共查询到20条相似文献,搜索用时 15 毫秒
1.
R. Lucarini M.G. Tozatti M.L.A. Silva V.M.M. Gimenez P.M. Pauletti M. Groppo I.C.C. Turatti W.R. Cunha C.H.G. Martins 《Brazilian journal of medical and biological research》2015,48(9):822-830
This paper reports on the in vitro antibacterial and in
vivo anti-inflammatory properties of a hydroethanolic extract of the
aerial parts of Gochnatia pulchra (HEGP). It also describes the
antibacterial activity of HEGP fractions and of the isolated compounds genkwanin,
scutellarin, apigenin, and 3,5-O-dicaffeoylquinic acid, as evaluated by a broth
microdilution method. While HEGP and its fractions did not provide promising results,
the isolated compounds exhibited pronounced antibacterial activity. The most
sensitive microorganism was Streptococcus pyogenes, with minimum
inhibitory concentration (MIC) values of 100, 50 and 25 µg/mL for genkwanin and the
flavonoids apigenin and scutellarin, respectively. Genkwanin produced an MIC value of
25 µg/mL against Enterococcus faecalis. A paw edema model in rats
and a pleurisy inflammation model in mice aided investigation of the
anti-inflammatory effects of HEGP. This study also evaluated the ability of HEGP to
modulate carrageenan-induced interleukin-1 beta (IL-1β), tumor necrosis factor alpha
(TNF-α), and monocyte chemoattractant protein-1 (MCP-1) production. Orally
administered HEGP (250 and 500 mg/kg) inhibited carrageenan-induced paw edema.
Regarding carrageenan-induced pleurisy, HEGP at 50, 100, and 250 mg/kg diminished
leukocyte migration by 71.43%, 69.24%, and 73.34% (P<0.05), respectively. HEGP
suppressed IL-1β and MCP-1 production by 55% and 50% at 50 mg/kg (P<0.05) and 60%
and 25% at 100 mg/kg (P<0.05), respectively. HEGP abated TNF-α
production by macrophages by 6.6%, 33.3%, and 53.3% at 100, 250, and 500 mg/kg
(P<0.05), respectively. HEGP probably exerts anti-inflammatory effects by
inhibiting production of the pro-inflammatory cytokines TNF-α, IL-1β, and MCP-1. 相似文献
2.
Hajhashemi V Sadeghi H Minaiyan M Movahedian A Talebi A 《Clinics (S?o Paulo, Brazil)》2010,65(11):1183-1187
OBJECTIVE:
The present study was designed to further investigate the effect of amitriptyline, a classical tricyclic antidepressant, on carrageenan-induced paw edema in rats.METHODS:
First, amitriptyline was administered intraperitoneally (i.p.) at doses of 20, 40 and 80 mg kg-1, 30 min before subplantar injection of carrageenan. Second, amitriptyline was given intracerebroventriculary or intrathecally at doses of 25, 50 and 100 µg/rat, 30 min prior to carrageenan challenge. Third, the effect of adrenergic receptor antagonists such as propranolol (10 mg kg-1, i.p.), prazosin (4 mg kg-1, i.p.) and yohimbine (10 mg kg-1, i.p.) and an opioid receptor antagonist (naloxone, 4 mg kg-1, i.p.) on the anti-inflammatory effect of amitriptyline (40 mg kg-1, i.p.) was investigated.RESULTS:
Our data confirm that intraperitoneally administered amitriptyline exhibits a marked anti-inflammatory effect on carrageenan-induced paw edema in rats 4 h postcarrageenan challenge (P < 0.001). Intracerebroventricular (i.c.v.) administration of amitriptyline also reduced the development of paw edema at 4 h postcarrageenan (P < 0.001), but intrathecal (i.t.) application of amitriptyline failed to alter the degree of paw swelling. Furthermore, the applied antagonists did not modify the anti-inflammatory effect of amitriptyline.CONCLUSION:
These results support the view that amitriptyline has a considerable anti-inflammatory effect on carrageenan-induced paw edema in rats and suggest that at least a part of this property could be mediated through supraspinal sites. Moreover, it seems unlikely that the investigated adrenergic and opioid receptors have a significant role in this effect of amitriptyline. 相似文献3.
