变应性鼻炎患者鼻腔灌洗液中细胞间黏附分子-1和白细胞介素-6水平及其与气道反应性的关系

OBJECTIVE: To investigate the relation between levels of intercellular adhesion molecule-1, interleukin-6 and airway hyperresponsiveness in patients with allergic rhinitis. METHODS: Fifty-four patients with allergic rhinitis (AR) and 20 controls were included in the study. The levels of intercellular adhesion molecule-1 (ICAM-1) and interleukin-6 (IL-6) in nasal lavage fluid, gathered 1 hour after specific allergen nasal provocation test (SANPT), were detected by sandwich enzyme-linked immunosorbent assay (ELISA) technique. The pulmonary function (FEV1) and nonspecific bronchial provocation test were measured in 54 patients with AR, 36 patients with AR and bronchial asthma (BA) and 20 controls. At the same time, the correlation between levels of ICAM-1 and IL-6 in nasal lavage fluid and pulmonary function (FEV1) was studied. RESULTS: The levels of ICAM-1 and IL-6 in nasal lavage fluid from patients with AR were (272.75 +/- 32.25) pg/ml and (52.11 +/- 16.54) pg/ml, significantly higher than those the controls, which were (158.82 +/- 33.88) pg/ml and (25.64 +/- 10.14) pg/ml (P < 0.01). The pulmonary function (FEV1) in patients with AR and BA was (78.82 +/- 7.41)%. It was obviously lower than that in patients with AR [(83.90 +/- 4.87)%], much lower than that in normal controls [(90.25 +/- 4.69)%]. The difference among them was significant. In patients with AR, the positive percentage of bronchial provocation test was 64.81%, in patients with AR and BA, it was 83.33% in normal controls, it was 0. The differences among them had very significant meaning. The levels of ICAM-1 and IL-6 in nasal provocation fluid had closely negative correlation with pulmonary function (FEV1), r = -0.7071, -0.6248, P < 0.01. CONCLUSIONS: The close correlation was observed in upper and lower airway for allergic inflammation. The pulmonary function of patients with AR was lower, and 64. 8% of them had airway hyperresponsiveness, so that they had the potent possibility to have bronchial asthma.     

变应性鼻炎患者鼻腔灌洗液中细胞间黏附分子-1和白细胞介素-6水平及其与气道反应性的关系

目的 探讨变应性鼻炎患者鼻腔灌洗液中细胞间黏附分子-1(intercellular adhesionmolecule-1,ICAM-1)和白细胞介素-6(interleukin-6,IL-6)与气道反应性的关系。方法 采用双抗体夹心酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测54例变应性鼻炎患者和20例健康对照,在特异性变应原鼻激发试验(specific allergen nasal provocation test,SANPT)后1h的鼻腔灌洗液中ICAM-1和IL-6的浓度。对54例变应性鼻炎患者和36例鼻炎合并支气管哮喘(缓解期)患者及20例健康对照进行肺功能检查和非特异性乙酰甲胆碱支气管激发试验,并采用直线相关分析方法分析其鼻腔灌洗液中ICAM-1、IL-6水平与肺功能第1秒用力呼气率(FEV1)的相关性。结果 变应性鼻炎患者鼻腔灌洗液中ICAM-1、IL-6的浓度分别为(272.75±32.25)pg/ml、(52.11±16.54)pg/ml,均明显高于健康对照组的(158.82±32.88)pg/ml和(25.64±10.14)pg/ml,差异有极显著意义(P均<0.01)。变应性鼻炎患者肺功能FEV1为(83.90±4.87)％,鼻炎合并哮喘患者为(78.82±7.41)％,健康对照为(90.25±4.69)％,三组间差异均有极显著意义(P均<0.01)。变应性鼻炎患者支气管激发试验阳性率为64.8％,鼻炎合并哮喘患者为83.33％,健康对照组无阳性,三组间差异均有极显著意义(     

