精浆5种生殖激素的研究

精浆对精子的生理功能有着重要影响。本文对51名正常生育男性和117名不育患者系统地观察了精浆 ICSH、FSH、PRL、T 和 E_2的变化,并进行相关分析。不育患者分五组与正常组比较,精子活力低下症 ICSH 升高(P<0.05),E_2降低(P<0.01)。无精子症 E_2亦下降(P<0.01)。精浆五种生殖激素及与精液质量的相关分析结果提示,精浆 ICSH 与 PRL 为正相关,并分别与精子密度、活力呈正相关。精子所处的微环境与生殖激素关系密切。     

血清生殖激素、精浆锌与睾丸生精障碍的关系

目的探讨无精子症患者血清生殖激素水平、精浆锌含量与睾丸生精状态的关系.方法对128例男性不育患者与30例正常对照者,进行了血清生殖激素(FSH,LH,T,PRL)检测、睾丸活检病理学检查、睾丸容积及精液分析,测定了40例无精子症患者和30例正常对照精浆的锌含量.结果无精子症组的精浆锌含量降低,与正常对照组比较存在显著性差异(P《0.05);睾丸源性无精子症血清FSH,LH升高,睾丸容积减少,睾丸容积小于15ml者,其T/LH值下降,与正常对照组间存在非常显著性差异(P《0.01),提示睾丸功能损伤,间质细胞功能受损. 结论血清FSH含量在鉴别睾丸源发性与梗阻性无精子症中是非常重要的指标,T/LH比值是判定睾丸间质细胞损伤的指标,睾丸生精障碍程度愈严重,其FSH,LH水平愈高,而L/LH值降低愈明显,精浆Zn及血清生殖激素的检测,在男性不育无精子症诊断和在判定睾丸功能的损伤程度中具有重要作用.     

血清生殖激素、精浆锌与睾丸生精障碍的关系

目的 探讨无精子症患者血清生殖激素水平、精浆锌含量与睾丸生精状态的关系。方法 对128例男性不育患者与30例正常对照者,进行了血清生殖激素（FSH,LH,T,PRL）检测、睾丸活检病理学检查、睾丸容积及精液分析,测定了40例无精子症患者和30例正常对照精浆的锌含量。结果 无精子症组的精浆锌含量降低,与正常对照组比较存在显著性差异（P<0.05）;睾丸源性无精子症血清FSH,LH升高,睾丸容积减少,睾丸容积小于15ml者,其T/LH值下降,与正常对照组间存在非常显著性差异（P<0.01）,提示睾丸功能损伤,间质细胞功能受损。结论 血清FSH含量在鉴别睾丸源发性与梗阻性无精子症中是非常重要的指标,T/LH比值是判定睾丸间质细胞损伤的指标,睾丸生精障碍程度愈严重,其FSH,LH水平愈高,而L/LH值降低愈明显,精浆Zn及血清生殖激素的检测,在男性不育无精子症诊断和在判定睾丸功能的损伤程度中具有重要作用。     

服棉酚者精浆中生殖激素的测定

血清中睾丸酮（T）、黄体生成素（LH）和促卵泡激素（FSH）的测定为临床不育症诊断中常用的检验方法，是了解间质细胞功能和生精功能的重要指标。我们对27例曾服棉酚的成年男子以及8例正常成年男子精浆和血浆中T、FSH、LH进行了测定，表明精浆T是判断间质细胞功能的一个更敏感指标。材料与方法一、实验对象1．正常组8例，婚后至少生育过一胎的健康男性。精液常规检查正常，精子计数＞40X106／ml，精子活率＞80％，畸型精子＜5％。2．20例口服棉酚1年9个月～9年2个月（总量为7．6～27．5g），停服22个月以上的成年男子。精液检查发现6…     

男性不育患者精子形态与生殖激素关系的初步研究

目的研究男性不育患者精子形态与生殖激素的关系,为男性不育的诊断和治疗提供理论依据。方法80例男性不育者按正常形态精子百分率分为四组:(1)对照组:正常形态精子百分率(正常形态精子百分率≥15%);(2)A组:严重畸形精子症(正常形态精子百分率<5%);(3)B组:中度畸形精子症(5%≤正常形态精子百分率<10%);(4)C组:轻度畸形精子症(10%≤正常形态精子百分率<15%)。测定血清生殖激素水平,并对结果进行比较分析。结果A组FSH、T水平显著高于对照组,而E2水平则刚好相反;正常形态精子百分比率与血清E2水平呈显著正相关关系(r=0.236,P=0.022),与FSH、T水平呈显著负相关关系(r=-0.201,P=0.038;r=-0.211,P=0.032)。结论血清生殖激素水平与男性不育患者精子形态存在一定的关系,检测血清生殖激素水平可为男性不育的诊治提供参考依据。     

