HPCE测定不同产地夏枯草中齐墩果酸、熊果酸、迷迭香酸含量

目的:建立高效毛细管电泳法测定夏枯草中齐墩果酸、熊果酸、迷迭香酸含量的方法.方法:采用胶束毛细管电动法测定齐墩果酸、熊果酸的含量,毛细管区带电泳法测定迷迭香酸的含量.结果:齐墩果酸、熊果酸、迷迭香酸分别在0.054 8 -0.657 6 g/L,0.102 4 -0.819 2 g/L,0.0204-0.163 2 g...     

葛根芩连方药中小檗碱、药根碱和巴马汀的高效毛细管电泳分析

目的 以高效毛细管电泳法分离并测定葛根芩连方药中黄连生物碱。方法 通过毛细管区带电泳法，以60％的磷酸盐(60mmol／L，pH=8．0)与40％甲醇为缓冲液，苄基三乙基氯化铵为内标，紫外254nm检测，分离并测定葛根芩连汤水提物及微丸中小檗碱、巴马汀、药根碱的含量。结果 黄连中生物碱在6min内得到很好的分离，小檗碱、巴马汀、药根碱的加样回收率分别为99．05％、98．70％和97．76％；相对标准偏差(RSD)分别为2．12％、1．85％和1．95％。结论 该方法具有良好的精密席和回收率，可作为黄连制剂的研究提供含量测定方法。     

高效毛细管电泳法测定尿液中柠檬酸盐、草酸盐的含量

目的 以高效毛细管电泳法测定尿液中柠檬酸盐、草酸盐的含量。方法 以毛细管区带电泳分离分析尿样中的柠檬酸盐、草酸盐，以铬酸钠为紫外吸收剂进行间接紫外检测。结果 柠檬酸根、草酸根阴离子在5min内得到很好的分离，迁移时间分别为3．76、3．14min，柠檬酸根在4．00、40．00μg／m1浓度范围内、草酸根在2．00-40．00μg／ml浓度范围内线性关系良好，柠檬酸根阴离子最低检测限为2μg／ml、草酸根阴离子的最低检测限为1μg／ml。加样回收率分别为97．53％、99．11％。结论 该方法简单．准确．成本低．可为尿液中柠檬酸盐、草酸盐的直接测定提供参考。     

喂饲补阳还五汤的大鼠血清中阿魏酸的高效毛细管电泳分析

目的　采用高效毛细管电泳法测定补阳还五汤及给药大鼠血清中阿魏酸的含量。方法　以毛细管区带电泳分离分析待测样品中的阿魏酸 ,内标法定量 ,紫外检测 ,缓冲液为 2 5 m mol/ L 硼砂 -甲醇 (85∶ 15 ) ,未涂层融硅毛细管 (5 2 cm× 5 0μm,有效长度 30 cm ) ,分离电压 2 0 k V,压力进样 3s,检测波长 32 0 nm ,温度 30℃。结果　在上述条件下 ,阿魏酸在 6 min内得到很好的分离 ,血清中阿魏酸的加样回收率为 97.2 0 % ,补阳还五汤中阿魏酸的回收率为 10 3.2 8% ,RSD分别为 3.73%和 0 .91% (n=3)。结论　该方法可为血清样品及复方制剂中阿魏酸的含量测定提供参考。     

高效液相层析法检查β地中海贫血应用评价

目的 对高效液相层析法快速筛查β地中海贫血进行评价。方法 用高效液相层析法测定血中血红蛋白A2（HbA2）、血红蛋白F（HbF）的含量，并与抗碱变试验法测得HbF、琼脂糖电泳法测得HbA2的含量进行比较；取低、中、高三种浓度值的标本进行重复性试验；在HbF含量为3．5％、5．7％和HbA2含量为5．9％、7．7％的两份标本中加入不同浓度的胆红素液及脂肪乳作干扰试验；与已知HbF浓度为33．0％和1．1％的两份标本按不同比例混合，分别进行测定，观察该法测定的线性范围。结果 该法测定HbF与抗碱变试验法结果相符、HbA2与琼脂糖电泳法有良好相关（r=0．8656）；HbF的线性范围可达33％；HbF和HbA2批内变异系数分别为1．5％～3．4％和3．7％～5．9％，批间变异系数分别为1．9％～4．2％和4．9％～7．5％；胆红素浓度在250μmol／L，甘油三酯浓度在22mmol／L以下时对测定结果无影响。结论 高效液相层析法测定HbF和HbA2具有准确度高，重复性好，不受脂浊、黄胆标本的干扰，且检测速度快，对G地中海贫血具有较好的初筛作用。     

