幽门螺杆菌感染与胃癌PCNA P53基因突变的关系

目的 研究幽门螺杆菌(Hp)感染胃癌患者增殖细胞核抗原、抑癌基因P53基因突变情况,以探讨Hp可能的致癌机制。方法 应用免疫组化法检测PCNA蛋白表达情况,PCR/SSCP方法观察P53基因第5~8外显子突变情况,快速尿素酶法和HE染色检测Hp感染情况。结果 胃癌组Hp感染21例(21/30,70%)。从浅表性胃炎到胃癌PCNA指数呈递增趋势,各组间均有显著性差异(P < 0.05)。浅表性胃炎和萎缩性胃炎组中Hp阳性患者与Hp阴性者的PCNA LI相比有显著性差异(P < 0.05),其余各组间Hp 阳性和Hp 阴性病例间的PCNA LI无显著性差异(P > 0.05)。异型增生P53基因第8外显子突变1例(1/30,3.33%),为Hp阳性者。胃癌组织P53第5~8 外显子突变17 例(17/30、56.67%),Hp 阳性胃癌组的突变率与Hp 阴性组相比有显著性差异(P < 0.05)。结论 Hp感染者具有更多肿瘤生物学行为,可以引起PCNA蛋白表达增强、抑癌基因P53突变,Hp可能为促癌剂在胃癌的发生发展中起一定作用。     

p53、bcl-2蛋白表达与胃癌生物学行为的关系

目的研究p53、bcl-2蛋白在胃癌及癌前病变中的表达与胃癌生物学行为的关系。方法采用免疫组化SP法,检测p53、bcl-2蛋白在60例胃癌、46例异型增生和20例正常胃黏膜中的表达情况。结果p53在正常胃黏膜不表达,异型增生表达率为20%,随胃黏膜异型增生程度的加重,p53表达逐渐增高,在胃癌中的阳性表达率为53%。bcl-2在胃癌中的阳性表达率为47%,在异型增生中表达率为48%,在正常胃黏膜中表达呈弱阳性。bcl-2表达与胃癌的分化程度有关(P<0.05),与淋巴结转移、浸润深度无关(P>0.05);p53表达与上述临床病理因素均无关。结论p53、bcl-2蛋白的表达与胃黏膜增殖和恶化密切相关,可作为评估胃癌预后的参考指标。     

胃癌中医证型与P53、PCNA、CerB-2表达的关系分析

目的:探讨胃癌中医证型与P53、PCNA、CerB-2表达的关系.方法:选取本院行胃癌根治性手术或姑息术治疗的胃癌患者142例,根据胃癌中医辨证分型标准,分为肝气犯胃证、胃热伤阴证、气滞血瘀证、痰湿凝结证、脾胃虚寒证、气血亏虚证共6个证型,所有患者胃癌切除组织石蜡包埋,切片,通过免疫组织化学法检测P53、PC-NA、C...     

中药复方AC_(960)对人肝癌细胞p21~(ras)、bal-2、P~(53)基因表达调控作用的研究

目的：研究中药复方ＡＣ９６０对人肝癌细胞基因、抑癌基因表达的影响,探讨其抗癌作用机制。方法：ＡＣ９６０灌胃给药后,采集大鼠血清,处理人肝癌细胞ＳＭＭＣ７７２１,３Ｈ－ＴｄＲ掺入法观察细胞增殖,流式细胞仪分析细胞周期和ｐ２１ｒａｓ、ｂａｌ－２、Ｐ５３基因蛋白表达。结果：ＡＣ９６０具有显著抑制 ＳＭＭＣ－７７２１细胞增殖和促进细胞凋亡作用,细胞周期分析,ＤＮＡ直方图上见典型凋亡峰,２４ｈ时为２１．２５％,４８ｈ时为２７．７７％,早期（２４ｈ）Ｓ期细胞比例增加,Ｇ２／Ｍ期减少,后期（４８ｈ）,Ｇｏ／Ｇ１期细胞增多,Ｓ期减少。Ｐ５３、ｂａｌ－２、ｐ２１ｒａｓ在 ＳＭＭＣ－７７２１细胞中都有不同程度表达,ＡＣ９６０处理后,Ｐ５３表达增强,ｂｃｌ－２、ｐ２１ｒａｓ表达降低。结论：抑制癌基因、增强抑癌基因表达,阻断细胞增殖周期,抑制人肝癌细胞增殖,促进癌细胞凋亡可能是ＡＣ９６０抗肝癌主要作用机制。     

