CNTN-1蛋白在早期子宫颈鳞癌中的表达及其与预后的关系

目的探讨神经黏附分子接触蛋白-1(CNTN-1)在子宫颈鳞癌的表达水平与临床病理因素的关系及其预后价值。方法采用免疫组化法PV法检测CNTN-1蛋白在48例子宫颈鳞癌组织、40例子宫颈鳞状上皮内病变(SIL)及20例正常子宫颈组织中的表达情况及定位分布。结合临床病理资料、随访数据和CNTN-1蛋白表达情况对子宫颈鳞癌患者进行预后分析。结果 CNTN-1蛋白在正常子宫颈组织、SIL、子宫颈鳞癌组织中呈递增表达,差异有统计学意义(P 0.05)。在子宫颈鳞癌组,CNTN-1蛋白高表达率与FIGO分期及淋巴结转移有关(P 0.05),与患者年龄及肿瘤分化程度无关(P0.05)。Kaplan-Meier法生存分析和Log-rank检验表明:子宫颈鳞癌组织中CNTN-1蛋白高表达组的患者无瘤生存期较低表达组短,差异有统计学意义(P 0.05)。COX比例风险回归模型预后分析示CNTN-1蛋白的表达、肿瘤分化程度和淋巴结转移是子宫颈鳞癌患者的独立预后因素。结论 CNTN-1蛋白在子宫颈鳞癌的发生发展及转移浸润中起促进作用。     

宫颈鳞癌组织中结缔组织生长因子及转化生长因子-β1的表达及临床意义

目的:探讨结缔组织生长因子(CTGF)蛋白、转化生长因子-β1(TGF-β1)蛋白在宫颈鳞癌组织中的表达及其与临床病理特征的关系,特别是与盆腔淋巴结转移的关系。方法:应用Power Vision免疫组织化学染色法检测正常宫颈组织(10例)、CINⅡ~Ⅲ组织(20例)和宫颈鳞癌组织(98例)中CTGF蛋白及TGF-β1蛋白的表达情况,并比较两种蛋白的阳性表达率与宫颈鳞癌的不同临床病理因素间的关系及其与盆腔淋巴结转移的关系。结果:1CTGF蛋白、TGF-β1蛋白在正常宫颈、CINⅡ~Ⅲ、宫颈鳞癌中的阳性表达率呈现递增的趋势,三者比较差异有统计学意义(P0.05)。2宫颈鳞癌中CTGF蛋白表达与TGF-β1蛋白表达之间呈正相关(r=0.643,P0.05)。3宫颈鳞癌组织中CTGF、TGF-β1蛋白的阳性表达率在不同的组织学分化程度、盆腔淋巴结有无转移和不同临床分期间比较,差异有统计学意义(P0.05)。4多因素非条件Logistic回归分析示:CTGF蛋白、年龄、组织分化程度及肿瘤直径是影响宫颈鳞癌盆腔淋巴结转移的独立危险因素(P0.05)。结论:CTGF蛋白、TGF-β1蛋白参与宫颈鳞癌的恶性程度进展,且CTGF可作为宫颈鳞癌盆腔淋巴结转移的独立危险因素之一。     

S100A14与RAGE在子宫颈鳞癌组织中的表达及临床意义

目的:探讨钙结合蛋白S100A14与晚期糖基化终产物受体(RAGE)在宫颈鳞癌组织中的表达及临床意义。方法:免疫组化法检测S100A14与RAGE在23例正常宫颈、76例宫颈上皮内瘤变(CIN)及102例宫颈鳞癌组织中的表达,探讨S100A14与RAGE表达与宫颈癌临床病理指标的关系。对102例宫颈鳞癌患者进行术后随访,分析S100A14与RAGE表达与预后的关系。结果:S100A14和RAGE在正常宫颈到宫颈鳞癌组织中的表达均呈递增趋势(P<0.05)。宫颈鳞癌中S100A14和RAGE表达与FIGO分期、淋巴结转移和深层间质浸润有关(P<0.05);RAGE表达与肿瘤大小有关,与分化无关;但S100A14与分化有关,与肿瘤大小无关。S100A14与RAGE在宫颈鳞癌中表达呈正相关(P<0.05)。S100A14和RAGE高表达组分别与低表达组相比较,生存率均明显降低(P<0.05)。结论:S100A14与RAGE在宫颈鳞癌中均表达上调,其表达升高与宫颈鳞癌的发生、浸润及转移相关,可能成为治疗宫颈鳞癌的新靶点和评估预后的有力指标。     