Seewaboon Sireeratawong Kanjana Jaijoy Noppamas Soonthornchareonnon 《African journal of traditional, complementary, and alternative medicines》2013,10(2):246-250
The anti-inflammatory and antinociceptive activities of Triphala recipe were studied in animal models. Triphala recipe (4 mg/ear) significantly exhibited an inhibitory effect on the ear edema formation induced by ethyl phenylpropiolate-induced, but not on the arachidonic acid-induced ear edema in rats. Furthermore, Triphala recipe at the doses of 300, 600 and 1,200 mg/kg significantly reduced carrageenan-induced hind paw edema. Next, the anti-inflammatory action in chronic inflammation was measured using the cotton pellet-induced granuloma formation assay in rats. Triphala recipe (1,200 mg/kg) reduced neither transudative weight nor granuloma formation. It also did not affect on body weight gain and thymus weight indicating that Triphala recipe does not have a steroid-like effect. In antinociceptive study, Triphala recipe (300, 600, 1,200 mg/kg), elicited significant inhibitory effect on both phases, especially in late phase, of the formalin test in mice suggesting that the antinociceptive action of Triphala recipe may be via both peripheral and at least partly centrally acting. 相似文献
4.
Bukola Olunike Oyebanji Adebowale Bernard Saba Olayinka Ayotunde Oridupa 《African journal of traditional, complementary, and alternative medicines》2014,11(1):30-33
Background
The anti-inflammatory and anti-nociceptive activity of betulinic acid (BA) was investigated in this study. The triterpene was isolated from the ethyl acetate extract of Tetracera potatoria and its structure was verified by IR and NMR spectroscopy. The bioactivity of this compound was assessed using carrageenan-induced paw oedema in rats and carrageenan-induced pulmonary oedema in mice for the anti-inflammatory activity, while acetic acid-induced writhing test in mice and zymosan-induced fever in rats were used for analgesic test.Materials and Methods
Rats and mice were randomly divided into groups of five animals. For each experiment, betulinic acid at 10, 20 or 40mg/kg b.w was administered intraperitoneally to the first three groups respectively. The fourth group was administered with indomethacin (10mg/kg) or acetylsalicylic acid (150mg/kg), while the fifth group was administered with distilled water (10ml/kg). Data obtained were expressed as mean±S.E.M and significant differences were determined at p<0.05.Results
BA significantly reduced carrageenan-induced paw oedema by 11.0%, 45.7%, 68.6% or pulmonary oedema by 25.6, 29.2 and 45.13% dose dependently. 40 mg/kg of BA inhibited paw oedema by 68.6% comparably to acetylsalicylic acid (71.4%) or indomethacin (51.33%) respectively. Abdominal writhing was also significantly (p<0.05) reduced to 17.20 writhes by BA (40mg/kg) comparable to Indomethacin (16.3writhes). Fever was inhibited by BA most significantly by 3hours post-injection of zymosan (1.00, 1.45, 0.00°C) and this inhibitory effect was higher than that observed for acetylsalicylic acid (0.30°C).Conclusion
Betulinic acid derived from Tetracera potatoria exhibited potent anti-inflammatory, analgesic or antipyrexic activity which is comparable to indomethacin or acetylsalicyclic acid. 相似文献5.
Pratibha N Saxena VS Amit A D'Souza P Bagchi M Bagchi D 《International journal of tissue reactions》2004,26(1-2):43-51
Allergic rhinitis is an immunological disorder and an inflammatory response of nasal mucosal membranes. Allergic rhinitis, a state of hypersensitivity, occurs when the body overreacts to a substance such as pollens or dust. A novel, safe polyherbal formulation (Aller-7/NR-A2) has been developed for the treatment of allergic rhinitis using a unique combination of extracts from seven medicinal plants including Phyllanthus emblica, Terminalia chebula, Terminalia bellerica, Albizia lebbeck, Piper nigrum, Zingiber officinale and Piper longum. Since inflammation is an integral mechanistic component of allergy, the present study aimed to determine the anti-inflammatory activity of Aller-7 in various in vivo models. The efficacy of Aller-7 was investigated in compound 48/80-induced paw edema both in Balb/c mice and Swiss Albino mice, carrageenan-induced paw edema in Wistar Albino rats and Freund's adjuvant-induced arthritis in Wistar Albino rats. The trypsin inhibitory activity of Aller-7 was also determined and compared with ovomucoid. At a dose of 250 mg/kg, Aller-7 demonstrated 62.55% inhibition against compound 48/80-induced paw edema in Balb/c mice, while under the same conditions prednisolone at an oral dose of 14 mg/kg exhibited 44.7% inhibition. Aller-7 significantly inhibited compound 48/80-induced paw edema at all three doses of 175, 225 or 275 mg/kg in Swiss Albino mice, while the most potent effect was observed at 225 mg/kg. Aller-7 (120 mg/kg, p.o.) demonstrated 31.3% inhibition against carrageenan-induced acute inflammation in Wistar Albino rats, while ibuprofen (50 mg/kg, p.o.) exerted 68.1% inhibition. Aller-7 also exhibited a dose-dependent (150-350 mg/kg) anti-inflammatory effect against Freund's adjuvant-induced arthritis in Wistar Albino rats and an approximately 63% inhibitory effect was observed at a dose of 350 mg/kg. The trypsin inhibitory activity of Aller-7 was determined, using ovomucoid as a positive control. Ovomucoid and Aller-7 demonstrated IC50 concentrations at 1.5 and 9.0 microg/ml, respectively. These results demonstrate that this novel polyherbal formulation is a potent anti-inflammatory agent that can ameliorate the symptoms of allergic rhinitis. 相似文献
6.
