TSH IRMA,FT3,FT4RIA的临床应用及其评价

本文报道了138例初诊甲亢患者,48例甲减和60例慢性肾功能衰竭病人血清TT_3、TT_4、FT_3、FT_4(RIA)及TSH-IRMA的测定值。甲亢患者FT_3、FT_4、TSH值分别为30.82±14.50、53.08土18.03pmol/L,TSH为0.27±0.69mIU/L。与对照组比较有极显著性差异(p<0。001),甲减病人与对照组比较,FT_3、FT_4、TSH有极显著性差异(p<0.001);慢性肾功能衰竭病人与对照组间FT_4有极显著性差异(p<0.001),TT_4、TT_3有显著性差异(p<0.01)。我们认为TSH-IRMA、FT_3、FT_4测定值对诊断甲亢、甲减以及慢性肾功能衰竭的诊断均有重要价值。     

On periodic sweating from the human skin during rest and exercise

The rhythmic variations in the water evaporation rate (gm ² h ¹1 from the dorsal side of the forearm have heen studied. The evaporaition rate is estimated by calculating the difference between the vapour pressures at two distinet points on a line perpendicular to the evaporative surface. Using autocorrelation technique it is found that the evaporation rate is periodie with a period of around 0.7 s. This value is consistent. al rest as well as prior to profuse sweating in exercise. for all the subjects included in the investigation. During profuse swesting the period becomes slightly longer. It is suggested that the variations in the evaporation rate reflect the periodie activity in the sudomotor nerves.     

肠系膜上动脉闭塞性休克过程中一氧化氮的抗肺损伤用

本实验采用兔肠系膜上动脉闭塞（ＳＭＡＯ）性休ｋｅ模型，测定了休克前后人．出肺血一氧化氮（ＮＯ）的含量，动态观察了ＮＯ合成抑制剂ＬＮＮＡ对休克时平均动脉血（ＭＡＰ）和肺动脉压（ＰＭＰ）及对生存时间的影响，并测定了人、出肺血脂质过氧化代谢产物丙二醛（ＭＤＡ）的含量改变。结果发现，ＳＭＡＯ休克的出肺血ＮＯ显著低下人肺血（Ｐ＜０．００１），而休克后人、出肺血ＮＯ无明显差异，且与休克前相比也无明显差异。注入Ｌ－ＮＮＡ后显著增加了休克后的ＰＭＰ，缩短了存活时间。并使人、出肺皿ＭＤＡ含量明显增加，结果提示：在兔ＳＭＡＯ休ｋｅ中内源性ＮＯ具有重要的抗肺动脉压增高和抗肺损伤的作用。     

外源性糖皮质激素对大鼠下丘脑─垂体─肾上腺─胸腺轴的影响

本文观察大鼠每天皮下注射皮质酮（1~50mg/kg，连续14天）后下丘脑─垂体─肾上腺─胸腺（HPAT）轴的形态与机能改变。结果表明：实验大鼠垂体，肾上腺，胸腺重量减轻，下丘脑单胺类递质含量升高，下丘脑室旁核促肾上腺皮质素释放因子（CRF）分泌细胞及正中隆起CRY神经纤维和垂体前叶促肾上腺皮质激素（ACTH）分泌细胞等数量减少，染色变淡，血浆皮质酮（CORT）和ACTh浓度降低。淋巴细胞增殖反应及自然杀伤细胞活性减弱，T淋巴细胞产生IL-2、IFN－γ能力下降。提示：（1）外源性糖皮质激素剂量依赖性反馈抑制HPAT轴；（2）与此同时激活下丘脑单胺类递质，进一步支持糖皮质激素与儿茶酚胺共同构成HPAT重要调节因素的观点。     

A paper radioimmunosorbent test (PRIST) for the detection of larva-specific antibodies to toxocara canis in human sera

A paper radioimmunosorbent test (PRIST) was shown to be sensitive and reproducible when used with excretory/secretory antigen of Toxocara canis second stage larvae. Whatman No. 50 filter paper (5 mm discs) gave the most consistent and clear results with antigen at a concentration of 100 μg/ml, and could be stored for up to 3 weeks in vacuo at ?70°C. Antigen coated discs were incubated with test sera at 1 : 10 dilution for 3 h at room temperature (21°C), reacted with [¹²⁵I]anti-human IgG for 1 h and counts determined in a gamma counter. Sera from patients with fascioliasis, taeniasis, schistosomiasis, oxyuriasis, trichinellosis and ancyclostomiasis gave counts similar to cord serum controls. Sera from patients with ascariasis gave counts of up to twice as great as controls, but sera from patients with toxicariasis produced counts of 7,000–13,000, a 4–6-fold increase.     