S100A4蛋白和上皮钙粘素在宫颈鳞癌中的表达及意义

目的:探讨S100A4蛋白和上皮钙粘素(E-cad)在宫颈鳞癌组织中的表达及其临床意义。方法:采用免疫组织化学SP法检测65例宫颈鳞癌组织和10例正常宫颈组织中S100A4蛋白和E-cad的表达水平。结果:(1)宫颈鳞癌组织中S100A4蛋白和E-cad的表达率分别为35.4%(23/65)和33.8%(22/65);与正常宫颈组织比较,差异具有显著性(P<0.01);(2)S100A4蛋白在宫颈鳞癌中的表达与临床分期及淋巴结转移有关(P<0.05);与组织学分级无关(P>0.05);E-cad表达与淋巴结转移和组织学分级有关(P<0.05),与临床分期无关(P>0.05);(3)S100A4蛋白和E-cad在宫颈鳞癌组织的表达呈负相关(r=-0.257,P<0.05)。结论:S100A4蛋白和E-cad与宫颈鳞癌浸润和转移相关;S100A4蛋白可作为判定宫颈鳞癌临床病理特征的重要指标之一。     

宫颈鳞状上皮细胞癌组织中钙激活性中电导钾离子通道蛋白产物表达分析

目的探讨正常宫颈和宫颈癌组织中钙激活性中电导钾离子通道(IKCa1)与人类宫颈鳞状上皮细胞癌的相关性。方法取2007年10月至2008年4月泸州医学院附属医院手术治疗的30例不同分化程度的宫颈鳞状上皮细胞癌患者病变部位组织作为实验组,同时期18例正常宫颈上皮组织作为对照组,分别采用免疫组织化学方法检测上述组织中IKCa1蛋白产物表达部位及表达水平。结果 IKCa1在30例宫颈鳞状上皮细胞癌组织细胞核及细胞膜上均呈阳性表达,阳性表达率为100.0%;18例正常宫颈上皮组织中仅1例在鳞状上皮部位细胞膜部位有较弱表达,阳性表达率为5.6%,IKCa1蛋白产物在宫颈鳞状上皮细胞癌组织中的表达程度及表达部位均与正常宫颈组织中存在明显差异(Z=-6.109,P=0.00,P<0.05)。在不同分化程度的宫颈鳞状上皮细胞癌组织中,高分化鳞癌组阳性表达率为40.0%,中分化鳞癌组阳性率为90.0%,低分化鳞癌组阳性率为100.0%;IK-Ca1蛋白产物表达程度与癌细胞分化程度呈负相关(χ2=8.4,P<0.05)。结论宫颈鳞状上皮细胞癌组织中IK-Ca1蛋白产物的表达明显高于正常宫颈组织;且IKCa1蛋白产物表达水平与癌细胞的分化程度呈负相关;过高表达的IKCa1与宫颈鳞状上皮细胞癌的发生和发展可能存在重要联系。     

宫颈癌患者肿瘤组织中ANGPTL4和NRIP1蛋白表达水平及与预后的关系

目的 探究宫颈癌患者肿瘤组织中血管生成素样蛋白4(ANGPTL4)和核受体相互作用蛋白1(NRIP1)蛋白表达水平及与预后的关系。方法 选择2017年1月至2019年1月南通大学附属肿瘤医院收治的98例宫颈癌患者,检测并比较患者癌组织及癌旁组织ANGPTL4和NRIP1蛋白表达,分析二者表达与临床病理学特征及预后的关系。结果 与癌旁组织相比,宫颈癌组织中ANGPTL4和NRIP1的蛋白表达阳性率均更高（P <0.05）;宫颈癌组织中ANGPTL4和NRIP1蛋白表达与肿瘤最大径、FIGO分期、肿瘤浸润深度、淋巴结转移情况有关（P <0.05）,NRIP1蛋白表达还与分化程度有关（P <0.05）。截止2022年1月随访结束,98例患者均获得随访,生存64例（65.31%）、死亡34例（34.69%）,ANGPTL4、NRIP1蛋白阳性表达者与二者对应阴性表达者存活率曲线比较差异有统计意义（P <0.05）。结论 宫颈癌患者肿瘤组织中ANGPTL4和NRIP1蛋白表达阳性率均高于癌旁组织,二者表达情况与肿瘤最大径、FIGO分期、肿瘤浸润深度、淋巴结转移情况有关,且...     

nm23-H1在子宫颈原位癌及浸润癌中的表达

目的探讨nm23-H1表达与宫颈鳞癌发展转移的关系.方法采用免疫组织化学方法检测23例宫颈原位癌及55例浸润鳞癌中nm23-H1的表达情况.结果原位癌nm23-H1阳性表达率高于浸润癌(P＜0.05).浸润癌中,nm23-H1阳性表达率随FIGO临床分期升高而有下降趋势,Ⅰ、Ⅱ期与Ⅲ期比较,差异显著(P＜0.05);有淋巴结转移者其原发灶阳性表达率明显低于无淋巴结转移者(P＜0.01);组织学分化程度高者阳性率高于分化程度低者(P＜0.05).结论nm23-H1基因可抑制宫颈癌发展及淋巴结转移.     