扶正抗癌汤对肝癌小鼠突变型p53基因和巨噬细胞CD_(68)表达的影响

目的:通过实验研究扶正抗癌汤对H22模型小鼠的抑瘤率、突变型p53基因及巨噬细胞CD68的影响,探讨其抑瘤率,提高机体免疫功能的机理。方法:50只小鼠,随机抽取10只作为正常对照组(A),其余40只采用每只右腋皮下接肿瘤细胞悬液0.2mL再随机分成4组,每组10只,分别设为:模型对照组(B),替加氟组(C),扶正抗癌汤大剂量组(D),扶正抗癌汤小剂量组(E)。于接种瘤细胞悬液24h候开始给药,连续给药14d,经口灌胃14d后,取瘤体称重,计算抑瘤率;用免疫组化法检测各组突变型p53基因和巨噬细胞表达。结果:扶正抗癌汤各组与模型组相比肿瘤体积缩小,扶正抗癌汤大剂量组对H22模型小鼠的抑瘤率为33.32%,用药两周扶正抗癌汤大小剂量组各治疗组瘤组织p53表达显著下降,其中替加氟组及扶正抗癌汤大剂量组与模型对照组比较具有非常显著性差异(P<0.01);巨噬细胞CD68检测结果示:扶正抗癌汤大剂量组与模型对照组比较,具有极其显著性差异(P<0.01)。结论:扶正抗癌汤特别是大剂量组对H22肝癌型小鼠肿瘤有一定的抑瘤作用。扶正抗癌汤大剂量组在抗肿瘤下调抑制细胞凋亡的突变型P53基因同时促进巨噬细胞CD68的表达。     

针刺对胃血流的影响及其神经机制探讨

<正> 临床上针刺具有调整脾胃功能,治疗胃肠疾患的疗效。有关实验工作除了早期有针刺影响胃运动和胃液分泌的报道之外,近年来针刺影响胃电活动的报道较多。然而胃血流和胃液分泌紧密相关,并在胃疾病的发生机理方面起着重要作用。针刺对胃血流的影响如何,至今未见报道。本工作研究针刺对胃血流的影响及其神经机制,并就电针和手针对胃血流的影响,相应的胃液分泌及胃电变化进行了分析比较。     

REVIEW ON THE CLINICAL RESEARCH DEVELOPMENT IN ACUPUNCTURE AND MOXIBUSTION

SincethefirstInternationa1AcupunctureandMoxibustionAssociationwassetup1oyearsago,clinicalresearchofacupunctureandmoxi-bustionhasdevelopedonanunprecedentedspeed.Especially,theapplicationoftechniquesinotherfieldsbroughtthesubstantialbreak-throughinacupunctu…     

驳骨灵口服液对绝经后骨质疏松症患者血清瘦素、一氧化氮及白介素-6的影响

目的：探讨驳骨灵口服液治疗绝经后骨质疏松症的作用杌理。方法：选择绝经后骨质疏松症患者30例，观察测定其治疗前及治疗6个月后血清中瘦素（Leptin）、一氧化氮（NO）、白介素-6（IL-6）、骨钙素（BGP）、雌二醇（E）含量以及L2-4椎体正位骨密度（BMD）。结果：患者经驳骨灵口服液治疗6个月后，血清中NO、E2、Leptin水平均明显上升（P〈0．01），IL-6、BGP水平均明显下降（P〈0．01）。骨密度（BMD）治疗前后比较，差异无统计学意义（P〉0．05）。结论：驳骨灵口服液为治疗绝经后骨质疏松症的一个有临床应用价值和开发价值的药物。     

EFFECT OF ELECTROACUPUNCTURE OF "ZUSANLI" ON VIP CONTENTS OF THE PERIPHERAL BLOOD, GASTRIC MUCOSAL AND BRAIN TISSUES IN GASTRIC MUCOSAL INJURY RATS

Ｉｔｉｓｗｅｌｌｋｎｏｗｎｔｈａｔｖａｇａｌｎｅｒｖｅｉｓｏｎｅｏｆｔｈｅｐｒｉｎｃｉｐａｌｆａｃｔｏｒｓｆｏｒｉｎｄｕｃｉｎｇｉｍｂａｌａｎｃｅｂｅ ｔｗｅｅｎｔｈｅｉｎｊｕｒｙｆａｃｔｏｒｓａｎｄｐｒｏｔｅｃｔｉｏｎｆａｃｔｏｒｓｏｆｔｈｅｇａｓｔｒｉｃｍｕｃｏｕｓ.Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ａｌｏｎｇｗｉｔｈｔｈｅｐｒｏｐｏｕｎｄｉｎｇｒｅｓｅａｒｃｈｏｎｉｎｔｅｓｔｉｎａｌｎｅｒｖｅｓｙｓ ｔｅｍ ,ｔｈｅｉｎｃｒｅａｓｉｎｇｆａｃｔｓｄｉｓｐｌａｙｔｈａｔｐｅｐｔｉｄｅｒｇｉｃｎｅｕｒｏｍ…     