A.L.R. Barbosa C.A. Pinheiro G.J. Oliveira J.N.L. Torres M.O. Moraes R.A. Ribeiro M.L. Vale M.H.L.P. Souza 《Brazilian journal of medical and biological research》2012,45(6):531-536
Implantation of Walker 256 tumor decreases acute systemic inflammation in rats. Inflammatory hyperalgesia is one of the most important events of acute inflammation. The L-arginine/NO/cGMP/K+ATP pathway has been proposed as the mechanism of peripheral antinociception mediated by several drugs and physical exercise. The objective of this study was to investigate a possible involvement of the NO/cGMP/K+ATP pathway in antinociception induced in Walker 256 tumor-bearing male Wistar rats (180-220 g). The groups consisted of 5-6 animals. Mechanical inflammatory hypernociception was evaluated using an electronic version of the von Frey test. Walker tumor (4th and 7th day post-implantation) reduced prostaglandin E2- (PGE2, 400 ng/paw; 50 µL; intraplantar injection) and carrageenan-induced hypernociception (500 µg/paw; 100 µL; intraplantar injection). Walker tumor-induced analgesia was reversed (99.3% for carrageenan and 77.2% for PGE2) by a selective inhibitor of nitric oxide synthase (L-NAME; 90 mg/kg, ip) and L-arginine (200 mg/kg, ip), which prevented (80% for carrageenan and 65% for PGE2) the effect of L-NAME. Treatment with the soluble guanylyl cyclase inhibitor ODQ (100% for carrageenan and 95% for PGE2; 8 µg/paw) and the ATP-sensitive K+ channel (KATP) blocker glibenclamide (87.5% for carrageenan and 100% for PGE2; 160 µg/paw) reversed the antinociceptive effect of tumor bearing in a statistically significant manner (P < 0.05). The present study confirmed an intrinsic peripheral antinociceptive effect of Walker tumor bearing in rats. This antinociceptive effect seemed to be mediated by activation of the NO/cGMP pathway followed by the opening of KATP channels. 相似文献
7.
《Immunopharmacology and immunotoxicology》2013,35(3):336-340
AbstractObjective: Belamcanda chinensis has been used in oriental medicine for the treatment of inflammatory diseases. Tectorigenin is a main compound in B. chinensis and possess inhibitory activity against inflammatory responses. Thus, the current study aimed at evaluating toxicity as well as analgesic and anti-inflammatory effects of tectorigenin in animal models.Methods: Tectorigenin was employed to evaluate acute and subacute toxicity. Acetic acid-induced writhing in mice was used for analgesic test. The anti-inflammatory activity was tested in carrageenan-induced paw edema.Results: LD50 of tectorigenin was 1.78?g/kg p.o. in mice and no toxic symptoms were observed at doses up to 300?mg/kg in a subacute toxicity test during 28-day treatment. Tectorigenin at doses of 50 and 100?mg/kg had an analgesic effect on acetic acid-induced acute visceral pain in mice. In inflammatory rat model, tectorigenin at 60?mg/kg significantly reduced carrageenan-induced edema.Conclusion: We demonstrated that tectorigenin is a safe and promising analgesic and anti-inflammatory agent. 相似文献
8.
