Effects of aluminum, fluorine and their mixtures on the enzyme activities of jejunal mucus membrane in the perfusion of small intestine of rats in vivo]

Buffer (control), F as NaF (100 mmol/L), Al as AlCl3 (32 and 170 mmol/L) and the two mixtures of Al and F (Al:F = 0.3:0.7 and 0.7:0.3) in Tris-buffered media of pH 7.0 were respectively perfused through the small intestine of rats over a period of 40 min. The jejunum was taken out from each animal immediately. Some of its parts were for HE staining; and the others for enzyme histochemical staining. Fifteen enzymes were observed. The results showed that (1) Fluorine chiefly inhibited the metal-enzymes and metal-activated enzymes; aluminum mainly depressed the activities of enzymes in relation to the cell energy metabolism, such as hydrogenases and oxidase. (2) The decreased activities of enzymes induced by Al or F increased significantly after mixing with each other, indicating that the simultaneous existence of Al and F decreased their ionic concentrations, so that strong antagonistic effects on the inhibition of enzymes were noticed since F or Al inhibited enzyme activities by their ions. (3) A high dose of Al intensively inhibited the enzymes related to the cell energy metabolism, which explained why less quantity of Al was absorbed in animals with high dose of Al than that of low dose Al seen in the absorption experiment. (4) The enzyme activities in the animals given high dose of Al mixed with F apparently were elevated in comparison with that in the animals given a pure high dose of Al which was the result of antagonistic effects of F on the inhibition of enzyme activities by Al, therefore the quantity of the absorbed Al was high.     

铝氟及莫混合液大鼠小肠原位灌流对酶活性的影响

本实验用 Al 32mmol/L,Al 170mmol/L,F 100mmol/L,Al32mmol/L+F100mmol/L,Al 170mmol/L+F100mmol/L五种溶液,分别原位灌流大鼠小肠40分钟,取空肠上段作组织学和15种酶的组织化学观察。结果显示:氟对脱氢酶(SDH,ICDH,NADHD,MDH,GDH,GPDH,LDH),氧化酶(CCO)和部分水解酶(ANAE,G 6 Pase,5′-N,ATPase,AlP,AcP)呈不同程度的抑制作用。铝则主要抑制脱氢酶及氧化酶。一定的铝氟混合后,酶活性较相应单纯铝及氟组均有升高。     

氟铝联合对体外人胚大脑神经细胞生长发育的影响

目的：探讨氟铝联合对体外人胚大脑神经细胞生长发育的影响。方法：采用体外人胚大脑神经细胞,分为６ 组观察氟铝共存对其生长发育的影响。结果：大脑神经细胞的存活数高剂量铝组低于低剂量铝组和对照组,有显著性差别（Ｐ＜ ０．０５）。结论：高剂量铝组可抑制人胚大脑神经细胞的生长发育,加入不同剂量氟并未对铝的作用产生明显的拮抗或协同作用     

Effects of Kupffer cells stimulated by triglyceride and very low-density lipoprotein on proliferation of rat hepatic stellate cells

ＯｂｊｅｃｔｉｖｅＴｏｓｔｕｄｙｔｈｅｅｆｅｃｔｓｏｆｔｒｉｇｌｙｃｅｒｉｄｅ,ｖｅｒｙｌｏｗｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ（ＶＬＤＬ）,ａｎｄＫｕｐｆｅｒｃｅｌｃｏｎｄｉｔｉｏｎｅｄｍｅｄｉｕｍ（ＫＣＣＭ）ｄｅｒｉｖｅｄｆｒｏｍｔｒｉｇｌ...     

大鼠摄入铝,氟及其不同比例混合物后血和骨中铝氟含量分析

Wistar rats were divided into 8 groups:control, 300 ppm F, 130 ppm F, 300 ppm Al, 1200 ppm Al, 130 ppm Al + 130 ppm F. 300 ppm Al + 300 ppm F and 1200 ppm Al + 300 ppm F. The chemicals were mixed into the standard diet. The animals were fed on the diets for 12 weeks. Contents of F, Al, Ca and P in the blood (or serum) and humerus were determined at the end of 12 weeks. The results showed that the level of F in the blood and bone in the unadulterated F group was increased, especially F in the bone reached a level more than 10 times that of the control. In the 3 mixture groups, blood F and bone F were lowered, while blood F was restored to normal level, but bone F was not nevertheless, the results showed that Al was in antagonism to the absorption of F. In the unadulterated Al groups, blood and bone Al did not parallel with the amount of Al administered. The level of Al in the median Al group was higher than that of the high Al group. Taking the level of blood and bone Al as a measure, when different doses of Al were administered with F, in the low and median dosage of Al, F was in antagonism to Al absorption, but in case of high dosage of Al, F was in potentiation to Al absorption. In all the experimental groups serum P was elevated, but serum Ca was not disturbed. Bone Ca and P were decreased only in the 3 groups with unadulterated F as well as unadulterated and adulterated high dosage of Al. Mechanism of the nonlinearity of Al absorption vs Al dosage, as well as the dual effect of F on the absorption of Al was proposed.     

