传代大鼠骨髓MSCs分化为成骨细胞,通过形态学、生长曲线、免疫细胞化学及碱性磷酸酶染色鉴定成骨细胞。结果诱导组具有成骨细胞的形态和生长特点,增殖相对缓慢,但与对照组间的差异没有统计学意义（P〉0．05）,碱性磷酸酶活性增强。结论建立了体外定向诱导大鼠骨髓MSCs向成骨细胞转化的模型,成骨添加剂不影响细胞的增殖,可迅速大量获得种子细胞。     

间充质干细胞迁移中的信号通路

间充质干细胞因具有多向分化,来源广泛、取材容易和便于自体移植等特点,是一种理想的组织工程干细胞.国内外有关其用于治疗的研究已取得了一定的进展,但是其具体的作用机制仍不是很清楚.近来有关其信号通路的研究较多,本文就其中有关间充质干细胞迁移的相关通路,如PI-3K/AKT信号通路、MAPK/ERK1/2信号通路、Wnt3a信号通路、Jak/STAT信号通路、RhoA-Rho kinase信号通路、SMAD信号通路,进行综述,以便更全面的理解间充质干细胞迁移的机制.     

富血小板血浆促进骨髓间充质干细胞的增殖

背景：富血小板血浆含有多种刺激因子,且能上调蛋白多糖及胶原合成。目的：观察山羊富血小板血浆对山羊骨髓间充质干细胞增殖的影响。方法：抽取6月龄的内蒙古锡盟山羊颈静脉血,采用二度离心法获取富血小板血浆,运用骨髓穿刺包从山羊髂骨上抽取骨髓,采用密度梯度离心方法分离、纯化获得山羊骨髓间充质干细胞,然后取生长状态较好的原代细胞,加入含体积分数为10%,20%,30%富血小板血浆的L-DMEM完全培养基进行培养,对照组只用L-DMEM完全培养基培养。结果与结论：在2-6 d期间,富血小板血浆各组明显比对照组细胞增殖迅速,而且体积分数越高细胞生长的速度越快,生长的数目越多,细胞形态越成熟。培养第4天时,体积分数为10%,20%,30%富血小板血浆组细胞倍增时间约为50 h,35 h,25 h。结果表明山羊富血小板血浆能明显促进骨髓间充质干细胞的增殖,而且富血小板血浆体积分数越大促增殖能力越显著。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

小鼠骨髓间充质干细胞生物学特性和体外诱导分化

目的研究小鼠骨髓间充质干细胞的生物学性状和多系分化潜能。方法取Balb／c小鼠骨髓单个核细胞在低糖的培养液中培养出贴壁生长的细胞,进行形态学观察、细胞周期和免疫表型分析;在不同的因子作用下诱导向成骨细胞、软骨细胞,脂肪细胞分化,并检测诱导后细胞相应的基因表达。结果小鼠骨髓间充质干细胞贴壁生长后形态较均一,增殖能力随着传代逐渐增强,但从第8代后增殖能力明显减退。细胞表达CD29,CD38,CD44,CD106等标记,但CD34和H-2k表达阴性。在不同的诱导培养体系里间充质干细胞能分化为成骨细胞、软骨细胞和脂肪细胞,相应的骨钙蛋白基因,Ⅱ型胶原基因,脂蛋白脂酶基因表达都明显增强。结论从小鼠骨髓可以分离培养出间充质干细胞,在体外有效扩增和诱导分化。表明可以以小鼠为模型研究间充质干细胞在组织工程、细胞移植、基因治疗等领域的运用。     

