慷舒灵治疗糖尿病足及下肢慢性创面疗效观察

目的探讨慷舒灵敷料治疗糖尿病足及下肢慢性创面的疗效。方法选择常规治疗四周以上无效的糖尿病足及下肢慢性创面患者16例，使用慷舒灵敷料治疗，视创面渗出的情况每3～5d换药1次，1周为一观察周期。结果5周愈合1例、6周愈合5例、7周愈合6例、10周愈合1例，11周愈合2例，有1例于12周行需要行自体皮片移植后创面愈合。慷舒灵全组总有效率为100％，8周愈合率为75％。结论慷舒灵敷料可有效治疗糖尿病足及下肢慢性创面，提高慢性创面愈合率，并可减少患者创面处理的痛苦。     

Biomimetic delivery of keratinocyte growth factor upon cellular demand for accelerated wound healing in vitro and in vivo

Exogenous keratinocyte growth factor (KGF) significantly enhances wound healing, but its use is hampered by a short biological half-life and lack of tissue selectivity. We used a biomimetic approach to achieve cell-controlled delivery of KGF by covalently attaching a fluorescent matrix-binding peptide that contained two domains: one recognized by factor XIII and the other by plasmin. Modified KGF was incorporated into the fibrin matrix at high concentration in a factor XIII-dependent manner. Cell-mediated activation of plasminogen to plasmin degraded the fibrin matrix and cleaved the peptides, releasing active KGF to the local microenvironment and enhancing epithelial cell proliferation and migration. To demonstrate in vivo effectiveness, we used a hybrid model of wound healing that involved transplanting human bioengineered skin onto athymic mice. At 6 weeks after grafting, the transplanted tissues underwent full thickness wounding and treatment with fibrin gels containing bound KGF. In contrast to topical KGF, fibrin-bound KGF persisted in the wounds for several days and was released gradually, resulting in significantly enhanced wound closure. A fibrinolytic inhibitor prevented this healing, indicating the requirement for cell-mediated fibrin degradation to release KGF. In conclusion, this biomimetic approach of localized, cell-controlled delivery of growth factors may accelerate healing of large full-thickness wounds and chronic wounds that are notoriously difficult to heal.     

A reliable method of determining wound healing rate

Several wound healing rate measures have been introduced with the main goal of enabling quantification of the effects of various therapeutic modalities on the healing of open wounds. Different definitions of wound healing rate render comparison of clinical results difficult. The goal of the present study was to propose a measure of wound healing rate that is independent of initial wound extent and to present a method of wound healing rate prediction. Comparisons were made of wound healing rate defined as absolute area healed per day, percentage of initial area healed per day and advance of the wound margin towards the wound centre per day. Analysis was performed on 300 wound cases. A disadvantage of wound healing measures that either use absolute area healed per day or percentage of initial area healed per day is their very limited use for comparing healing rates of wounds with different initial sizes. This disadvantage was overcome by incorporating a wound perimeter; thus obtaining a measure of the advance of the wound margin towards the wound centre. A definition of healing rate expressed as the greatest average wound margin distance from the wound centre divided by the time to complete wound closure is proposed. Because not all wounds are closed in the observation period, the time to complete wound closure has to be predicted. A method of wound healing rate prediction is presented based on a delayed exponential model the parameters of which are obtained from at least five weekly wound area measurements. Paired t-tests between actual time needed to complete wound closure and the predicted time resulted in p=0.062 after four, 0.484 after five and 0.900 after six weeks of observation.     

In vitro and in vivo assays on egg white/polyvinyl alcohol/clay nanocomposite hydrogel wound dressings

Novel nanocomposite hydrogel wound dressings on the basis of egg white and polyvinyl alcohol, as matrix, and natural Na-montmorillonite clay, as reinforcing agent, were prepared and their performances on wound healing investigated in vitro and in vivo. In vitro cytotoxicity assay revealed non-cytotoxic activity and excellent biocompatibility level of prepared nanocomposite hydrogel wound dressings. The bacterial penetration assay showed the prepared nanocomposite hydrogel wound dressings are excellent barriers against microorganisms and could protect the wound from infection during the wound healing. In vivo animal study showed that the wound healing process was considerably faster in wounds covered with nanocomposite hydrogel wound dressings compared to the conventional wound dressing, i.e. sterile gauze, due to creation of a moist environment on the wound surface and faster migration rate of the epidermal cells. The mechanical properties of healed wounds with nanocomposite hydrogel wound dressings were better than those control wounds covered with sterile gauze due to their better collagen formation ability as a result of created moist healing condition as well as the presence of egg white, as a source of proteins, in their structures.     

