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1.
Allele and haplotype frequencies of 11 Y-chromosomal short tandem repeats (STRs) included in the PowerPlex® Y Systems (Promega) were determined in a sample of 280 unrelated healthy autochthonous male individuals of the Chinese Xibe ethnic minority group living in Liaoning (northeast of China). The gene diversity values of the Y-STRs loci ranged from 0.3618 (DYS391) to 0.9686 (DYS385a/b). A total of 237 haplotypes were identified in the Y-STR loci, among which 207 were unique, 22 was found in 2 individuals, 4 were shared in 3 individuals, 3 were shared in 4 individuals, and 1 was shared in 5 individuals. The observed haplotype diversity value and discrimination capacity were 0.9984 and 0.8464, respectively. AMOVA tests demonstrated significant genetic heterogeneity between the Xibes and 16 reference populations from China and other Asian populations, justifying the establishment of local databases in the Chinese Xibe ethnic group for forensic application. 相似文献
2.
We studied and established a DNA database of 17 Y-STRs in a population sample of 133 unrelated individuals of Salar ethnic minority group, in order to investigate haplotype frequencies of Salar population, evaluate their usefulness in forensic applications, and enrich Chinese population genetic informational resources. Out of a total of 133 individuals 123 showed different haplotypes, while six haplotypes occurred more than once. The overall haplotype diversity for the Y-STRs loci was 0.9983, and the discrimination capacity was 0.9248. 相似文献
3.
Zhou Yi Wang Jun Song XingBo Lu XiaoJun Ding Liu Ying BinWu 《Legal medicine (Tokyo, Japan)》2010,12(2):108-111
We have co-amplified and analyzed 17 Y-chromosomal STRs loci (DYS19, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS437, DYS448, DYS456, DYS458, DYS635, YGATA-H4 and DYS385a/b) in 132 healthy unrelated autochthonous male individuals of Chinese Tibetan ethnic group residing in Lassa area of China. The gene diversity values for the Y-STRs loci ranged from a minimum 0.206 for DYS391 locus to a maximum of 0.912 for DYS385a/b locus in the populations. A total of 123 haplotypes were identified, among which 115 were unique and 8 occurred more than once. The overall haplotype diversity for 17 Y-STRs loci was 0.998. Research results will be valuable for forensic use in the regions and for Chinese population genetic study. 相似文献
4.
Zhu BF Wang ZY Yang CH Li XS Zhu J Yang G Huang P Liu Y 《International journal of legal medicine》2005,119(5):306-309
We have already coamplified minimal haplotypes (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385I/II), two additional loci, namely, DYS438, DYS439, and Amelogenin, in a single PCR using the Y-PLEX 12 kit. We investigated 107 unrelated male individuals from the Uigur ethnic group and studied the allelic frequency distribution and haplotype diversity of 11 Y-chromosomal STRs. A number of 43 alleles (nine STR loci) and 27 phenotypes (including DYS385) were detected, with frequencies ranging from 0.0092 to 0.6296. A total of 103 haplotypes were identified, among which 99 were individual-specific and four haplotypes were found twice. The haplotype diversity for these 12 Y-STR loci was 0.9993. 相似文献
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Guo H Yan J Jiao Z Tang H Zhang Q Zhao L Hu N Li H Liu Y 《Legal medicine (Tokyo, Japan)》2008,10(3):163-169
We have co-amplified 17 Y-chromosomal STRs (including DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a,b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y GATA H4) for samples of 143 unrelated male individuals of Chinese Hui Ethnic Minority Group. We obtained allelic frequency distribution and haplotype diversity of 17 Y-chromosomal STR for Hui population. A total of 136 different haplotypes were identified in 143 individuals, among which 129 were found only once, and seven haplotypes were found twice. The gene diversity values of STR loci ranged from 0.4161 (DYS391) to 0.9571 (DYS385a,b). The overall haplotype diversity for the 17 Y-STR loci was 0.9933, and the discrimination capacity was 0.9511. 相似文献
7.
Fang-Chin Wu Chin-Wen Ho Chang-En Pu Kuang-Yu Hu Sascha Willuweit Lutz Roewer David Hwang Liu 《International journal of legal medicine》2011,125(1):39-43
The distribution of Y-chromosomal short tandem repeat (Y-STR) haplotypes was determined in a population of Taiwanese Paiwan
aboriginals. Using 17 Y-STR markers, a total of 135 haplotypes were observed, 102 of which were unique. The overall haplotype
diversity for the 17 Y-STR loci tested was 0.9922 and the discrimination capacity was 0.6490. In addition, three novel intermediate
alleles at the DYS448 locus were also found. 相似文献
8.