氟铝联合对体外培养人胚成骨细胞生长发育的影响

目的：探讨氟铝联合对体外培养人胚成骨细胞生长发育的影响。方法：用无血清体外细胞培养方法，将成骨细胞分成空白对照组、低铝组、高铝组、低铝低氟组、高铝低氟组、高铝高氟组共6组，观察氟铝共存对其生长发育的影响。结果：加入氟铝后第14天和第16天，高铝组及高铝低氟组的细胞存活数均显著低于对照组、低铝组和低铝低氟组（P＜0．05）。高铝组的细胞变化坏死率明显高于对照组、低铝组及低铝低氟组（P＜0．05），高铝组加入不同剂量氟可抑制细胞的变性坏死率，但仍明显高于对照组、低铝组和低铝低氟组（P＜0．05），而低于高铝组（P＜0．05）。结论：高剂量铝可抑制体外培养的人胚成骨细胞的生长发育并对其产生一定的毒作用，在高铝情况下，加入一定量氟可抑制铝对成骨细胞的毒作用。     

铝对大鼠海马CA3区诱发电位的影响

实验在42只乌拉坦麻醉的SD大鼠上进行。用细胞外记录方法引导海马CA3区诱发放电,探讨向CA3区内微量注射不同剂量铝对诱发的群体锋电位(PS)的影响。结果:①不同剂量铝对PS波幅的影响不同,0.1和0.25mol/L AICI_3对PS无明显作用;0.5、1.0和2.0mol/L的AICI_3均可使PS波幅显著减小,且呈剂量-效应关系。②高渗盐水(2.0mol/L)和不同pH值(分别为6.4、3～4和7～8)的等渗盐水分别注入CA3区(均为1μl),诱发的PS幅度均无明显的改变。表明一定剂量的铝具有抑制CA3区PS波幅的作用,铝可能是抑制CA3区的突触传递过程。     

砖茶型氟铝联合中毒的实验研究

目的:复制砖茶型氟铝联合中毒动物模型。方法:通过检测动物骨、肝、肾、脑组织和血清中的氟、铝含量观察氟和铝在体内代谢的情况。结果:至实验期末各实验组动物体重均显著低于对照组(P<0.05),其中砖茶组动物体重降低更加显著(P<0.01);砖茶组动物各组织中氟、铝含量与对照组比较极显著增高(P<0.01)。结论:长期大量饮用砖茶水可导致砖茶型氟铝联合中毒;高氟可促进铝吸收并蓄积在骨组织中。     

环孢菌素A与细胞因子联合逆转白血病多药耐药性的体外研究

目的 探讨环孢菌素Ａ（ＣｓＡ）联合细胞因子对耐药细胞系Ｋ５６２／Ａ０２的逆转作用,方法 以甲基四唑蓝法测定柔红霉素（ＤＮＲ）的细胞毒性,用流式细胞仪技术测定细胞内罗丹明（Ｒｈ１２３）浓度,用ＲＴ－ＰＣＲ及ＪＳＢ－１抗体分别检测多药耐药（ＭＤＲ１）ｍＲＮＡ及其Ｐ糖蛋白的表达。结果 １μｍｏｌ／ＬＣｓＡ,５００Ｕ／ｍｌ干扰素２００Ｕ／ｍｌ白介素２（ＩＬ－２）均能增加ＤＮＲ对耐药细胞系Ｋ５６２／Ａ０２的     

高效液相色谱法测定尿中苯妥英代谢物浓度

A high-performance liquid chromatographic method for the determination of p-hydroxyphenytoin (p-HDPH) in human urine was reported. Following acid hydrolysis of urine sample at 90 degrees C for 1 h, an internal standard, nitrophenol, was added. The sample was extracted with n-hexane-ethyl acetate (50:50), and organic layer was evaporated. The residue was dissolved in methanol and chromatographed on an Ultrasphere-ODS column, using a mobile phase of phosphate buffer (0.03 mol/L, pH 6.0): methanol (65:35) at a flow rate of 0.8 ml/min. The eluent was monitored at 240 nm. The standard curve was linear within the range 5.0-200 micrograms/ml (r = 0.9998). Analytical recovery rates were 102.8 +/- 7.3% (p-HDPH 9.70 micrograms/ml, n = 5) and 104.9 +/- 6.4% (p-HDPH 54.70 micrograms/ml, n = 5). The cumulative recovery of p-HDPH in 0-12 h volunteers' urine samples accounted for 20% of the oral dose of 100 mg phenytoin sodium.