Transforming growth factor-beta3-loaded microtextured membranes for skin regeneration in dermal wounds

Adverse effects of wound healing, such as excessive scar tissue formation, wound contraction, or nonhealing wounds represent a major clinical issue in today's healthcare. Transforming growth factor (TGF)-beta3 has specifically been implicated in wound healing. Our hypothesis was that local administration of TGF-beta3 to excisional dermal wounds would diminish wound contraction and scar formation. Microtextured wound covers, containing different concentrations of TGF-beta3, were placed onto full-thickness excisional skin wounds in guinea pigs. Tattooed reference marks were used to quantify wound contraction. Sixty-four male guinea pigs in four study groups (5 ng TGF-beta3, 50 ng TGF-beta3, no growth factor, sham wound) were followed for up to 6 weeks. We analyzed 19 different parameters of wound healing. Results showed that, in some instances, the 50-ng TGF-beta3 group gave less contraction, whereas the 5-ng TGF-beta3 group gave more contraction. These differences confirm that TGF-beta3 has an optimum working concentration, and suggest this concentration to be closer to 50 ng than to 5 ng TGF-beta3. However, only very few significant differences occurred, and thus we conclude that the clinical relevance of our findings is negligible. Earlier studies, reporting clinically improved wound healing by TGF-beta3, could therefore not be confirmed by this study.     

Synergistic effect of bismuth subgallate and borneol,the major components of Sulbogin,on the healing of skin wound

Most skin lesions heal delay and even heal efficiently within 1-2 weeks, the healed tissue is neither aesthetically nor functionally perfect. Therefore, facilitating skin healing rate and controlling healed skin quality are major aims of drug treatment for a wound event. Bismuth subgallate (BS) and Borneol (BO) are the two components of Sulbogin, a new Vaseline-based wound healing ointment, one for treating skin wounds. Although BO has antibiotic function, while BS is widely used clinically, neither has been used specifically for wound healing.The experiment described here aimed to study the effect of BS and BO on the healing of skin wounds. This study also compared the effects of BS and BO with Flamazine cream, which is currently the most popular drug for wound healing in hospitals. Full-thickness wounds (3 cm x 3 cm x 0.2 cm) were created on the back of adult male Sprague-Dawley rats. BS, BO, BS+BO, and Flamazine were then evenly applied to cheesecloth and placed over the lesion areas. The drug patches were replaced every 2-3 days until the wound areas were completely covered by epidermis in any kinds of drug treatment. The combined BS and BO treatment had the best effect on healing by decreasing lesion area, while increasing granulation tissue formation, re-epithelialization, eating behavior and reconstitution of skin appendages. This investigation showed that BS and BO have a synergistic effect on the skin wound restoration.     

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models

We determined whether a two-part space-conforming polyethylene glycol/dopa polymer-based gel promoted healing of contaminated wounds in mice. This silver-catalysed gel was previously developed to be broadly microbiocidal in vitro while being biocompatible with human wound cell functioning. Full-thickness wounds were created on the backs of mice. The wounds were inoculated with 10(4) CFU of each of four common skin wound contaminants, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumanii and Clostridium perfringens. The wounds were then treated with our multifunctional polymer-based gel, the commercially available NewSkin product, or left to heal untreated. The untreated wounds were overtly infected, and presented detectable bacterial loads over the entire 21-day healing period, while the gel and NewSkin groups presented significantly smaller rises in bacterial levels and were cleared of detectable colonies by the third week, with the gel group clearing the bacteria earlier. While all three groups healed their wounds, the polymer-based gel-treated group demonstrated significantly earlier re-epithelialization and dermal maturation (P<0.05). This was reflected in a quick regain of tensile strength. This accelerated dermal maturation and regain in strength was noted in mice treated with the polymer-based gel when compared to wound treated with the commercially available Aquacel-Ag dressing (P<0.05). What distinguishes the polymer-based gel from these other products is that it is incorporated within the healing wound. These preclinical studies show that the anti-microbial polymer gel not only supports but also accelerates healing of bacterially contaminated wounds.     

