渭良伤科散超微粉巴布膏的抗炎镇痛实验研究

目的探讨渭良伤科散超微粉巴布膏的抗炎和镇痛作用。方法通过热板法和冰醋酸致痛试验、角叉菜胶致大鼠足趾肿胀试验、二甲苯致小鼠耳廓肿胀试验,对渭良伤科散超微粉巴布膏进行镇痛抗炎方面的药效学评价。结果渭良伤科散超微粉巴布膏中、高剂量的剂型可有效提高小鼠痛阈值,减少小鼠扭体反应次数,抑制角叉菜胶所致的大鼠足趾肿胀和抑制二甲苯所致的小鼠耳廓肿胀。结论渭良伤科散超微粉巴布膏中、高剂量的剂型有较好的镇痛抗炎作用,且在方便使用的基础上,优于原剂型渭良伤科散的镇痛抗炎作用。     

小茴香茎叶水提液的抗炎镇痛作用研究

目的:评价小茴香水提液的抗炎、镇痛作用.方法:应用二甲苯致小鼠耳廓肿胀研究小茴香水提液的抗炎作用,采用热板法、甲醛法、醋酸致小鼠扭体反应研究小茴香水提液的镇痛作用.结果:小茴香水提液提取物各剂量组与模型组比较,能降低二甲苯所致小鼠的耳廓肿胀度(P＜0.05);对热痛刺激的反应时间得到延缓(P＜0.05);对甲醛所致的疼...     

温中安胃软胶囊药效学研究

目的:评价温中安胃软胶囊的主要药效.方法:采用热板法、扭体法观察镇痛作用,二甲苯致小鼠耳肿胀法及角叉菜胶致大鼠足趾肿胀法观察抗炎作用,小鼠大黄致泻法观察止泻作用,酚红排泄法观察对小鼠胃排空的影响,炭末推进法观察对小鼠肠推进的影响.结果:温中安胃软胶囊能提高小鼠热刺激痛阈,减少小鼠因冰醋酸致扭体反应数,对二甲苯致小鼠耳肿胀及角叉菜胶致大鼠足趾肿胀有抑制作用,减少大黄煎剂所致小鼠湿粪数,对正常及胃复安负荷小鼠胃排空有抑制作用,对阿托品负荷小鼠胃排空抑制亦有对抗作用,对正常小鼠及新斯的明负荷小鼠肠推进有抑制作用.结论:温中安胃软胶囊有抗炎、镇痛、止泻及调节胃肠运动作用.     

痛经宁胶囊对雌性幼鼠生殖器官发育的影响及其镇痛抗炎作用

目的:观察痛经宁胶囊(当归、川芎、香附等)对♀幼鼠生殖器官发育的影响及其镇痛抗炎作用.方法:对小鼠子宫、卵巢重量测定,记录小鼠扭体次数和热刺激痛阈,二甲苯致小鼠耳壳肿胀法,大鼠肉芽肿模型法及蛋清致大鼠足趾肿胀法.结果:痛经宁胶囊能明显增加♀幼鼠卵巢和子宫重量,减少醋酸所致小鼠扭体次数,延长小鼠热刺激痛阈,显著抑制二甲苯所致的小鼠耳壳肿胀度和肿胀百分率,显著抑制大鼠肉芽肿胀和蛋清所致大鼠足趾肿胀值及足趾肿胀百分率.结论:痛经宁胶囊能促进♀幼鼠生殖器官的发育,有镇痛抗炎作用.     

康妇炎胶囊抗炎镇痛的药效学研究

目的研究康妇炎胶囊的抗炎、镇痛作用。方法通过二甲苯致小鼠耳廓肿胀、醋酸致小鼠腹腔毛细血管通透性和琼脂致小鼠皮下肉芽肿实验观察康妇炎胶囊的抗炎作用,采用热板法和扭体法观察其镇痛作用。结果康妇炎胶囊能够显著抑制二甲苯所致小鼠耳廓肿胀,显著降低小鼠腹腔毛细血管通透性,抑制小鼠皮下肉芽肿的形成;并明显降低热刺激所致的小鼠痛阈值,减少小鼠的扭体次数。结论康妇炎胶囊对炎症有明显的抑制作用,且有一定的镇痛效果。     

