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The effect of 1,25-dihydroxyvitamin D_3 [1,25(OH)_2D_3] and13-cis-retinoic acid(RA)on the proliferation of a novel human megakaryoblasticleukemia cell line(HIMeg)was investigated.At the concentration of 10~9 10~6mol/L,1,25(OH)_2D_3 and RA showed significant inhibition of the proliferation of themegakaryoblastic leukemic cells,which was demonstrated by the count of survival cells,incorporation of~3H-TdR and~3H-UR,and cloning efficiency in dose-dependent and time-dependent manners.The results can further explain the mechanism of differentiation-inducing agents and the effect of 1 ,25(OH)_2D_3 on myelofibrosis.It is possible for1,25(OH)_2D_3 and RA to be used to treat malignant megakaryocytic diseases. 相似文献
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作者观察了1,25-二羟维生素D_3[1,25(OH)_2D_3]的类似物MC903对人原始巨核白血病细胞系(HIMeg)生长分化的影响。结果显示,MC903可以明显地抑制HIMeg的生长并诱导其分化,MC903(10~(-10)～10~(-6)mol/L)几乎完全抑制了HIMeg细胞的克隆形成能力;经MC903作用4d后,细胞周期分布也发生明显改变,表现为G_0+G_1期细胞百分数明显增多,而S期及G_2+M期细胞百分数减少。MC903对HIMeg细胞生长分化的影响具有剂量和时间依赖性特点,且其作用效应强于1,25(OH)_2D_3。本文结果提示,MC903和1,25(OH)_2D_3对巨核细胞系的生长分化调节过程具有重要作用。 相似文献
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目的：研究腺苷酸环化酶激活剂ｆｏｒｓｋｏｌｉｎ（Ｆ）对１，２５－二羟维生素Ｄ３［１，２５（ＯＨ）２Ｄ３］受体（ＶＤＲ）ｍＲＮＡ表达的调节作用及其可能的生物学意义。方法：以人原始巨核白血病细胞系（ＨＩＭｅｇ）为模型，ＶＤＲｍＲＮＡ的检测采用定量逆转录－聚合酶链反应；细胞增殖实验采用悬浮培养系统及甲基纤维素培养系统。结果：Ｆ对ＨＩＭｅｇ细胞ＶＤＲｍＲＮＡ的表达具有向上调节作用，且有明显的时间依赖性特点；Ｆ本身虽然对ＨＩＭｅｇ细胞的增殖及分化过程无明显影响，但却可以显著地加强１，２５（ＯＨ）２Ｄ３对该细胞系的作用，具体表现在：当Ｆ同时存在时，１，２５（ＯＨ）２Ｄ３对ＨＩＭｅｇ细胞的增殖过程和克隆形成率的抑制作用更为明显，对ＨＩＭｅｇ细胞分化过程的诱导作用亦更为显著；同时，１，２５（ＯＨ）２Ｄ３对ＨＩＭｅｇ细胞周期分布的影响也更加突出。结论：Ｆ对ＨＩＭｅｇ细胞ＶＤＲｍＲＮＡ具有向上调节作用，并伴有１，２５（ＯＨ）２Ｄ３生物学效应的提高，说明下对ＨＩＭｅｇ细胞ＶＤＲｍＲＮＡ的向上调节作用具有生物学意义。 相似文献
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Objective To study the effect of 1,25-dihydroxyvitamin D[3] (1,25(OH)2D3) on the growth and apoptosis of breast cancer cell line MCF-7.
Methods Cell number was determined using the MTT method. Flow cytometric analysis was performed on cell cycles, and the percentage of apoptosis was counted. Apoptotic cells were quantified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and bcl-2 protein expression was estimated with Western blotting.
Results After incubation with 1,25(OH)2D3 10-7mol/L for 48 hours, MCF-7 cells exhibited significant growth in a dose- and time-dependent manner. Flow cytometric analysis indicated that cell numbers in GO/G1increased along with increasing apoptotic peak and percentage. With microscope and electron microscope observation, characteristics of apoptosis such as typical apoptotic bodies were commonly found. TUNEL also showed that 1,25(OH)2D3 10-8mol/Land 10-7mol/L groups had significantly high apoptosis percentage than control group with dose-dependence on induction apoptosis. And Western blot showed that 1,25(OH)2D3 10-8mol/L could down-regulate bcl-2 protein and 10-7mol/L could almost block bcl-2 protein expression.
Conclusions 1,25(OH)2D3 can inhibit cell growth with GO/G1 arrest, enhance the proliferation inhibition action of adriamycin, and induce apoptosis which may result from the down-regulation of the anti-apoptotic bcl-2 protein. 相似文献
Methods Cell number was determined using the MTT method. Flow cytometric analysis was performed on cell cycles, and the percentage of apoptosis was counted. Apoptotic cells were quantified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and bcl-2 protein expression was estimated with Western blotting.
Results After incubation with 1,25(OH)2D3 10-7mol/L for 48 hours, MCF-7 cells exhibited significant growth in a dose- and time-dependent manner. Flow cytometric analysis indicated that cell numbers in GO/G1increased along with increasing apoptotic peak and percentage. With microscope and electron microscope observation, characteristics of apoptosis such as typical apoptotic bodies were commonly found. TUNEL also showed that 1,25(OH)2D3 10-8mol/Land 10-7mol/L groups had significantly high apoptosis percentage than control group with dose-dependence on induction apoptosis. And Western blot showed that 1,25(OH)2D3 10-8mol/L could down-regulate bcl-2 protein and 10-7mol/L could almost block bcl-2 protein expression.