转化生长因子β1白细胞介素-6及11和17在变应性鼻炎患者鼻黏膜中的表达

目的：探讨转化生长因子β1（TGF-β1），白细胞介素（IL）-6、11和17在变应性鼻炎患者鼻黏膜中的表达及意义。方法：采用酶联免疫吸附试验定量检测19例变应性鼻炎患者（变应性鼻炎组）下鼻甲黏膜和21例行鼻中隔偏曲矫正术的非变应性鼻炎患者（对照组）下鼻甲黏膜组织中TGF-β1，IL-6、11和17的蛋白表达量。结果：①变应性鼻炎组的TGF-β1蛋白表达量显著高于对照组（P〈0．01）；②IL-6、11和17的蛋白表达量在两组间差异无统计学意义（均P〉0．05）；③TGF-β1，IL-6、11和17的蛋白表达量在并发哮喘和不并发哮喘的变应性鼻炎患者间的表达差异无统计学意义（均P〉0．05）。结论：TGF-β1可能在变应性鼻炎的组织结构重塑中起作用，而IL-6、11和17可能在此过程中无明显作用，这可能是变应性鼻炎和哮喘组织结构重塑差异的分子基础之一。     

白细胞介素-18在变应性鼻炎中的作用

目的 :探讨白细胞介素 18(IL 18)在变应性鼻炎发生发展中的作用。方法 :采用双抗体夹心ELISA法检测 30例变应性鼻炎患者 (鼻炎组 )和 2 0例健康志愿者 (对照组 )血清中IL 18的含量。结果 :鼻炎组血清IL 18含量 [(4 2 4 .12± 6 0 .4 7)ng/L]高于对照组 [(2 10 .71± 5 7.37)ng/L],其差异有统计学意义 (P <0 .0 1) ;变应性鼻炎伴哮喘者 [(5 91.19± 4 6 .5 4 )ng/L]高于不伴哮喘者 [(35 2 .5 2± 6 4 .2 5 )ng/L],其差异亦有统计学意义 (P <0 .0 5 )。结论 :变应性鼻炎患者血清IL 18表达增高 ,在变应性炎症的持续存在和恶化过程中起重要作用。     

细胞黏附分子及一氧化氮合酶在变应性鼻炎鼻黏膜中的表达

目的:对细胞黏附分子(CAM)及一氧化氮合酶(NOS)进行原位观察,探讨它们在变应性鼻炎(AR)发病中的作用。方法:采用链霉卵白素-生物素复合体(SABC)法,对AR患者及对照组手术切除的下鼻甲黏膜内细胞间黏附分子-1(ICAM-1)、血管CAM-1(VCAM-1)和淋巴细胞功能相关抗原-1(LFA-1),以及神经型NOS(nNOS)、诱导型NOS(iNOS)和内皮细胞型NOS(eNOS)进行原位检测。结果:AR下鼻甲黏膜内3种CAM表达的阳性细胞数ICAM-1为[(14.4±2.2)个/HP(×400),以下同],LFA-1为(17.2±3.3)个/HP,VCAM-1为(11.5±2.7)个/HP;对照组下鼻甲黏膜内3种CAM表达的阳性细胞数ICAM-1为(8.7±1.8)个/HP,LFA-1为(10.3±2.1)个/HP,VCAM-1为(6.9±1.8)个/HP。t值分别是11.57,10.02和8.07(均P<0.01)。AR及对照组下鼻甲黏膜内nNOS表达的阳性细胞数分别为(9.4±1.7)个/HP和(4.7±1.3)个/HP,t值为12.62,(P<0.01);iNOS表达的阳性细胞数分别为(27.5±3.2)个/HP和(4.3±1.7)个/HP,t值为36.03(P<0.01)。eNOS表达的阳性细胞数分别为(6.5±2.1)个/HP,(6.2±1.9)个/HP,t值为0.62(P>0.05)。结论:CAM在黏膜上皮、腺上皮、血管内皮以及黏膜下的各种炎性细胞等的表达,说明CAM参与AR的发生、发展。nNOS和iNOS在AR的发病过程中可能起重要作用。     