高效毛细管电泳法测定桑叶中芦丁含量

目的：探讨高效毛细管电泳法（HPCE）在测定桑叶中芦丁含量的作用。方法：选择新疆不同采集期桑叶。HPCE条件：熔融石英毛细管50cm×50μm，uncoated；硼砂和磷酸二氢钠缓冲溶液20mmol／L，其中含15％甲醇溶液，pH8．5；分离电压20kV（＋）-（-）；电泳时间12min；电泳温度25℃；压力进样0．5psi×10s；柱上检测UV254nm（二极管阵列检测器）。结果：样品可在10min内出峰，测得蒜氨酸浓度在O～80μg／ml范围内与峰面积的线性关系良好（r=0．9945），平均回收率为98．9％，批内RSD为3．9％。结论：高效毛细管电泳法准确、简单、快捷、重现性好。     

5%声振人血白蛋白空气微球蛋白含量测定

采用高效液相凝胶色谱汉（ＧＣ－ＨＰＬＣ）、醋酸纤维膜电泳和半微量定氮法测定５％声振人血白蛋白空气微球制剂中球壁蛋白的含量。ＧＣ－ＨＰＬＣ确证了球壁蛋白主要成分为人血白蛋白；醋酸纤维膜电泳测定空气人血白蛋微球制剂中人血白蛋白纯度＞９０％；半微量定氮法测出球壁蛋白含量平均值为３．５６％左右，９９％正常值范围为２．５２％～４．６０％。     

高效毛细管电泳法测定血清中头孢他啶的含量

采用高效毛细管电泳法测定血清中头孢他啶的含量。毛细管 6 0cm× 75 μm ;运行缓冲液 2 5mmol/L四硼酸钠 (pH8 2 ) ,高压进样 5s,分离电压 2 0kV ,温度为 2 5℃ ,检测波长为 2 5 4nm ,地塞米松磷酸钠为内标。头孢他啶在 0 .6 2 5～ 2 0 μg/mL浓度范围内线性关系良好 (r =0 .9997) ,平均回收率为 98.81 % (n =5 ,RSD =1 .88% )。     

毛细管区带电泳法测定牡丹皮中芍药苷的含量

牡丹皮为毛茛科植物牡丹 Paeonia suffruticosaAndr.的干燥根皮 ,具有清热凉血、活血化瘀之功效 ,鲜根皮含芍药苷 ( paeoniflorin)、羟基芍药苷、苯甲酰芍药苷、丹皮酚苷、丹皮酚等。芍药苷是牡丹皮中主要活性成分之一 ,具有镇痛、镇痉、镇静、抗炎等作用。目前 ,牡丹皮中芍药苷的含量测定方法主要有高效液相色谱法[1] 和薄层扫描法[2 ,3] ,毛细管电泳法测定牡丹皮中芍药苷的含量尚未见报道。本研究用毛细管区带电泳法测定了牡丹皮中芍药苷的含量 ,方法简便快捷 ,结果令人满意 ,为牡丹皮的质量控制提供了一种新方法。1　仪器与药品毛细管电…     

高效毛细管电泳法测定泰特中还原型和氧化型谷胱甘肽

用高效毛细管电泳法测定泰特中还原型和氧化型谷光甘肽含量。电泳系统由１００ｍｍｏｌ／Ｌ硼酸盐缓冲液和１０ｍｍｏｌ／ＬＮ－［２－羟基－１，１－二羟甲基－乙基］甘氨酸（ｔｒｉｃｉｎｅ）组成（ｐＨ８．５）；工作电压２０ｋＶ，检测波长２００ｎｍ，６ｍｉｎ内两组分达到基线分离。选用脲苷作内标物进行测定，两组分在２０～５００μｇ／ｍｌ范围内呈线性。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.