Sachin S. Sakat Kamaraj Mani Yulia O. Demidchenko Evgeniy A. Gorbunov Sergey A. Tarasov Archna Mathur Oleg I. Epstein 《Inflammation》2014,37(1):1-9
The study was aimed to investigate the effect of technologically treated diclofenac (release-active dilutions of diclofenac (RAD of diclofenac)) on anti-inflammatory activity of diclofenac in carrageenan-induced rat paw edema model. Ninety male Wistar albino rats (6–8 weeks) divided into nine groups (n?=?10) were used. Anti-inflammatory activity was assessed at 1, 2, 3, 4, and 6 h after subplantar injection of carrageenan (0.1 ml of a 1 % solution in normal saline). Diclofenac alone was studied at 5 and 20 mg/kg, RAD of diclofenac alone at 7.5 ml/kg and their combination at 5 and 7.5 ml/kg, respectively. Diclofenac reduced (p?<?0.05 at least) paw edema at all time points. RAD of diclofenac enhanced (p?<?0.05) anti-inflammatory effect of diclofenac (5 mg/kg) at 2, 4, and 6 h on concurrent and at 2 and 4 h on sequential administration. Moreover at 2 h, anti-inflammatory effect of combination treatment reached values comparable to those of diclofenac (20 mg/kg). In conclusion, RAD of diclofenac enhanced anti-inflammatory effect of diclofenac. 相似文献
9.
Shekoufeh Nikfar Roja Rahimi Ali Rezaie Mohammad Abdollahi 《Archives of Medical Science》2010,6(2):236-244
Introduction
Natalizumab is a new humanized monoclonal antibody used in multiple sclerosis (MS). The aim of this meta-analysis was to evaluate the efficacy and tolerability of this drug in relapsing MS. PubMed, Scopus, Web of Science, and Cochrane Central Register of Controlled Trials were searched for studies that investigated the efficacy and/or tolerability of natalizumab in MS. Data were collected from 1966 to 2008 (up to October).Material and methods
The search terms were: “multiple sclerosis” or “MS” and “natalizumab”. “Mean change in Expanded Disability Status Scale (EDSS)”, “number of patients with at least one relapse”, and “number of patients with at least one new gadolinium (Gd)-enhancing lesion” were the key outcomes of interest for assessment of efficacy. “Any adverse events”, “serious adverse events”, “death”, and “withdrawal because of adverse events” were the key outcomes for tolerability. Among existing trials, four randomized placebo controlled clinical trials met our criteria and were included.Results
Pooled relative risk for at least one relapse in four trials including all doses was 0.7, with a non-significant RR (95% CI: 0.42–1.17, p = 0. 17). Summary RR for at least one relapse in two trials in which doses of 3 mg/kg or 6 mg/kg or 300 mg every 4 weeks were administered gave a value of 0.5 asa significant RR (95% CI: 0.42–0.61, p < 0.0001). The summary RR for at least one new Gd-enhancing lesion was 0.22, a non-significant RR (95% CI: 0.05–1.01, p = 0.051). Three deaths were reported in the natalizumab group. Comparing adverse events between natalizumab and placebo yielded a non-significant RR of 0.99 (95% CI: 0.96–1.01, p = 0.34) for any adverse events (n = 3), and a significant RR of 0.39 (95% CI: 0.29–0.52, p < 0.0001) for serious adverse events (n = 2). The summary RR for withdrawal due to adverse events by natalizumab vs. placebo therapy between two trials was 1.43, a non-significant RR (95% CI: 0.68–3.02, p = 0.35).Conclusions
It seems that using 3 or 6 mg/kg every 4 weeks is the best method of administration of natalizumab for preventing relapse and occurrence of new Gd-enhancing lesions. The current data on the efficacy and safety of natalizumab are insufficient to reach a convincing conclusion and thus further clinical trials are still needed. 相似文献10.