白细胞介素23在变应性鼻炎患者鼻黏膜中的表达及意义

目的:探讨白细胞介素23(IL-23)在变应性鼻炎(AR)患者鼻黏膜中的表达及意义.方法:收集12例AR患者(AR组)下鼻甲黏膜及11例单纯性鼻中隔偏曲患者下鼻甲黏膜(对照组),采用免疫组织化学方法和实时定量RT-PCR方法检测下鼻甲黏膜中IL-23的表达.结果:IL-23p19 mRNA在AR组中的表达水平较对照组明显增高(P<0.01).免疫组织化学显示IL-23蛋白主要表达于黏膜固有层的炎性浸润细胞,AR组鼻黏膜中IL-23阳性细咆数明显高于对照组(P<0.01).IL-23p19mRNA和蛋白表达水平与黏膜固有层总炎性细胞浸润程度及固有层胶原纤沉积程度成显著正相关(对于mRNA表达水平,相关系数分别为0.678和0.834;对于蛋白表达水平,相关系数分别0.644和0.721,均P<0.01).结论:IL-23可能参与了AR的慢性炎症和组织重塑.     

黏附分子在实验性变应性鼻炎鼻黏膜中的表达

变应性鼻炎迟发相反应阶段是以嗜酸粒细胞为代表的大量的炎性细胞的浸润而导致的慢性炎症，血管细胞黏附分子-1(vascular cell adhesion molecule-1，VCAM-1)、细胞间黏附分子-1(intercellular adhesion molecule-1，ICAM-1)在嗜酸粒细胞等炎性细胞的黏附、浸润到黏膜炎症区域起着重要作用。本研究观察实验性变应性鼻炎鼻黏膜中黏附分子的表达。     

白细胞介素-12在变应性鼻炎鼻粘膜中的表达

目的 :探讨白细胞介素 12 (IL 12 )在变应性鼻炎鼻粘膜中的表达。方法 :以卵清蛋白为变应原建立豚鼠变应性鼻炎模型 (模型组 )。取该模型和健康豚鼠鼻粘膜行常规HE染色 (对照组 ) ,并采用逆转录 聚合酶链反应 (RT PCR)检测方法 ,对两组动物鼻粘膜组织中IL 12mRNA表达水平进行相对定量比较。结果 :IL 12mR NA在两组鼻粘膜中均有表达 ,模型组的表达水平为 0 .6 6 7± 0 .10 4显著低于正常对照组 0 .84 7± 0 .0 71(P <0 .0 1)。结论 :在变应性鼻炎鼻粘膜组织中IL 12的表达下降 ,提示应用IL 12替代疗法治疗变应性鼻炎的可能性。     

白细胞介素-5和白细胞介素-16在变应性鼻炎患者血清中的表达及临床意义

目的　探讨白细胞介素-5（interleukin-5,IL-5）和白细胞介素-16（interleukin-16,IL-16）在变应性鼻炎（allergic rhinitis,AR）患者血清中的表达及临床意义。方法　采用酶联免疫吸附试验检测64例持续性AR患者及20例正常对照组血清中IL-5、IL-16水平,并分析与AR之间的相关性。结果　与对照组相比,持续性AR患者血清中IL-5、IL-16均明显升高（P <0.05）。持续性中重度组患者血清中IL-5、IL-16水平均明显高于持续性轻度组,二者比较差异有统计学意义（P <0.05）。持续性AR患者血清中的IL-5与IL-16水平呈正相关（r =0.566,P <0.05）。结论　IL-5、IL-16参与变应性鼻炎的发生发展,二者在AR的临床诊断、 治疗中具有广阔应用前景;IL-16具有较高特异性,对AR患者早期诊断具有重要临床意义。     

