彝药海枫藤的生药学鉴定

目的 对彝药海枫藤进行了生药学研究,为鉴别海枫藤提供生药学资料,为该药材的深入研究和开发利用提供鉴别依据.方法 采用原植物鉴别、性状鉴别、显微鉴别、理化鉴别的方法.结果 得出主要性状、显微鉴别和理化鉴别的特征.结论 通过原植物鉴别、性状鉴别、显微鉴别、理化鉴别能够较好地鉴定和利用该药材.     

蒙药麝香的生药学研究

本文对蒙药麝香进行了基源鉴别,性状鉴别、显微鉴别、理化鉴别和经验鉴别等方面的研究,为该药材的使用提供鉴别依据。     

动物药材鉴别方法研究的概况及展望

本文对动物药材的传统鉴别、显微鉴别、理化鉴别,尤其是理化鉴别中的紫外光谱鉴别、红外光谱鉴别、电泳鉴别和免疫鉴别等方法进行了概述和展望。     

锦灯笼的生药鉴别研究

锦灯笼药材为各版中国药典一部所收载的药材,本实验针对锦灯笼的基源鉴别、性状鉴别、显微鉴别、紫外吸收光谱鉴别、薄层色谱鉴别等进行了研究。为更好的生药鉴别、开发利用奠定了基础。     

番红花的真伪鉴别

从性状鉴别、理化鉴别、显微鉴别等方面对正品番红花、番红花的常见伪品和掺伪品进行鉴别研究,为准确鉴别番红花提供理论依据。     

试述中药经验鉴别法

中药鉴别的方法有很多,最常用的有来源鉴别、性状鉴别、理化鉴别、显微鉴别、遗传分子鉴别等。而日常工作中用的最多的就是性状鉴别法,即用眼观、手摸、鼻闻、口尝以及水、火等简单的方法来鉴别中药材的外观性状,通常称为经验鉴别法。     

檀香叶的鉴别研究

目的对檀香叶进行鉴别研究。方法按常规生药学研究方法进行性状鉴别、显微鉴别、薄层色谱鉴别。结果详细描述了檀香叶的各项鉴别特征,确立了其主要鉴别点。结论为檀香叶的鉴别及资源开发利用提供了科学依据。     

冬虫夏草真伪鉴别方法研究进展

冬虫夏草为传统的名贵中药材,近年来由于疯狂的采挖使得其资源急剧减少,濒临灭绝。目前市场上冬虫夏草价格堪比黄金,掺伪现象严重。本文从性状鉴别、显微鉴别、分子生物学鉴别和化学鉴别（理化鉴别、光谱鉴别、色谱鉴别和色谱-光谱联用鉴别）四个方面,对近10年来国内外冬虫夏草真伪鉴别方法研究文献进行了概括和总结,为市场上冬虫夏草的真伪鉴别提供科学参考依据。     

3种易混淆中药材鉴别技术进展分析

笔者通过查阅近十年的研究文献,对含有马兜铃酸类成分的三种易混淆中药材的鉴别技术的研究进展进行总结与分析,木通为关木通的替代品,混淆品有多种,目前已经有性状鉴别、显微鉴别、薄层鉴别、光谱鉴别、DNA分子鉴别等方法对木通、关木通以及川木通进行鉴别;在广防己、防己以及其混淆品的鉴别上,已经有性状鉴别、紫外光谱鉴别以及DNA条形码鉴别等方法;在青木香及其混淆品的鉴别上,已经有性状鉴别、显微鉴别、HPLC指纹图谱以及UPLC指纹图谱等方法。     

蒙药材并头黄芩生药鉴别研究

目的:为蒙药材并头黄芩的性状及显微鉴别、薄层鉴别提供相关依据.方法:对不同产地蒙药材并头黄芩进行性状鉴别、显微鉴别、薄层色谱鉴别.结果:建立并头黄芩的性状、粉末的显微特征;薄层鉴别中,薄层图谱清晰,与相邻斑点分离较好,阴性对照无干扰所建立的方法简便、可靠、重复性好、专属性强.结论:建立的蒙药材并头黄芩性状鉴别、显微鉴别...     