Antonia Amanda Cardoso de Almeida Renan Oliveira Silva Lucas Antonio Duarte Nicolau Tarcísio Vieira de Brito Damião Pergentino de Sousa André Luiz dos Reis Barbosa Rivelilson Mendes de Freitas Luciano da Silva Lopes Jand-Venes Rolim Medeiros Paulo Michel Pinheiro Ferreira 《Inflammation》2017,40(2):511-522
D-limonene epoxidation generates (+)-limonene epoxide, an understudied compound in the pharmacologically point of view. Herein, we investigated the anti-inflammatory and antinociceptive potentialities of (+)-limonene epoxide and suggested a mechanism of action. The anti-inflammatory potential was analyzed using agents to induce paw edema, permeability, and myeloperoxidase (MPO) activity. Pro-inflammatory cytokines and cell migration of peritoneal cells were also assessed. Antinociceptive effects were evaluated by writhing test induced by acetic acid, formalin, and hot plate assays and contribution of opioid pathways. Pretreated animals with (+)-limonene epoxide showed reduced carrageenan-induced paw edema in all doses (25, 50, and 75 mg/kg) (P?<?0.05). At 75 mg/kg, it suppressed edema provoked by compound 48/80, histamine, prostaglandin E2, and serotonin and reduced permeability determined by Evans blue and MPO activity. It also reduced leukocytes, neutrophils, and IL-1β levels in the peritoneal cavity in comparison with carrageenan group (P?<?0.05). (+)-Limonene epoxide diminished abdominal contortions induced by acetic acid (78.9%) and paw licking times in both 1 (41.8%) and 2 (51.5%) phases and a pretreatment with naloxone (3 mg/kg) reverted the antinociceptive action in morphine- and (+)-limonene epoxide-treated groups (P?<?0.05). Additionally, it enlarged response times to the thermal stimulus after 60 and 90 min. In conclusion, (+)-limonene epoxide inhibited release/activity of inflammatory mediators, vascular permeability, migration of neutrophils and displayed systemic and peripheral analgesic-dependent effects of the opioid system. 相似文献
11.
Objective: To elucidate the site of action of perfluorooctanoic acid (PFOA) in the carrageenan model of peripheral inflammation.Subjects: Male Sprague-Dawley rats.Treatment: We first compared the anti-edema effects of systemic PFOA (50–150 mg/kg) with prototypical nonsteroidal (acetylsalicylic acid, ASA, 50–200 mg/kg) and steroidal (dexamethasone, 0.5–5.0 mg/kg) drugs after the intraplantar injection of carrageenan (1%). We then compared the anti-edema effects of systemic PFOA with local intraplantar (10 mg/kg), and intracerebroventricular (i.c.v., 0.1–50 μg) routes of administration.Results: Systemic PFOA was at least as or more efficacious than ASA or dexamethasone in reducing carrageenan-induced edema. RU-486 did not change the anti-edema effect of PFOA, ruling out a contribution of endogenous release of glucorticoids. I. c. v. PFOA, but not perfluorooctanes, dramatically reduced multiple signs of inflammation at doses well below the systemically-effective dose. We conclude that the anti-edema effect of high systemic doses of PFOA (≥100 mg/kg, i. p.) is mediated in part by actions in the brain.Received 30 August 2004; returned for revision 29 October 2004; accepted by G. Geisslinger 8 February 2005 相似文献
12.
L.A.D. Nicolau R.O. Silva S.R.B. Damasceno N.S. Carvalho N.R.D. Costa K.S. Arag?o A.L.R. Barbosa P.M.G. Soares M.H.L.P. Souza J.V.R. Medeiros 《Brazilian journal of medical and biological research》2013,46(8):708-714
Our objective was to investigate the protective effect of Lawesson''s reagent, an
H2S donor, against alendronate (ALD)-induced gastric damage in rats.
Rats were pretreated with saline or Lawesson''s reagent (3, 9, or 27 µmol/kg,
po) once daily for 4 days. After 30 min, gastric damage was
induced by ALD (30 mg/kg) administration by gavage. On the last day of treatment, the
animals were killed 4 h after ALD administration. Gastric lesions were measured using
a computer planimetry program, and gastric corpus pieces were assayed for
malondialdehyde (MDA), glutathione (GSH), proinflammatory cytokines [tumor necrosis
factor (TNF)-α and interleukin (IL)-1β], and myeloperoxidase (MPO). Other groups were
pretreated with glibenclamide (5 mg/kg, ip) or with glibenclamide (5
mg/kg, ip)+diazoxide (3 mg/kg, ip). After 1 h, 27
µmol/kg Lawesson''s reagent was administered. After 30 min, 30 mg/kg ALD was
administered. ALD caused gastric damage (63.35±9.8 mm2); increased levels
of TNF-α, IL-1β, and MDA (2311±302.3 pg/mL, 901.9±106.2 pg/mL, 121.1±4.3 nmol/g,
respectively); increased MPO activity (26.1±3.8 U/mg); and reduced GSH levels
(180.3±21.9 µg/g). ALD also increased cystathionine-γ-lyase immunoreactivity in the
gastric mucosa. Pretreatment with Lawesson''s reagent (27 µmol/kg) attenuated
ALD-mediated gastric damage (15.77±5.3 mm2); reduced TNF-α, IL-1β, and MDA
formation (1502±150.2 pg/mL, 632.3±43.4 pg/mL, 78.4±7.6 nmol/g, respectively);
lowered MPO activity (11.7±2.8 U/mg); and increased the level of GSH in the gastric
tissue (397.9±40.2 µg/g). Glibenclamide alone reversed the gastric protective effect
of Lawesson''s reagent. However, glibenclamide plus diazoxide did not alter the
effects of Lawesson''s reagent. Our results suggest that Lawesson''s reagent plays a
protective role against ALD-induced gastric damage through mechanisms that depend at
least in part on activation of ATP-sensitive potassium (KATP)
channels. 相似文献
13.