成年大鼠骨髓间充质干细胞的体外分离

目的应用全骨髓贴壁法和密度梯度离心法以不同培养条件,探讨体外获得高质量、高活性的成年大鼠骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)的方法。方法取成年雄性SD大鼠双侧股骨,应用全骨髓贴壁法,分为10%国产TBD胎牛血清+DMEM组、10%国产TBD胎牛血清+DMEM/F12组、12.5%国产TBD胎牛血清+DMEM/F12组、10%进口以色列胎牛血清+DMEM/F12组4组,3只/组;应用密度梯度离心培养法,以10%进口以色列胎牛血清+DMEM/F12作为培养基,取3只大鼠作为一组。比较不同培养方法及培养条件对BMSCs生长增殖的影响。结果全骨髓贴壁法中10%进口以色列胎牛血清+DMEM/F12组原代细胞即可7～9 d达融合,传代到第4代细胞活力仍很好。其余3组原代细胞达融合的时间均超过10 d,且传代后细胞活力差,容易出现老化。密度梯度离心培养法原代贴壁细胞太少,无法达融合。结论本实验采用的培养方法中,10%进口以色列胎牛血清+DMEM/F12培养基的全骨髓贴壁分离法,是体外获取成年大鼠BMSCs最佳方法。     

骨髓间充质干细胞Notch1信号通路异常与骨髓瘤骨病

背景：多发性骨髓瘤骨病的发病机制目前尚未完全明确,骨髓间充质干细胞向成骨细胞分化障碍参与其中,而Notch1信号通路在间充质干细胞的增殖分化中起重要作用。 目的：探讨Notch1信号通路在多发性骨髓瘤骨病中的作用。 方法：分离培养多发性骨髓瘤患者和正常人骨髓间充质干细胞,Real-time PCR和Western blot检测成骨诱导分化前后Notch1和成骨基因Runx2的表达,以及Von Kossa染色鉴定钙质沉积程度。在多发性骨髓瘤患者间充质干细胞成骨诱导分化过程中,加入Notch1信号通路抑制剂DAPT和安慰剂,48 h后real-time PCR和western blot鉴定Notch1信号通路下游分子Hes1和成骨指标Runx2表达,2周后Von Kossa染色鉴定钙质沉积程度。 结果与结论：成骨诱导48 h后,间充质干细胞的Notch1表达减低,但是骨髓瘤患者间充质干细胞的降低幅度小于正常对照间充质干细胞;48 h后Runx2的表达在骨髓瘤患者间充质干细胞的表达明显弱于正常对照间充质干细胞;2周后,Von Kossa染色鉴定钙质沉积程度,骨髓瘤患者间充质干细胞明显弱于正常对照间充质干细胞;48 h后Hes1表达在DAPT组明显低于安慰剂组;而Runx2的表达在DAPT组明显高于安慰剂组。2周后 DAPT组钙质沉积明显强于安慰剂组。实验说明多发性骨髓瘤患者的间充质干细胞中,Notch1信号通路失活缺陷可能抑制其向成骨细胞分化。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

骨髓间充质干细胞自体移植促进缺血肢体血管生成的实验研究

目的探索骨髓间充质干细胞在血管生成中的作用,为临床建立一种简便安全的下肢动脉缺血性疾病的治疗途径。方法24只新西兰兔分为实验组(12只)和对照组(12只),分离培养实验组兔骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs),Brdu标记,建立兔后肢缺血动物模型,将取于自体的骨髓间充质干细胞制成悬液注射于实验组兔缺血部位,对照组注射等量生理盐水。2周后行实验组和对照组兔股动脉的二维及彩色多普勒超声检测,观察移植前后的血管内径,血流峰值速度,血流加速时间等;取缺血部位肌肉组织,免疫荧光染色观察移植细胞分布及血管生成情况。结果细胞移植后2周,实验组的股动脉内径,血流峰值速度,加速时间均大于对照组,有显著性差异(P<0.01);免疫荧光染色结果显示移植部位有移植细胞存在,移植组血管生成情况明显优于未移植组。结论骨髓间充质干细胞具有促进血管生成的作用,自体移植可望成为一种简单的、有效的治疗下肢缺血的方法。     

