7种治疗心血管疾病的中成药抗氧自由基作用的研究

利用黄嘌呤/黄嘌呤氧化酶体系或碱性二甲基亚砜生成的O_2~.与鲁米诺作用的化学发光体系,研究7种中成药水提物抗氧自由基的作用。结果表明:7种中成药水提物对酶体系产生的O_2~.都有抑制作用,其中3种对非酶体系产生的O_2~.有抑制作用,而另4种则表现出负的抑制作用。     

人参皂甙等清除超氧阴离子自由基的研究

筛选自由基清除剂常用黄嘌呤氧化酶氧化黄嘌呤产生O_2~-·,但此体系中黄嘌呤氧化酶易被药物抑制,致使结果分不清是由于药物清除O_2·~-的作用还是药物抑制黄嘌呤氧化酶而     

人参皂甙等清除超氧阴离子自由基的研究

筛选自由基清除剂常用黄嘌呤氧化酶氧化黄嘌呤产生O_2~-·,但此体系中黄嘌呤氧化酶易被药物抑制,致使结果分不清是由于药物清除O_2·~-的作用还是药物抑制黄嘌呤氧化酶而     

化学发光法测定培养细胞中超氧化物歧化酶活力

超氧化物歧化酶(Superoxide Dismuterse,SCD)(EC.1.15.1.1.)存在于需氧细胞中。当细胞中有超氧阴离子(O_2)存在时,该类酶可催化反应产生氧和H_2O_2,从而起到抗氧毒性、抗衰老、抗炎症等作用。随着自由基医学和自由基生物学的发展,SOD的作用越来越受到人们的重视。基于细胞培养技术在生物学研究领域中的广泛开展。有必要建立一种离体培养细胞中SOD活力的测定方法。为此,本文介绍一种体外培养细胞中SOD活力的化学发光测定法。发光体系为黄嘌呤氧酶(Xanthine Oxidase,XO)→次黄嘌呤(Hypoxanthine,HX)→鲁     

田七茎叶提取物对氧自由基的清除作用

用黄嘌呤-黄嘌呤氧化酶体系产生的 O_2~(?)或碱性连苯三酚自氧化产生的 O_2~(?)与鲁米诺作用的化学发光法,测定田七茎叶提取物对氧自由基的清除作用。结果表明:田七茎叶提取物有清除 O_2~(?)作用,且水溶性物清除能力比醇溶性物强。     

甘草类黄酮对脂质过氧化和活性氧自由基的作用

本文用比色法测定过氧化脂质研究甘草类黄酮(FG)的抗脂质过氧化作用,并用化学发光法和自旋捕集技术检测该药对O_2~-和OH的清除作用.体外实验表明:FG2.8～25μg/ml或2.58～258μg/ml分别对碱性二甲基亚砜或黄嘌呤/黄嘌呤氧化酶体系生成的O_2~-有显著清除作用;以上作用呈浓度依赖性变化.     

SOD对鼠冷冻伤脑水肿的保护作用

用电子自旋共振技术(ESR)及自旋捕捉剂(PBN)直接检测冷冻伤及伤后应用SOD、甘露醇治疗的脑组织自由基动态改变;同时检测丙二醛(MDA)、SOD活性及脑组织水含量。结果治疗组和对照组相比,自由基、MDA、水含量明显降低(P<0.01)。SOD含量回升(P<0.01);SOD比甘露醇疗效更显著(P<0.05)。提示SOD、甘露醇都有抗自由基作用;超氧阴离子自由基O_2~(?)在病理性自由基反应中具有重要作用。     

氧自由基在休克发病中的作用

<正> 氧自由基是一类具有未配对价电子数、高度活性的原子、原子团或分子。如超氧阴离子自由基((?))、羟基自由基(OH·)、单线氧(~1O_2)和过氧化氢(H_2O_2)等。它们是生物体内新陈代谢的中间产物,也是生物在进化过程中产生的有益的抗微生物系统。然而病理状态下,过度的自由基反应会对机体产生毒性作用。近年来,自由基理论的研究已渗透到医学领域的各个分枝。本文就氧自由基与休克关系方面的研究概况作一综述。     

槲皮素及异槲皮素、芦丁抗自由基活性的比较研究

目的评价3种黄酮类同系化合物槲皮素及其单糖苷异槲皮素和双糖苷芦丁的体外抗自由基作用,进一步研究其构效关系。方法采用H2O2/Fe2 体系,通过Fenton反应生成羟自由基(·OH);采用邻苯三酚自氧化法产生超氧阴离子(O2-)。利用·OH造成肝细胞脂质过氧化以及红细胞膜破裂,观察受试化合物的保护作用,并计算IC50。结果在以上4种体外模型中,3种药物均表现出极强的清除自由基能力。在·OH以及O2-清除实验中,抗氧化活性顺序为芦丁>异槲皮素>槲皮素;在抑制肝匀浆脂质过氧化及红细胞膜破裂实验中,抗氧化活性顺序为槲皮素>异槲皮素>芦丁。结论3种受试黄酮类化合物均具有十分强大的自由基清除作用,且呈现明显的剂量依赖性和糖基结构依赖性,在不同的实验体系中其抗自由基活性强弱顺序不同。     

槲皮素-钼配合物的抗氧化作用

目的:探讨槲皮素-钼配合物的抗氧化作用.方法:分别用邻苯三酚法和水杨酸法测定槲皮素-钼配合物对超氧阴离子自由基(O-2·)和羟基自由基(·OH)的清除作用,并与槲皮素进行比较.结果:槲皮素-钼配合物对 O-2· 和·OH均有明显的清除作用,其对O-2·的清除作用优于槲皮素.结论:槲皮素-钼配合物具有较为显著的抗氧化作用.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.