OBSERVATION ON CLINICAL THERAPEUTIC EFFECT OF MRI-AIDED ENCLOSURE NEEDLING IN THE TREATMENT OF STROKE AND ITS INFLUENCE ON BLOOD RHEOLOGY

Objective: To observe clinical therapeutic effect of magnetic resonance imaging (MRl)-aided enclosure needling in the treatment of stroke patients and changes of the related blood rheology. Methods: A total of 61stroke patients were randomized into MR I-aided enclosure needling group (MRI-aided acupuncture group) (n = 31 ) and conventional acupuncture group (n= 30). For patients of MRl-aided acupuncture group, acupuncture needles were inserted into the subcutaneous tissues around the focus-projection scalp area displayed by MRI, with the needle tips toward the center of the projection region and with two needles being about 2 crn apart, combined with other acupoints according to the concrete syndromes or symptoms. For patients of conventional acupuncture group, Motor Area (MS 6)and Sensory Area (MS 7) on the contralateral side of the focus were punctured. The treatment was conducted once daily, continuously for 30 days. Results: After treatment, of the 31 cases in MRl-aided acupuncture group, 20 werecured basically, 10 responded with significant improvement of symptoms and signs, one case had some improvement.While in conventional acupuncture group, of the 30 cases, 11 were cured basically, 15 responded with striking amelioration of symptoms and signs and 4 had some amelioration. Ridit analysis showed that the therapeutic effect of MRI-aided acupuncture group was significantly superior to that of conventional acupuncture group (P ＜ 0.05). After treatment,the whole blood viscosity, plasma viscosity, hematocrit, whole blood reduction viscosity, hemagglutination index, and the total score of the two groups all decreased significantly in comparison with those of pre-treatment of each group,while whole blood viscosity, hematocrit, vascular sclerosis index and the total score of MRl-aided acupuncture group were obviously lower than those of conventional acupuncture group ( P＜ 0.05- 0.01 ), suggesting that the effect of theformer group in bettering blood rheology was pronouncedly superior to that of conventional acupuncture group. Conclusion: MRl-aided acupuncture group is obviously superior to that of conventional acupuncture group in improving clinicalsymptoms and signs and blood rheology in stroke patients.     

辨证选用活血舒筋手法治疗肩凝症的临床研究

为系统评价活血舒筋手法治疗肩凝症的临床疗效和安全性。初步探讨其治疗机理。选择90例肩凝症患者,随机分为活血舒筋手法治疗组、针刺治疗组和扶他林治疗组。在相同功能锻炼的情况下,分别给予相应治疗,疗程为2周。治疗前后观察患者临床症状分值变化、临床疗效和安全性评价。结果显示7天后手法治疗组临床疗效明显高于针刺治疗组,而与扶他林组差异无统计学意义。14天后手法治疗组和扶他林组显效率明显高于针刺治疗组,两组间差异无统计学意义;手法治疗组总有效率高于其余两组;三组均无不良反应发生。表明手法、针灸、外用扶他林治疗肩凝症均有效,但手法治疗疗效更好,活血舒筋手法治疗肩凝症具有良好的安全性和有效性。     

A CLINICAL STUDY AND MECHANISM RESEARCH OF PRICKING BLOOD CUPPING THERAPY ON CERVICAL SPONDYLOSIS RADICULOPATHY

Objective: To probe into clinical effects of pricking blood cupping for treatment of cervical spondylosis radiculopathy, and to interpret part of the mechanism of pricking blood cupping from the point of view of local microcirculation.Methods: Five-center, single-blind, randomized controlled method was used.A total of 183 cases were divided into pricking blood cupping group of 68 cases, acupuncture group of 56 cases, and pricking blood cupping plus acupuncture group of 59 cases, using the random number table.They were evaluated by VAS, NDI, and clinic evaluation scale.Forty cases were chosen inpricking blood cupping group and acupuncture group, and their blood perfusion changes of Dazhui and Jianjing(affected side) were recorded by laser speckle system.Results: VAS, NDI, and clinic evaluation scale scores were decreased(P0.01) after one, two, and three courses of treatment in all the three groups.There was no significant changes among the three groups(P0.05).Blood perfusion of Dazhui and Jianjing rose immediately(P0.05) after treatment in both two groups, and changes in pricking blood cupping group were more obvious(P0.05).Conclusion: Pricking blood cupping and acupuncture both could release pain, and improve life quality of patients with cervical spondylosis radiculopathy obviously, due to a better immediate blood perfusion after the treatment of pricking blood cupping.     