In Vivo and Cytotoxic Assays of a Poly(vinyl alcohol)/Clay Nanocomposite Hydrogel Wound Dressing

In the present work, a nanocomposite hydrogel wound dressing was prepared on the basis of poly(vinyl alcohol) using organically-modified montmorillonite as nanoclay by the freezing–thawing cyclic method. In vivo assays were performed to evaluate its performance as an applicable wound dressing on animals. It showed an improved healing process for wounds covered by the prepared nanocomposite hydrogel compared with control wounds covered by sterile gauze. Significant improvements, such as better creation of moist surfaces on the wound with less scar formation, shorter duration of healing operation and better closing of the wound edges with enhanced tensile properties of the healed wound, i.e., tensile strength and elongation-at-break, were observed using the prepared nanocomposite hydrogel in comparison to the sterile gauze. An in vitro cytotoxic assay was also utilized to determine the biocompatibility of the prepared nanocomposite hydrogel. It showed that the prepared nanocomposite hydrogel is non-toxic and can be used as a biocompatible wound dressing in practical wound management.     

Development of a Wound Dressing Composed of Hyaluronic Acid Sponge Containing Arginine and Epidermal Growth Factor

Hyaluronic acid (HA) has the ability to promote wound healing. Epidermal growth factor (EGF) is able to promote the proliferation of various cell types, in addition to epidermal cells. A novel wound dressing was designed using high-molecular-weight hyaluronic acid (HMW-HA) and low-molecular-weight hyaluronic acid (LMW-HA). Spongy sheets composed of cross-linked high-molecular-weight hyaluronic acid (c-HMW-HA) were prepared by freeze-drying an aqueous solution of HMW-HA containing a crosslinking agent. Each spongy sheet was immersed into an aqueous solution of LMW-HA containing arginine (Arg) alone or both Arg and epidermal growth factor (EGF), and were then freeze-dried to prepare two types of product. One was a wound dressing composed of c-HMW-HA sponge containing LMW-HA and Arg (c-HMW-HA/LMW-HA + Arg; Group I). The other was a wound dressing composed of c-HMW-HA sponge containing LMW-HA, Arg and EGF (c-HMW-HA/LMW-HA + Arg + EGF; Group II). The efficacy of these products was evaluated in animal tests using rats. In the first experiment, each wound dressing was applied to a full-thickness skin defect with a diameter of 35 mm in the abdominal region of Sprague–Dawley (SD) rats, leaving an intact skin island measuring 15 mm in diameter in the central area of this skin defect. Commercially available polyurethane film dressing was then applied to each wound dressing as a covering material. In the control group, the wound surface was covered with polyurethane film dressing alone. Both wound dressings (Group I and Group II) potently decreased the size of the full-thickness skin defect and increased the size of the intact skin island, when compared with the control group. The wound dressing in Group II showed particularly potent activity in increasing the distance of epithelization from the intact skin island. This suggests that EGF release from the spongy sheet serves to promote epithelization. The wound dressing in Group II enhanced early-stage inflammation after 1 week, as compared with the other two groups. In the second experiment, each wound dressing was applied to a full-thickness skin defect measuring 35 mm in diameter in the abdominal region of SD rats, after removing necrotic skin caused by dermal burns. Polyurethane film dressing was applied to each wound dressing as a covering material. In the control group, the wound surface was covered with polyurethane film dressing alone. Both wound dressings (Group I and Group II) potently decreased the size of the full-thickness skin defect and increased epithelization from the wound margin, as compared with the control group. The wound dressing in Group II was found to enhance early-stage inflammation after 1 week, as compared with the other two groups. The findings in both experiments indicate that the wound dressing composed of HA-based spongy sheets containing Arg and EGF potently promotes wound healing by inducing moderate inflammation. The release of EGF in the early stages of wound healing induces moderate inflammation. This suggests that wound healing is facilitated directly by topical application of EGF, and indirectly by cytokines derived from inflammatory cells stimulated by EGF.     

Characterization of bacterial communities in venous insufficiency wounds by use of conventional culture and molecular diagnostic methods

Microbial infections delay wound healing, but the effect of the composition of the wound microbiome on healing parameters is unknown. To better understand bacterial communities in chronic wounds, we analyzed debridement samples from lower-extremity venous insufficiency ulcers using the following: conventional anaerobic and aerobic bacterial cultures; the Ibis T5000 universal biosensor (Abbott Molecular); and 16S 454 FLX titanium series pyrosequencing (Roche). Wound debridement samples were obtained from 10 patients monitored clinically for at least 6 months, at which point 5 of the 10 sampled wounds had healed. Pyrosequencing data revealed significantly higher bacterial abundance and diversity in wounds that had not healed at 6 months. Additionally, Actinomycetales was increased in wounds that had not healed, and Pseudomonadaceae was increased in wounds that had healed by the 6-month follow-up. Baseline wound surface area, duration, or analysis by Ibis or conventional culture did not reveal significant differences between wounds that healed after 6 months and those that did not. Thus, pyrosequencing identified distinctive baseline characteristics of wounds that did not heal by the 6-month follow-up, furthering our understanding of potentially unique microbiome characteristics of chronic wounds.     

