乳腺癌组织中第Ⅷ因子相关抗原和组织蛋白酶D与癌转移及？…

目的 探讨乳腺癌组织中第Ⅷ因子相关抗原和组织蛋白酶Ｄ在其转移、预后中的关系。方法 采用免疫组化ＳＰ法对３３例乳腺癌进行第Ⅷ因子相关抗原（Ｆ８）和组织蛋白酶Ｄ（Ｃａｔｈ－Ｄ）染色。结果 ３３例乳腺癌微血管Ｆ８染色均阳性（１００％）。癌组织内染色阳性在７０％以上者７例（２１．２１％）,癌周组织阳性在７０％以上者２５例（７１．６７％）,两者有明显差异（Ｐ〈０．０１）。Ｆ８表达与腋窝淋巴结转移有相关性（Ｐ     

C—erbB—2和组织蛋白酶D在食管癌中的表达及相关性研究

目的：研究Ｃ－ｅｒｂＢ－２和组织蛋白酶Ｄ（Ｃａｔｈ－Ｄ）表达与食管癌分级、浸润、转移和预后的关系,以及ＣｅｒｂｂＢ－２表达与Ｃａｔｈ－Ｄ表达的相互关系。方法：应用微波－ＳＰ免疫组化法：检测６５例食管鳞状细胞癌组织中Ｃ－ｅｒｂＢ－２和ＰＣＡＮ表达水平。结果：在食管癌中Ｃ－ｅｒｂＢ－２和Ｃａｔｈ－Ｄ表达阳性率分别为５０．８％和６４．６％。ＣｅｒｂＢ－２和Ｃａｔｈ－Ｄ表达与肿瘤分级、浸润、淋巴结转移和预     

P53,C—erbB—2,组织蛋白酶D和C—myc蛋白表达及与乳腺癌生？…

目的：检测乳腺癌Ｐ５３、Ｃ－ｅｒｂＢ－２、组织蛋白酶Ｄ和Ｃ－ｍｙｃ癌基因蛋白在乳腺癌的表达并了解它们与乳腺癌生物学行为的关系。方法：对乳腺癌７６例和乳腺良性病变２１例进行Ｐ５３、Ｃ－ｅｒｂＢ－２、组织蛋白酶Ｄ和Ｃ－ｍｙｃ蛋白免疫组化检测。结果：ａ．Ｐ５３和Ｃ－ｅｒｂＢ－２仅在乳腺癌有表达。组织蛋白酶Ｄ在乳腺良恶性病变表达率存在显著差异（Ｐ〈０．０１）。Ｐ５３和组织蛋酶Ｄ表达率随乳腺癌组织学分级升高     

乳腺癌细胞rasP21基因表达水平检测的临床意义

应用免疫组化方法检测了３７例乳腺癌、２５例良性乳腺疾病和１０例正常乳腺组织ｒａｓ癌基因产物Ｐ２１的表达水平。结果显示：乳腺癌Ｐ２１的阳性反应率（７０．３％）远高于良性乳腺疾病（８％）及正常乳腺（０％），且Ｐ２１表达水平随肿瘤体积、局部浸润和淋巴结转移的扩展而升高（Ｐ＜０．０１）。癌组织分化低者Ｐ２１表达水平亦较高（Ｐ＜０．０５）。提示Ｐ２１过度表达是乳腺癌预后不良的征象。     

组织蛋白酶D、P糖蛋白及Her-2与贲门癌预后关系的研究

探讨组织蛋白酶Ｄ（Ｃａｔｈ－Ｄ）、Ｐ糖蛋白（Ｐ－ｇｐ）及Ｈｅｒ－２与贲门癌治疗预后的关系。方法应用免疫组织化学的方法，检测了４３例贲门癌经手术切除的新鲜癌组织标本中的Ｐ糖蛋白、组织蛋白酶Ｄ及Ｈｅｒ－２的表达水平，并结合其病理类型及淋巴结转移进行了综合判定。结果Ｐ糖蛋白及Ｈｅｒ－２的表达与贲门癌的淋巴结转移无统计学意义，（Ｐ＞０．０５），而组织蛋白酶Ｄ的表达与贲门癌的淋巴结转移有显著相关（Ｐ＜０．０１），同时组织蛋白酶Ｄ阳性者其Ｐ糖蛋白及Ｈｅｒ－２的表达也明显高于组织蛋白酶Ｄ阴性者。贲门癌的分化程度与Ｐ糖蛋白及组织蛋白酶Ｄ的表达无显著差异，而与Ｈｅｒ－２的表达却有显著相关（Ｐ＜０．０５）。结论组织蛋白酶Ｄ阳性的贲门癌易发生淋巴结转移，Ｈｅｒ－２阳性者恶性度高且预后差，临床上对上述病人应给予特殊针对性的治疗方案。     

