耳内窥镜下鼓膜修补术临床治疗分析

目的探讨耳内窥镜下鼓膜修补术的临床治疗效果。方法我院治疗的鼓膜穿孔患者38例,均采用耳内窥镜下鼓膜修补术治疗。观察术后患者的鼓膜穿孔的愈合情况和纯音听阈的恢复情况。结果 38例患者均顺利完成手术,其中37例患者术后鼓膜完全愈合,愈合率达到97.37%。术后1个月时,患者的听阈提高(8.75±2.67)dB;术后3个月时,患者的听阈提高(10.38±2.37)dB,术后6个月时,患者的听阈提高(13.79±3.24)dB;术后12个月时,患者的听阈提高(18.65±4.36)dB。术后患者的纯音听阈逐渐增高(P<0.05)。术后无明显的并发症发生。结论采用耳内窥镜下鼓膜修补术治疗鼓膜穿孔,具有疗效确切,手术时间短,患者创伤小,并发症少的特点,值得临床推广。     

耳内窥镜下鼓膜修补术的临床分析

目的 探讨耳内窥镜下鼓膜修补术的临床疗效.方法 选取本院自2010年1月～2011年1月收治的40例行耳内窥镜下鼓膜修补术治疗的鼓膜穿孔患者,并对其临床资料进行回顾性分析.结果 本组40例患者经耳内窥镜下鼓膜修补术治疗后39例患者鼓膜完全愈合,其愈合率为97.5％,术后患者纯音听阈显著提高（P＜0.05）,且术后无一例患者发生眩晕、耳鸣加重、骨传导听力下降、面瘫等严重并发症.结论 给予鼓膜穿孔患者耳内窥镜下鼓膜修补术治疗,具有良好的效果,有助于提高患者纯音听阈,且并发症少,临床效果显著,值得推广和应用.     

耳内镜下自体脂肪移植鼓膜修补术的临床研究

目的探讨自体脂肪移植在鼓膜修补中的适应证及疗效。方法对43例（43耳）鼓膜穿孔患者，采用耳内镜下自体脂肪移植鼓膜修补术，其中大穿孔10例，中、小穿孔33例，术后对患者的疗效进行观察分析。结果全部患者术后随访6个月-1年，发现中、小穿孔疗效明显。结论自体脂肪移植鼓膜修补术适用于中、小鼓膜穿孔，是一种理想的手术方法。     

耳内窥镜下鼓膜修补术的临床效果观察

目的分析耳内窥镜下鼓膜修补术的临床效果。方法回顾分析57例患者的临床资料。结果其中54例患者在实施1次手术治疗后鼓膜完全愈合；2例患者实施了第二次手术治疗之后鼓膜痊愈；1例患者留有小缝隙，是因为移植物皱缩所导致的，因此拒绝再次进行治疗。所有患者在实施手术0．5～2个月时间后鼓膜愈合，实施1次穿孔手术治愈率为94．74％，总治愈率为98．26％。在患者术后3个月进行纯音听力计检查复查显示，其中，45例患者的气骨导差缩小为5～10dB，12例患者的气骨导差缩小为10-15dB。且所有患者在实施手术治疗后均没有出现耳鸣、耳鸣加重或者是面瘫等并发症状。结论耳内窥镜下实施鼓膜修补术为一种安全可靠、微创小、费用低、操作简单的鼓膜修补方法，这种方法在耳鼻喉的临床治疗上具有良好的治疗效果，值得推广应用。     

耳内窥镜下鼓膜修补术临床治疗观察

目的探讨耳内窥镜下鼓膜修补术的疗效及临床分析。方法 2010年6月至2012年6月期间,我院实施的30例耳内窥镜下鼓膜修补术,对其临床资料进行回顾性分析。结果 30例鼓膜穿孔患者,经过耳内窥镜下鼓膜修补术治疗,手术均顺利完成,其中28例愈合,其治愈率达到93.3%,另外2例,由于术后感冒,引发中耳发炎感染,导致穿孔未愈合,经过相应处理后,症状也出现相应好转。术后2周患者的听力就有一定程度的恢复,术后6个月,其听力得到了显著的提高。结论耳内窥镜下实施鼓膜穿孔修补术,具有术野清晰、创伤较小、术后恢复快、治愈率高等特点,值得临床广泛推广。     

耳内镜下嵌入法脂肪鼓膜修补术的临床分析

目的探讨耳内镜下嵌入法脂肪鼓膜修补术的临床应用价值。方法回顾性分析2003年3月至2007年3月间128例(138耳)行自体脂肪鼓膜修补术患者的临床资料,观察不同大小、不同类型鼓膜穿孔的愈合情况。结果全部患者经6个月以上的随访,总愈合率达86.96%;其中小穿孔愈合率为96%,平均听力提高9.8±4.6dB;穿孔大小不同愈合率经χ2检验,χ2=35.29,P<0.01,差异有统计学意义。结论耳内镜下嵌入法脂肪鼓膜修补术是一种简单、安全、微创、有效的方法;对穿孔大小在鼓膜面积5%～30%的中小穿孔、且中鼓室干燥无炎症的患耳成功率高,疗效确切。     

