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1.
目的 探讨大肠癌细胞(LoVo株)热休克蛋白70(Heat shock protein70,HSP70)热应激时表达规律,为进一步研究HSP70在TNF-α诱导大肠癌细胞凋亡中的作用提供了实验依据。方法 培养大肠癌细胞(LoVo),热应激(42℃,30min)后分别培养2、5、8、11h,然后运用免疫组化、Westemblot方法分析应激后不同时间细胞内HSP70的表达和分布。结果 热应激2h后H 相似文献
2.
目的建立人大肠癌LoVo细胞多药耐药细胞株LoVo/5-FU,并探讨其生物学特性及耐药机制。方法人大肠癌细 胞系 LoVo在体外经 2.5μg/ml5-氟尿嘧啶(5-FU)作用,成功诱导 LoVo/5-FU耐药细胞株。体外细胞毒性实验观察它 们对5-FU、丝裂酶素(MMC)、阿霉素(ADM)、顺铂(DDP)、氨甲喋呤(MTX)和阿糖胞苷(AraC)等6种药物的敏感性。 用噻唑蓝(MTT)法、光镜及扫描电镜观察两种细胞形态及结构并绘制出细胞体外生长曲线。免疫组化LSAB法检测细 胞中P26-Bcl-2的表达。应用原位DNA末端转移酶标记法检测5-FU在两种细胞中诱导的细胞凋亡。结果LoVo/5-FU 细胞株对5-FU、MMC和ADM均有耐药性,且对5-FU的耐药程度较亲本细胞提高。与亲本细胞相比,耐药细胞株生长 慢,倍增期延长,汇合密度低,异型性明显。免疫组化LSAB法提示,LoVo/5-FU细胞的凋亡与P26-Bcl-2过度表达有 关。LoVo细胞原位DNA末端转移酶标记阳性率高于LoVo/5-FU。结论LoVo/5-FU多药耐药细胞株耐药性稳定,在相 同条件下与敏感细胞株LoVo相比,细胞凋亡受到抑制,提示LoVo/ 相似文献
3.
人胃癌细胞热耐受性与热休克蛋白70的关系 总被引:2,自引:0,他引:2
目的观察41℃60min温热作用后人胃癌细胞BGC-823热耐受性和HSP70表达的变化。方法41℃60min温热作用后不同时间,测定细胞43℃60min温热再作用的克隆形成率,并进行细胞HSP70免疫组化分析。结果①41℃诱导后即时细胞已形成较高的热耐受性,4h有所降低,8h达到高峰(P<0.01),24h恢复近原有水平;②BGC-823细胞常温下表达少量HSP70,并且主要分布于胞浆内;41℃诱导后即时HSP70的表达量并未增加,但核内染色加深(P<0.01);诱导后4h开始增加(P<0.01),8~16h达到高峰,并主要在核内(P<0.01);24h衰减至近原有水平(P>0.05)。结论41℃温热诱导后BGC-823细胞形成的热耐受性包括两部分:一部分为即时、非HSP70依赖的,另一部分为HSP70依赖的热耐受性。 相似文献
4.
用放射自显影和免疫印迹方法探讨了热应激蛋白(HSP)在多种细胞株中的诱导、定位和分布。结果发现,与正常情况相比,各种细胞在热休克后均可诱导合成大量HSP82.3、HSP69.5和HSP26.8,其合成高峰为受热后4~6h。在正常情况下,HSP69.5和HSP26.8主要位于胞浆,在热休克后(43~45℃)迅速向核内迁移,在恢复期,它们又回到胞浆。 相似文献
5.
热应激蛋白的诱导,定位与再分布研究 总被引:4,自引:0,他引:4
用放射自显影和免疫印迹方法探讨了热应激蛋白(HSP)在多种细胞株中的诱导、定位和分布。结果发现,与正常情况相比,各种细胞在热休克后均可诱导合成大量HSP82.3、HSP69.5和HSP26.8,其合成高峰为受热后4-6h。在正常情况下,HSP69.5和HSP26.8主要位于胞浆,在热休克后(43-45℃)迅速向核内迁称,在恢复期,它们又回到胞浆。 相似文献
6.