创面放射性沾染的处置效果研究

目的探讨不同药物对创面放射性沾染的治疗与洗消效果。方法使用SD大鼠建立放射复合伤模型,建模后将SD大鼠随机均分为4组(对照组、复合中药膏组、纳米银组、负压引流敷料组)。对照组不敷药,创面用碘伏酒精擦拭后用纱布包扎;负压引流敷料组采用自适性负压引流敷料覆盖创面包扎,其余2组分别敷药后用纱布包扎。4组连续7 d换药,并分别在7、14、21 d取材,制作病理切片,观察创面愈合情况。结果建模后21 d,与对照组相比,其余3组所使用药物对创面愈合均有促进作用,差异有统计学意义(P<0.01),其中复合中药膏组愈合最快最好。除对照组外的3个组创口放射性均降低,差异有统计学意义(P<0.01),其中负压引流敷料组创口的放射性最小,说明负压引流敷料块对放射性沾染物的吸附作用高于纱布块的吸附作用。结论复合中药膏与负压引流敷料联合应用对于创面放射性沾染的处理既能更好地降低放射性,也能促进创口的愈合。     

磨削+生物敷料覆盖改善烧伤创面微循环的临床观察

目的磨痂+生物敷料覆盖面部烧伤创面后,改善了局部微循环,加速了创面愈合。方法面部深Ⅱ°创面采用普通钢丝球和电动磨痂机粗细兼顾,磨去创面坏死层,以基底泛红,有针尖状出血点为止,然后用生物敷料覆盖,创面愈合后一年内随访。结果本组病人48例,创面愈合时间7~12 d,平均10.5 d。45例肤色正常,皮肤弹性好,2例有花斑状色素沉着,1例有轻度的疤痕增生。结论磨痂+生物敷料覆盖可以改善局部的微循环,防止烧伤后微血栓的形成,阻断伤后进行性加重,能加速创面愈合。     

Development of a functional wound dressing composed of hyaluronic acid spongy sheet containing bioactive components: evaluation of wound healing potential in animal tests

This study aimed to develop a novel wound dressing composed of hyaluronic acid (HA) spongy sheet containing bioactive components. The wound dressing prepared by the freeze-drying method has a two-layered structure: an upper layer composed of cross-linked high-molecular-weight HA (HMW-HA) and a lower layer composed of low-molecular-weight HA (LMW-HA) containing arginine (Arg), magnesium ascorbyl phosphate (vitamin C derivative: VC), and epidermal growth factor (EGF) (referred to as EGF-dressing). A wound dressing containing only Arg and VC was prepared in a similar manner (referred to as EGF-free-dressing). The potential of each wound dressing was evaluated in animal tests using Sprague Dawley (SD) rats and diabetic mice. In the first experiment, each wound dressing was applied to a full-thickness skin defect in the abdominal region of SD rats. Wound conditions after 1?week and 2?weeks of treatment were evaluated based on macroscopic and histological appearance. A commercially available non-woven alginate wound dressing (Alg-dressing) was used in a control group. Both EGF-free-dressing and EGF-dressing decreased wound size and promoted granulation tissue formation associated with angiogenesis more effectively when compared with Alg-dressing. In particular, EGF-dressing promoted re-epithelialization. In the second experiment, each wound dressing was applied to a full-thickness skin defect in the dorsal region of diabetic mice. Wound conditions after 1?week and 2?weeks of treatment were evaluated based on macroscopic and histological appearance. A commercially available Alg-dressing was used in a control group. Both EGF-free-dressing and EGF-dressing decreased wound size and promoted granulation tissue formation associated with angiogenesis more effectively when compared with Alg-dressing. These findings indicate that EGF-free-dressing and EGF-dressing have the potential for more effective wound healing when compared with Alg-dressing. In particular, EGF-dressing has a higher potential for wound healing when compared with EGF-free-dressing.     