耳内镜下自体脂肪移植鼓膜修补术33例手术配合

鼓膜穿孔是由外伤或者炎症引起,为了有效地促进鼓膜穿孔愈合,缩短愈合时间,减少并发症发生。我院耳鼻咽喉科从2007年11月至2009年9月,在耳内镜下自体脂肪组织移植修补鼓膜穿孔33例,效果满意,现将手术配合报告如下。1资料与方法1.1临床资料33例（33耳）均为紧张部穿孔,左侧26耳,右侧7耳,鼓膜穿孔面积直径2～4mm,其中男10例,女23例,年龄13～61岁,平均30.3岁;外伤性鼓膜穿孔28例,慢性单纯性中耳炎遗留穿孔5例。1.2手术方法患者取平卧位,术耳朝上,常规消毒铺无菌     

耳内窥镜下鼓膜修补术临床分析

目的 观察在临床治疗鼓膜穿孔患者的过程中使用耳内窥镜下鼓膜修补术的临床治疗效果.方法 整理我院100例鼓膜穿孔患者的临床资料,在治疗过程中患者均使用耳内窥镜下鼓膜修补术进行治疗,对患者在治疗过程中的治疗效果及术中、术后的并发症情况进行统计分析,总结在临床治疗过程中使用这种方法的效果.结果 完成手术治疗之后本组患者的整体治疗效果显著,患者当中的中穿孔、小穿孔情况在治疗后5～7周左右痊愈,通过临床听力检测,患者的听力情况恢复良好,与治疗前相比差异明显.结论 在鼓膜穿孔患者的治疗过程中使用耳内窥镜下鼓膜修补术的手术治疗效果显著,在临床治疗过程中可以进行推广使用.     

耳内窥镜下鼓膜修补术临床分析

目的 研究耳内窥镜下鼓膜修补术的临床效果.方法 收集我院治疗的鼓膜穿孔患者120例,对患者进行耳内镜下鼓膜修补术进行治疗,观察治疗效果及不良反应发生情况.结果 本组120例患者当中,鼓膜愈合114例,患者的治疗有效率为96.7%,其手术时间为45 min～1 h左右,治疗过程中患者大穿孔的愈合时间在5～7周左右,中穿孔、小穿孔平均愈合时间2～4周.治疗完成后患者的听力与治疗前相比明显提高.结论 在治疗鼓膜穿孔患者时,可以通过耳内窥镜下鼓膜修补术进行治疗.     

耳内镜下自体脂肪配合透明质酸钠鼓膜修补的疗效观察

目的探讨耳内镜下自体脂肪配合透明质酸钠鼓膜修补的临床应用价值。方法对75例(75耳)临床确诊单纯性鼓膜穿孔患者分为研究组39例(39耳),采用自体脂肪配合透明质酸钠修补鼓膜,对照组36例(36耳)采用补贴法修补鼓膜;观察不同大小的鼓膜穿孔的愈合情况。结果患者随访时间均>6个月,研究组的大穿孔、中穿孔、小穿孔愈合率和总愈合率分别是85%、92%、100%和94%;对照组的大穿孔、中穿孔、小穿孔愈合率和总愈合率分别是16%、21%、50%和33%;平均听力提高:研究组(13±3.6d)B,对照组(5±2.2)dB;研究组治疗效果明显优于对照组,差异有非常显著性有统计学意义(P<0.05)。结论与传统的补贴法修补鼓膜比较,耳内镜下自体脂肪鼓膜配合透明质酸钠鼓膜修补术是一种简便、安全、有效的方法。     

微RNA-802在肥胖人群脂肪组织中的表达及临床意义

目的 探讨微RNA-802(miR-802)在肥胖人群脂肪组织中的表达情况及临床意义。方法 选择2019年3月至2021年7月在枣庄市三家医院因慢性胆囊结石而需手术治疗的病人102例,其中体质量指数(BMI)≥28 kg/m²有59例作为肥胖组,BMI<24 kg/m²有43例作为非肥胖组。手术过程中取皮下脂肪组织,采用实时荧光定量逆转录PCR(RT-qPCR)检测脂肪组织miR-802相对表达量;比较两组人体学指标、血脂、血糖等临床资料;采用Pearson分析肥胖病人脂肪组织miR-802水平与各指标的相关性。结果 肥胖组、非肥胖组病人性别、年龄及血清高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平之间比较,差异无统计学意义(P>0.05),肥胖组病人BMI[(31.81±2.43)kg/m²]、腰臀比(WHR)(1.03±0.07)、空腹血糖(FBG)[(10.51±2.71)mmol/L]、空腹胰岛素(FINS)[(16.54±3.26)mU/L]、胰岛素抵抗指数(HOMA-IR)...     