SDF-1/CXCR4信号通路参与骨形态发生蛋白2在诱导骨髓间充质干细胞迁移的作用北大核心

背景：SDF-1/CXCR-4生物轴对多种细胞的趋化能力,使其在多种组织的再生过程中发挥着重要的调控作用,但其是否参与介导骨形态发生蛋白2在骨修复过程中骨髓间充质干细胞归巢目前尚不清楚。目的：探索SDF-1/CXCR4信号通路在骨形态发生蛋白2诱导小鼠骨髓间充质干细胞迁移中的作用。方法：取对数生长期骨髓间充质干细胞,分别以0,50,100,200μg/L SDF-1,0,50,100,200μg/L骨形态发生蛋白2以及50μg/L AMD3100进行干预,诱导细胞迁移并Transwell检测。结果与结论：骨髓间充质干细胞的迁移数量与SDF-1和骨形态发生蛋白2密切相关,并且与SDF-1和骨形态发生蛋白2的浓度成正比,当联合使用SDF-1和骨形态发生蛋白2时,细胞迁移数较单独使用时增多,当质量浓度为200μg/L时,骨髓间充质干细胞迁移数量最多,并呈＂巢穴＂样分布。阻断剂AMD3100明显抑制骨形态发生蛋白2诱导骨髓间充质干细胞迁移,但当增加骨形态发生蛋白2浓度达到一定量时,骨髓间充质干细胞迁移数随着骨形态发生蛋白2浓度的增加而增多。提示SDF-1/CXCR4信号通路参与骨形态发生蛋白2诱导骨髓间充质干细胞的迁移。     

骨髓间充质干细胞诱导成骨的研究进展

骨髓间充质干细胞因其具有自我复制和多向分化潜能而成为目前骨组织工程领域研究的重点之一。对这方面近几年的研究成果从诱导因子的作用及调控机制、物理条件、新的研究方法等方面做了简要论述。     

大鼠骨髓间充质干细胞的分离和体外培养

骨髓中的间充质干细胞 (ＭＳＣｓ ,ｍｅｓｅｎｃｈｙｍａｌｓｔｅｍｃｅｌｌｓ)是一种具有多向分化潜能的细胞 ,它的分化程度低 ,在特定的条件下 ,能诱导分化形成多种非造血组织细胞 (如 :成骨细胞、软骨细胞、肌细胞等 )。本实验对成体大鼠骨髓间充质干细胞进行了分离、培养和初步的组织化学检测 ,并对其生长方式进行了观察。1　材料与方法1.1　材料　实验动物为成年ＳＤ大鼠 ,由广西医科大学实验动物中心提供 ,饲养观察一周后使用。主要试剂为ＤＭＥＭ培养液 ,胎牛血清 ,胰蛋白酶和Ｐｅｒｃｏｌｌ分离液。1.2　方法1.2 .1　骨髓中间充质干…     

骨髓间充质干细胞向软骨及骨分化：Wnt5a/PCP信号通路作用的研究与进展

BACKGROUND: Wnt5a is able to inhibit canonical Wnt signaling and activate non-canonical Wnt signaling pathway. In recent years, it has been found that non-classical Wnt5a/PCP signaling pathway mediated by Wnt5a plays an important role in the process of bone marrow mesenchymal stem cell proliferation and differentiation, but the underlying mechanism is unclear. OBJECTIVE: To summarize the progress in downstream effector molecules related to Wnt5a/PCP signaling pathway, and its roles in the chondrogenic and osteogenic differentiation of bone marrow mesenchymal stem cells. METHODS: A computer-based online search of CqVip, CNKI and PubMed databases between January 2000 and February 2016 was performed using the Chinese keywords of “BMSCs, Wnt signaling pathways, chondrogenic differentiation, osteogenic differentiation” and English keywords of “BMSCs, chondrogenic differentiation, osteogenic differentiation, Wnt, Fzd, Ror2, RhoA, ROCK, JNK”, respectively. Literatures related to bone marrow mesenchymal stem cell chondrogenic and osteogenic differentiation were selected. Finally, 43 eligible articles were included for analysis through excluding the old and repeated research. RESULTS AND CONCLUSION:Wnt5a, a representative protein in non-canonical Wnt signaling pathway, paticipates in the cytoskeleton, cell migration and cell polarization and other activities by mediating its downstream signaling molecules such as Fzd, Ror, RhoA, ROCK, JNK, thereby regulating its proliferation and differentiation. But it is unclear how Wnt5a/PCP participates in the bone marrow mesenchymal stem cell chondrogenic and osteogenic differentiation and how the downstream effector molecules interact or function independently, which requires further studies. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