Differences in second-intention wound healing of distal aspect of the limb between Caspian miniature horses and donkeys: macroscopical aspects

In this study, second-intention healing of limb wounds was studied in donkeys and Caspian miniature horses. Full-thickness, rectangular (2?×?3?cm) skin wounds were created on the lateral aspect of left metacarpi of four donkeys and four Caspian horses. Wound healing process was compared between limb wounds of donkeys and Caspian horses by clinical and geometrical assessments, over a 4-week period. At days?0, 3, 7, 10, 14, 17, 21, 24, and 28, digital photographs were taken of all wounds. The area of epithelialization and granulation tissue was measured for each wound using sigma scan software. Percentages of wound contraction, epithelialization, and healing were calculated for each wound. The clinical and geometrical evaluation revealed that there were significant differences in second-intention wound healing patterns between donkeys and Caspian horses. The donkeys limb wound healed much faster and in better condition than the limb wound of the Caspian horses. Based on results of this study, it can be concluded that although Caspian miniature horses and donkeys have same body size, it seems that due to genetic differences, considerable heterogeneity was observed in second-intention wound healing between them.     

亲水性纤维含银敷料覆盖肛瘘术后创面的作用

背景：亲水性纤维含银敷料在其他创面治疗中目前已取得较多临床证据,但其 对肛瘘患者术后创面康复的作用研究较少。 目的：观察亲水性纤维含银敷料覆盖对肛瘘患者术后创面康复的作用。 方法：将57例肛瘘术后患者随机分为试验组29例和对照组28例,试验组在术后给予亲水性纤维含银敷料换药,3 d 1次,对照组术后给予无菌凡士林纱布换药,1次/d,观察两组患者的首次换药创面疼痛程度、渗液明显减少时间、换药次数、创面康复时间及换药费用等指标。 结果与结论：试验组首次换药时创面疼痛程度明显轻于对照组(P < 0.05),渗液明显减少时间、换药次数、创面康复时间明显少于对照组(P < 0.05);但试验组换药费用明显高于对照组(P < 0.05)。表明亲水性纤维含银敷料覆盖于肛瘘患者术后创面可减少刺激,明显减轻疼痛,提供湿性修复环境,加速创面愈合,减少换药次数,提高患者满意度。     

Effects of Zn-7® on open wound healing in dogs

Analysis of the accelerating effects of open wound healing by Zn-7 gel compared with normal saline was carried out in dogs. Five normal male, mixed breed dogs approximately 3 years old were selected. Histological and macroscopic aspects of second-intention wound healing were studied. Two rectangular, full-thickness skin wounds (20 × 30 mm) were created symmetrically on each dog at both sides of the dorsal midline at 0, 1, 2, 3, and 4 weeks. Left wounds were treated with Zn-7 gel (test group) and right wounds were treated with normal saline (control group) twice daily. Photographs were taken twice a week. Rulers were held vertically and horizontally close to the wound as a reference. The area of epithelialization and granulation tissue were measured for each wound, using the Scion Image software. Percentage wound contraction, epithelialization, and healing were calculated for each wound. No significant differences were observed in wound contraction, epithelialization and healing in the test and control groups (P > 0.05). At day 35 after initial wounding, biopsies were taken from wounds for histological examination. There were no significant differences in the number of inflammatory cells, fibrocyte, or amount of the fibrin and collagen (P > 0.05) between the test and control wounds.     

Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex

Smad3 mediates the intracellular signaling of TGF-beta1 superfamily and plays a critical role in the cellular proliferation, differentiation and elaboration of matrix pivotal to cutaneous wound healing. Smad3 antisense oligonucleotides (ASOs) impregnated polyelectrolyte complex (PEC) containing chitosan and sodium alginate was prepared for accelerated wound healing. Physicochemical properties of PEC were characterized by zeta potential, scanning electron microscopy and bioadhesive test. Full-thickness, excisional wounds were made on the dorsum of C57BL6 mice. Then, smad3 ASOs-PEC, PEC alone, smad3 ASOs and gauze dressing were applied to determine concentration of TGF-beta1 and collagen in tissues and observe the wound contraction and histology of tissues. Zeta potentials and bioadhesive strengths of ASOs-PEC were increased as the chitosan ratio in PEC. In smad3 ASOs-PEC, the healing process suggested by wound closure and histological observation was faster than other groups because collagen contents increased and level of TGF-beta1 decreased. These results demonstrate that the smad3 ASOs-PEC composed of chitosan and sodium alginate could be applied for accelerated wound healing.     