耳内镜下自体腹壁脂肪修复中央性鼓膜穿孔的临床探讨

目的探讨耳内镜下腹壁脂肪修补中央性鼓膜穿孑L的临床效果。方法回顾性分析64例（68耳）中央性鼓膜穿孔的病例资料，均于耳内镜下行腹壁脂肪修补术，术后随访3—6个月，术后6个月复查纯音测听，观察术后疗效。结果鼓膜一次性愈合63耳；鼓膜部分愈合4耳，予新鲜鸡蛋膜外贴修补后愈合；1耳未愈；一次性治愈率为92．6％（63／68）。结论耳内镜视野清晰，使用简单方便；耳内镜下腹壁脂肪修补中央性鼓膜穿孔临床效果显著，值得在基层医院推广应用。     

Allergen exposure induces inflammation and affects adiponectin levels in adipose tissue

This study investigates whether allergen exposure elevates the risk of diabetes or cardiovascular diseases using acute OVA (Ovalbumin) allergen exposure model. We hypothesize that exposure to allergen can induce adipose tissue inflammation and affect adiponectin levels. An intranasal challenge with OVA male C57BL/6 mice was performed at dose of 6.25, 12.5, 25, 50 and 100 μg, and compared to which challenge with PBS (phosphate buffered saline). Results showed that acute OVA exposure did not only cause airway inflammation in study mice, but also decreased serum adiponectin levels with a dose-response effect. When examining the gonadal adipose tissues, there was no significantly difference of adiponectin mRNA in OVA challenged mice compared to those PBS challenged, but lower inguinal adiponectin mRNA expression was found compared to those PBS-challenged, and had a good relationship with the serum adiponectin. Inguinal adipose tissues of OVA challenged mice, had significantly lower adipose tissue weight, and higher TNF-α expression without statistical significance. Our data indicate that acute OVA exposure appears to affect the characteristics of adipose tissues, and change the adiponectin levels in serum and adipose tissues. Allergen exposure may be considered a potential risk factor for presenting diabetes or cardiovascular diseases.     

大网膜脂肪组织提取液中辅助性T淋巴细胞分泌细胞因子的检测

目的分析测定大网膜脂肪组织提取液中辅助性T淋巴细胞分泌的细胞因子。方法应用流式细胞仪对大网膜脂肪组织提取液及血浆中IFN-γ,TNF-α,IL-2,IL-4和IL-10进行测定分析。结果大网膜脂肪组织提取液内可分泌上述细胞因子,其中TNF-α,IL-2,IL-4和IL-10较血液中各细胞因子含量明显增高（P〈0．05）。结论大网膜脂肪组织可分泌多种细胞因子,可能对腹腔内组织器官瘢痕的形成和发展起重要影响作用。     

重组表皮生长因子联合莫匹罗星在鼓膜修补术中的临床应用效果

目的：探讨重组表皮生长因子联合莫匹罗星在鼓膜修补术中的临床应用效果。方法选择2013年1~12月在本院接受鼓膜修补术治疗的60例患者为研究对象,随机分为2组,各30例,对照组患者行明胶海绵单纯外贴手术,试验组患者行涂有重组表皮生长因子+莫匹罗星混合物的明胶海绵外贴手术,比较两组治疗效果及并发症情况。结果试验组治愈率为93.33%,对照组治愈率为86.67%,两组比较,差异无统计学意义(P跃0.05);试验组并发症发生率为3.3%,低于对照组的20%,差异有统计学意义(P<0.05);两组术后听力改善及鼓膜愈合时间比较,差异有统计学意义(P<0.05)。结论鼓膜修补术中应用重组表皮生长因子联合莫匹罗星,能有效促进创面愈合,减少并发症,提高手术成功率。     

Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice

Dynamic changes in adipose tissue blood flow (ATBF) with nutritional status play a role in the regulation of metabolic and endocrine functions. Activation of the sympathetic nervous system via β-adrenergic receptors (β-AR) contributes to the control of postprandial enhancement of ATBF. Herein, we sought to identify the role of each β-AR subtype in the regulation of ATBF in mice. We monitored the changes in visceral epididymal ATBF (VAT BF), induced by local infusion of dobutamine, salbutamol, and CL316,243 (a selective β1-, β2-, and β3-AR agonist, respectively) into VAT of lean CD-1 mice and global adipose triglyceride lipase (ATGL) knockout (KO) mice, using laser Doppler flowmetry. Administration of CL316,243, known to promote lipolysis in adipocytes, significantly increased VAT BF of CD-1 mice to a greater extent compared to that of the vehicle, whereas administration of dobutamine or salbutamol did not produce significant differences in VAT BF. The increase in VAT BF induced by β3-AR stimulation disappeared in ATGL KO mice as opposed to their wild-type (WT) littermates, implying a role of ATGL-mediated lipolysis in the regulation of VAT BF. Different vascular reactivities occurred despite no significant differences in vessel density and adiposity between the groups. Additionally, the expression levels of the angiogenesis-related genes were significantly higher in VAT of ATGL KO mice than in that of WT, implicating an association of ATBF responsiveness with angiogenic activity in VAT. Our findings suggest a potential role of β3-AR signaling in the regulation of VAT BF via ATGL-mediated lipolysis in mice.     