Conclusions 1,25(OH)2D3 can inhibit cell growth with GO/G1 arrest, enhance the proliferation inhibition action of adriamycin, and induce apoptosis which may result from the down-regulation of the anti-apoptotic bcl-2 protein. 相似文献
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1，25（OH）_2D_3及其类似物调节人原始巨核白血病细胞系增殖分化的机制 总被引：2，自引：1，他引：1
１，２５（ＯＨ）_２Ｄ_３及其两种新型类似物（ＭＣ９０３和ＥＢ１０８９）对人原始巨核白血病细胞系ＨＩＭｅｇ的增殖分化过程有重要调节作用。本研究利用分子杂交及逆转录－聚合酶链反应首次证明，ＨＩＭｅｇ细胞中有１，２５（ＯＨ）_２Ｄ_３受体（ＶＤＲ）ｍＲＮＡ的表达，强烈提示１，２５（ＯＨ）_２Ｄ_３对ＨＩＭｅｇ细胞的作用是由ＶＤＲ介导的。同时还发现，１，２５（ＯＨ）_２Ｄ_３及其类似物、维甲酸在调节ＨＩＭｅｇ细胞增殖分化过程中，Ｃ－ｍｙｃｍＲＮＡ表达水平迅速降低，６ｈ时已基本达到最低水平，提示这些作用因子对ＨＩＭｅｇ细胞的抑制增殖和诱导分化作用是和Ｃ－ｍｙｃ癌基因的表达改变密切相关的。 相似文献
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目的:探讨1,25-二羟维生素D3对人胃腺癌细胞增殖和细胞周期的影响。方法:1,25-二羟维生素D3作用于MGC-803细胞后,应用四甲基偶氮唑蓝(MTT)法测定细胞增殖能力、平板克隆试验测定细胞集落形成率、流式细胞仪测定细胞周期。结果:1,25-二羟维生素D3作用后胃腺癌细胞生长受抑制,细胞集落形成率明显下降(P<0.05);处理48 h后细胞周期出现向G0/G1期移行的特征性动力学改变。结论:1,25-二羟维生素D3对人胃腺癌细胞有抑制增殖、诱导分化作用。 相似文献
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目的研究1,25-二羟维生素D3[1,25(OH)2D3]对胃癌SGC-7901细胞增殖和细胞周期的影响,以及对细胞因子TNF-αmRNA及蛋白表达的影响。方法用不同浓度1,25(OH)2D3处理胃癌SGC-7901细胞,采用MTT法检测细胞增殖情况;流式细胞仪检测细胞生长周期;RT-PCR和Western blotting分别检测TNF-α在mRNA和蛋白水平的表达情况。结果 1,25(OH)2D3对胃癌SGC-7901细胞增殖有抑制作用(P<0.05),并呈时间剂量依赖性。经1,25(OH)2D3处理72 h后细胞周期出现向G0/G1期移行的动力学改变(F=9.81,P<0.05)。TNF-αmRNA及蛋白的表达水平均呈剂量依赖性减少(P<0.05)。结论 1,25(OH)2D3可抑制胃癌细胞增殖,且与其抑制TNF-α的表达可能有相关性。 相似文献
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Ｍｏｌｅｃｕｌａｒｍｅｃｈａｎｉｓｍｏｆｔｈｅｅｆｆｅｃｔｓｏｆ１，２５－ｄｉｈｙｄｒｏｘｙｖｉｔａｍｉｎｎＤ_３ａｎｄｉｔｓｎｏｖｅｌａｎａｌｏｇｕｅｓｏｎｐｒｏｌｉｆｅｒａｔｉｏｎａｎｄｄｉｆｆｅｒｅｎｔｉａｔｉｏｎｏｆａｈｕｍａｎｍｅｇａｋａｒ?.. 相似文献
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1,25双羟维生素D3及转化生长因子β1对人胚成骨细胞增殖和分化的影响 总被引：1，自引：0，他引：1
目的 研究 1,2 5双羟维生素D3 [1,2 5 (OH) 2 VD3 ]及人转化生长因子 β1(hTGF β1)对人胚成骨细胞增殖和分化的影响。方法 体外培养人胚成骨细胞 ,经 1,2 5 (OH) 2 VD3 及hTGF β1作用后 ,通过四唑盐 (MTT)比色法观察细胞增殖 ;检测细胞培养液中碱性磷酸酶 (ALP)活性及骨钙素 (OC)含量 ;逆转录 聚合酶链反应 (RT PCR)方法观察细胞中TGF β信号传导关键蛋白 SMAD蛋白mRNA水平的变化。结果 MTT比色发现 1,2 5 (OH) 2 VD3 组的吸光度值 (OD值 )较对照组下降 30 6 % (0 0 86±0 0 2 2比 0 12 4± 0 0 31,P <0 0 5 )。细胞培养液中ALP活性在 1,2 5 (OH) 2 VD3 组、hTGF β1组以及二者联合应用组均有显著增高 ,为对照组的 1 3～ 2 0倍 (P <0 0 5 )。其中 ,当hTGF β1浓度为 1× 10 -6g/L时 ,1,2 5 (OH) 2 VD3 及hTGF β1对人胚成骨细胞ALP的分泌存在协同刺激作用。 1,2 5 (OH) 2 VD3 与 1×10 -6g/L和 1× 10 -5g/L浓度的hTGF β1合用使得细胞培养液中OC水平增加 (P <0 0 5 ) ,但未发现二者的协同作用。当 1,2 5 (OH) 2 VD3 与 1× 10 -6g/L的hTGF β1合用时SMAD3mRNA水平达高峰 ,约为对照组的 6倍。结论 1,2 5 (OH) 2 VD3 抑制人胚成骨细胞增殖 ,促进其分化。 1,2 5 (OH) 2 VD3 及hTGF β1 相似文献