Edfranck de Sousa Oliveira Vanderlei Ianna Wivianne Fernandes de Ara��jo Ana Lu��za Gomes Quinder�� Bruno Pedrosa Fontes Ygor Raphael Gomes Eloy Jos�� Ari��vilo Gurgel Rodrigues Antonio Alfredo Rodrigues e Silva Hell��ada Vasconcelos Chaves Roberta Jeane Bezerra Jorge Dalgimar Beserra de Menezes Jana��na Serra Azul Monteiro Evangelista Mirna Marques Bezerra Norma Maria Barros Benevides 《Inflammation research》2011,60(12):1121-1130
Objectives
The aim of this study was to investigate the involvement of the hemoxigenase-1 (HO-1) pathway in the anti-inflammatory action of a sulfated polysaccharide from the red seaweed Gracilaria birdiae (SP-Gb).Methods
SP-Gb (5, 10 and 20?mg/kg) was administered to Wistar rats in a peritonitis model using carrageenan or a paw edema model using carrageenan or dextran. To analyze the involvement of HO-1 in the anti-inflammatory activity of SP-Gb, the animals were pretreated subcutaneously with a specific HO-1 inhibitor (ZnPP IX). To evaluate the systemic effects, SP-Gb (10?mg/kg) was administered to mice intraperitoneally before waiting for 48?h or for 14?days.Results
SP-Gb (10?mg/kg) caused an anti-inflammatory effect that was evidenced by a decrease in leukocytes in the peritoneal cavity. SP-Gb also reduced the paw edema induced by carrageenan and inhibited the paw edema induced by dextran in the first half-hour. After being inhibited by ZnPP IX, the anti-inflammatory effect of SP-Gb on carrageenan-induced rat paw edema was not observed. SP-Gb did not cause mortality or significant changes in the biochemical, hematological and histopathological parameters.Conclusion
SP-Gb may be used as a tool for further investigations into the inflammatory processes associated with the hemoxigenase-1 pathway. 相似文献14.
Anti-inflammatory and antipyretic effects of boldine 总被引:1,自引:0,他引:1
N. Backhouse C. Delporte M. Givernau B. K. Cassels A. Valenzuela H. Speisky 《Inflammation research》1994,42(3-4):114-117
Boldine, and antioxidant alkaloid isolated fromPeumus boldus, exhibits a dose-dependent anti-inflammatory activity in the carrageenan-induced guinea pig paw edema test with an oral ED50 of 34 mg/kg. Boldine also reduces bacterial pyrogen-induced hyperthermia in rabbits to an extent which varied between 51% and 98% at a dose of 60 mg/kg p.o.In vitro studies carried out in rat aortal rings revealed that boldine is an effective inhibitor of prostaglandin biosynthesis, promoting 53% inhibition at 75 M. The latterin vitro effect may be mechanistically linked to the anti-inflammatory and antipyretic effects of boldine exertedin vivo. 相似文献
15.
Thiago Henrique Costa Marques Maria Leonildes Boavista Gomes Castelo Branco Marques Jand-Venes R. Medeiros Renan Oliveira Silva André Luiz dos Reis Barbosa Tamires Cardoso Lima Damião Pergentino de Sousa Rivelilson Mendes de Freitas 《Inflammation》2014,37(3):966-977
Cyane-carvone (CC) was studied to elucidate its anti-inflammatory, antinociceptive, and antioxidant effects in Mus musculus. Anti-inflammatory (bradykinin, histamine, prostaglandin E2, serotonin, and carrageenan) and antinociceptive (acetic acid and formalin) models were utilized. Myeloperoxidase activity, interleukin (IL)-1β, tumor necrosis factor alpha (TNF-α), and glutathione (GSH) levels were evaluated. Analysis of variance followed by Student-Newman-Keuls’ test was done. Results were compared with control groups (significantly when p?<?0.05). In bradykinin, histamine, prostaglandin E2, and serotonin tests, 75 mg/kg CC decreased significantly paw edema (t?=?30, 60, 90, and/or 120 min). In carrageenan test, 50 and 75 mg/kg CC (t?=?3 h and t?=?4 h) and 25 mg/kg CC (t?=?4 h) decreased significantly paw edema. CC (75 mg/kg) inhibited significantly mieloperoxidase activity and decreased IL-1β and TNF-α, and all doses increased GSH levels. CC (75 mg/kg) decreased significantly the number of contortions of animals and time of licking (phase 2). CC showed anti-inflammatory, antinociceptive, and antioxidant effects in mice. 相似文献
16.