Wnt信号途径促进脂肪间充质干细胞向Ⅱ型肺泡上皮细胞分化

背景：脂肪间充质干细胞向Ⅱ型肺泡上皮细胞定向分化的能力以及调节机制尚未完全阐明。 目的：观察脂肪间充质干细胞在体外分化为Ⅱ型肺泡上皮的能力以及Wnt途径对分化的调节作用。 方法：取大鼠脂肪组织,体外分离培养脂肪间充质干细胞并通过流式细胞术进行鉴定。实验分为对照组、小气道生长液组和Wnt3a组,对照组用普通DMEM培养基培养,小气道生长液组和Wnt3a组均使用小气道生长液培养,且Wnt3a组加入Wnt信号通路激动剂Wnt3a培养。诱导10 d后分别通过qRT-PCR和免疫荧光检测Ⅱ型肺泡上皮标志物肺表面活性蛋白B,C,D的表达,并于诱导5 d和10 d时通过Western blot检测磷酸化β-catenin和GSK-3β。 结果与结论：大鼠脂肪组织中可成功分离出纯度较高的脂肪间充质干细胞,可表达CD44和CD29,不表达CD11b和CD45;经小气道生长液诱导后,脂肪间充质干细胞中肺表面活性蛋白B,C,D蛋白和mRNA表达均上调(P < 0.01),表明其可被诱导为Ⅱ型上皮细胞;加入Wnt3a后,经诱导的脂肪间充质干细胞中肺表面活性蛋白B,C,D蛋白和mRNA表达均高于小气道生长液组(P < 0.01),且在诱导过程中磷酸化β-catenin表达随时间逐渐上调而GSK-3β表达逐渐下调(P < 0.01)。结果证实,Wnt信号通路在脂肪间充质干细胞诱导分化为Ⅱ肺泡上皮细胞过程中激活并促进干细胞的定向分化。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程      

Co-transplantation of human mesenchymal stem cells promotes human CD34+ cells engraftment in a dose-dependent fashion in NOD/SCID mice

Mesenchymal stem cells (MSCs) have recently been identified and characterized in humans. Moreover, MSC secrete cytokines that can support hematopoietic progenitor growth. In the present study, we evaluated whether the efficacy of hematopoietic stem cell transplantation is improved by their co-transplantation with MSC, and whether this is positively correlated with the dose of infused MSCs. Accordingly, irradiated NOD/SCID mice were transplanted with 1 x 10(5) human CD34+ cells in the presence or absence of culture expanded MSCs (1 x 10(6) or 5 x 10(6)). We evaluated human hematopoietic cell engraftment by flow cytometry and assessed MSC tissue distributions by fluorescence in situ hybridization. We found that CD45+ and CD34+ cell levels were significantly elevated in a dose-dependent manner in co-transplanted mice 4 weeks after transplantation. The engraftments of CD33+ and CD19+ cells also increased dose-dependently. However, the engraftment of CD3+ cells did not increase after co-transplantation with MSCs. Human Y chromosome+ cells were observed in multiple tissues and were more frequently observed in mice co-transplanted with 5 x 10(6) rather than 1 x 10(6) MSCs. These results suggest that MSCs are capable of enhancing hematopoietic cell engraftment and distribution in multiple organs in a dose-dependent fashion.     