蛇床子乳剂的制备及体外抗阴道滴虫考察

目的:将蛇床子中的总香豆素制成o/w型乳剂,并考察其体外抗阴道滴虫药理活性。方法:采用超声法提取蛇床子中的总香豆素,选取乳化时间、乳化次数、乳化压力及乳化剂加入顺序为考察因素,通过单因素试验优选蛇床子乳剂的制备工艺,并考察其体外抗阴道滴虫药效学试验。结果:确定的蛇床子乳剂制备工艺为乳化时间15 min,乳化压力150 MPa,乳化3次,乳化剂加入顺序为油相加入到水相;确认其对临床分离的阴道滴虫有较好的抑制作用。结论:该制备工艺稳定可行,制备的乳剂对阴道滴虫产生抑制作用的最低药物质量浓度0.15～0.62 g·L-1。     

Qualitative Analysis and Quality Evaluation of Cnidium monnieri Using UHPLC-ESI-Q-TOF/MS

Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on C. monnieri. Methods Qualitative analysis of coumarins in C. monnieri was detected by UHPLC-ESI-Q-TOF/MS. Quadrupole TOF/MS in either full scan mode or extracted ion mode was used for the qualitative analysis of the constituents. Relative peak area of each component was used for the hierarchical cluster analysis. Results According to UHPLC-ESI-QTOF-MS data, chemical structures of 28 coumarins in the fruits of C. monnieri were identified, including 19 simple coumarins, seven linear coumarins, and two angular coumarins. Among these constituents, ten coumarins were firstly identified in C. monnieri. In addition, hierarchical cluster analysis suggested that C. monnieri from different regions could be classified into four groups, and this clustering was correlated to the distribution significantly, Xuzhou could be regarded as the genuine producing area. Conclusion UHPLC-ESI-Q-TOF-MS is a viable method for qualitative analysis and quality evaluation of coumarins from the fruit of C. monnieri. Coumarins in C. monnieri exists intra-species variance, which indicates significant meaning for the quality control and choice of famous region drug for C. monnieri in the clinic medication.     

Studies on the quality of fructus Cnidii--comparison of antibacterial action]

Comparative studies on antibacterial and antifungal action of the fruits of C. monnieri from four habitats and two other species of Cnidium showed they had inhibitory potency to some bacterial and fungi in vitro. C. monnieri and C. salium were more effective than C. dahuricum in antibacterial activities. C. monnieri had higher antifungal effect than C. salium and C. dahuricum.     

蛇床及其近缘物种的ITS2分子鉴定

目的：对传统中药蛇床子基原植物及其近缘物种的ITS2条形码序列进行分析,探讨药用植物蛇床子的分子鉴定方法。方法：采用PCR技术扩增蛇床ITS2基因片段,进行双向测序,运用CodonCodeAligner拼接后,用MEGA5．0软件进行相关数据分析,构建NJ树。应用Schuhz等建立的ITS2数据库及其网站预测ITS2二级结构。结果：蛇床与其近缘物种ITS2序列间存在明显差异,基于ITS2序列的NJ树及其ITS2二级结构均可以将蛇床及其近缘物种区分开。结论：ITS2可有效地鉴别蛇床及其近缘物种。     

蛇床种内变异的RAPD分析

目的 :从DNA分子水平上探讨不同地区产蛇床的种内变异。方法 :用随机扩增多态性DNA(RAPD)技术分析 9个产地的蛇床基因组DNA多态性 ,并构建树状聚类图。结果 :从 2 0个 10bp随机引物中筛选出 12个有效引物 ,共扩增出 75条DNA带 ,其中 6 4条表现出多态性。结论 :地理分布距离越小 ,蛇床的遗传差异越小 ,反之越大 ,但生态环境对蛇床遗传变异的作用也不可忽视。     

蛇床子的体视学研究和计算机图像分析测定

经对4种蛇床的果实、花粉粒、维管束、气孔和内胚乳细胞的大小进行分析测定,结果表明,果实和维管束的大小顺序为兴安蛇床>碱蛇床>滨蛇床>蛇床:花粉粒大小为碱蛇床>兴安蛇床>滨蛇床>蛇床;内胚乳细胞则兴安蛇床>滨蛇床>碱蛇床>蛇床。     