Improved method for the isolation of purified mouse peritoneal macrophages

Mouse peritoneal macrophages were cultured for 45 min in medium supplemented with fetal calf serum (FCS) in petri dishes coated overnight with heat-inactivated FCS. After removal of non-adherent cells by washing, adherent cells were detached by a brief incubation in the presence of sub-toxic levels of ethylenediamine tetraacetate (EDTA). Overall peritoneal macrophage recoveries of 90% can be routinely achieved with this method, and full cell viability is maintained.     

Early histogenesis in the mouse cerebral cortex: a Golgi study

The early histogenesis in the cerebral cortex of the mouse was studied with a modification of the rapid Golgi method. Three stages can be distinguished first, a period during which the telencephalon is exclusively populated with neuroepithelial cells (till E12); secondly the transient stage of the primordial cortical organization, characterized by the presence, in the outer part of the neural tube, of a loose network of primitive horizontal nerve cells and third, the appearance of the early cortical plate (E14) which contains closely packed, radial bipolar nerve cells. In addition to some salient morphological features of the various cell types, the technique also reveals the importance of efferent fibers from the cortex during the early cortical development.     

解剖学教学中做好中差生转化的点滴体会

近年来 ,成人中专学生知识基础普遍较差 ,要教好卫生中专的医学基础课难 ,尤其是解剖组织胚胎学更难 ,促进中差生的转化更加难。在多年来的解剖学教学工作中 ,作者在努力转化中差生方面做了一些尝试 ,在此略谈几点体会。根据卫生成人中专学生的实际情况和教学大纲的要求 ,在解剖学教学中做好中差生转化工作必须努力做到“四个坚持、四个防止”。1　坚持循序渐进 ,防止急躁成人中专教学对象主要是中差生 ,基础不牢固对新接触的学科影响不大 ,但接受、理解能力比较有限 ,要在一学期多内完成解剖组织胚胎学的所有教学内容 ,任务艰巨。越是这样 …     

自发性高血压鼠心肌内去甲肾上腺素的变化

运用高效液相色谱电化学检测仪测定24只自发性高血压鼠(SHR)心肌内去甲肾上腺素(NA)的含量。SHR分成3个月与6个月龄两组,并与同龄组的正常血压京都种大白鼠(WKY)作比较。结果在3个月龄组的SHR心肌内NA含量低于WKY,而6个月龄组的SHR则高于WKY,两者都有显著性差异。同时亦测量了它们的血压变化。     

抗人肝癌导向药物的制备及其分析

本文报道了肝癌单克隆抗体Ａ２４－表阿霉素结合物的制备及其对肝癌细胞ＳＭＭＣ－７７２１的体外杀伤作用。先用高碘酸钠把葡聚糖氧化成多醛基葡聚糖，再以此为中介体连接单抗与表阿霉素。结合物中抗体与药物摩尔比为１：４０。单抗活性保存良好。该结合物对肝癌细胞的毒性显著高于游离药物及无关抗体结合物，而对非靶细胞的毒性则明显低于游离药物。说明结合物对肝癌细胞有较强的选择性杀伤作用，有可能用于肝癌导向治疗。     

Detrusor and uterine cervix electromyography

Electromyographic studies of various smooth muscle organs have been made by different workers to define the normal physiology and to try to elucidate the nature of some pathological conditions. High-frequency low-voltage activity has been recorded previously from both the uterus and the bladder. Low-frequency high-voltage activity recorded from the bladder and from the gastrointestinal tract has been shown to correlate with different pathological disorders. The paper describes the low-frequency high-voltage electromyographic activity, previously unreported, which can be recorded from the cervix uteri, and discusses the means of computer frequency analysis in the quantisation of such signals.