Al-Majed A. A. Khattab M. Raza M. Al-Shabanah O. A. Mostafa A. M. 《Inflammation research》2003,52(9):378-382
Objective: To investigate whether aminoguanidine (AG) treatment enhances the anti-inflammatory effect of diclofenac in an acute inflammation model in rats.Material and methods: In 48 rats carrageenan-induced paw edema was used as an acute inflammation model. Inflammatory activity was assessed at 1.5, 3 and 6 h after sub-planter injection of carrageenan (0.1 ml of a 1% solution in 0.85% saline). The anti-inflammatory effect of diclofenac (25 mg/kg, i.p.) was studied in comparison to that of the selective inducible nitric oxide synthase (iNOS) inhibitor, AG, and of nitric oxide donor, sodium nitroprusside (SNP).Results: AG, failed to inhibit inflammation during the first 3 h following carrageenan administration, but caused a slight, although statistically insignificant inhibition at 6 h. Diclofenac significantly reduced the carrageenan-induced edema in rat paw at all the time points studied. Administration of diclofenac after AG pretreatment caused significant (P < 0.001) reduction in edema that was double that of diclofenac alone 6 h after carrageenan injection. Administration of SNP as a single dose after AG pretreatment prevented any potentiation of anti-inflammatory response that was observed in the case of AG combined with diclofenac treatment.Conclusion: These results show that AG markedly potentiates the anti-inflammatory activity of diclofenac at 6 h and this potentiation effect is nitric oxide-dependent.Received 28 October 2002; returned for revision 1 April 2003; accepted by I. Ahnfelt-Rønne 5 May 2003 相似文献
17.
Bovine CuZn superoxide dismutase (SOD: 1, 3 and 10 mg/kg) dose-dependently reduced carrageenan-induced paw edema in rats when administered intravenously 30 min before irritant injection. However, heat-treated SOD (10 mg/kg) was as effective as native SOD (10 mg/kg) although the enzymic activity was reduced to 9.7%. Examination of the contaminants of the native SOD revealed a fairly large amount of endotoxin-like activity, 47 ng asEscherichia coli endotoxin per mg, and 59.6% of this activity remained after heat treatment. Bovine CuZn SOD (1, 3 and 10 mg/kg), which contained negligible endotoxin-like materials and 1.5 times more enzyme units, had no effect on edema under the same conditions. Furthermore,Escherichia coli endotoxin (10, 100 and 1000 ng/kg) reduced edema dose-dependently. These results suggest that contamination by endotoxin-like materials is responsible for the anti-inflammatory action of the SOD preparation we observed. Hence, the anti-inflammatory action of contaminating endotoxin-like materials may lead to misinterpretation as a protective effect of SOD unless stringent precautions are taken against endotoxin-like contaminants in the SOD under examination.accepted by M. Katori 相似文献
18.
Firdows Loonat George Jimboyeka Amabeoku 《African journal of traditional, complementary, and alternative medicines》2014,11(3):173-181
Background
Ruta graveolens has been used to treat toothache, earache, rheumatism and fever with little scientific evidence corroborating these uses.Materials and Methods
The leaf methanol extract of Ruta graveolens was evaluated for antinociceptive activity using the acetic acid writhing and hot-plate tests in mice, also anti-inflammatory and antipyretic activities using the carrageenan-induced oedema and E. coli-induced pyrexia tests in rats, respectively.Results
R. graveolens (100 mg/kg, i.p.), significantly reduced the number of acetic acid-induced writhes by 54 %. R. graveolens (400 mg/kg, i.p.), significantly delayed the reaction time in mice to thermal stimulation 15, 30, 45, and 60 min after treatment. Combined treatment of the lowest and sub-effective doses of the leaf methanol extract (25 mg/kg, i.p.), and indomethacin (10 mg/kg, i.p.) significantly reduced the number of acetic acid-induced writhes in mice. The leaf methanol extract of R. graveolens (50 – 400 mg/kg, i.p.), significantly reduced carrageenan-induced oedema over the 4 h period of testing. Combined treatment of the lowest doses of R. graveolens (25 mg/kg, i.p.) and indomethacin (2 mg/kg, i.p.) produced a significant reduction in carrageenan-induced oedema over the 4 h period of testing. R. graveolens (100 -400 mg/kg, i.p.) significantly reduced E. coli-induced pyrexia over the 5 h period of testing. Given together, the lowest dose of R. graveolens (25 mg/kg, i.p.) and pentoxifylline (10 mg/kg, i.p.) produced a significant reduction in pyrexia induced by E. coli (50 µg/kg, i.m.) over the 5 h period of measurement. The LD50 value obtained for R. graveolens was greater than 4000mg/kg (p.o), suggesting that the plant species may be safe in or nontoxic to mice.Conclusion
The data obtained indicate that R. graveolens has antinociceptive, anti-inflammatory and antipyretic activities, justifying the use of the plant species by traditional medicine practitioners in the management and treatment of pain, inflammation and fever. 相似文献19.