人大肠癌多药耐药细胞LoVo/5—FU的建立及其生物学特性的初步研究 总被引:2,自引:0,他引:2
目的 建立人大肠癌LoVo细胞多药耐药细胞株LoVo/5-FU,并探讨其生物学特性及耐药机制。方法 人大肠癌细胞系LoVo在体外经2.5ug/ml5-氟尿嘧啶(5-FU)作用,成功诱导LoVo/5-FU耐药细胞株。体外细胞毒性实验观察它们对5-FU、丝裂酶素(MMC)、阿霉素(ADM)、顺铂(DDP)、氨甲喋呤(MTX)和阿糖胞苷(AraC)等6种药物的敏感性。用噻唑蓝(MTT)法、光镜及扫描电镜观察两种细胞形态及结构并绘制出细胞体外生长曲线。免疫组化LSAB法检测细胞中P26-Bcl-2的表达。应用原位DNA末端转移酶标记法检测5-FU在两种细胞中诱导的细胞凋亡。结果 LoVo/5-FU细胞株对5-FU、MMC和ADM均有耐药性,且对5-FU的耐药程度较亲本细胞提高。与亲本细胞相比,耐药细胞株生长慢,倍增期延 相似文献
7.
热休克(42℃,2h)或H2O2预处理,均能在蛋白翻译合成水平使牛肺动脉内皮细胞(BPAECs)中的HSP70增多;在基因围录水平使BPAECs中HSP70mRNA含量增加。蛋白激酶C(PKC)抑制剂--Staurosporine(STP)能显著减少热休克和H2O2诱导的HSP70合成及HSP70mRNA转录的增加。提示PKC在热休克和H2OS诱导的热休克基因表达的信息传递中具有重要作用。 相似文献
8.
目的:研究热化疗诱导大肠癌(LoVo)细胞凋亡及其与bcl-2基因表达的关系,探讨热化疗治疗大肠癌的机制。方法:应用热疗联合顺铂(DDP)在不同药物浓度、时间和温度条件下诱导LoVo细胞凋亡,以流式细胞仪检测其凋亡率及bcl-2基因的表达。结果:随着药物浓度的升高、作用时间的延长,细胞凋亡率增加;热疗联合DDP所致凋亡率大于单纯化疗组和单纯热疗组之和;随着药物浓度的升高,bcl-2的表达下凋;各处 相似文献
9.
热休克(42℃,2h)或H_2O_2预处理,均能在蛋白翻译合成水平使牛肺动脉内皮细胞(BPAECs)中HSP70增多;在基因转录水平使BPAECs中HSP70mRNA含量增加。蛋白激酶C(PKC)抑制剂──Staurosporine(STP)能显著减少热休克和H_2O_2诱导的HSP70合成及HSP70mRNA转录的增加。提示PKC在热休克和H_2O_2诱导的热休克基因表达的信息传递中具有重要作用。 相似文献
10.
bcl—xS基因对化疗引起的大肠癌细胞凋亡的调节作用 总被引:1,自引:0,他引:1
目的 探讨bxl-xS基因对化疗后大肠癌细胞生物学行为的影响。方法 用基因转染方法进行LoVo细胞bcl-xS基因转染,然后用MTT和ELISA法检测bcl-xS转染细胞对化疗药MMC及5-FU敏感性的影响和作用机制,结果 bcl-xS转染可增强LoVO细胞对化疗药MMC及5-FU的敏感性,与经neo转染的细胞相比有显著差异(P〈0.05),ELISA细胞死亡试剂盒研究发现,LoVo细胞对化疗药物 相似文献
11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
12.
Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
13.
Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
15.
Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献
16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption. 相似文献
17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
18.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献
19.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center. 相似文献
20.
Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine
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A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献