变应性鼻炎患者白细胞介素-4和白细胞介素-8的研究

变应性鼻炎在临床变态反应学科中属常见病、多发病。许多研究证实，细胞因子在变态反应性疾病的发生与发展中起着重要的作用。为探讨白细胞介素-4（IL－4）和白细胞介素一8（IL－8）在变应性鼻炎患者的发生与发展中的作用，我们检测了IL－4和IL－8在变应性鼻炎患者中的水平，以对细胞因子在变应性鼻炎发病中的作用机理进行初步的探讨，报道如下。1对象与方法1．1观察对象变应性鼻炎组：25例，均符合1991年《中华耳鼻咽喉科杂志》中的诊断标准。男15例，女10例；年龄（35．2±612）岁。鼻炎史1～30年不等，有家族过敏史考18例，个人过敏…     

Localization of intercellular adhesion molecule-1 in middle ear cholesteatoma

Intercellular adhesion molecule-1 (ICAM-1) may have a role in acquired cholesteatoma, which is usually associated with an inflammatory reaction occurring in the middle ear cavity. The presence of ICAM-1 in human cholesteatomas was demonstrated by an immunoblotting assay using a specific monoclonal anti-ICAM-1 antibody after protein extraction. Distribution of ICAM-1 in the cholesteatoma tissues was then studied by avidin-biotin-peroxidase complex staining. ICAM-1 appeared to be localized on keratinocytes in all layers of the epithelium and on Langerhans cells in both the epithelium and granulation tissue of cholesteatoma. ICAM-1 was not found in the epidermis of normal external ear canal skin, normal tympanic membrane or normal facial skin, but significant staining was seen on keratinocytes of hair follicles and glands in the facial skin. The present study is the first to demonstrate ICAM-1 in cholesteatoma and suggests that it may have an important role in the clinical development of cholesteatoma, including migration, adhesion and proliferation of lymphocytes, Langerhans cells and keratinocytes.     

豚鼠实验性变应性鼻炎最轻持续炎性反应模型的建立

目的 建立豚鼠实验性变应性鼻炎最轻持续炎性反应(minimal persistent inflammation,MPI)模型,并初步探讨其病理机制及意义.方法 60只健康雄性Hartley系豚鼠,随机分为A～D共4组,每组15只.A组为阳性对照组,B组为实验组即MPI组,C组为阴性对照组,D组为空白对照组.首先以含卵清蛋白和氢氧化铝凝胶的生理盐水混合液对A、B、C 3组行基础致敏和强化致敏,再分别用1%和0.01%的卵清蛋白溶液或生理盐水长期鼻腔激发.D组始终以生理盐水进行致敏和激发.观察豚鼠鼻腔激发后症状(喷嚏)变化并检测鼻黏膜中嗜酸粒细胞(eosinophils,EOS)浸润程度及上皮细胞内细胞间黏附分子1(intercellular adhesion molecule 1,ICAM-1)的表达情况.结果 MPI模型组在以l%卵清蛋白溶液激发时,喷嚏平均次数明显多于D组(P<0.05),而与A、C组相比差异无统计学意义(P值均>O.05);改用0.01%卵清蛋白溶液鼻腔激发后,变应性鼻炎症状基本消失,与D组相比差异无统计学意义(P>0.05),但鼻黏膜内仍有少量EOS浸润,EOS计数明显多于D组(P相似文献   

细胞粘附分子在变应性鼻炎鼻甲粘膜中的表达及其意义

目的 :探讨变应性鼻炎鼻甲粘膜组织中细胞粘附分子的表达及其意义。方法 :对 2 0例慢性肥厚性鼻炎、2 4例变应性鼻炎非发作期和 2 0例变应性鼻炎发作期患者下鼻甲粘膜组织石蜡切片 ,用细胞间粘附分子 1(intercellularadhesionmolecule 1,ICAM 1)和淋巴细胞功能相关抗原 1(lymphocytefunction associatedantigen 1,LFA 1)单抗进行免疫组织化学染色 ,光镜观察。结果 :与变应性鼻炎非发作期和慢性肥厚性鼻炎相比 ,变应性鼻炎发作期鼻甲组织ICAM 1和LFA 1的表达增高 (P <0 .0 1)。结论 :变应性鼻炎发作期鼻甲粘膜组织中ICAM 1和LFA 1呈高表达 ,其参与了变应性鼻炎的炎症过程。细胞间粘附分子 (ICAM 1 LFA 1)是变应性鼻炎发生、发展的重要因素之一。     