Modulation of the Keratinocyte–Fibroblast Paracrine Relationship with Gelatin-Based Semi-interpenetrating Networks Containing Bioactive Factors for Wound Repair

Gelatin-based semi-interpenetrating networks (sIPNs) containing soluble and covalently-linked bioactive factors have been shown to aid in wound healing; however, the biological responses elicited by the introduction of sIPN biomaterials remain unclear. In the current study, modulation of the re-epithelialization phase of wound healing by sIPNs grafted with PEGylated fibronectin-derived peptides and utilized as platforms for the delivery of exogenous keratinocyte growth factor (KGF) was evaluated. Following wounding, keratinocyte migration, proliferation and protein secretion is largely controlled by diffusible factors, such as KGF, released by the underlying fibroblasts. The impact of sIPNs and exogenous KGF upon the latter keratinocyte–fibroblast paracrine relationship and keratinocyte behavior was explored by monitoring keratinocyte adhesion and cytokine (IL-1α, IL-1β, IL-6, KGF, GM-CSF and TGF-α) release. Results were generally similar for keratinocyte monoculture and keratinocyte–fibroblast co-culture systems. Although keratinocyte adhesion increased over time for positive control surfaces, adhesion to the sIPNs remained low throughout the course of the study. Release of IL-1α and GM-CSF was increased by exogenous KGF. The effects were more noticeable on the positive control surfaces relative to the sIPN surfaces. Regulation of the release of TGF-α was surface dependent, while IL-6 release was dependent upon surface type, the inclusion of exogenous KGF and the presence of fibroblasts. The findings indicate that during re-epithelialization, sIPNs containing soluble bioactive factors aid in wound healing primarily by serving as conduits for KGF, which induces the release of other key cytokines involved in tissue repair.     

Homologous fibronectin enhances healing of excised wounds in rats.

In order to evaluate the effects of a topical application of homologous fibronectin on the healing of skin wounds, we made 2 excisional wounds on the back skin of each rat, applied ointment with or without fibronectin purified from citrated homologous plasma, and evaluated the effect according to wound size and microscopic findings. Excised lesions treated with carrier alone, but the difference was significant only in the early phase of wound healing, 2 and 3 days, according to wound size and microscopic changes. A significant decrease in wound size could be found in both groups, treated with ointment containing and not containing fibronectin, between day 4 and 9 when wound contraction was a major contributor to wound closure. Therefore it can be concluded that topical application of fibronectin has a beneficial effect on wound healing during its early phase, but no significant influence on wound contraction.     

微动力负压技术治疗小面积Ⅲ度低热烧伤创面的临床疗效观察

目的观察微动力负压技术(micro-dynamic negative pressure wound therapy, MDNPWT)应用于小面积Ⅲ度低热烧伤创面的临床疗效。 方法收集2016年1月至2018年5月于海军军医大学第一附属医院烧伤外科门诊就诊的小面积(≤10 cm²)Ⅲ度低热烧伤患者220例。应用MDNPWT治疗的108例患者设为微动力负压组，采用常规治疗的112例患者设为常规治疗组。微动力负压组患者入组后采取持续微动力负压护创敷料换药，而常规治疗组采用传统的外用药物治疗。对比微动力负压组和常规治疗组的创面愈合时间、换药次数、治疗总费用及手术率。对数据行t检验和χ²检验。 结果微动力负压组和常规治疗组的创面愈合时间分别为(31.8±3.5)、(48.0±13.9) d，2组比较差异有统计学意义(t＝11.75，P<0.05)；微动力负压组和常规治疗组的换药次数分别为(6.4±0.7)、(34.2±3.1)次，2组比较差异有统计学意义(t＝18.76，P<0.05)；微动力负压组和常规治疗组治疗总费用分别为(2 807.5±782.6)、(7 516.7±238.3)元，2组比较差异有统计学意义(t＝18.55，P<0.05)；微动力负压组和常规治疗组的手术率分别为0、70.5%，2组比较差异有统计学意义(χ²＝118.9，P<0.05)。 结论MDNPWT具有缩短创面愈合时间、减少换药次数、节省治疗总费用及降低小面积深度烧伤创面手术率等优点。因此，MDNPWT可以作为小面积Ⅲ度低热烧伤创面治疗安全有效的新方法。