胎盘间充质干细胞的应用研究

背景：胎盘间充质干细胞因其具有来源广泛、免疫原性低、不涉及伦理问题等优点成为种子细胞的新来源。 目的：阐述胎盘间充质干细胞的来源、生物学特性及应用最新研究进展。 方法：检索 PubMed、ScienceDirect、OvidSP、CNKI 数据库相关文章,检索时限为2003至 2015年,英文检索词为“Placenta,Mesenchymal stem cells,The placenta mesenchymal stem cells, Cell transplantation , Application mechanism”,中文检索词为“胎盘,间充质干细胞,胎盘间充质干细胞,细胞移植,应用机制”,从中筛选出与主题相关且论据可靠的部分文献,最终纳入57篇文章进行归纳综述。 结果与结论：目前已成功分离培养出胎盘间充质干细胞,并对其生物学特性进行研究,证明其具有干细胞源性及多向分化潜能。目前有较多关于胎盘间充质干细胞应用于实验动物及临床的研究,在骨组织工程、血管再生及神经组织等修复过程中均显示出了巨大的潜力,但胎盘间充质干细胞的具体应用机制目前尚不清楚,尚处于探索阶段,在胎盘间充质干细胞广泛应用于临床之前,仍有许多问题有待进一步研究明确,以确保其安全性和有效性。  中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

Mesenchymal stem cells improve wound healing in vivo via early activation of matrix metalloproteinase-9 and vascular endothelial growth factor

We investigated the effects of mesenchymal stem cells (MSCs) on wound healing using a three-dimensional (3D) collagen gel scaffold. Three circular full-thickness skin defects were created on the back of Sprague-Dawley rats. One site was covered with a 3D collagen gel containing 2 × 10(6) MSCs (MSCs+/3D collagen+). Another site was replaced with a 3D collagen gel without MSCs and the third site was left empty. The wound size was significantly reduced in the MSCs+/3D collagen+ sites. MSCs+/3D collagen+ sites exhibited the most neovascularization. FISH showed that Y-chromosome possessing cells were found within the dermis of MSCs+/3D collagen+ sites. Gelatin zymography revealed that the most intense expression of MMP-9 was detected early in the MSCs+/3D collagen+ sites. Our results indicate that MSCs upregulate the early expression of MMP-9 which induces the early mobilization of VEGF. Thus, MSCs appear to accelerate significantly wound healing via early activation of MMP-9 and VEGF.     

间充质干细胞条件培养液对正常成纤维细胞及瘢痕成纤维细胞转化生长因子β产生和信号通路的影响

BACKGROUND: Numerous studies have shown that mesenchymal stem cells (MSCs) can effectively attenuate the fibrosis of damaged heart, lung and kidney by secreting various bioactive factors. OBJECTIVE:To evaluate the anti-fibrotic therapeutic potential of bone marrow MSCs conditioned media in vitro. METHODS: Normal fibroblasts and hypertrophic scar fibroblasts were treated with bone marrow MSCs conditioned media, then transforming growth factor-β and collagen production were analyzed by ELISA, and mRNA expression level of Smad7 and hydroxyproline content were detected by RT-PCR and colorimetry, respectively. RESULTS AND CONCLUSION: Bone marrow MSCs conditioned media significantly inhibited the production of both transforming growth factor-β and collagen in hypertrophic scar fibroblasts (P < 0. 01), and up-regulated the mRNA expression level of Smad7 (P < 0. 01), a major inhibitory regulator in the SMAD family. However, the normal fibroblasts were scarcely influenced by bone marrow MSCs conditioned media. These findings indicate that bone marrow MSCs conditioned media is considered a promising candidate for the treatment of hypertrophic scars, which may provide new theoretical supports to reduce cutaneous scarring. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