72例不孕症妇女血清生殖激素异常结果分析

目的 :探讨不孕症妇女血清生殖激素异常的特点。方法 :回顾性分析 72例不孕症患者及 5 0例正常妇女的血清生殖激素水平。结果 :不孕症中生殖激素水平异常主要表现为 PCOS,占 5 9.72 % ;低 E2 水平 ,占 16.67% ;促性腺激素低水平 ,占 11.11% ;高泌乳素血症和卵巢早衰或卵巢发育不良。结论 :不孕症妇女生殖激素异常以促性腺激素水平异常最多见     

精索静脉曲张不育患者生殖激素测定

目的 探讨精索静脉曲张对外周血生殖激素水平的影响.方法 测定118例精索静脉曲张不育患者与30名健康体检者的外周血生殖激素FSH、LH和T水平.结果 精索静脉曲张(Ⅰ、Ⅱ、Ⅲ度)组FSH和LH与健康对照组之间的差异有统计学意义(P＜0.05),而在Ⅰ、Ⅱ、Ⅲ度组之间无明显差异(P＞0.05),Ⅰ、Ⅱ、Ⅲ度组T水平与健康对照组无明显差异(P＞0.05);A组FSH、LH、T水平与健康对照组的差异无统计学意义(P＞0.05),B、C组FSH、LH水平与对照组之间的差异有统计学意义(P＜0.05),T仅在重度少精子组较健康对照组降低.结论 精索静脉曲张可引起男性不育患者外周血生殖激素FSH、LH、T水平的不同程度的变化,FSH和LH与精子密度的减少有关,但精索静脉曲张对男性生殖激素的影响不随临床分度的增加而增强.     

生殖激素对卵泡凋亡的调节作用

本文目的旨在了解生殖激素对卵泡凋亡的调节作用，采用放免方法测定血清Fsh,LH E1和T含量，结果卵泡凋亡组Fsh,Lh,E2水平明显低于对照组，T则高于对照组，提示：卵泡的生长发育受Fsh,LH调控，而E2促进成熟卵泡的排出，T则促进卵泡凋亡。     

精索静脉曲张不育患者精浆生化结果及手术前后精液参数变化的研究

目的探讨精索静脉曲张不育患者精浆中酸性磷酸酶、锌水平的变化及手术前、后精液参数变化的情况。方法分别检测98例精索静脉曲张不育患者（精索静脉曲张不育组）和38例正常男性（对照组）的精浆中酸性磷酸酶、锌含量；观察42例行精索静脉高位结扎术的精索曲张不育患者（手术组）与对照组精液参数进行检测分析。结果精索静脉曲张不育组患者精浆中酸性磷酸酶含量均显著低于对照组（P〈0.01），精浆中锌含量明显低于对照组（P〈0．05）。手术组患者的精子密度、（A＋B）级活动力精子、活动率较对照组明显降低（P〈0．01），头部畸形率较对照组明显升高（P〈0.01），手术组中24例患者术后精索静脉曲张全部消失，精子质量较术前明显改善。结论精索静脉曲张引起精液质量下降，精浆中酸性磷酸酶、锌含量降低，从而造成男性不育，手术后精子质量有效改善。     

Trace elements in blood and seminal plasma and their relationship to sperm quality

The concentrations of cadmium, lead, selenium, and zinc in blood and seminal plasma were determined in 221 Singapore men. The relationship of trace element concentration to sperm density, motility, morphology, and semen volume was also investigated. The concentrations of elements were in the following descending order: Zn > Se > Pb > Cd. Except for zinc, the concentrations were generally higher in blood than in seminal plasma. The mean concentration of zinc in seminal plasma (ZnSP) was about 30 times higher than in blood (ZnB). A significant inverse correlation was observed between blood cadmium levels (CdB) and sperm density (r = −0.24, P < 0.05) in oligozoospermic men (sperm density below 20 million/mL) but not in normospermic men. Cadmium in seminal plasma (CdSP) was also associated with low semen volume (r = −0.29, P < 0.05). These findings suggest that cadmium may have an effect on the male reproductive system. In contrast, positive correlations were observed between concentrations of selenium and zinc in seminal plasma (SeSP and ZnSP) and sperm density in normospermic men but not in oligozoospermic men. The correlation coefficients with sperm density for SeSP and ZnSP were 0.35 (P < 0.05) and 0.41 (P < 0.01), respectively. The concentrations of lead in blood (PbB) or seminal plasma (PbSP) did not appear to have any correlation with the sperm parameters studied.     