细胞质胸苷激酶在维吾尔族妇女宫颈癌的表达及临床意义

目的:探讨细胞质胸苷激酶(TK1)在宫颈鳞癌的表达及其临床意义。方法:制备40例维吾尔族妇女子宫颈癌及正常宫颈组织中总RNA,用RT-PCR方法分析TK1基因mRNA的表达水平,用免疫组化(SP)法检测其相应蛋白表达。结果:(1)维吾尔族妇女宫颈癌组织中TK1 mRNA及蛋白阳性检出率分别为67.5%及60.5%,对照组宫颈组织中检出率为26.7%及8.3%。宫颈癌组织中TK1 mRNA及蛋白阳性表达明显高于对照组的宫颈组织(P0.05);(2)在宫颈癌组织中TK1 mRNA及蛋白表达:不同临床分期及分化程度之间无显著差异(P0.05),但是,有淋巴结转移患者组织中阳性表达明显高于无淋巴结转移者,肿瘤直径≥4cm患者阳性表达明显高于肿瘤直径4cm的患者(P0.05)。结论:TK1在维吾尔族妇女宫颈鳞癌中高表达,对宫颈癌的诊断有重要的参考价值,TK1高表达可能与宫颈癌的进展及预后有关。     

ALDH1和ABCG2在宫颈鳞癌中的表达及其临床意义

目的:检测肿瘤干细胞标志物乙醛脱氢酶1(ALDH1)和三磷酸腺苷结合盒转运蛋白G2(ABCG2)在不同程度宫颈病变组织中的表达情况。方法:应用免疫组化SP法检测76例宫颈鳞癌、52例宫颈高级别鳞状上皮内病变(HSIL)、37例慢性宫颈炎患者的石蜡组织中ALDH1和ABCG2的表达情况,并进行比较分析。结果:1ALDH1和ABCG2在慢性宫颈炎、HSIL、宫颈鳞癌组织中的阳性表达率分别为5.41%(2/37)和8.11%(3/37)、23.08%(12/52)和28.85%(15/52)、53.95%(41/76)和61.84%(47/76),两者在宫颈鳞癌组织中的阳性表达率明显高于HSIL组织、慢性宫颈炎组织,差异均有统计学意义(P0.05)。2ALDH1在宫颈鳞癌中表达水平与肿瘤分化程度、是否存在淋巴结转移有关(P0.05),ABCG2在宫颈鳞癌中表达水平与宫颈癌的临床分期、肿瘤细胞的分化程度及是否有淋巴结转移有关(P0.05)。3宫颈鳞癌组织中ALDH1和ABCG2表达水平水平呈正相关(r=0.535,P0.05)。结论:ALDH1和ABCG2在宫颈鳞癌的表达升高,可能在宫颈鳞癌的发生、发展、转移中起作用。ALDH1和ABCG2在宫颈鳞癌的发展过程中可能起协同作用。     

子宫颈鳞癌中RON表达及其与预后的关系

目的:探讨子宫颈鳞癌中RON表达与临床病理指标的联系及其预后价值。方法:采用免疫组化法检测RON在子宫颈鳞癌、子宫颈上皮内瘤变及慢性宫颈炎中的表达情况及定位分布,分析其与临床病理因素的关系,同时综合临床病理资料及随访数据对子宫颈鳞癌患者进行预后分析。结果:RON蛋白在正常宫颈组织、CIN、宫颈鳞癌组织中的高表达率分别为10%、37.5%、69.6%。宫颈鳞癌组织中RON蛋白高表达与FIGO分期、淋巴结转移有关(P0.05),而与患者年龄及肿瘤的分化程度无关(P0.05)。Kaplan-Meier法进行单因素生存分析,RON蛋白表达阳性患者及阴性患者的无瘤生存期分别为(23.27±2.10)月、(37.33±2.30)月;Log-rank检验结果显示,两组患者的无瘤生存期比较,差异有统计学意义(χ2=13.510,P0.001)。结论:RON蛋白在宫颈癌的发生、发展、临床分期与淋巴结转移中发挥了重要作用,有望成为预测宫颈癌病情进展的一个潜在的分子标记物。     