Dipyrone (Dp), 4-aminoantipyrine (AA), and antipyrine (At) delay liquid gastric
emptying (GE) in rats. We evaluated adrenergic participation in this phenomenon
in a study in male Wistar rats (250-300 g) pretreated subcutaneously with
guanethidine (GUA), 100 mg·kg−1·day−1, or vehicle (V) for
2 days before experimental treatments. Other groups of animals were pretreated
intravenously (iv) 15 min before treatment with V, prazosin
(PRA; 1 mg/kg), yohimbine (YOH; 3 mg/kg), or propranolol (PRO; 4 mg/kg), or with
intracerebroventricular (icv) administration of 25 µg PRO or V.
The groups were treated iv with saline or with 240 µmol/kg Dp,
AA, or At. GE was determined 10 min later by measuring the percentage of gastric
retention (%GR) of saline labeled with phenol red 10 min after gavage. %GR
(mean±SE, n=8) indicated that GUA abolished the effect of Dp (GUA
vs V=31.7±1.6 vs 47.1±2.3%) and of At
(33.2±2.3 vs 54.7±3.6%) on GE and significantly reduced the
effect of AA (48.1±3.2 vs 67.2±3.1%). PRA and YOH did not
modify the effect of the drugs. %GR (mean±SE, n=8) indicated that
iv, but not icv, PRO abolished the effect
of Dp (PRO vs V=29.1±1.7 vs 46.9±2.7%) and At
(30.5±1.7 vs 49±3.2%) and significantly reduced the effect of
AA (48.4±2.6 vs 59.5±3.1%). These data suggest activation of
peripheral β-adrenoceptors in the delayed GE induced by phenylpyrazolone
derivatives. 相似文献
20.
WKM Abotsi GK Ainooson E Woode 《African journal of traditional, complementary, and alternative medicines》2012,9(1):138-152
Various parts of the perennial herb Hilleria latifolia (Lam.) H. Walt. (Family: Phytolaccaceae) are used in Ghanaian traditional medicine for the treatment of several inflammatory-related disorders. The present study examined the anti-inflammatory effect of an ethanolic extract of the aerial parts of Hilleria latifolia (HLE) in acute and chronic inflammation models. Since free radicals and reactive oxygen species are implicated in inflammatory diseases, the antioxidant potential of HLE was also investigated in in vitro experimental models. HLE (10–300 mg kg−1, p.o.), either preemptively or curatively, significantly inhibited carrageenan-induced foot oedema in 7-day old chicks. Similarly, the NSAID diclofenac (10–100 mg kg−1, i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3–3 mg kg−1, i.p.) dose-dependently reduced the oedema in both pre-emptive and curative treatments. In the Freund''s adjuvant induced-arthritis model in rats, HLE as well as the positive controls, dexamethasone and methotrexate, showed significant anti-arthritic properties when applied to established adjuvant arthritis. HLE (10–300 mg kg−1, p.o.) significantly reduced oedema in the ipsilateral paw of rats but failed to prevent systemic arthritic spread. The DMARD methotrexate (0.1–1 mg kg−1, i.p.) and dexamethasone (0.3–3 mg kg−1, i.p.) reduced significantly the total polyarthritic oedema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals. The extract (0.03–1.00 mg ml−1) exhibited Fe3+ reducing activity, scavenged DPPH and prevented lipid peroxidation. These findings suggest that the extract exerts in vivo anti-inflammatory activity after oral administration and also has antioxidant properties which may contribute to its activity. 相似文献