重组激活基因-1在变应性鼻炎鼻黏膜中的表达及DNA测序研究

目的:探讨重组激活基因1(RAG1)在变应性鼻炎(AR)发病机制中的作用及其与IL4、IL10和IgE之间的关系及意义。方法:用卵清蛋白(OV)建立大鼠AR模型并用地塞米松(DEX)进行干预,用被动皮肤过敏原试验(PCA)测试皮肤反应并与正常对照组进行比较;用RTPCR技术检测各组鼻黏膜中RAG1基因mRNA的表达水平,并对RAG1基因的DNA进行测序;用ELISA法检测各组大鼠外周血清IL4、IL10和IgE的水平变化。结果:RAG1基因的mRNA在正常对照组鼻黏膜中未见表达,在AR组和DEX干预组均有明显表达;DNA测序未发现RAG1基因有突变发生;AR组血清IL4[(106.31±12.90)ng/L]和IgE[(38.67±4.13)ng/L]水平均显著高于正常对照组[(93.65±7.78)ng/L,(23.27±1.36)ng/L](均P<0.05),IL10[(38.15±4.89)ng/L]水平显著低于正常对照组[(48.74±3.49)ng/L](P<0.05);经DEX干预后,血清IL4[(92.67±16.40)ng/L]和IgE[(24.23±4.38)ng/L]水平均较AR组显著下降(均P<0.05),而IL10[(46.18±5.01)ng/L]水平较AR组显著升高(P<0.05);PCA试验在AR组90%呈阳性反应,在正常对照组和DEX干预组均呈阴性反应。结论:RAG1的高表达与AR的发病密切相关,并介导了IL4和IgE的分泌及抑制IL10的分泌。其DNA序列在AR鼻黏膜中具有保守性,糖皮质激素不影响RAG1基因的表达,推测在AR发病机制中,RAG1基因通过一个较高层面的免疫调节环节发生作用。     

白介素-5在变应性鼻炎鼻黏膜中的表达及意义

目的：了解白介素 5（IL 5）在变应性鼻炎鼻粘膜内浸润的炎性细胞中的表达及分布,探讨IL 5与嗜酸性粒细胞积聚及发病机制的关系。方法：采用免疫组化染色法（SP法）对变应性鼻炎下鼻甲粘膜（A组）、单发鼻息肉组织（B组）、无变应性鼻炎下鼻甲粘膜组织（C组）切片进行IL 5染色,对IL 5染色阳性细胞计数分类,对统计结果行方差分析。结果：A、B两组组织中可见较多的IL 5阳性染色细胞,多见嗜酸性细胞、淋巴细胞、中性粒细胞等阳性染色,两组IL 5阳性细胞总数、Eos计数无统计学差异,但均高于C组（P＜0.01）。结论：IL 5在变应性鼻炎鼻粘膜、鼻息肉组织内浸润的多种炎性细胞中表达,能客观反映免疫或炎症反应的程度,可作为变应性鼻炎诊断评分系统的补充。     

Nasal interleukin-5, immunoglobulin E, eosinophilic cationic protein, and soluble intercellular adhesion molecule-1 in chronic sinusitis, allergic rhinitis, and nasal polyposis