大蒜素通过抗氧化应激缓解小鼠炎性痛的机制研究

目的探讨大蒜素抗炎性痛作用及抗氧化应激机制。方法采用冰醋酸致小鼠扭体、小鼠足底注射甲醛致痛、二甲苯致小鼠耳廓肿胀及角叉菜胶致小鼠足趾肿胀等实验,观察不同剂量大蒜素的抗炎性痛效应;采用生化法检测角叉菜胶致小鼠足趾肿胀组织SOD、MDA、GSH-Px及T-AOC含量变化。计量资料比较采用t检验,P<0.05为差异有统计学意义。结果与阴性对照组比较,大蒜素20 mg/kg组及40 mg/kg组小鼠扭体次数和累计舔足时间均降低;大蒜素40mg/kg组小鼠耳廓肿胀度和小鼠足趾肿胀度均低于阴性对照组,差异有统计学意义(均P<0.05)。与模型组比较,大蒜素40 mg/kg组小鼠足趾肿胀组织SOD、GSH-Px、T-AOC含量增加,差异有统计学意义(均P<0.05)。结论大蒜素对小鼠炎性痛有一定的缓解作用,其机制与抗氧化应激作用有关。     

消肿止痛液的药效学研究

目的研究消肿止痛液对实验动物炎性作用、镇痛作用以及活血化瘀作用的影响.方法分别以SD大鼠、小鼠为实验动物,观察消肿止痛液对角叉菜胶致大鼠足肿胀、二甲苯致小鼠耳廓炎症的作用,以及对镇痛、活血化瘀作用的影响.结果消肿止痛液局部涂擦能明显减轻角叉菜胶所致大鼠足肿胀和二甲苯致小鼠耳廓炎症：明显延长小鼠的痛阈值,减少小鼠扭体次数;显著促进小鼠瘀血吸收.结论消肿止痛液有消炎、镇痛和化瘀作用.     

荭草煎剂对小鼠抗炎镇痛作用初探

目的 探讨荭草的抗炎、镇痛作用.方法 采用二甲苯致小鼠耳廓肿胀,观察高、中、低剂量荭草水煎剂的抗炎作用;采用热板法致痛,观察高、中、低剂量荭草水煎剂的镇痛作用.结果 高、中、低剂量荭草水煎剂明显抑制二甲苯所致小鼠耳廓的肿胀度,与生理盐水对照组比较差异有统计学意义(P＜0.05),与赖氨匹林组比较差异无统计学意义(P＞0.05).高剂量荭草水煎剂显著提高热板法所致小鼠的痛阈值,与生理盐水对照组比较和自身给药前比较差异有统计学意义(P＜0.05);中剂量荭草水煎剂给药后30、60 min显著提高热板法所致小鼠的痛阈值,与生理盐水对照组比较和自身给药前比较差异有统计学意义(P＜0.05).结论 荭草水煎剂具有抗炎、镇痛作用.     

强脊壮步胶囊治疗腰椎间盘突出症的主要药效学与急性毒性实验研究

目的:对强脊壮步胶囊主要药效学与急性毒性进行实验研究。方法:以醋酸致小鼠扭体实验和热板刺激致小鼠舔后足实验为镇痛药效学指标,以二甲苯致小鼠耳廓肿胀实验和甲醛致小鼠足肿胀实验为抗炎药效学指标,研究强脊壮步胶囊的镇痛、抗炎作用;同时考察了强脊壮步胶囊的一日最大给药量。结果:强脊壮步胶囊具有一定的抗炎、镇痛作用,可明显延长疼痛潜伏期,降低耳廓与后足趾的肿胀度;小鼠灌胃的最大给药量为50g/kg,最大给药量是临床用药量的463倍。结论:强脊壮步胶囊具有一定的镇痛抗炎作用,急性毒性试验结果提示该药按临床剂量短期内使用安全。     

杭白菊挥发油的抗菌抗炎作用及对PGE2的影响

目的研究杭白菊挥发油抗菌抗炎作用及其可能的机制。方法采用体外抗菌法、小鼠耳壳二甲苯肿胀法、角叉菜胶致大鼠足趾肿胀法,观察不同浓度和剂量杭白菊挥发油对细菌和炎症的影响,并检测PGE2的含量。结果杭白菊挥发油有明显的抗菌效果;对二甲苯致小鼠耳壳肿胀、角叉菜胶致大鼠足肿胀有抑制作用,并能减少PGE2的含量。结论杭白菊挥发油有抗菌抗炎作用,其抗炎作用可能与抑制PGE2的产生有关。     

健儿清解液的药效学研究

目的通过动物实验研究健儿清解液(JQOL)的药效学作用。方法采用酵母致热、氨水引咳、二甲苯致耳肿胀、醋酸致腹膜通透性增高等模型,并测定小鼠最大耐受量。结果大鼠灌胃给予JQOL18.14、9.072、4.536g/kg可显著抑制酵母性发热时的体温升高;给予JQOL11.35、7.258、2.835g/kg可明显延长氨水引咳小鼠的咳嗽潜伏期,并能减少其咳嗽次数,能显著拮抗小鼠二甲苯性耳肿胀,降低腹膜通透性。小鼠最大耐受量为人用量的205.2倍。结论健儿清解液具有清热、止咳、抗炎作用,临床用量是安全的。     

泰山散瘀膏抗炎镇痛作用的实验研究

目的：研究泰山散瘀膏的抗炎镇痛作用.方法：取小鼠32 只,随机分为致炎液实验组及致炎液对照组、冰醋酸实验组及冰醋酸对照组,每组8 只,利用致炎液建小鼠耳廓肿胀模型,观察泰山散瘀膏的抗炎作用;采用小鼠醋酸扭体法研究泰山散瘀膏的镇痛作用.结果：泰山散瘀膏能显著降低小鼠耳廓肿胀程度,显著抑制冰醋酸所知疼痛后的扭体次数.结论：泰山散瘀膏具有明显的抗炎阵痛作用.     