耳窥镜辅助下应用耳屏软骨-软骨膜进行鼓膜修补术的临床分析

目的讨论耳窥镜辅助下应用耳屏软骨一软骨膜进行鼓膜修补术的临床治疗效果。方法选取本院58例鼓膜穿孔患者采用在耳窥镜辅助下应用耳屏软骨一软骨膜修补鼓膜穿孔，分别在手术后1个月、3个月、6个月后进行复查，对患者鼓膜穿孔的愈合情况、听阈情况进行观察分析。结果58例患者在进行耳屏软骨一软骨膜手术在半年后总的治愈率为98．28％；手术后1个月与手术前听力比较、手术后3个月与手术后1个月听力比较差异有统计学意义（P〈0．05）；比较手术后6个月和手术后3个月的听力情况差异无统计学意义（P〉0．05）。结论耳窥镜辅助下应用耳屏软骨一软骨膜进行鼓膜修补术治疗因各种原因导致的鼓膜穿孔具有良好的临床疗效，患者治愈率高。     

Effect of DHEA on endocrine functions of adipose tissue, the involvement of PPAR gamma

Dehydroepiandrosterone (DHEA), an adrenal steroid, is known to decrease body fat. Thus, it may also alter the endocrine functions of adipose tissue. The aim of this study was to determine if administration of DHEA might influence adiponectin gene expression and secretion from adipose tissue. We demonstrate here the inducing effect of exogenously administered DHEA on adiponectin gene expression in epididymal WAT and adiponectin levels in serum of rats fed a DHEA-containing diet (0.6%, w/w) for 2 weeks, accompanied by a reduction in epididymal adipose tissue mass. A corresponding increase in peroxisome proliferator-activated receptor gamma (PPAR(gamma)) mRNA expression suggests that PPAR(gamma) may be involved in the up-regulation of adiponectin gene expression after DHEA treatment. The presented observations indicate that the positive effects of DHEA, which seems to play a protective role against insulin resistance and atherosclerosis, may be in fact indirect and due to up-regulation of adiponectin gene expression and stimulation of adiponectin secretion from adipose tissue.     

Limitation of adipose tissue enlargement in rats chronically treated with semicarbazide-sensitive amine oxidase and monoamine oxidase inhibitors

Inhibition of semicarbazide-sensitive amine oxidases (SSAO) and monoamine oxidases (MAO) reduces fat deposition in obese rodents: chronic administration of the SSAO-inhibitor semicarbazide (S) in combination with pargyline (MAO-inhibitor) has been shown to reduce body weight gain in obese Zucker rats, while (E)-2-(4-fluorophenethyl)-3-fluoroallylamine, an SSAO- and MAO-B inhibitor, has been reported to limit weight gain in obese and diabetic mice. Our aim was to state whether such weight gain limitation could occur in non-obese, non-diabetic rats and to extend these observations to other amine oxidase inhibitors. Prolonged treatment of non-obese rats with a high dose of S (900 micromol kg(-1) day(-1)) reduced body weight gain and limited white adipose tissue enlargement. When chronically administered at a threefold lower dose, S also inhibited SSAO activity but not fat depot enlargement, suggesting that effects other than SSAO inhibition were involved in adipose tissue growth retardation. However, combined treatment of this lower dose of S with pargyline inhibited SSAO, MAO, energy intake, weight gain and fat deposition. Adipocytes from treated rats exhibited unchanged insulin responsiveness but impaired antilipolytic responses to amine oxidase substrates. Phenelzine clearly inhibited both MAO and SSAO when tested on adipocytes. Obese rats receiving phenelzine i.p. at 17 micromol kg(-1) day(-1) for 3 weeks, exhibited blunted MAO and SSAO activities in any tested tissue, diminished body weight gain and reduced intra-abdominal adipose tissue. Their adipocytes were less responsive to lipogenesis activation by tyramine or benzylamine. These observations suggest that SSAO inhibition is not sufficient to impair fat deposition. However, combined MAO and SSAO inhibition limits adiposity in non-obese as well as in obese rats.