OBJECTIVE: To compare concentrations of interleukin-5 (IL-5), immunoglobulin E (IgE), eosinophilic cationic protein (ECP), and soluble intercellular adhesion molecule-1 (sICAM-1) in nasal secretion and serum of patients with chronic nonallergic sinusitis, allergic rhinitis, and nonallergic nasal polyposis to obtain information about the pathogenesis of these diseases. METHODS: Nasal secretion and serum were analyzed by routine enzyme-linked immunosorbent assay techniques. Nineteen patients with chronic nonallergic sinusitis, 24 patients with seasonal allergic rhinitis, and 18 patients with nonallergic nasal polyposis were included in the study. Eight healthy, nonallergic probands served as control subjects. RESULTS: Significantly elevated concentrations of IL-5 (5-fold, P < .05) and IgE (15-fold, P < .01) were detected in nasal secretion of patients with allergic rhinitis (IL-5, 51.8 +/- 13.2 pg/mL; IgE, 41.9 +/- 20.9 kU/L) or nonallergic nasal polyposis (IL-5, 57.9 +/- 36.9 pg(mL; IgE, 40.5 +/- 20.2 kU/L) compared with controls (IL-5, 10.6 +/- 7.8 pg/mL; IgE, 2.8 +/- 0.5 kU/L) or with patients with chronic nonallergic sinusitis (IL-5, 16.5 +/- 13.2 pg/mL; IgE, 5.4 +/- 3.1 kU/L). There were no significant differences between patients with allergic rhinitis and those with nonallergic nasal polyposis. Concentrations of ECP were significantly elevated (sixfold, P < .01) in patients with allergic rhinitis (297.8 ng/mL +/- 173.1) compared with controls (52.4 +/- 28.0 ng/mL) or patients with chronic nonallergic sinusitis (44.8 +/- 40.1 ng/mL), whereas twofold higher concentrations (not significant) of ECP were observed in patients with nonallergic nasal polyposis (107.1 +/- 26.6 ng/mL). Significantly elevated concentrations of sICAM-1 in nasal secretion (threefold, P < .05) were detected only in patients with chronic nonallergic sinusitis (79.4 +/- 45.6 ng/mL). The elevated sICAM-1 nasal secretion values in this group correlated significantly (P < .05) to the serum values. CONCLUSIONS: Equally elevated concentrations of IL-5 and IgE in patients with allergic rhinitis and nonallergic nasal polyposis implicated similar pathogenic processes in both diseases. Whereas the pathogenesis of allergic rhinitis is IgE-specific, the pathogenesis of nasal polyps is not as clear. IL-5 was suggested to play a pivotal role in tissue eosinophilia, which was confirmed by data in the present study. Elevated concentrations of ECP were suggested to result from tissue eosinophilia--a characteristic of both diseases. Elevated concentrations of sICAM-1 in patients with chronic nonallergic sinusitis pointed to its key role in the recruitment of neutrophils into the inflamed tissue, whereas an important role in eosinophil recruitment was ruled out.     

鼻息肉患者血清中可溶性细胞间黏附分子-1的水平及临床意义

目的:探讨可溶性细胞间黏附分子-1(sICAM-1)在鼻息肉发病中的作用及糖皮质激素治疗鼻息肉的机制。方法:采用双抗体夹心酶联免疫吸附法(ELISA)测定鼻息肉患者经糖皮质激素全身和局部治疗前、后血清中sICAM-1的含量变化,同时观察糖皮质激素对鼻息肉的治疗效果。结果:①鼻息肉患者血清中sICAM-1的含量高于正常对照组;②经糖皮质激素治疗后,鼻息肉患者血清中sICAM-1含量显著下降,显效组sICAM-1下降的程度大于有效组;③糖皮质激素治疗前、后,患者鼻息肉大小计分减少程度与血清中sICAM-1下降程度成正相关。结论:细胞间黏附分子-1(ICAM-1)在鼻息肉发生发展过程中起着重要作用。糖皮质激素可以通过对抗炎性细胞间黏附而对鼻息肉起治疗作用,血清中sICAM-1的水平变化可作为鼻息肉药物治疗疗效的临床判断指标。