Antitumor effects of Osthol from Cnidium monnieri: an in vitro and in vivo study

Cnidium monnieri (L.) Cusson is a Chinese medicine which is used widely by traditional medicine doctors. Osthol is a major bio-activity compound of the herb. In this study, osthol was isolated from C. monnieri and its in vitro and in vivo antitumor effects studied. The results of the in vitro study showed: that osthol inhibited the growth of HeLa, in a time- and concentration-dependent manner, with IC(50) values of 77.96 and 64.94 microm for 24 and 48 h, respectively; that osthol had lower cytotoxic effects in primary cultured normal cervical fibroblasts; and that increased DNA fragmentation and activated PARP in HeLa after treatment with osthol which could induce apoptosis. The results of the in vivo model showed that the survival days of the P-388 D1 tumor-bearing CDF(1) mice were prolonged (ILS% = 37) after osthol (30 mg/kg) was given once a day for 9 days. Based on these results, it is suggested that osthol could inhibit P-388 D1 cells in vivo and induce apoptosis in HeLa cells in vitro, and that osthol is good lead compound for developing antitumor drugs. However, C. formosanum Yabe of Taiwan's endemic plants contained little osthol, with no imperatorin, and its major components were different from that of C. monnieri. Therefore, it is suggested that C. formosanum also may possess economic worth.     

蛇床子地理分布与叶绿体matK基因序列的相关性分析

 目的探讨中药蛇床子 Cnidium monnieri(L.)Cuss.居群间的DNA序列变异与地理分布的关系,为蛇床子品质评价提供分子依据。方法采用PCR直接测序技术对蛇床子6个居群6个个体样品的叶绿体matK基因进行测序分析研究。结果蛇床子6个居群的matK基因核苷酸序列长1268 bp,编码422个氨基酸成熟酶。根据排序比较,蛇床子6个居群间的matK基因序列存在12个变异位点,氨基酸序列10个变异位点。邻接法构建的系统分支树表明：蛇床子居群间的亲缘关系与地理分布及所含香豆素化学型呈良好的相关性。结论结合香豆素分析数据,基因测序分析技术可以成为蛇床子品质评价的有力工具。     

6种郁金类药用植物的同工酶研究

目的：探讨姜黄属Curcuma 6种郁金类药用植物39份材料间的亲缘关系。方法：采用聚丙烯酰胺凝胶电泳技术(PAGE)，作过氧化物酶(POD)和酯酶(EST)同工酶分析，并用NTSYSpc 2.1软件对谱带作聚类分析。结果：在EST同工酶分析中，蓬莪术C. phaeocaulis、温郁金C. wenyujin、广西莪术C. kwangsiensis、川黄姜C. chuanhuangjiang有它们自己的特征谱带，而川郁金C. sichuanensis和姜黄C. longa有着共同的特征谱带；在POD同工酶分析中，只有蓬莪术和广西莪术有自己的特征谱带。结论：同种植物不同材料间亲缘关系的远近与地理来源关系不大；姜黄和川郁金亲缘关系很近，不能通过同工酶将它们区别开来。     

Wound healing and protease inhibition activity of Bacoside‐A,isolated from Bacopa monnieri wettest

Bacopa monnieri (L.) Wettest. (Scrophulariaceae) is a well‐known medicinal herb. In the Indian system of medicine it is known as Brahmi (Sanskrit) and Indian water hyssop. Methanolic extract of Bacopa monnieri and its isolated constituent Bacoside‐A were screened for wound healing activity. Bacoside‐A was screened for wound healing activity by excision, incision and dead space wound on Swiss albino rats. Significant wound healing activity was observed in both extract and the Bacoside‐A treated groups. The SDS‐PAGE caseinolytic zymogram analysis of inhibition of matrix metalloproteases (MMPs) enzyme from the excision wound by Bacoside‐A, an isolated constituent, was done with the concentrations 100 and 200 μmg/ml. In Bacoside‐A treated groups, epithelialization of the excision wound was faster with a high rate (18.30 ± 0.01 days) of wound contraction. The tensile strength of the incision wound was increased (538.47 ± 0.14 g) in the Bacoside‐A treated group. In the dead space wound model, the weight of the granuloma was also increased (89.15 ± 0.08 g). The histological examination of the granuloma tissue of the Bacoside‐A treated group showed increased cross‐linking of collagen fibers and absence of monocytes. The wound healing activity of Bacoside‐A was more effective in various wound models compared to the standard skin ointment Nitrofurazone. Copyright © 2010 John Wiley & Sons, Ltd.