散瘀接骨精在四肢骨关节损伤康复中的应用

为观察散瘀接骨精在四肢骨关节损伤康复中应用的疗效,将208例由于四肢骨关节损伤所致的组织粘连和瘢痕形成引起的陈旧性伤痛患者分两组治疗。对照组99例,在医师指导下行功能训练、物理治疗等常规处理;治疗组109例,加用散瘀接骨精以手法在损伤部位反复按揉20分钟,每天2次,7～10天为1个疗程;分别观察两组病人用药前后疼痛、肿胀、压痛、功能障碍等症状体征。均于治疗2个疗程后第4周观察疗效,结果治疗组显愈率80.7%,总有效率99.1%,对照组显愈率31.3%,总有效率82.8%,两组疗效差异有显著性。表明散瘀接骨精在四肢骨关节损伤康复治疗中效果显著。     

CLINICAL OBSERVATION ABOUT THE EFFECT OF BLOOD-LETTING OF JING-POINTS ON CEREBRAL BLOOD FLOW IN STROKE PATIENTS AT THE EARLY STAGE AND EXPERIMENTAL STUDY ON ITS MECHANISMS IN THE RABBIT

Ｃｕｒｒｅｎｔｌｙ ,ｉｎｔｈｅｔｒｅａｔｍｅｎｔｏｆｓｔｒｏｋｅ(ｃｅｒｅｂｒａｌａｐｏｐｌｅｘｙ) ,ｍｏｓｔｐｅｏｐｌｅｐａｙｍｏｒｅａｔ ｔｅｎｔｉｏｎｔｏｔｒｅａｔｍｅｎｔｏｆｔｈｅｓｅｑｕｅｌａｅ ,ｂｕｔｌｅｓｓｉｓｐａｉｄｔｏｔｈｅｅｍｅｒｇｅｎｃｙｔｒｅａｔｍｅｎｔｒｅｍｅｄｉｅｓｆｏｒｓｔｒｏｋｅａｔｔｈｅｅａｒｌｙｓｔａｇｅ .Ｍｏｓｔｓｔｒｏｋｅｐａｔｉｅｎｔｓｄｉｅｏｒｇｅｔｓｅｑｕｅｌａｅｄｕｅｔｏｄｅｌａｙｅｄｏｒｅｒｒｏｎｅｏｕｓｅ ｍｅｒｇｅｎｃｙｔｒｅａｔｍｅｎｔａｎｄｔ…     

CLINICAL RESEARCH ON THE EFFECT OF ACUPUNCTURE ON THE LEFT VENTRICULAR CONTRACTION FUNCTION IN SYMPTOMLESS CARDIAC FAILURE PATIENTS

Objective: To observe the effect of acupiJmtlxe therapy on the function of myocardial contraction in symptomless cardiac failure patients. Methods: A total of 60 cases of svmDtomless cardiac insufficiencv inoatients were randomly divided into medication and acupuncture groups, with 30 cases in each group. For patients of aoulomcbxe group, Neiguan (PC 6), Ximen (PC 4), Tanzhong(CV 17), Xinshu (BL 15), Jueyinshu (BL 14) and Geshu (BL 17) were punctured, twice daily, with 30 days being a therapeutic course. In medication group, patients were asked to take Capoten 25 mg (t. i. d. ). The left ventricular ejection fTaction (LVEF) and its fracticnal shortening (FS) of the minor axis were used as the indexes for assessing the therapeutic effect and detected by using a color ultras~ic Doppler apparatus. Results: After one course of treatment, of the each 30 cases of actCucture and medication groups, 13 (43.3%) and 16 (53.3%) had marked improvement, 16 (53.3%) and 14 (46.7%) had improvement, and 1 (3.3%) and 0 had no any effect respectively. No significant difference was found between two groups in the therapeutic effect. It shows acupuncture therapy can improve myocardial contraction function. Conclusion: Acuounclure has a similar effect in enhancing the systolic function of the myocardium.