A single LC-MS/MS validated method for tulathromycin quantification in plasma,seminal plasma,and urine to be applied in a pharmacokinetic study in bull

Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC-MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, seminal plasma, and urine. The approach is based on a quick protein precipitation with acetonitrile, filtration, and sample dilution before injection, allowing to rapidly process large batches of samples. Analytes separation was obtained using a BEH C18 (50 × 2.1 mm, 1.7 μm) column, maintained at 40°C with a chromatographic run of 5 min. The method was fully validated over concentration ranges suitable for field levels of tulathromycin found in each matrix (0.01–1 μg/ml for plasma, 0.05–5 μg/ml for seminal plasma, and 0.1–10 μg/ml for urine), showing good linearity during each day of testing (R² always >0.99). Accuracy and precision were within ±15% at all QC concentrations in all the three matrices. Furthermore, the use of tulathromycine-d7 as internal standard mitigated the potential impacts of matrix effect. The validated technique was successfully applied to samples collected during a pharmacokinetic study in bulls, allowing to monitor tulathromycin concentrations over time in the three matrices. To our knowledge, this is the first validated approach for LC-MS/MS quantification of tulathromycin in seminal plasma and urine.     

男性不育症患者精浆酸性磷酸酶对精浆生化指标的影响

目的研究男性不育症患者精浆酸性磷酸酶对精浆生化指标的影响。方法以539例男性不育症患者为研究对象,根据精浆酸性磷酸酶水平分为观察组和对照组,精浆酸性磷酸酶〉48．8U／mL为正常组,〈48．8U／mL为对照组,对两组患者精浆生化指标进行比较分析,通过相关性分析比较539例患者精浆酸性磷酸酶与精浆生化指标的相关性。结果观察组精浆锌明显低于对照组,精浆弹性蛋白酶明显高于对照组,曲组相比差异有统计学意义（P〈0．05）,精浆酸性磷酸酶与精浆锌的相关系数为0．612,呈明显正相关（P〈0．05）,与精浆弹性蛋白酶的相关系数为-0．325,呈明显负相关,而与精浆α-糖甘酶及果糖无相关性（P〉0．05）．结论联合检测精浆酸性磷酸酶、锌及弹性蛋白酶对于前列腺相关炎性疾病的诊断及治疗疗效的判定有重要的临床应用价值。     

男性不育患者精浆中表皮生长因子含量测定

为了探讨表皮生长因子与胃性精子发生的关系,采用放射免疫学方法对正常男性精浆中表皮生长因子（ＥＧＦ）含量进行测定。结果二组之间ＥＧＦ含一经ｔ′检验差异有显著性（Ｐ〈０．０５）,提示ＥＧＦ可能参与了男性生殖系统中精子发生的调控。     

Studies on the carboxyl terminal peptides of human seminal plasma inhibin (HSPI)

Observation of contradictory results with the in vitro assays for inhibin-like activity of the carboxyl terminal 28 amino acid peptide 67–94 with a disulfide loop, of human seminal plasma inhibin (HSPI), prompted us to synthesize both the linear and the cyclic peptides and test their ability to suppress the circulating levels of follicle stimulating hormone (FSH) in vivo in adult male rats. The linear peptide [Cys(Acm)^73,87] 67–94 of HSPI was synthesized by solid-phase peptide synthesis using fluorenylmethyloxycarbonyl (Fmoc) chemistry and a continuous-flow technology. The peptide was cyclized by direct iodine oxidation of the S-diacetamidomethyl peptide in dilute solution. In the in vivo assay the linear peptide did not affect the levels of FSH, whereas the cyclic peptide suppressed the levels of FSH significantly. Thus, the carboxyl terminal region of HSPI does have inhibin-like activity and perhaps has the active core of the protein.     

男性不育症患者精浆丙二醛和超氧化物岐化酶活性测定及临床价值研究

目的研究测定男性不育患者精浆MDA、SOD活性的临床价值。方法 120例患者采用随机数字表法分为三组,健康对照组、白细胞精子症组和少弱精子症组,比较MDA、SOD活性。结果少弱精子症组与白细胞精子症组精浆MDA明显高于健康对照组;白细胞精子症组精浆MDA明显高于少弱精子症组,差异有统计学意义(P<0.01)。少弱精子症组与白细胞精子症组精浆SOD明显低于健康对照组(P<0.01);白细胞精子症组精浆SOD明显低于少弱精子症组(P<0.01)。结论 ROS是导致男性不育症的一个重要病因,男性不育患者精浆MDA和SOD的测定对男性不育症的诊断具有重要的临床价值。     

慢性肾脏病患者血清甲状腺激素测定的临床意义

目的观察慢性肾脏病(CKD)患者血清甲状腺激素(TH)的变化。方法选择50例CKD患者作为观察组(CKD组),50例同期进行体检的健康人作对照组,测定血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺素(TSH)浓度及肾功能。结果 CKD组FT3(1.76±0.23)pmol/L、FT4(7.26±1.38)pmol/L,明显低于对照组的(5.42±0.56)pmol/L、FT4(16.54±3.73)pmol/L(P<0.05)。CKD组FT3、FT4分别与血尿素氮、肌酐呈负相关(P<0.01)。结论动态监测CKD患者血清TH的变化,对于判断患者的病情轻重与预后具有重要的参考价值。