Gossypol acetate-induced SCEs in spermatogonia and bone marrow cells of mice: dose-response relationships

Gossypol acetic acid has been shown to have the effect of increasing the SCE frequencies in the spermatogonial cells of mice. This study further confirmed the genotoxic effects of gossypol acetic acid by investigating the dose-response relationships of gossypol acetic acid-induced SCEs in spermatogonial cells and bone marrow cells of mice. Twelve or over 12 mg/kg body weight of the dosage per day could increase the SCE frequencies of both bone marrow cells and spermatogonial cells. The increase of SCE frequency appeared to be directly correlated with the doses used at significant levels (p less than 0.001). Regression lines indicated that, in spite of cell type variation, the SCE induced was proportional to the dose of gossypol acetic acid.     

An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols

Extracts obtained from the seeds of Nigella sativa are used as a spice or remedy for the treatment of various inflammatory diseases. The purpose of this study was to examine the analgesic and anti-inflammatory effects of its polyphenols. N. sativa seed polyphenols were prepared, and analgesic and anti-inflammatory effects were studied in mice and rats using the acetic acid-induced writhing, formalin, light tail flick, carrageenan-induced paw edema, and croton oil-induced ear edema tests. In the acetic acid-induced writhing test, oral administration of N. sativa polyphenols (NSP) decreased the number of abdominal constrictions. Both oral and intraperitoneal administration of NSP significantly suppressed in a dose-dependent manner the nociceptive response in the early and late phases of the formalin test, and the effect on the late phase was more pronounced. Pretreatment with naloxone failed to reverse the analgesic activity of NSP in this test. NSP did not produce a significant analgesia in the light tail flick test in mice. Oral administration of NSP did not produce a significant reduction in carrageenan-induced paw edema. However, when injected intraperitoneally, NSP inhibited paw edema in a dosedependent manner. NSP when applied topically failed to reduce croton oil-induced ear edema. These results suggest that NSP have analgesic and anti-inflammatory effects. The lack of analgesic effect of NSP in the light tail flick test and also the failure of naloxone to reverse the analgesia in the formalin test reveal that mechanisms other than stimulation of opioid receptors are involved.     

吡罗昔康新制剂微乳的药效学研究

目的：制备吡罗昔康微乳新剂型,对其抗炎、镇痛作用进行初步药效学研究,观察连续用药对皮肤组织形态学的改变,并与凝胶制剂进行比较。方法：采用油相Lauroglycol FCC,表面活性剂Labrasol、Cremophor EL,助表面活性剂TranscutolP、吡罗昔康及水制备吡罗昔康微乳,1％的双氯芬酸钠凝胶剂作为阳性对照药,采用二甲苯诱导小鼠耳廓肿胀,卡拉胶诱发大鼠足跖肿胀,痛阈提高率和醋酸致小鼠扭体实验,对吡罗昔康微乳的抗炎镇痛作用进行初步探讨并与市售的吡罗昔康凝胶进行比较。结果：吡罗昔康微乳具有抗炎镇痛作用,中、低剂量的吡罗昔康微乳与阳性对照组比较均显示相近或更强的抗炎镇痛作用,与吡罗昔康凝胶组比较,作用强、时间长、效果平稳,且对皮肤无刺激性。结论：吡罗昔康新制剂微乳安全,抗炎镇痛作用明显,有望成为吡罗昔康的新型制剂。     

醋酸舍莫瑞林的免疫活性研究

目的　观察醋酸舍莫瑞林对免疫抑制模型小鼠免疫功能的调节作用。方法　通过给小鼠腹腔注射免疫抑制剂环磷酰胺 ,建立免疫功能低下模型 ,腹腔注射醋酸舍莫瑞林进行体内拮抗实验。测定胸腺指数、脾脏指数、巨噬细胞吞噬功能、T淋巴细胞的增殖指数、耳肿胀度、血清IL - 2的含量。结果　醋酸舍莫瑞林 ( 0 18,0 36mg/kg)能显著提高被环磷酰胺抑制的胸腺和脾脏重量 (P <0 0 1) ;使巨噬细胞吞噬功能明显增加 ,并能拮抗环磷酰胺对小鼠脾脏T淋巴细胞增殖、二硝基氯苯 (DNCB)诱导的迟发型超敏反应和IL - 2产生的免疫抑制作用。结论　醋酸舍莫瑞林能拮抗环磷酰胺所引发的免疫抑制作用     