In this study, allele frequencies for 29 autosomal short tandem repeats (STRs) and haplotype frequencies for 17 Y-chromosomal STRs of an Afghan population have been generated. Samples from 348 men and women originating from Afghanistan were analysed for the autosomal STRs, and the combined match probability was estimated to be 7.5?×?10?37. One hundred and sixty-nine men were analysed for the Y-chromosomal STRs, which resulted in 132 different haplotypes and a haplotype diversity of 0.995. 相似文献
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11.
《Legal medicine (Tokyo, Japan)》2014,16(3):173-175
Y-STR haplotype data were obtained in a population sample of 197 unrelated healthy male individuals of Chinese Tujia ethnic minority group residing in an autonomous county of Southern China using 17 Y-chromosome STR markers. A total of 197 haplotypes were identified in the set of Y-STR loci. The overall haplotype diversity for the Tujia population at 17 Y-STR loci was 1.0000 ± 0.0005. Genetic distance was estimated between this population and other 14 Chinese populations including Paiwan and Atayal populations of Taiwan, and Southern Han, Dong, Jing, Miao, Yao, Zhuang, Yi, Maonan, She, Hui, Sala, and Tibetan ethnic groups. The results demonstrated that the 17 Y-STR loci analyzed were highly polymorphic in Tujia ethnic group examined and hence useful for forensic cases, paternity testing, and population genetic studies. 相似文献
12.
《Forensic science international. Genetics》2009,3(4):e69-e70
Haplotypes and allele frequencies of 17 Y-chromosomal short tandem repeat (Y-STR) markers were examined using the AmpFlSTR Yfiler PCR Amplification Kit (Applied Biosystems) in a population sample of 1166 Japanese male volunteers in 6 prefectures: Miyagi, Yamagata, Osaka, Tottori, Fukuoka, and Okinawa. A total of 1058 haplotypes were observed from 1166 males, and the most common haplotype detected in 12 males had a frequency of 1.03% and the discrimination capacity was 0.907. The RST analysis showed statistically significant differences between Okinawa and the other subpopulations. 相似文献
13.
Florin Stanciu Veronica Cuţăr Sorina Pîrlea Veronica Stoian Ionel Marius Stoian Olivia Sevastre Oana Raluca Popescu 《Legal medicine (Tokyo, Japan)》2010,12(5):259-264
Haplotypes and allele frequencies for 17 STR loci included in AmpFlSTR YFiler kit (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATAH4, DYS437, DYS438 and DYS448) were determined in a sample of 122 unrelated males living in the South-East Romania. Genetic distances had been calculated and dendrograms had been generated for South-East Romanian population and other eighteen surrounding populations. 相似文献
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Zhu BF Shen CM Wang HD Yang G Yan JW Qin HX Guo JX Huang JF Jing H Liu XS 《International journal of legal medicine》2011,125(4):581-585
In the present study, we investigated 21 short tandem repeat (STR) loci (D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463,
D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435,
D5S2500), which are not included in the Combined DNA Index System and Amelogenin locus in 104 randomly selected healthy autochthonous
individuals from the Tibetan ethnic minority group residing in the Lhasa region, Tibet Autonomous Region of China. Allelic
frequencies, common forensic statistical parameters, and the Hardy–Weinberg equilibrium in this population were calculated
with a modified PowerState V12.xls. A total of 143 alleles were found in the Tibetan group with corresponding allelic frequencies
ranging from 0.005 to 0.582. The observed heterozygosity, the expected heterozygosity, the power of discrimination, the power
of exclusion, and the polymorphic information content ranged from 0.615 to 0.817, 0.559 to 0.787, 0.727 to 0.926, 0.310 to
0.632, and 0.488 to 0.760, respectively. Chi-square tests of the observed genotype frequencies and expected genotype frequencies
in the samples showed no departure from the Hardy–Weinberg equilibrium at all loci except for D5S2500. Our results demonstrate
that these 21 STRs are highly polymorphic and suitable for anthropological research, population genetics, and forensic paternity
testing and human individual identification in this region, and can enrich Chinese ethnical genetic informational resources. 相似文献
16.
《Forensic science international. Genetics》2010,4(1):e21-e22
Seventeen Y STR loci were typed in a population sample of 191 unrelated male individuals from Northern Greece. Haplotypes are presented for the following loci: DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438 and DYS448. The overall haplotype diversity was 0.9992. This database study provides significant additional information for the application of Y-chromosomal STRs to forensic identification efforts in Greece by nearly doubling both the number of individuals and the number of Y-loci typed from Greek populations. These samples have been previously typed for autosomal STRs [L. Kovatsi, T.J. Parsons, R.S. Just, J.A. Irwin, Genetic variation for 15 autosomal STR loci (PowerPlex 16) in a population sample from northern Greece, Forensic Sci. Int. 159 (2006) 61–63] and the mitochondrial DNA control region [J. Irwin, J. Saunier, K. Strouss, C. Paintner, T. Diegoli, K. Sturk, L. Kovatsi, A. Brandstatter, M.A. Cariolou, W. Parson, T.J. Parsons, Mitochondrial control region sequences from northern Greece and Greek Cypriots, Int. J. Legal Med. 122 (2008) 87–89]. 相似文献
17.