CD44v6、nm23-H1在乳腺癌中的表达及与淋巴结转移的关系

目的探讨ＣＤ４４ｖ６、ｎｍ２３－Ｈ１基因蛋白与乳腺癌淋巴结转移的关系。方法应用免疫组织化学ＳＰ法检测４６例乳腺癌组织中ＣＤ４４ｖ６,ｎｍ２３－Ｈ１基因蛋白的表达。结果ＣＤ４４ｖ６阳性检出率为６９．６％（３２／４６）,其中淋巴结受累乳腺癌阳性率为７２％（１８／２５）,无淋巴结转移组检出率为６６．７％（１４／２１）,两者比较差异无显著性（Ｐ＞０．０５）,但淋巴结受累乳腺癌的ＣＤ４４ｖ６强阳性表达检出率为６４％（１６／２５）,明显高于淋巴结未受累乳腺癌的强阳性表达检出率３３．３％（７／２１）（Ｐ＜０．０５）;ｎｍ２１－Ｈ１阳性表达率为７１．７％（３３／４６）,其中淋巴结受累乳腺癌和未受累乳腺癌中的阳性检出率分别为８０％（２０／２５）、６１．９％（１３／２１）,前者略高于后者,但差异无显著性（Ｐ＞０．０５）。结论ＣＤ４４ｖ６、ｎｍ２３－Ｈ１异常表达可能是乳腺癌发生、发展的重要分子学改变,ＣＤ４４ｖ６强表达可能对判断乳腺癌转移风险有一定指导意义,ｎｍ２３－Ｈ１与乳腺癌淋巴结转移无明显相关性     

乳腺癌组织中CD44v6,C—erbB—2的表达及其意义

该文利用免疫组化法对６例乳腺癌患者进行ＣＤ４４ｖ６，Ｃ－ＥＲｂＢ－２检测。结果发现：ＣＤ４４ｖ６的阳性表达与乳腺癌的腋窝淋巴结转移及与Ｃ－ｅｒｂＢ－２的阳性表达密切相关（Ｐ＜０．０１）。提示ＣＤ４４ｖ６可作为乳腺癌淋巴结转移的预测指标。     

乳腺癌细胞rasP21基因表达水平检测的临床意义

应用免疫组化方法检测了３７例乳腺癌２５例良性乳腺疾病和１０例正常乳腺组织ｒａｓ癌基因产物Ｐ２１的表达水平，结果显示：乳腺癌Ｐ２１的阳性反应率（７０．３％）远高于良性乳腺疾病（８％）及正常乳腺（０％）且Ｐ２１表达水平随肿瘤体积，局部浸润和淋巴结转转移的扩展而升高（Ｐ〈０．０１），癌组织分化低者Ｐ２１表达水平较高（Ｐ〈０．０５），提示Ｐ２１过度表达是乳腺癌预后不良的征象。     

喉癌患者多药耐药基因、多药耐药相关蛋白基因的表达及临床意义

应用逆转录－多聚酶链式反应（ＲＴ－ＰＣＲ）技术,检测了３５ 例喉癌患者ＭＤＲ－１ ｍ ＲＮＡ、ＭＲＰｍ ＲＮＡ 基因的表达,同时,应用免疫组化ＳＡＢＣ法检测了６１ 例患者ＭＤＲ－１ 基因产物Ｐ－ｇｐ。喉癌组织中ＭＤＲ－１ ｍ ＲＮＡ 及Ｐ－ｇｐ 的阳性表达率分别为３７．１％ （１３／３５）和３４．４％ （２１／６１）;３５ 例ＲＴ－ＰＣＲ 检测标本中,两项指标间有一定的相关性（Ｐ＜ ０．０１）。晚期患者（Ｔ３－ ４,１８／４０）Ｐ－ｇｐ 阳性表达率明显高于早期患者（Ｔ１－ ２,３／２１,Ｐ＜ ０．０５）。喉癌组织中ＭＲＰｍ ＲＮＡ阳性表达率为４５．７％ （１６／３５）,其阳性表达强度在晚期肿瘤中（１４／２３）明显增高（Ｐ＜ ０．０５）,且与肿瘤颈淋巴结转移（Ｎ０,８／２７;Ｎ１－ ２,８／８）呈明显相关性（Ｐ＜ ０．０１）     

P－选择素在胃癌中表达及其预后意义

应用免疫组化ＬＳＡＢ法对６０例胃癌患者检测Ｐ－选择素在胃癌组织中的表达，并对其中的５５例作长期随访，以研究与胃癌组织学分型、分化程度、淋巴转移、ＴＮＭ分期及其与；临床预后的关系。结果发现６０例胃癌组织中３２例阳性（阳性率５３．３％）。Ｐ－选择素不仅在肿瘤血管内皮上表达，也在肿瘤细胞膜上表达。淋巴结有癌转移者Ｐ－选择素阳性表达率为７０．３％（２６／３７例），显著高于无淋巴结转移的２６．１％（６／２３）（Ｐ＜０．０１）；晚期胃癌（Ⅲ、Ⅳ期）阳性率为８０％，显著高于Ⅰ、Ⅱ期早期胃癌２６．７％的表达率（Ｐ＜０．０１）。５５例随访资料分析表明，Ｐ－选择素阳性表达者平均生存期（２５．５±２１．８月）和五年生存率（１０．７％）显著低于阴性者（Ｐ＜０．０１）。研究结果提示Ｐ－选择素与胃癌浸润转移相关，可能是一种新的胃癌预后指标。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.