骨髓间充质干细胞与膀胱脱细胞基质支架的生物相容性

背景：国内外许多研究者一直寻找理想的种子细胞与合适的支架材料复合,试图模拟接近正常生理功能的组织工程化尿路替代物。 目的：探讨骨髓间充质干细胞与兔膀胱脱细胞基质支架的生物相容性。 方法：采用密度梯度离心法分离培养兔骨髓间充质干细胞,将第3代兔骨髓间充质干细胞接种到兔膀胱脱细胞基质上进行复合培养,每天进行细胞计数,连续12 d,绘制细胞生长曲线,以单独培养骨髓间充质干细胞为对照组。 结果与结论：骨髓间充质干细胞成功种植到膀胱脱细胞基质上,倒置显微镜下可见骨髓间充质干细胞从膀胱脱细胞基质边缘爬出,膀胱脱细胞基质周围有大量长梭形骨髓间充质干细胞生长。接种5 d内,两组细胞均呈现出平缓生长的状态,接种6-9 d,生长曲线逐渐变得陡峭,细胞呈倍数状态进行分裂生长,速度较快,接种10-12 d,再次趋于平缓状态。两组细胞生长曲线基本重合,可推断兔骨髓间充质干细胞与膀胱脱细胞基质生物相容性良好。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

血管内皮生长因子165转染可促进脂肪间充质干细胞增殖

背景：血管内皮生长因子是目前最重要的促血管新生因子之一,在脂肪移植中可促进移植物的血运重建,提高成活率。 目的：探讨血管内皮生长因子165基因转染促进脂肪间充质干细胞增殖的作用。 方法：利用目的片段重组血管内皮生长因子165基因入腺病毒pAdEasy-1系统,包装病毒并测定滴度,同理包装空病毒。将包装成功的两种病毒液分别以100的最佳感染复数转染入脂肪间充质干细胞内,以未转染细胞为空白组。采用RT-PCR和Western blot法检测各组转染细胞中血管内皮生长因子165 mRNA和蛋白的表达,MTT法检测各组细胞的增殖情况。 结果与结论：实验组血管内皮生长因子165 mRNA和蛋白表达量高于对照组和空白组(P < 0.05);实验组转染脂肪间充质干细胞后分裂增殖程度显著增加,与对照组和空白组比较差异有显著性意义(P < 0.05),结果表明腺病毒介导的血管内皮生长因子165基因转染脂肪间充质干细胞后可以持续表达目的蛋白,同时也能促进脂肪间充质干细胞的显著增殖。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

重组腺病毒介导突变型低氧诱导因子1α基因转染脂肪间充质干细胞并促进其增殖

BACKGROUND:To improve the survival rate of transplanted tissue, most scholars focus on cell therapy, particularly cell-assisted fat grafting. OBJECTIVE:To analyze the effect of recombinant adenovirus-mediated hypoxia inducible factor 1alpha (HIF1α) mutant on survival rate of transplanted fat particles through transfection of adipose-derived mesenchymal stem cells. METHODS:Recombinant adenovirus-mediated triple-mutant HIF1α was inserted into an adenovirus pAdEasy-1 system, followed by viral packaging and titer determination. Human adipose-derived mesenchymal stem cells were cultured, passaged and identified, and subsequently transfected with three kinds of viruses and blank vector (experimental group with transfection of the triple mutant of HIF1α; positive control group; negative control group; blank control group). Transfection efficiency was determined using enhanced green fluorescent protein labeling. Additionally, MTT assay was used to detect cell proliferation. RESULTS AND CONCLUSION:The recombinant adenovirus was successfully constructed and packaged in line with transfection requirements. Moreover, adipose-derived mesenchymal stem cells were successfully identified by adipogenic, osteogenic and chondrogenic induction and could be used as seed cells for subsequent experiments. RT-PCR results showed that HIF1α mRNA expression in the experimental group and positive control group was significantly higher than that in the other two groups(P < 0.05). Western blot analysis showed that the relative absorbance value in the experimental group was significantly higher than that in the other three groups (P < 0.05). A significant increase in the cell proliferation was found in the experimental group, significantly different from the other three groups (P < 0.05). Therefore, our findings indicate that transfection of adenovirus-mediated triple-mutant HIF1α not only can sustain the expression of target protein in transfected adipose-derived mesenchymal stem cells under normoxic conditions, but also can promote the proliferation of transfected adipose-derived mesenchymal stem cells.