《Forensic science international. Genetics》2013,7(1):e7-e8
Allele and haplotype frequencies of 12 Y-chromosomal short tandem repeats (STRs) included in the PowerPlex® Y Systems (Promega) were determined in a sample of 150 unrelated healthy male individuals of Chinese Tuvans living in the Altay region of Xinjiang Uygur Autonomous Region. Allele frequencies and gene diversity for each Y-STR locus were determined. The observed haplotype diversity value was 0.9708. The present results can be used as Chinese Tuvan genetic information resources in routine population study and forensic analysis. 相似文献
18.
Evaluation of Y-chromosomal STRs: a multicenter study 总被引:23,自引:2,他引:21
Kayser M. Caglià A. Corach D. Fretwell N. Gehrig C. Graziosi G. Heidorn F. Herrmann S. Herzog B. Hidding M. Honda K. Jobling M. Krawczak M. Leim K. Meuser S. Meyer E. Oesterreich W. Pandya A. Parson W. Penacino G. Perez-Lezaun A. Piccinini A. Prinz M. Schmitt C. Schneider P. M. Szibor R. Teifel-Greding J. Weichhold G. de Knijff P. Roewer L. 《International journal of legal medicine》1997,110(3):125-133
A multicenter study has been carried out to characterize 13 polymorphic short tandem repeat (STR) systems located on the
male specific part of the human Y chromosome (DYS19, DYS288, DYS385, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, YCAI,
YCAII, YCAIII, DXYS156Y). Amplification parameters and electrophoresis protocols including multiplex approaches were compiled.
The typing of non-recombining Y loci with uniparental inheritance requires special attention to population substructuring
due to prevalent male lineages. To assess the extent of these subheterogeneities up to 3825 unrelated males were typed in
up to 48 population samples for the respective loci. A consistent repeat based nomenclature for most of the loci has been
introduced. Moreover we have estimated the average mutation rate for DYS19 in 626 confirmed father-son pairs as 3.2 × 10–3 (95% confidence interval limits of 0.00041–0.00677), a value which can also be expected for other Y-STR loci with similar
repeat structure. Recommendations are given for the forensic application of a basic set of 7 STRs (DYS19, DYS389I, DYS389II,
DYS390, DYS391, DYS392, DYS393) for standard Y-haplotyping in forensic and paternity casework. We recommend further the inclusion
of the highly polymorphic bilocal Y-STRs DYS385, YCAII, YCAIII for a nearly complete individualisation of almost any given
unrelated male individual. Together, these results suggest that Y-STR loci are useful markers to identify males and male lineages
in forensic practice.
Received: 30 December 1996 / Received in revised form: 26 February 1997 相似文献
19.
Highly informative Y-chromosomal haplotypes by the addition of three new STRs DYS437, DYS438 and DYS439 总被引:1,自引:0,他引:1
Grignani P Peloso G Fattorini P Previderè C 《International journal of legal medicine》2000,114(1-2):125-129
The Y chromosome STRs DYS437, DYS438 and DYS439 were selected from publicly available genome databases and used to analyse
an Italian population sample. A tetraplex PCR reaction including the highly informative DYS385 locus, was set up and used
for the analysis of 131 male samples to determine allele frequencies and STR diversity values. The number of different haplotypes
and the haplotype diversity value found from the analysis of the STRs included in the tetraplex reaction were very similar
to those found from the analysis of the basic set of 7 Y-STRs (DYS19, DYS389I/II, DYS390, DYS391, DYS392 and DYS393) previously
carried out on the same population sample. By combining the allelic states of the 11 Y-chromosomal STRs we could construct
highly informative haplotypes that allowed the discrimination of 93.8% (120 out of 128) of the samples tested. This approach
represents a very powerful tool for individual identification and paternity testing in forensic medicine.
Received: 29 November 1999 / Accepted: 17 March 2000 相似文献
20.
Allele frequency data and statistical parameters for D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA loci were determined in a sample of 120 healthy unrelated individuals of Chinese Yi ethnic minority group living in Yunnan province, China. We observed 132 alleles with allelic frequencies ranging from 0.0042 to 0.5333. The forensic statistical parameters from the data of all the loci showed high values. All loci were in accordance with Hardy-Weinberg equilibrium (p>0.05). The obtained frequency distributions were compared with previously published other population data, and significant differences were found between Yi population and Korean, Chinese Tibetan, Uigur, Ewenki, Han, Hui population at some STR loci. Our results of present study were valuable for forensic application and Chinese population genetic studies. These population data enriched Chinese genetic informational resources. 相似文献