Percutaneous absorption of [7.10-14C]benzo[a]pyrene and [7,12-14C]dimethylbenz[a]anthracene in mice

The percutaneous penetration, tissue distribution, and excretion of 14C-labeled benzo[a]pyrene (BaP) and dimethylbenz[a]anthracene (DMBA) were studied in mice. Both BaP and DMBA rapidly penetrated the skin and were excreted more in the feces than in the urine. The proportion of BaP or DMBA absorbed was less with increasing applied dose due to apparent saturation of the uptake process. Uptake from the dorsal skin of the nose was similar to uptake from the dorsal nuchal skin.     

苯并[a]芘在大鼠脑组织中分布动态观察

目的 观察[¹⁴C]标记苯并[a]芘在大鼠脑内的分布。方法 将100只SD大鼠随机分为对照组和实验组,实验组尾静脉注射[¹⁴C]标记苯并[a]芘3.7×10⁵Bq/kg,对照组注射等量生理盐水;在注射后1,6,12,24和48 h用光镜放射自显影法观察苯并[a]芘在脑内的分布情况。结果 与对照组比较,实验组大鼠脑组织注药后1 h即有苯并[a]芘银粒出现,随着时间的增加,银粒数也逐渐增加,24 h达高峰,48 h明显减少,注射后1,6,12,24,48 h银粒数分别为(17.68±1.79),(22.67±2.15),(25.79±2.55),(32.33±2.78),(18.01±1.68)粒,差异有统计学意义(F=69.67,P<0.01);银粒在脑中分布不均匀,注药后1 h主要集中在海马,6 h主要集中于大脑皮层,12 h则分布于纹状体;各时相脑组织中神经元银粒数明显高于神经胶质细胞(P<0.05)。结论 苯并[a]芘在各脑区的分布不均匀,24 h达到高峰,神经元细胞可能是苯并[a]芘靶细胞。     

Anguilla anguilla L. biochemical and genotoxic responses to benzo[a]pyrene

Eels (Anguilla anguilla L.) were exposed for 2, 4, 6, 8, 16, 24, 48, 72, 144, and 216 h to 0 (control), 0.3, 0.9, and 2.7 microM benzo[a]pyrene (BaP). The biotransformation induced by BaP was measured as liver ethoxyresorufin O-deethylase (EROD) activity and cytochrome P450 content, and compared with the genotoxic effects, such as erythrocytic nuclear abnormalities (ENA), and blood and liver DNA strand breaks. The liver exhibited a highly significant EROD activity increase from 2 up to 216 h exposure to 0.9 and 2.7 microM BaP, whereas 0.3 microM BaP exposure induced a significant liver EROD increase from 2 up to 144 h. Liver cytochrome P-450 content was significantly increased at 8 h to 2.7 microM BaP exposure. Liver DNA integrity was decreased at 16 h, from 8 up to 144 h and 8 up to 72 h exposure to 0.3, 0.9, and 2.7 microM BaP, respectively. A significant decrease in blood DNA integrity was observed at 48, 72, 144 h, from 8 up to 72, and from 6 up to 72 h exposure to 0.3, 0.9, and 2.7 microM BaP, respectively. The A. anguilla L. genotoxic response to BaP, measured as ENA induction, was significantly increased at 144 h exposure to 0.3 microM BaP. The intermediate BaP concentration tested (0.9 microM) induced a significant three fold ENA increase at 48 and 72 h exposure compared to their controls. The highest BaP concentration (2.7 microM) induced a significant increase in ENA frequency at 72, 144 and 216 h exposure.     

双壳类水产品中(+)-anti-BPDE-DNA加合物HPLC/FD检测

目的 建立检测双壳类水产品中（+）-反-7,8-二羟基-9,10-环氧苯并（a）芘（BPDE）-DNA加合物的高效液相色谱荧光法（HPLC/FD）。方法 用组织基因组DNA提取试剂盒提取6种双壳类水产品组织中的DNA,0.1 mol/L HCl 、90 ℃酸解4 h,乙酸乙酯充分萃取酸解产物——苯并（a）芘-四醇。以CenturySIL C18-BDS色谱柱（150 mm×4.6 mm,5 μm）分离;洗脱流动相：V（甲醇）/V（水）=55/45;流速：1.0 mL/min;进样量：20 μL;激发波长：265 nm,发射波长：395 nm;荧光检测苯并（a）芘-四醇的含量。结果 该方法的检测限为0.3 ng/mL,在0.5~100 ng/mL范围内呈良好的线性关系（r²=0.9960）;日内相对标准偏差（RSD）为2.8%~4.2%,日间RSD为3.2%~5.8%;双壳类水产品中（+）-anti-BPDE-DNA含量为13.44~152.7 μg/kg,RSD为3.0%~6.5%;加标回收率为86.9%~91.6%,RSD为3.1%~7.3%。结论 该法简便、快速、灵敏度高,可用于双壳类水产品中（+）-anti-BPDE-DNA加合物的检测。