Blood lead and cadmium and birth weight among sub-arctic and arctic populations of Norway and Russia

BACKGROUND: Delivering women and their newborns in the Kola Peninsula of Russia and the neighboring arctic area of Norway were studied to explore relationships between maternal cadmium and lead status and birth weight as a pregnancy outcome. METHODS: Life-style information, maternal blood and cord blood specimens were collected from 50 consecutive mother-infant pairs from hospital delivery departments in three Russian and three Norwegian communities. Pregnancy outcomes were verified by consulting medical records. Lead and cadmium were determined in the blood samples by electrothermal atomic absorption spectrometry. RESULTS: The median blood-cadmium concentration for the Russian mothers was 2.2 nmol/L (n = 148) versus 1.8 nmol/L in the Norwegian group (n = 114, p = 0.55). A weak association was observed between maternal cadmium and amount smoked (r = 0.30, p<0.001); no correlation was found between maternal blood cadmium and birth weight. The corresponding maternal lead values were 0.14 (Russia) and 0.06 micromol/L (Norway), p<0.001. The latter lead concentration constitutes one of the lowest adult population values reported to date. Maternal and cord blood lead levels were strongly correlated (r = 0.88, p<0.001). In a multivariate linear regression model, maternal blood lead was recognized as a negative explanatory variable (p<0.05) for birth weight and child's body mass index (BMIC), with or without adjustment for gestational age. A similar association was suggested by ANOVA-analysis of maternal blood lead by quartiles. CONCLUSION: Maternal blood-lead level as an environmental factor is an apparent predictor of low birth weight and BMIC. It reduced substantially the contribution of a country factor in explaining the observed differences in birth weight.     

Pharmacokinetics and endometrial and tubal tissue penetration of cefalotin and cefazolin

The pharmacokinetics and concentrations of the two antibiotics cefazolin and cefalotin were studied during gynecologic operations in endometrial and tubal tissue. The patients received 0.05 g/kg of the antibiotics by intravenous injection. Under the given conditions, pharmacokinetic calculation of the plasma elimination gave half-lives of 24.8 min for cefalotin and of 63 min for cefazolin. Fitting of the tissue levels to the Bateman function showed that the two antibiotics diffuse rapidly into both tubal and endometrial tissue and attain peak concentration levels between 10 and 25 min. In both tissues the concentrations of cefazolin were higher than those of cefalotin. Higher tissue concentrations of cefazolin could also be demonstrated in experiments of longer duration.     

Toll样受体与子宫内膜及子宫内膜异位症

子宫内膜异位症(EMs)发病机制尚未完全阐明.大量研究表明,免疫因素在EMs的发病机制中起重要作用.EMs免疫应答异常主要是巨噬细胞数量和活性增加及其分泌产物,如生长因子、细胞因子和血管生成因子的改变.Toll样受体(TLRs)识别特异性的病原体相关分子模式,启动和介导免疫应答,在固有免疫中发挥重要作用,并诱导产生适应性免疫反应.TLRs在正常子宫内膜中的生理作用以及在EMs中的相关研究已逐步开展,对其深人认识和研究将为EMs诊断、治疗和预后判断提供新思路和手段.     

Reproductive and menstrual factors and risk of peritoneal and ovarian endometriosis

OBJECTIVE: Between 1987 and 1989 data were collected to evaluate risk factors for pelvic endometriosis. DESIGN: A case-control study was conducted on 241 cases with laparoscopically or laparotomically confirmed peritoneal or ovarian endometriosis consecutively admitted to three teaching hospitals in Northern Italy. The control group consisted of 437 women admitted to hospitals for acute conditions covering similar catchment areas. RESULTS: Compared with nulliparous women, the risk of endometriosis decreased with increasing number of births: the point estimates were 0.4 and 0.3, respectively, for those with one and two or more births (X2(1) trend = 50.3, P less than 0.001). No relation emerged with age at first birth and spontaneous miscarriages. Relative to women whose menarche occurred at age 11 or younger, the risk of endometriosis was slightly lower in those who experienced later menarche, but the trend in risk was not significant. Women with irregular menstrual cycles showed a lower frequency of the disease (relative risk, 0.3; 95% confidence interval, 0.2 to 0.5). The role of various factors was largely similar for different disease locations (ovary, peritoneum, and both) and indication for diagnostic surgery (sterility, pelvic pain, and other reasons). CONCLUSIONS: This study found that parity and irregular/long menses lower the risk of endometriosis. These findings were similar in different subgroups of disease location and indication for surgery, giving strong evidence of the consistency of the general results.