脱氧雪腐镰刀菌烯醇致幼鼠成年鼠骨及关节软骨损伤差异的实验研究

目的观察脱氧雪腐镰刀菌烯醇(DON)致Wistar幼鼠、成年鼠膝关节骨与软骨损伤的异同。方法 24只健康雄性Wistar幼鼠(20日龄)和24只健康雄性Wistar成鼠(60日龄),分别随机分为正常对照组、DON低剂量组、DON高剂量组,每组各8只,共6组;幼鼠和成鼠的低剂量组和高剂量组均通过灌胃的方式隔日染毒,低剂量灌胃剂量为0.06 mg/kg,高剂量灌胃剂量为0.25 mg/kg;对照组使用相同体积的生理盐水。作用80 d后,取双侧膝关节,进行光镜和电镜病理形态学观测。结果对照组关节软骨基质较均匀、致密,骺板软骨从骨骺到干骺端结构完好,软骨基质呈弱嗜酸性,细胞排列保持良好的层次;幼鼠低剂量组可见软骨细胞变性,坏死,排列紊乱。高剂量组可见软骨细胞溶解、甚至消失。健康细胞与受损细胞交互排列,呈现DLD现象。骨小梁数目减少,且骨小梁变细、连接中断,立体网状结构破坏,且随剂量的增加骨小梁的破坏也愈加严重,甚至骨小梁大片消失;成年鼠可见软骨细胞变性坏死,高剂量组有的细胞核溶解、消失。电镜可见基质内有较小的、大小均匀的、电子密度高的颗粒状物质,线粒体肿胀和固缩兼有,并见大小不等的空泡,脊破坏或消失。高剂量情况下,细胞核形态不规则、固缩,染色质边聚,关节软骨胶原网络结构破坏。结论 DON可导致Wistar大鼠骨和软骨损伤,其损伤结局与骨的发育阶段有关:在干骺闭合前,DON除可致软骨损伤外,还可致骨发育障碍,甚至停滞;在干骺闭合以后其损伤仅表现在软骨上,提示DON可能是大骨节病和骨关节炎等骨关节病的原因。     

Cecal parameters of rats fed diets containing grapefruit polyphenols and inulin as single supplements or in a combination

OBJECTIVE: We compared the effects of grapefruit flavonoids and inulin, as single dietary components or in a combination, on cecal fermentation in rats adapted to a semipurified diet. METHODS: The experimental diets contained 0.3% flavonoid extract and 5% or 10% inulin and a combination of both supplements. The large bowel metabolism assessment was based on cecal parameters: bulk effect, pH, microbial enzymes activity, and short-chain fatty acid production. RESULTS: Both supplements induced significant enlargement of the cecal digesta weight. Acidification of cecal digesta was more pronounced, with a higher inulin addition to the diet. Cecal pH was the highest with the flavonoid-rich diets and lowest in the case of a simultaneous addition of flavonoids and a high content of inulin. The flavonoid extract applied as a single dietary supplement was observed to decrease the activity of bacterial beta-glucosidase and beta- and alpha-galactosidases in the cecal digesta. In contrast, addition of the grapefruit extract to inulin-containing diets increased the activity of alpha-glucosidase, alpha-galactosidase, and beta-galactosidase. Great accumulation of cecal digesta in rats consuming the flavonoid-diet caused a considerable increase in the short-chain fatty acid pool, mainly acetic acid. Inulin added to the diet decreased the excessive enlargement of digesta caused by dietary flavonoids. Dietary addition of inulin to the flavonoid-diet also normalized hydration of cecal digesta and significantly decreased the pH of digesta. The presence of polyphenols in the inulin-containing diets did not change total short-chain fatty acid production in the cecum of rats. CONCLUSION: Our results suggested that simultaneous intake of inulin and polyphenols can decrease the detrimental effects of the latter on cecal fermentation.     

白鲜皮提取物抗湿疹实验研究

目的：通过动物实验模型观察白鲜皮提取物的抗过敏和止痒作用。方法：建立动物瘙痒和Ⅳ型变态反应模型,以致痒组胺总量和小鼠耳廓肿胀度为指标,观察白鲜皮提取物抗过敏疗效。结果：白鲜皮提取物高、中剂量组致痒阈值高于空白对照组,白鲜皮提取物高剂量组较空白对照组显著降低2,4-二硝基氯苯致敏所导致的小鼠耳廓肿胀度。结论：白鲜皮提取物可以对抗Ⅳ型变态反应和提高致痒阈值,对湿疹的治疗有一定意义。